LOCUS       sequence-451367-       10820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. acnes with tetR-based inducible expression of mCherry. pBRESP36A 
            with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Nevot G, Santos-Moreno J, Campama-Sanz N, Toloza L, Parra-Cid C, 
            Jansen PAM, Barbier I, Ledesma-Amaro R, van den Bogaard EH, Guell M
  TITLE     Synthetically programmed antioxidant delivery by a domesticated skin
            commensal.
  JOURNAL   Cell Syst. 2025 Feb 19;16(2):101169. doi: 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6.
  PUBMED    39919749
REFERENCE   2  (bases 1 to 10820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-02-19"; volume: "16(2):101169. doi"; pages: " 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6"
COMMENT     Sequence Label: pEND-303_Cacnes_tetR
FEATURES             Location/Qualifiers
     source          1..10820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3914..4105
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(4099..4121)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4207..4347
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4262..4281
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4362..4379)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4533..5121)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4613..4632)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5292..6083,6084..6152))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5915..5934
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6153..6257)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6325..6343
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6408..6426
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     misc_feature    7794..7812
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     RBS             8309..8331
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             8960..8977
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             10190..10201
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             10202..10243
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             10253..10270
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgcccggtca tgacgctgag ttagtggcgc agctgcgctc tgagaacgag tttttacggc
       61 gtcaggtcga gcagcaggcg cgcacgatcg aacggcaggc tgaggcacac gcggtggtct
      121 cagcgcagct cacacgggtt ggccagcttg aggccggcga cgcagcagca ccgacactgg
      181 cacccgttga aaggccggct ccgcgacggc ggtggtggca gcgtcggtag cggtcaggat
      241 cgctctggcg tgacgagtgt gtctggcagt gcgaacagtt gctcgaccag tggcagcaga
      301 agcgagatcg ctgcgtggtg ctgttcctcg gtcagttcgt cgaggactgg cgggtcttgc
      361 tgcgtccagc cgatcgcctc ggcggccaag gtcagttcca agctgtgcca acgcacacgc
      421 ccctcggctg acagctgagt ctcgaactgt gcaactggac cggccggaag atgcacgttg
      481 ccgaggtcgt gagtggccaa gcgcacgtca aagagtgctg cttcgtagcc gcgcagaaat
      541 ggcagtgctc ggtcgattcg gatcggcctg cccaggtaca ttccgggccg cttgatgaac
      601 gcctccgcgt agaagcgcac cgttctcggc ccggcctcgt gatctgtcac tgtgcacgct
      661 cctctcgatg gttctcgacg ctaccggaga ccaccgacgt tcatgcccag cgcagcgacc
      721 tgaaaggacc aagccgagtt agccgtgcta accgtatagc ttgctccgtc gcctctgagg
      781 gcaaccacct gcgcagcagg tgggcggcag cccgcgcgca agcgcctacc gggtttgggc
      841 acagcccata aatcaacgcc tccggtgttg aagcgatcgt gtgtcacgat tgctatgctt
      901 gctacccctt cagggttttc gtatacacaa atcaagtttt ttcgtatacg ctaatgccat
      961 gagtgagcat ctactgcacg gcaagcccgt caccaacgag cagattcagg catgggcaga
     1021 cgaggccgag gccggatacg acctgcccaa actccccaag ccacggcgcg gacgcccgcc
     1081 cgtaggagac ggtccgggca ccgtcgtacc cgtgcgtctc gacgcggcca ccgttgccgc
     1141 tctcacagaa cgagcaacag ccgagggcat cacgaaccgt tcagacgcga tccgagccgc
     1201 agtccacgag tggacacggg ttgcctgacc tccacgactc agcacgcaag cactaccaac
