LOCUS       sequence-449882-        7994 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor IRF4 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7994)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 7994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-IRF4-Brd 64
FEATURES             Location/Qualifiers
     source          1..7994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             161..341
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(342..568)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(596..616)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(596..614)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(635..651)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(635..651)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(648..670)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    659..675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(683..689,690..707,708..713))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    728..749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(866..883)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1037..1625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1117..1136)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1796..2587,2588..2656))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2419..2438
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2657..2761)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2787..3242)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2924..2945)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3136..3155
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3369..3391
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3383..3400
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3384..3400
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3410..3429
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3410..3428
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3449..3584
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3624..3745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3635..3654)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3689..3708
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3817..4050
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4169..4572
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     promoter        complement(6204..6610)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    6644..6761
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7028..7072)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7257..7490
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    join(7983..7994,1..114)
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cccatctctc tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg
       61 cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc
      121 tggtttccct ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact
      181 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
      241 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
      301 gagagctccc aggctcagat ctggtctaac cagagagacc cgtttatgta tcgagctagg
      361 cacttaaata caatatctct gcaatgcggc aattcagtgg ttcgtccaat ccatgtcaga
      421 cccgtctgtt gccttcctaa taaggcacga tcgtaccacc ttacttccac caatcggcat
      481 gcacggtgct ttttctctcc ttgtaaggca tgttgctaac tcatcgttac catgttgcaa
      541 gactacaaga gtattgcata agactacatt aagcttgcag ctccagcttt tgttcccttt
      601 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      661 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      721 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      781 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      841 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      901 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      961 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1021 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1081 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1141 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1201 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1261 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1321 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1381 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1441 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1501 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1561 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1621 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1681 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1741 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1801 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1861 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1921 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1981 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2041 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2101 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2161 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2221 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2281 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2341 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2401 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2461 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2521 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2581 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2641 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2701 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2761 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     2821 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     2881 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     2941 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     3001 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     3061 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     3121 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     3181 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     3241 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     3301 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     3361 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     3421 actatagggc gaattgggta cgtccctagg cctccaaaaa agcctcctca ctacttctgg
