LOCUS       sequence-450056-       14070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of ApyA peptide modified by the B12-rSAM enzyme ApyD 
            (optional, refer to publication), the cytochrome P450 enzyme ApyO, 
            and the MNIO enzyme ApyHI, and the methyltransferase ApyS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14070)
  AUTHORS   Nguyen DT, Zhu L, Gray DL, Woods TJ, Padhi C, Flatt KM, Mitchell DA,
            van der Donk WA
  TITLE     Biosynthesis of Macrocyclic Peptides with C-Terminal 
            beta-Amino-alpha-keto Acid Groups by Three Different Metalloenzymes.
  JOURNAL   ACS Cent Sci. 2024 Apr 11;10(5):1022-1032. doi: 
            10.1021/acscentsci.4c00088. eCollection 2024 May 22.
  PUBMED    38799663
REFERENCE   2  (bases 1 to 14070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscentsci.4c00088"; journalName: "ACS Cent Sci"; date: 
            "2024-04-11- 11"; volume: "10"; issue: "5"; pages: "1022-1032"
COMMENT     Sequence Label: pSCrhaB2_ApyOAHIDS
FEATURES             Location/Qualifiers
     source          1..14070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..119
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             1704..1721
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6779..6796)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6949..7035
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7127..7154
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(7620..8282)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      8283..9054
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     primer_bind     10312..10330
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10447..10549
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(10616..10635)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(11505..11524)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(11548..11784)
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     promoter        complement(join(12110..12115,12116..12132,12133..12138))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             complement(12167..13015)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(13089..13925)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
ORIGIN
        1 caccacaatt cagcaaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc
       61 ccattttcct gtcagtaacg agaaggtcgc gaattcaggc gctttttaga ctggtcgtaa
      121 tgaaattcag caggatcaca taatgaacgg tctacctccg tcgcttccgc gggtcgacag
      181 caccgagtcg ctttttgcgg agccgctcgc gtttctcgca caagcgcgct cgcggcatgg
      241 tgatgtcttc gtcatgcggg agcatggtcc gattttttcc cgcgcgagcg attgcagcgg
      301 cgtgatagcg gtattcggcg agcatcggct tcgacagata ttgaccgata tagacaattt
      361 cgcgttgccg atgtccgcgg cggcgaaaat ggcattgccg aaaaacctcg tcaacctcaa
      421 tcgcggcttg cacagcatgc gcgagccgga gcatggccgg cacaagcgcc tcttgacggg
      481 aacgatcaat cgcgagttgt tcgacgcgca ccgcttcgaa atacgagcgg ctttgaaccg
      541 cttttgcgaa atgctgaaag tggaccgccg gatttcagtg gtcagccgga tgcgcgagct
      601 gaccgtggag atggcgtctc atatctttct tggggcgcag tgccaagagg atgacgaact
      661 ggcgtttctt ttaagcgcct attttacttt gcgacgcgaa gcgtcttcgc tgaacgcgcg
      721 cgatcccctg ctgtaccggg acgaactgat cggcgtcggg cagcaactcg accgcacatt
      781 gcgggagcgg atcaggcggt atcgaaagcg tcccgtcgac gctcgtgcgg ggctgctgca
