LOCUS       sequence-450967-       10013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter.
            Has a Blasticidin resistance gene for stable cell line generation 
            and Ampicilin for plasmid propagation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Ross C, Gong L-Y, Jenkins LM, Ha N-H, Majocha M, Hunter K
  TITLE     SMARCD1 is an essential expression-restricted metastasis modifier
  JOURNAL   bioRxiv 2024.01.24.577061
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.24.577061"
COMMENT     Sequence Label: pDest-pol2-Smarcd1-Myc
FEATURES             Location/Qualifiers
     source          1..10013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(15..26)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(183..203)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        188..517
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      368..503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        565..612
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             631..1029
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1116..1349
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1421..1542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1458..1477)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1512..1531
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1582..1717
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1737..1756)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1738..1756)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1766..1783)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1766..1782)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1775..1797)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1924..2379
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2011..2030)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2221..2242
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2405..2509
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2510..2578,2579..3370)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2728..2747)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3541..4129
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4030..4049
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4283..4300
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4417..4438
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4453..4458,4459..4476,4477..4483)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4491..4507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4496..4518
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4515..4531
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4515..4531
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4550..4570
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4552..4570
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4598..4824
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4825..5005
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5052..5177
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5670..5903
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6088..6132
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6399..6515
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6526..6546
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7259..7283
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      7302..7311
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8865..8889)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8891..8920
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    8922..9510
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9393..9404)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(9518..9538)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    join(9557..10013,1..132)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
ORIGIN
        1 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
       61 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
      121 ccgcctcccc gcctggaatt ctgcagatat ccggttagta atgagtttgg aattaattct
      181 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      241 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
      301 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
      361 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
      421 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
      481 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
      541 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
      601 gtataatacg acaaggtgag gaactaaacc atggccaagc ctttgtctca agaagaatcc
      661 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
      721 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
      781 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
      841 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
      901 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
      961 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
     1021 gagggctaag cacaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
     1081 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg
     1141 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     1201 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     1261 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1321 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     1381 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     1441 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     1501 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     1561 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     1621 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     1681 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     1741 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     1801 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     1861 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     1921 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     1981 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     2041 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2101 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     2161 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2221 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     2281 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2341 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     2401 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2461 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2521 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2581 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2641 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2701 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2761 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2821 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2881 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     2941 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3001 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3061 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3121 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3181 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3241 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3301 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3361 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3421 