LOCUS       sequence-451138-        3204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For gateway cloning of NUDT5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Weixler L, Zaja R, Ikenga NJ, Mohan G, Aydin G, Wijngaarden S, 
            Filippov DV, Luscher B, Feijs-Zaja KLH
  TITLE     Family-wide analysis of human macrodomains reveals novel activities
  JOURNAL   ResearchSquare. 2024 Aug 8. doi: 10.21203/rs.3.rs-4644671/v1
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "ResearchSquare. 2024 Aug 8. doi: 10.21203/rs.3.rs-4644671/v1"
COMMENT     Sequence Label: pDONR221-NUDT5
FEATURES             Location/Qualifiers
     source          1..3204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1329..1428)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1445..1464)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1446..1464)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1469..1485)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1469..1485)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1587..1606)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1598..2407
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1665..1684)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2554..3142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3043..3062
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gagagccaag agccaaccga aagctcccag aacgggaaac agtacattat
      721 ttcggaagag ttaatcagcg aaggtaagtg ggtcaaactt gaaaagacca cctacatgga
      781 tcctactggc aagacacgta catgggaatc tgttaaacgt actacacgta aggaacagac
      841 cgctgatgga gttgccgtaa ttccagttct tcagcgtact cttcactacg aatgcatcgt
      901 tctggtaaag cagtttcgcc caccgatggg aggttattgt attgagtttc ccgctggtct
      961 tatcgatgac ggtgagacac cagaggctgc ggcattgcgt gagcttgagg aagaaaccgg
     1021 ttataagggc gacatcgcgg agtgttcacc agctgtttgt atggacccgg gtttatcaaa
     1081 ctgtactatt cacattgtaa ctgtcacgat caatggtgac gatgcagaga acgctcgtcc
     1141 aaaaccgaaa cctggcgatg gcgagtttgt agaggtaatc tccttgccaa agaacgacct
     1201 tttacagcgc ttggacgccc ttgttgctga ggaacatttg acagttgatg cgcgtgttta
     1261 ctcttacgcc ttggcgctta aacacgccaa cgctaagccg tttgaagtac ccttcttaaa
     1321 gttttaatac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     1381 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     1441 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     1501 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     1561 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     1621 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     1681 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
     1741 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     1801 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     1861 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
     1921 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     1981 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     2041 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     2101 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
     2161 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     2221 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     2281 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     2341 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     2401 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
     2461 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     2521 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2581 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2641 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2701 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2761 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2821 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2881 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2941 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3001 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3061 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3121 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3181 ttgctggcct tttgctcaca tgtt
//