     1261 gagaccggct cgacgacacg gccgtgctct acgcggccac ccacgttctc aactcccggc
     1321 cactcgacga cgaagacgac ccgcgccgct ggctcatgat cggaaccgac ccagcaggcc
     1381 gcctactcga actcgtcgca ctgatctacg acgacggcta cgaactgatc atccacgcaa
     1441 tgaaagcccg cacccaatac ctcgaccagc tctaaccaag aaaggaacct gatgagcgac
     1501 cagctagaca gcgaccgcaa ctacgacccg atgatcttcg acgtgatgcg cgagaccgcg
     1561 aaccgcgtcg tcgccacgta cgttgcatgg gaagatgaag ccgctgatcc ccgcgaggct
     1621 gcgcactggc aggccgagcg attccgcacc cggcacgagg tgcgcgccgt cgaccctgac
     1681 agccggcgag cagttcaggc gaagatcgca cagctgcgcg aggaactagc cgcaatgccc
     1741 gaacacgccc cagccatccc ttggagcagg tggcagcgtc aggggagtcg ggggatgttt
     1801 ggcaggggat gtggaaagag agttcgcttt gctcacatgg ctcaaccggg taactaactg
     1861 atatggggtc ttcgtcgccc actttgaaca cgccgaggaa tggaccacgc tgaacgtgac
     1921 tcgcatgctt cactgcatgt atggattcgt tcgagacgtt gttccctgag agctggctgc
     1981 cacgcaagcc gctggcgtca gccgagaagt ctggggcgta ccggcacgtg actcggcaga
     2041 gggcgctgga gctgccttac atcgaagcga acccgttggt catgcagtcc ttggtcatca
     2101 ccgatcgaga tgcttcggat gctgactggg ccgcagacct cgctgggctg ccttcaccgt
     2161 cctacgtgtc catgaaccgt gtcacgacca ccggacacat cgtctatgcc ttgaagaacc
     2221 ctgtgtgtct gaccgatgcc gcgcggcgac ggcctatcaa cctgctcgcc cgcgtcgagc
     2281 agggcctatg cgacgttctc ggcggcgatg catcctacgg gcaccggatc acaaagaacc
     2341 cgctcagcac cgcccatgcg accctctggg gccccgcaga cgcgctctac gagctgcgcg
     2401 ccctcgcaca caccctcgac gagatccacg cactgccgga ggcagggaac ccgcgtcgca
     2461 acgtcacccg atcaacggtc ggccgcaacg tcaccctgtt cgacaccacc cgcatgtggg
     2521 cataccgggc cgtccggcac tcctggggcg gcccggtcgc cgaatgggag cacaccgtat
     2581 tcgagcacat ccacctactg aacgagacga tcatcgccga cgaattcgcc acaggccccc
     2641 tcggcttgaa cgaacttaag cacttatctc gatccatttc ccgatgggtc tggcgcaact
     2701 tcacccccga aaccttccgc gcacgccaga aagcgatcag cctccgtgga gcatccaaag
     2761 gcggcaaaga aggcggccac aaaggcggca ttgccagtgg cgcatcacgg cgcgcccata
     2821 cccgtcaaca gttcttggag ggtctctcat gaccacacgt gaacgtctcc cccgcaacgg
     2881 ctacagcatc gccgctgctg cgaaaaagct cggtgtctcc gagtccaccg tcaagcggtg
     2941 gacttccgag ccacgcgagg agttcgtggc ccgcgttgcc gcacgccacg cgcggattcg
     3001 tgagctgcgc tcggagggtc agagcatgcg tgcgattgct gccgaggtcg gggtttccgt
     3061 gggcaccgtg cactacgcgc tgaacaagaa tcgaactgac gcatgaccgt aacgccgcac
     3121 gatgagcatt ttcttgatcg tgcaccgctt ggcactacgt tcgcgtgcgg ttgcacagtg
     3181 cgcgccacgt tcttatcctg cggccattgt ggctacagcc aatggggggc atcagcaacg
     3241 gacgttgaac ccggtgggca agtgttactc agggggacat gcccagtctg cggcgctcgg
     3301 attgacggta tggcagtcgt gcatgcggcc ccaccgtcaa actcattcag gtatcagtga
     3361 gaaccctcat ggcaccccct cgtgacacgt tctcgttgcg atcagctgcg gccaagcttg
     3421 atcttcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag
     3481 gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga
     3541 gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt
     3601 cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat
     3661 gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt
     3721 aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc
     3781 cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat
     3841 cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta
     3901 cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag
     3961 aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga
     4021 catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt
     4081 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     4141 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     4201 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
     4261 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     4321 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