      841 acgtctggca acggccggac cgccgggctc gcccgcgctt tccgaggacg agatagtcgg
      901 ccatgccaac gtgatgttcg tgtcgagcac cgagcccgtc gcgatgtcgc tgacgtggct
      961 gctgctcgtt ctgtcgcagc tgcccgatct gaggcgcgcg cttcgcgcgg aaattgccga
     1021 tcgcgcgtcg atgccggcat cgacgaacgg cgcgtcgtgg ctcgagaacg tcgtcaacga
     1081 gacgctccgg ctcctgacgc ccaacgcgct gatggttcgc gcgacgacgc gcgcggtctc
     1141 gctgcaaggc gtcgcgcttc cggcgcgttg cgagatcgtc gtgtgtccgt tcctcgcgca
     1201 tcgcgaagcc aagccgtttc cggacccgca tgcattctcg ccgtcccggt gggagacggc
     1261 caggccgtct ccgtacgagt attttccgtt cggcgccggc ggtcattttt gcgcggggcg
     1321 aaatctcgcg ctctcgctga ttcgcgaagt actgtcaacg ctgctatcgc gcttcgattt
     1381 cgttttggat ggcgaacagt ctatcgactg gcgcattcat atcatgttga tgccgaaagg
     1441 cgacccggca ctcatcgcgc atcccgtcga tgagcgcggt gacacgccgt cgccgaaatg
     1501 gcgcgggccg ataaccgatc tattccattt cgcgccgggg ctttcttgac atttaccagg
     1561 gcgcatcctt taatcagaag cgaatcggca tgcatgccca tcgggatttt ttccagtcat
     1621 tgatccgcat ggttcttttc acgggttatt ccgcgctcac acgctaagcg gcgcgtcctg
     1681 cctacaacaa aggaacaatc atgcatcacc atcaccatca catggctacg aaacccaaaa
     1741 aaacaaaggg cgtttctgtt tccatcgaag ggaagctgcc caagatgacc ctggatatgc
     1801 cggtggacgc gaaaaagatc aaggccatcc agaaatgcct ggaaaacggc aagctgacca
     1861 tcacgatgag caaggtggat cttgccggcg gccgcatggg cgagggctat ctctacgact
     1921 gatgtccggc ggcgctctgg ttttcgccgg cggcgtcgcg atctcgccgc ctcgatgctc
     1981 gggcctcgga gggaacgtgc cgccgaacgc gtgcgtttgg cgcgcggtcg cgcgataacg
     2041 ggcgatgaat tccgggcttc ggcagttcga ccttaagggc gcgggaagcc tatgaaagcc
     2101 ttcgatcgcg atgcatcgga gcctcggatc ggtctcgcgt atgggcccgg gatggcggaa
     2161 ttcgccgcac ggaacgcgca cctcgtcgat tacatcgaag tgccgttcga gcaattgcga
     2221 ttctcgccgg cggtcgccga gcttcagcaa accattccgt tcgttctgca ctgcgccagc
     2281 ttgtccgtcg cgggattcgt gccgcccgac gattcgacgg tcgacgcaat cgagcgcacg
     2341 gccgtgcaga ccggcacgcc atggataggc gaacatctcg cgtacatttc ggccgatccc
     2401 gttggcgaag cgctgggcgg gaccggtgag cccacctcgt tgtcgtacac gttgtgccct
     2461 cagttgagcg acgagacggt acgccgcgtc gtcgacaatc tcgccgcgct tcgtccgcat
     2521 tttccggttc cactgatcgt cgaaaactcc ccgcagtact ttccgatacc gggcagcacg
     2581 atgggaatga cggacttcat tcgcgcgatc acggaccgat gcgacgtcgg tttgctcctg
     2641 gacctgagtc acttcctgat cacggcgcac aacacgggcg cagaggtgca tcgggaactc
     2701 gcccggcttc cgctggagcg cgtggtcgaa gttcatctgt ccggaatgag cgtgcagtcc
     2761 ggcacggcct gggacgacca ttcgctgccg gcatcgccga tcctgttcga actgctcgag
     2821 cgcctgctcg acgtggcgcg gcctcgcgcg ctgaccttcg aatacaactg gtcgccatac
     2881 ttcccgctgt cggtcctgac gacgcatatc gagcgcgcac gccagttgat ggggctcgca
     2941 tgaacgcagg gaaaatacac gccgtgatcg ccgccggcgc caaggacccg cggctcatcg
     3001 agcattggcg gcggcgtccg gacctgctgc gccgcgcagg aatcgagccg gatcatctcg
     3061 atttgcgcgt attggagaat ttcgccgggt tgagcctgaa ggtgcgtcac aacggcctgc
     3121 gcggggatct gccgctgagt ttcaggttgt tgagcgtggc cggcctcgag atcgaggtct
     3181 tcgccgcgta tgcgtcggac tgcgcggaaa gcggccatgt attcgccgcc acgccgcggg
     3241 aacgcgcgcg cgatctgatg gcgttcctcg acagatggct ggaccccgcg caacgaaatc
     3301 acgttctgct gtgggacatc cttcgtcatg aatatgcgct ttcgtgcctg cggcaagaaa
     3361 gcgccgcgcc gctacccgcc gctcgccgca tcatccgcca tcgggatcgc gcgacaggcg