     3481 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     3541 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     3601 gggatagcta gagccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     3661 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     3721 cattataagc tgcaataaac aagttcctct cactctctga tattcatttc tttgcaagtt
     3781 ataaatactg aataataaga tgacatgaac tactactgct agagattttc cacactgact
     3841 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
     3901 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
     3961 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcagatctt
     4021 gtcttcgttg ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa
     4081 gatctacagc tgccttgtaa gtcattggtc tagaagcgct aactgggttg tacatctaga
     4141 aggtacagct tgatatcgaa ttgcaaagga cgtcagcttc cccgacaaca ccacggaatt
     4201 atcagtgccc agcaacctag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc
     4261 tgccgtggcg atcgggaggg ggaaagcgaa agtcccagaa aggagttgac aggtggtggc
     4321 aatgccccag ccagtggggg ttgcgtcagc aaacacagag cacaccacgc cacgttgacg
     4381 gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa
     4441 aacgaaagcc gtacgggaag caatagcatg atacagaggc attaaagcag catatccaca
     4501 cagcgtaaaa ggagcaacat agttaagaat atcagtcaat ctttcacaaa ttttgtaatc
     4561 cagaggttga ttaacaggaa cagagcgtaa ataacgggaa agtttcttaa catgtttgtc
     4621 ttgtggcaat acacctgaac tagtaattac atatccctaa aaatgtaaat gattgcccca
     4681 ccattttgtt ttattaacat ttaaatgtat acccaaatca agaaaaacag aacaaatatg
     4741 ggaataaatg gcggtaagat gctcgaattc ctgcagcccg ggggatccga ccggttcatt
     4801 cttgaataga ggaatggcgg atagatctgt ggtaatcttc tggattgctg atgtgttctg
     4861 gtaaatcgta gcccctcagg aaatgtccac tgttttgttg agcaaaataa tatagttgtc
     4921 tggctagcag aggttctacg tgagctgtga tgagctttct ttgcctctga gggtctggaa
     4981 actcctctcc aaagcataga gtcacctgga atcttggcag ggagcggccg tggtgagcaa
     5041 acgcttgcag ctctgacaag aactgctgtg tgtcaaagag cttgcaggtc tggtctctct
     5101 ccagtttgtt gggccggtcg ttgcacagcg ccaggggccc gtcccagtag atcctgctct
     5161 ggcacagtct tttcgcatag agcccgtcgg gggccatcca gaggaccacg cccctctcca
     5221 ggtggctcag cagcttctca atgtttttcc tctggccatt gtcctctggg taggggaaca
     5281 ggacctggtc caggttgctg gcgtcatacg tatgtccatg ggagatccgg cagccctcgg
     5341 ggctggacgt ggtcagctcc ttcacgagga tttcccggta gtacaggcag atgtgcagcc
     5401 ggcagtctga gaacgccaag gcttcggcag accttatgct tggctctgtg gggactccgg
     5461 gagcctggga ctcaggtggg gcacaagcat aaaaggttcc tgtcacctgg caaccatttt
     5521 cacaagctgg gccttgccag tggtggccgc ggggtccaaa cgtcatggga cattggtacg
     5581 ggatttccgg gtgtggctga tccgggacgt agtccctcca gcttcggtcg aggggtggca
     5641 tcatgtagtt gtgaacctgc tgggctggga gcgaagggta aggcgttgtc atggtgtagg
     5701 ggtggctcat ggacatctgc gggtcctcca aggtgagctg cttggctcct tttttggctc
     5761 cctcaggaac aatcctgtac actttgtacg ggtctgagat gtccagctgg ctccgctcaa
     5821 ccagttcctc aaagtcattg ctcttgttca aagcgcaccg caggcgcgtc ttccaggtgg
     5881 gagggtccgg cttgtcgatg ccttctcgga actttccttt aaacagtgcc caagccttga
     5941 agagcgcggc gtcctcctcg cggttgtagt cctgcttgcc cgcgtgcttc caggggatgc
     6001 ggaagatgct cttctcctcg ttctcccaca ccagcccggg gtacttgccg ctgtcgatct
     6061 ggtcgatcag ccactggcgg agcttcccgt tgccgcagct caccgcgctc atgccgaact
     6121 ctccgcctcg gccgccgccc tccaggttca tttccaaccg cgggctagcg gatccgtcga
     6181 cggtatcgat aagcttgatt cgaccggccg actcagtctg tcggaggact ggcgcgccga
     6241 gtgaggggtt gtgagctctt ttatagagct cgggaagcag aagcgcgcga acagaagcga
     6301 gaagcaggct gattggttaa ttcaaataag gcgcagggtc atttcaggtc cttgggggag
     6361 cctggaaaca tctgatgggt cttaagaaac tgctgagggt tgggccatat ctggggacca
     6421 tctgttcttg gcctcgggcc ggggccgaaa ctgcggtgac catctgttct tggccccggg
     6481 ccggggccga aactgctcac cgcagatatc ctgtttggcc caacgttagc tattttcatg
     6541 tacccgccct tgatctgaac ttctctattc ttggtttggt atttttccat gccttgcaaa
     6601 atggcgttac tgcagccagc tcgaggctag tctcgtgatc gataaaattt tgaatttttg
     6661 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
     6721 cccctgcact gtacccccca atcccccctt ttcttttaaa agttaaccga taccgtcgag
     6781 atccgttcac taatcgaatg gatctgtctc tgtctctctc tccaccttct tcttctattc
     6841 cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat
     6901 atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt aaacctacca
     6961 agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt atgttaaacc
     7021 aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc ttttcttgct
     7081 ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta atttctctgt
     7141 cccactccat ccaggtcgtg tgattccaaa tctgttccag agatttatta ctccaactag
     7201 cattccaagg cacagcagtg gtgcaaatga gttttccaga gcaaccccaa atccccagga
     7261 gctgttgatc ctttaggtat ctttccacag ccaggattct tgcctggagc tgcttgatgc
     7321 cccagactgt gagttgcaac agatgctgtt gcgcctcaat agccctcagc aaattgttct
     7381 gctgctgcac tataccagac aataattgtc tggcctgtac cgtcagcgtc attgacgctg
     7441 cgcccatagt gcttcctgct gctcccaaga acccaaggaa caaagctcct attcccactg
     7501 ctcttttttc tctctgcacc actcttctct ttgccttggt gggtgctact cctaatggtt
     7561 caatttttac tactttatat ttatataatt cacttctcca attgtccctc atatctcctc
     7621 ctccaggtct gaagatcagc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct
     7681 tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa
     7741 tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat
     7801 ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta
     7861 actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt atattttttc
     7921 tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata
     7981 ctgacgctct cgca
//