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3481 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3541 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3601 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3661 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3721 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3781 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3841 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3901 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3961 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4021 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4081 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4141 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4201 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4261 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     4321 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     4381 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     4441 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     4501 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     4561 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     4621 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     4681 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     4741 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     4801 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     4861 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4921 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4981 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     5041 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     5101 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     5161 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     5221 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     5281 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     5341 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     5401 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     5461 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     5521 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     5581 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     5641 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     5701 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     5761 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     5821 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     5881 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     5941 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     6001 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     6061 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     6121 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     6181 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     6241 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     6301 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     6361 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     6421 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     6481 gaattacaaa aacaaattac aaaattcaaa attttatcgt ccaatcaact ttgtatagaa
     6541 aagttggcaa gcttgggctt gaacatcgag cgccagggct ccgtaaagct actagagcac
     6601 aggcggtgcc ccaacgtcct ggggcctctc cactaataac ggctacttcc aattgattgg
     6661 acgcgccatc ttgcctgcct tatgcatatt cagcggtgaa ctgaatattc atgaacgagg
     6721 cccgtcccgt ccctccctcc ttccccccac ccccggaacc cgctccggag gacccgaagg
     6781 gccccgcctt cattaccgat gcgtaggaca aaccattttc ccgatgtgtg tggggggata
     6841 ctaatgagag actttagctg aaaatgagcc tgaactccga agctgagtaa aaatggccta
     6901 actttatcct ccgttctgta agtcctcggt ttgagtgcac gggaaacccg aaaggaggac
     6961 gacaggacca ggaacattct cctcctcctg tcgcgtcaga aagaacaccc aaccagggag
     7021 ccggagccct agcgtcaaca actccgccgc gcgcgctccg tgtaggccgg tgcgggcggc
     7081 cccgtagcgc aagggagggc gggaaaggaa ggggcgggac acaagggcga atctataaag
     7141 ggcgtcactc agccagttct ctcctcagaa gcgccgagag cgcgaccggg acggttggag
     7201 aagaaggtgg ctcccggaag ggggagagac aaactgccgt aacctctgcc gttcaggatc
     7261 aactttgtac aaaaaagcag gctccgcggc cgcccccttc accatggcgg cccgggcggg
     7321 tttccagtct gtggctccga gcggcggcgc gggagcctca ggaggagcgg gcgtggcggc
     7381 tgctctgggc ccgggcggaa ctcccgggcc tcccgtgcga atgggcccgg cgccgggtca
     7441 agggctgtac cgctctccga tgcccggggc ggcctatccg agaccaggta tgctgccagg
     7501 tagccgaatg acacctcagg gaccttccat gggacctcct ggctatgggg ggaacccttc
     7561 agtccgacct ggtctggccc agtcagggat ggaccagtcc cgcaagagac ctgcacctca
     7621 acagatccag caggtccagc agcaggcggt ccaaaatcga aatcacaatg caaagaaaaa
     7681 gaagatggct gacaaaatcc tacctcaaag gattcgggaa ctggtcccag aatcacaggc
     7741 ctacatggat ctcctggctt ttgaaaggaa actggaccag actattatga ggaagcggct
     7801 agatatccag gaggccttga aacgtcccat caagcaaaaa cggaagctgc gaattttcat
     7861 ttctaacacg ttcaatccgg ctaagtcgga cgcggaggat ggggaaggga cggtggcttc
     7921 ctgggagctc cgggtagaag gccggctcct ggaggacgcg gccttgtcca aatatgacgc
     7981 caccaagcaa aagagaaagt tctcttcctt ttttaagtcc ttggtgatcg aactggacaa
     8041 agacctctat ggcccagaca accatctggt agaatggcac aggaccgcca ctacccagga
     8101 gaccgatggc ttccaggtga agcggccagg agatgtgaat gtacggtgta ctgtcctgct
     8161 gatgctggac taccagcccc cccagtttaa attagaccct cgcctggctc ggctcttggg
     8221 catccatacc cagacacgtc cagtgatcat ccaagcactg tggcagtata ttaaaacaca
     8281 caagctccag gaccctcacg agcgagagtt tgttctctgt gacaagtacc tccagcagat
     8341 ctttgaatct cagcggatga agttctcaga gatccctcag cggctccacg ccttgcttat
     8401 gccaccagag cccatcatca tcaatcatgt catcagtgtg gacccaaatg accagaaaaa
     8461 gaccgcgtgc tatgacattg acgtggaggt ggatgacact ctgaagaccc agatgaactc
     8521 tttcctgttg tccactgcca gccagcagga gatcgccact ctagacaaca agatccatga
     8581 gacgatagag accatcaacc agctgaagac ccagcgagag ttcatgttga gctttgcccg
     8641 agaccctcag ggtttcatca atgattggct tcagtcccag tgcagggacc tcaagacgat
     8701 gactgatgtg gtgggtaacc cggaagagga gcgtcgtgct gagttctact tccagccctg
     8761 ggctcaggag gctgtgtgcc gatacttcta ctccaaggtg cagcagaggc ggcaagagtt
     8821 agagcaagcc ctgggaatcc gaaacacaaa gggtgggcgc gccgacccag ctttcttgta
     8881 caaagtgggc gaacaaaagc tcatatctga agaggaccta taatcaacct ctggattaca
     8941 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     9001 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     9061 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     9121 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     9181 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     9241 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     9301 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     9361 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     9421 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     9481 gtcggatctc cctttgggcc gcctccccgc ctgtaatcaa ctttattata catagttgat
     9541 gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     9601 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     9661 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     9721 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     9781 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     9841 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     9901 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     9961 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt cct
//