     3421 gctgcattcc gatgctcaac ggctccgtcg ctcagcacgt gatgcattgc aatccggaag
     3481 acctggcgtc ggcgctgcgt cagaaggagc cgccgctcga tgcggttccc gccgccgaca
     3541 cttactgctg ctattggcgc tccgccgaaa cgggcgaaat ccggtttctg caggccgata
     3601 cgctcggcta tttcgcgatc gaagcgatcg acggccggcg atccgccgcc gacatctgcg
     3661 atcggctgct cggcggccgc cgtctgctgc cgcagttcct ccggtcgctc gaccaactgg
     3721 ccggaatcgg gatcgtgcgg ttcgagaggc cgctcggaag cgctgcggca tgaaactgct
     3781 gctcgtcgac aacctcgtca tgccggaaga gggctcgctc gcgtacctcg acgtgcatcc
     3841 gcatctcggg ctgctcgcgc tggccgcggt cgccgaagcc gacggccatc gcgtgcagat
     3901 ctacgatccg aaacgactga tcagggacgg cacgctggct tacgatgcga cgctttacga
     3961 gcgggcgagc caagcgattc tcgcggcgcg tccggacggg gtcggcttca cggcgctcgg
     4021 ctgcagcttt ctgtttgcgc tcaatgtcgc ggcgctgttg aagcggcgcg agcccgacct
     4081 gcccgtcttg ctcggcgggc cgcacgcgac gatgctgcac cggcagatcc tcgagcggtt
     4141 cccgcagttc gacatcatcg tccggtacga ggcggatgaa atcctgcccg ccgtgctcga
     4201 ctgcctcccg caccggacat tcgacgtgat tcccggcttg agctggcgcg cgacgggccg
     4261 cggctcgccg ttgcgcttca ccgacggcaa gcccaaggtc gaggatctgg acctgctgcc
     4321 gatcgcctcg tacgaccatt atccggttga ggaactgggg ctctcgatgc tgcgcatcga
     4381 ggccggccga gggtgtccgt tcgcatgcac attctgctcg acggccggtt tctttcaacg
     4441 cagcttccgc ctcaaatcgg ccgagcgcct ggttcgcgaa ctggatatcc ttcaccagcg
     4501 ttatcgcgtg tcggacttca agctcgacca cgacatgttc accgtgaatc gccgcaaggt
     4561 aatggagttt tgcgaggcgg tcgccgggcg cgactatcgg tggcgcgcgt cggcgcggat
     4621 cgactgcgtc gacgaagcat tgttgaagaa gatggccgac gcgggctgcg tgaatcttta
     4681 tttcggcgtg gagacgggct ccgagcgcat gcaaaagctc tgcaagaagc ggctggatct
     4741 gcaacgggtc gagcccattc tggcggcggc ggattcattc ggaatcgaga cgaccgcgtc
     4801 gttcatcacc ggctatcccg aggaaaccgg ggaggatcag gacgacacgc tggacatgat
     4861 cgggcgctgc gctcggcgag cgtcttgcct cacgcaactg cacatgctcg cgccggagcc
     4921 cggcacaccg ctgttcgacg aacgcggcgc ggaaatcgcc tacgacggct gtggcgggcc
     4981 ttacaacacg cgcctgctga gttcgtcgga cgagcgtgcg gtgctcggcc atcccgacat
     5041 attccaaacc tactatcact accccgccgc gctgccgcgt gcgcgataca tctttgccgt
     5101 cggggcggcg gatctgctgc gccgggtcgg gccggtcatt ttcgcgtatg cgttgcgggg
     5161 gttcggaggg cggctgagca cgctgatatc ggcgtggcga caattcgccg acgaaacgtc
     5221 gcccggcgcg cccgcggacg cggcggggct cgaggccttc attgccgcgc ggttcggccg
     5281 cggccatcac ctgcattctc tttttcggta cgcgttgcgg ctctatgcgg cccgcgctcc
     5341 ggcggacgta atcgtcgagg ggccctatga gccggaccgg ccttgcatgc tggccgaaaa
     5401 cgtcggtctg ctggaggaca ttcacaattg cgacgcattg gtcgaccgga tcaggcgatc
     5461 gaccgaaggc gcgccgatgc tcggcgacgc ggatgtcggc gggctcggaa catatatcgt
     5521 tcaggggcac ggcgaggccg ccacgggcta ttgggtggag tccggcatcg ctacgcttct
     5581 cgatctgttc aggtcgcccc gttcgtgccg cgccgttgcg caatggctgg cggacgccac
     5641 ccgcaatgac ggcatagacg cttcgatctt cgagccgctg ctgcgcagag gcatcctggt
     5701 gtcggccggt taaatccatt tcgtccatgg tgcgtcgcac actcgaacgt tggtccgcgc
     5761 tggatctcgc ggaagggctt caactggcgc atgccgtcgc cgcgctgcag gcgctcggcg
     5821 ttctggacgc gatgagggag ccggtcacgg cggaaacgtt gagcgccagc catgatctcg
     5881 atcccgagtt gctgcgcggc atcctcgaat atgcggcgtc gagaacgaat ctggttcgca
     5941 agacggaccg cggattcgcg atttccgagc attacaccgc gcaagcgcgc tttctgttga
     6001 acctgtacgc cggcgcttat gccgataacg cttcacggct ggcgacgctg ctccggcgtc