     4381 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4441 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4501 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4561 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4621 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4681 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4741 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4801 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4861 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4921 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     4981 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5041 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5101 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5161 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5221 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5281 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5341 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5401 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5461 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5521 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5581 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     5641 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5701 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5761 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5821 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5881 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     5941 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6001 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6061 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6121 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6181 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6241 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     6301 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
     6361 ttcaagcttg tcgacgttaa cctgcaggca tgcggatccg gtaccagctg tctcttatac
     6421 acatctctgc agttaacgct gacccggacc accacgacga cccggacgac caccggtacg
     6481 accaccacca gacggaccgc ggcgaccacc ctggtcaccc tgttcacgtt caccggtacg
     6541 accgtcgtca cggttacgac cagaagaacg acggtcaccg tgaccagacg cttgccggtc
     6601 tctgcgatct tggcccccgg tacgtctgtc accacggtcc tgaccaccgg tacgacggtc
     6661 gcccctatcc tgtccacccg tcctacgacc acgctgcggc tgaccaccac gcggttcgtt
     6721 tttctggtcc agacgttcga acagacgcag ccactgttcc ggacgaacgt aagcaacaac
     6781 agcaccacca ccaacaccag cgtggtccag agcagcttca acacgacgac gcgggtattt
     6841 acgcagcaga gaagcctgga tgttaccacc aacaccggtg aagcacagtt caaccataga
     6901 ttcgtaacgt tccagagcag caccttccag cagcggttca gcacgacgac gcagacgcat
     6961 gatagcagag tcaactttcg gaaccggttt gaacagacga cgatcgactt tttcaacgaa
     7021 ttcccattcg aacagcggcc aggtcataac ggtcagacga gaccaacgac cgtagtcacc
     7081 ggtacgttta cgagcgaatt ccagctgggt aaccatggta gcggtttcga tggtcggagc
     7141 ttccaggcac cagtcaacga tagcagaggt gataccgtac gggatagcac caacgaaagc
     7201 gaacggttcc ggcggcggtt cagcggtcag gaagtcagcg ttaacaactt cgatgttcgg
     7261 gtgaccagac agtttttcac gcagagattt agccagacgc gggtcgattt cgtaagaggt
     7321 aacctgacga gcacggtcag ccagttcacg ggtcagcaga ccttcacccg gaccagcttc
     7381 cagaaccggc aggtccggac gcagttcagc ggtttcagcg atacgagcga tggttttacg
     7441 gtcacgcagg aagttctggc cgaattggcg gcggttacgg tcacgctcgg tacggccgag
     7501 gacggggcga ttctggttgg ggtgctggcg gtcttggttg cggcggcggg ggcggggctg
     7561 ttcatcggag gaggacatga cgatctcccg atgtgtcgta cgtatgggaa ttgacgaaaa
     7621 tcatatagat tttcagttga agtgtagcac tgcatagtgt catcgaagtg tcggcggcaa
     7681 cggtatgccc ggtagggggt gggtctgcct ttcggtttcg tgaacctgat gcggcgacgc
     7741 gcatgtagcc gataacccct agactcgccc gtgcagatcg tcggtgtgga tccagatgtg
     7801 tataagagac agctggtacc ggccgctgct gtgcgtgcgg gcgtgagcgt ttctacgctg
     7861 cggcgcagga aatcagagct tgaggctgcc ggagcgacgg tagacccgtc cggttgggtg
     7921 gtgccactgc gtgcactcaa ggtcgttttt ggggtgtcag atgagacctc gaatgcgaat
     7981 accagtaaat cctgacctgt gagttgtgaa ttcataggat agattctgga aactttaccg
     8041 tccgagctcc agcctgcggt ccggacaccg acgatctgca cgggcgagtc taggggttat
     8101 cggctacatg cgcgtcgccg catcaggttc acgaaaccga aaggcagacc caccccctac
     8161 cgggcatacc gttgccgccg acacttcgat gacactatgc agtgctacac ttcaactgaa
     8221 aatctatatg attttcgtca attctcgctg ggacgcccgt agaactctgt gaggataaag
     8281 tctcccttac agcctgcggt ccggtaattt tgtttaactt taagaaggag atatacatat
     8341 gtcaagacta gataagagca aggtcattaa cagcgcactg gagttactaa acgaagttgg
     8401 aatagaggga ttgactacac gaaaattagc acagaaatta ggagtagaac aaccgacact
     8461 ttattggcac gttaaaaata aaagagcttt gctggatgct ttagctatag aaatgctaga
     8521 ccgacaccac acacattttt gccccttaga aggagaaagt tggcaagatt tcctacgtaa
     8581 caacgccaaa tcatttagat gcgcgctatt atcacaccgc gacggcgcca aagttcacct
     8641 cgggacaaga cctacggaga agcaatacga aaccctagag aatcaattag cctttctatg
     8701 tcaacaagga ttcagtttgg aaaacgccct ctacgcccta tcagccgttg gtcattttac
     8761 attgggatgc gttttagagg atcaggaaca tcaagtagca aaagaagaaa gagagacacc
     8821 caccaccgac agcatgccac cattattacg tcaagctatt gaactattcg atcatcaggg
     8881 agcggaaccg gcatttttat ttggcctcga attaatcata tgtggtttag aaaaacaatt
     8941 aaagtgtgaa tctggatccc atcatcacca tcaccactga taatcgctgg gacgcccgct
     9001 cgagcaataa acagttgata gggcttctcc gttaccatgg ttcagccaaa aaacttaaga
     9061 ccgccggtct tgtccactac cttgcagtaa tgcggtggac aggatcggcg gttttctttt
     9121 ctcttctcaa ttcttctgac ctgtaacgaa taatagatag taaagtagtc tccgattgag
     9181 ttttctctgc cgagtcccac ccagttctgt gatttcagta agttggtaat tgatacactg
     9241 ttgcgagaac tggtgcctgg tagtagatag gttgttattg agtaagaagg taaagtgaac
     9301 gaaatccctg aaactgagac tgtagaaaat aagcttcagc ctgcggtccg gttgacaccc
     9361 tatcagtgat agagtataat gctagctcgc tgggacgccc ggtagtgctt atcagaccca
     9421 atactgttga acagcctgcg gtccggtcca cttataaata tagaggaggt ttgatatggt
     9481 gtccaaaggc gaggaggata acatggcgat catcaaggag ttcatgcgct tcaaggtcca
     9541 catggagggc tccgtcaacg gccacgaatt tgagatcgaa ggtgaggggg agggccggcc
     9601 gtacgaaggc acgcagacgg ccaagctgaa ggtcaccaaa gggggcccgc tgcccttcgc
     9661 gtgggacatc ctgtcccctc agttcatgta cggttccaag gcctacgtca agcaccccgc
     9721 ggacattccg gactacctga aactctcctt cccggaaggc ttcaaatggg agcgggtcat
     9781 gaacttcgaa gacggcggtg tggtcaccgt cacccaggac tcgtcccttc aagacggtga
     9841 gtttatctac aaggtcaagc tgcgcggtac caactttccg tccgacggcc ctgtcatgca
     9901 gaagaagact atgggctggg aggccagctc cgagcgtatg tatccagagg atggcgctct
     9961 taagggtgag atcaagcaga gactgaagct caaggacggc ggtcactacg acgccgaggt
    10021 caagaccacc tacaaggcga agaaaccggt ccagctgccg ggcgcttaca acgtcaacat
    10081 caagctggac atcacttctc acaacgagga ctacactatc gtcgagcaat atgagcgtgc
    10141 cgagggccgc cactccactg gcggcatgga cgagctgtac aagtcccgca tcgagggccg
    10201 cggtaagccg atcccgaacc cgctgttggg cctggattcc actcgcacgg gccaccatca
    10261 ccaccaccac tgatcgctgg gacgcccggg atccaagaga tttctacacg attgagcact
    10321 gtctcctgca ggctcggtac caaattccag aaaagaggcc tcccgaaagg ggggcctttt
    10381 ttcgttttgg tcctaataga taaaggatag gtctggtagt gttgttcgtt ctcgcaggta
    10441 aatcaataat actctgcagt tccgtagact tttcagtggg acagggtagc gataacagat
    10501 agattgtaat aagacacagt aggtgctcgt agttgcgtga agagaaccgc tcaggaaatc
    10561 cagtcagaag tattggtaat cgttgaaaac tcagtcgacg cacttactga agacgtccta
    10621 ttacactcgt cgttggaaac tgaagatgcg gccgcggaaa cacagaaaaa agcccgcacc
    10681 tgacagtgcg ggcttttttt ttcgaccaaa ggtagcgaac gacgagtcac tgttgaggat
    10741 aaatactttc tctactaggc gcctgttaca caggtcctca gcggcgcgcc gaaactgaac
    10801 ctgttatttt acgctacgga
//