     6061 cggcattggc gggctccatg atcgacctcg tcgcgcacgc gcgcgccttc gacgcgagcg
     6121 aggccggcgg tggagcgctg gcgtcgatcg tcacgcaatt gcgtctcaat catgtgctgg
     6181 atctgggatg cggcgccggc gcgctgctgc gcgagttggc ggcggacgat cgcggattcg
     6241 tcggctgggg actggatcgc aatcccgcga tgtgcaaggc ggcgcgggtt cgagcgcggc
     6301 aagcggggct cgccgcgcgc gtgaaggtgt tccacggcga cggctggaat cccggggcgt
     6361 cggtcccgcc gcgcgtgctg gcgcgcgtgc gcaacgtcgt cgcgagccag ttcgtcaacg
     6421 agatgtttcg cggcggcaca tcgcgagtgg aaacgtggct tcggcgcatg cggcgcttgc
     6481 tgcccggccg catgctcgtg atatcggact attacggccg cctcggccac ggcactcaca
     6541 cgatgcgccg cgagacgctg ctgcatgact atgtgcagct catatcgggg cagggcgtgc
     6601 cgccgcccga ttccgacgcg tggttggcca tgtatcgagc agccggttgc cggcctctgc
     6661 acgtgatcga agaccgggcg gccacgacat gcttcgtcca tctggtgggg ctatagtcta
     6721 gagtcgacct gcaggcatgc aagcttggct gttttggcgg atgagagaag attttcagcc
     6781 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
     6841 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     6901 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
     6961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     7021 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     7081 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
     7141 acggatggcc tttttgcgtt tctacaaact cttgttattg gtcgacttaa acgcctggtg
     7201 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac
     7261 ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc
     7321 ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt
     7381 gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc tgcctcccag
     7441 agcctgataa aaacggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg
     7501 tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcga
     7561 ggcggctaca gccgatagtc tggaacagcg cacttacggg ttgctgcgca acccaagtgc
     7621 taccggcgcg gcagcgtgac ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa
     7681 cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg
     7741 gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg gctggctggg cggctcctcg
     7801 ccggggccgg tcggtagttg ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg
     7861 ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac
     7921 accgacaggc gcaactggtc gcggggctgg ccccacgcca cgcggtcatt gaccacgtag
     7981 gccgacacgg tgccggggcc gttgagcttc acgacggaga tccagcgctc ggccaccaag
     8041 tccttgactg cgtattggac cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc
     8101 tggctgacca ccacggcgtt ctggtggccc atctgcgcca cgaggtgatg cagcagcatt
     8161 gccgccgtgg gtttcctcgc aataagcccg gcccacgcct catgcgcttt gcgttccgtt
     8221 tgcacccagt gaccgggctt gttcttggct tgaatgccga tttctctgga ctgcgtggcc
     8281 atgcttatct ccatgcggta ggggtgccgc acggttgcgg caccatgcgc aatcagctgc
     8341 aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc aattacagat
     8401 tttctttaac ctacgcaatg agctattgcg gggggtgccg caatgagctg ttgcgtaccc
     8461 ccctttttta agttgttgat ttttaagtct ttcgcatttc gccctatatc tagttctttg
     8521 gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg ctccccctcc
     8581 ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg cggccttcgg ccttgcccaa
     8641 ggtggcgctg cccccttgga acccccgcac tcgccgccgt gaggctcggg gggcaggcgg
     8701 gcgggcttcg cccttcgact gcccccactc gcataggctt gggtcgttcc aggcgcgtca
     8761 aggccaagcc gctgcgcggt cgctgcgcga gccttgaccc gccttccact tggtgtccaa
     8821 ccggcaagcg aagcgcgcag gccgcaggcc ggaggctttt ccccagagaa aattaaaaaa
     8881 attgatgggg caaggccgca ggccgcgcag ttggagccgg tgggtatgtg gtcgaaggct
     8941 gggtagccgg tgggcaatcc ctgtggtcaa gctcgtgggc aggcgcagcc tgtccatcag
     9001 cttgtccagc agggttgtcc acgggccgag cgaagcgagc cagccggtgg ccgctcgcgg
     9061 ccatcgtcca catatccacg ggctggcaag ggagcgcagc gaccgcgcag ggcgaagccc
     9121 ggagagcaag cccgtagggc gccgcagccg ccgtaggcgg tcacgacttt gcgaagcaaa
     9181 gtctagtgag tatactcaag cattgagtgg cccgccggag gcaccgcctt gcgctgcccc
     9241 cgtcgagccg gttggacacc aaaagggagg ggcaggcatg gcggcatacg cgatcatgcg
     9301 atgcaagaag ctggcgaaaa tgggcaacgt ggcggccagt ctcaagcacg cctaccgcga
     9361 gcgcgagacg cccaacgctg acgccagcag gacgccagag aacgagcact gggcggccag
     9421 cagcaccgat gaagcgatgg gccgactgcg cgagttgctg ccagagaagc ggcgcaagga
     9481 cgctgtgttg gcggtcgagt acgtcatgac ggccagcccg gaatggtgga agtcggccag
     9541 ccaagaacag caggcggcgt tcttcgagaa ggcgcacaag tggctggcgg acaagtacgg
     9601 ggcggatcgc atcgtgacgg ccagcatcca ccgtgacgaa accagcccgc acatgaccgc
     9661 gttcgtggtg ccgctgacgc aggacggcag gctgtcggcc aaggagttca tcggcaacaa
     9721 agcgcagatg acccgcgacc agaccacgtt tgcggccgct gtggccgatc tagggctgca
     9781 acggggcatc gagggcagca aggcacgtca cacgcgcatt caggcgttct acgaggccct
     9841 ggagcggcca ccagtgggcc acgtcaccat cagcccgcaa gcggtcgagc cacgcgccta
     9901 tgcaccgcag ggattggccg aaaagctggg aatctcaaag cgcgttgaga cgccggaagc
     9961 cgtggccgac cggctgacaa aagcggttcg gcaggggtat gagcctgccc tacaggccgc
    10021 cgcaggagcg cgtgagatgc gcaagaaggc cgatcaagcc caagagacgg cccgagacct
    10081 tcgggagcgc ctgaagcccg ttctggacgc cctggggccg ttgaatcggg atatgcaggc
    10141 caaggccgcc gcgatcatca aggccgtggg cgaaaagctg ctgacggaac agcgggaagt
    10201 ccagcgccag aaacaggccc agcgccagca ggaacgcggg cgcgcacatt tccccgaaaa
    10261 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    10321 atcacgaggc cctttcgtct tcgaataaat acctgtgacg gaagatcact tcgcagaata
    10381 aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg
    10441 agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc
    10501 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa
    10561 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
    10621 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
    10681 tttagctttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
    10741 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
    10801 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
    10861 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
    10921 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
    10981 agagcattac gctgacttga cgggacggcg gctttgttga ataaatcgaa cttttgctga
    11041 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa
    11101 gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg gctccctcac
    11161 tttctggctg gatgatgggg cgattcaggc ctggtatgag tcagcaacac cttcttcacg
    11221 aggcagacct cagcgccaga aggccgccag agaggccgag cgcggccgtg aggcttggac
    11281 gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga cgcggtggaa
    11341 agggggaggg gatgttgttt aaatggctct gctgtagtga gtggtctacg gggtctgacg
    11401 ctcagtggaa cgaaatcgat gataagctgt caaacatgag cagatcctct acgccggacg
    11461 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
    11521 caccgatggg agatcctaag atatcgctta ggccacacgt tcaagtgcag ccacaggata
    11581 aatttgcact gagcctgggt gggattcgga ctcgaccgca tagccttcag gagtgagttt
    11641 tgtgcaatac caaccgacga cttgaccctg ccaagcggca ccagatttct tgcgtacgcg
    11701 atcccctaag ccaaaggtgg cactcagggg aagcgcaaac tgccctgcaa cgggagcgtt
    11761 ggcttcatcg ctactttgac ccatgtcgaa tccttcttgt gaatctatta tggcgacaag
    11821 caaatcgagc tctgactgcc taccccacaa caactatcag aaagcaccag cacaacggct
    11881 gcctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat
    11941 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
    12001 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
    12061 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
    12121 cgaacaggct tatgtcaact gggttcgtgc gagctcatcg atgcatttaa tctttctgcg
    12181 aattgagatg acgccactgg ctgggcgtca tcccggtttc ccgggtaaac accaccgaaa
    12241 aatagttact atcttcaaag ccacattcgg tcgaaatatc actgattaac aggcggctat
    12301 gctggagaag atattgcgca tgacacactc tgacctgtcg cagatattga ttgatggtca
    12361 ttccagtctg ctggcgaaat tgctgacgca aaacgcgctc actgcacgat gcctcatcac
    12421 aaaatttatc cagcgcaaag ggacttttca ggctagccgc cagccgggta atcagcttat
    12481 ccagcaacgt ttcgctggat gttggcggca acgaatcact ggtgtaacga tggcgattca
    12541 gcaacatcac caactgcccg aacagcaact cagccatttc gttagcaaac ggcacatgct
    12601 gactactttc atgctcaagc tgaccgataa cctgccgcgc ctgcgccatc cccatgctac
    12661 ctaagcgcca gtgtggttgc cctgcgctgg cgttaaatcc cggaatcgcc ccctgccagt
    12721 caagattcag cttcagacgc tccgggcaat aaataatatt ctgcaaaacc agatcgttaa
    12781 cggaagcgta ggagtgttta tcgtcagcat gaatgtaaaa gagatcgcca cgggtaatgc
    12841 gataagggcg atcgttgagt acatgcaggc cattaccgcg ccagacaatc accagctcac
    12901 aaaaatcatg tgtatgttca gcaaagacat cttgcggata acggtcagcc acagcgactg
    12961 cctgctggtc gctggcaaaa aaatcatctt tgagaagttt taactgatgc gccaccgtgg
    13021 ctacctcggc cagagaacga agttgattat tcgcaatatg gcgtacaaat acgttgagaa
    13081 gattcgcgtt attgcagaaa gccatcccgt ccctggcgaa tatcacgcgg tgaccagtta
    13141 aactctcggc gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccaca gcgataggcg
    13201 atgtcagtaa cgctggcctc gctgtggcgt agcagatgtc gggctttcat cagtcgcagg
    13261 cggttcaggt atcgctgagg cgtcagtccc gtttgctgct taagctgccg atgtagcgta
    13321 cgcagtgaaa gagaaaattg atccgccacg gcatcccaat tcacctcatc ggcaaaatgg
    13381 tcctccagcc aggccagaag caagttgaga cgtgatgcgc tgttttccag gttctcctgc
    13441 aaactgcttt tacgcagcaa gagcagtaat tgcataaaca agatctcgcg actggcggtc
    13501 gagggtaaat cattttcccc ttcctgctgt tccatctgtg caaccagctg tcgcacctgc
    13561 tgcaatacgc tgtggttaac gcgccagtga gacggatact gcccatccag ctcttgtggc
    13621 agcaactgat tcagcccggc gagaaactga aatcgatccg gcgagcgata cagcacattg
    13681 gtcagacaca gattatcggt atgttcatac agatgccgat catgatcgcg tacgaaacag
    13741 accgtgccac cggtgatggt atagggctgc ccattaaaca catgaatacc cgtgccatgt
    13801 tcgacaatca caatttcatg aaaatcatga tgatgttcag gaaaatccgc ctgcgggagc
    13861 cggggttcta tcgccacgga cgcgttacca gacggaaaaa aatccacact atgtaatacg
    13921 gtcatactgg cctcctgatg tcgtcaacac ggcgaaatag taatcacgag gtcaggttct
    13981 taccttaaat tttcgacgga aaaccacgta aaaaacgtcg atttttcaag atacagcgtg
    14041 aattttcagg aaatgcggtg agcatcacat
//
