LOCUS       sequence-451378-        4523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suicide C. acnes vector to generate LysA (PPA_RS06345) deletion 
            leaving an Ery resistance cassette flanked by BxbI recombination 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Nevot G, Santos-Moreno J, Campama-Sanz N, Toloza L, Parra-Cid C, 
            Jansen PAM, Barbier I, Ledesma-Amaro R, van den Bogaard EH, Guell M
  TITLE     Synthetically programmed antioxidant delivery by a domesticated skin
            commensal.
  JOURNAL   Cell Syst. 2025 Feb 19;16(2):101169. doi: 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6.
  PUBMED    39919749
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-02-19"; volume: "16(2):101169. doi"; pages: " 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6"
COMMENT     Sequence Label: pEND-418_Cacnes_LysA
FEATURES             Location/Qualifiers
     source          1..4523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1065..1122
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attP site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     rep_origin      complement(1776..2364)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1856..1875)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2447..3238,3239..3307))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3308..3412)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    4062..4110
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 aacggccgaa ctgcgccccg accttccggt cctggaggct ggcccaggcg agggtctgct
       61 gacccgtgag cttgccgatc gggcccgcca ggtgacgagc tacgagatcg acccgcgctt
      121 ggcgaagtcc ctgcgtgaga agctgtccgg ccacccgaat atcgaggtcg tcaacgcgga
      181 cttcctgacc gccgagcccc ctcccgagcc gtttgccttc gtcggcgcga tcccgtacgg
      241 catcacgtct gcgatcgtcg attggtgcct ggaggctccg acgatcgaaa ccgccacgat
      301 ggttacccaa ctcgaatttg cccgtaagcg caccggtgat tatggccgct ggtcccgcct
      361 cacggtcatg acttggccgc tcttcgagtg ggagttcgtc gagaaggtcg atcgccgctt
      421 gttcaagccg gtcccgaagg tcgattccgc catcatgcgt ctgcgtcgcc gcgctgaacc
      481 gctgttggag ggtgctgccc ttgagcgcta cgagagcatg gtggagttgt gttttactgg
      541 tgtcggcggc aacattcaag cctccctcct gcgcaagtac cctcgtcgtc gcgtggaggc
      601 tgcgctggac catgccggcg tgggtggtgg tgctgtcgtg gcctacgtcc ggccggagca
      661 atggctgcgg ctcttcgagc gccttgacca gaagaacgag ccgcgcggtg gccaaccgca
      721 gcgcggccgt cgtacgggtg gtcaggatcg tggtgaccgc cgtacgggtg gccaggatcg
      781 tggcgaccgt cggacgggtg gtcaggaccg tcgcgaccgt caggcctccg gccacggtga
      841 ccgtcggtcc tctggccgta atcgcgacga tggtcgtacc ggggagcgcg agcagggcga
      901 ccagggtggt cgtcgtggcc cttcgggtgg tggtcgtacc ggcggtcgtc caggccgtcg
      961 tggcggtccg ggtcagcgct gatctagacc cgcacttaac ccgcttcggc gggtttttgt
     1021 tttttggggt gcaggtaggc catattgtct tcggggcccc actcgtcgtg gtttgtctgg
     1081 tcaaccaccg cggtctcagt ggtgtacggt acaaaccccg acgaggcgat aagctcggca
     1141 aggctgatta cgcctgctcg tggaattcta gaactctgtg aggataaagt ctcccttaca
     1201 gcctgcggtc cggcgcatcg aaaagtccta ttacgcacta tctgtgaact ttcatcagct
     1261 gacaacaacg aggtgaggaa gagatgacga ataccaccga ttccgcgtca gcccagccga
     1321 tacgcgtggc cttgctgggg tgcggggttg tggggtccca ggtggcccgg atgattgaat
     1381 cgaggacgga ggatatcgcc gctcgagtgg ggcggcaggt cgagctcgct ggtattgctg
     1441 tcgcccatcc tgatcgcccg cgccagggcc ttgactcgcg tctttttacc gatgatccca
     1501 cagtgctcgt taaccgggac gatgtggata tcgtcatcga gctcatcggt ggcatcgacc
     1561 ctgcccgtca gctcctgaca gctgccctcg gcaaggggca ctccgttgtg acggcgaata
     1621 aggccctgtt ggcagctcac gggactgagt tacacgctgt ggccgagagc aacggcgtcg
     1681 atctgtacta cgaagctgcg gtagccgggg caatcgctgg gacgcccgct aggctggcgg
     1741 ggttgcctta ctgatcaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1801 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1861 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1921 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1981 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2041 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2101 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2161 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     2221 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2281 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2341 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2401 cagtggaacg aaaactcacg ttaagggatt ttggtcatga ggcgcctcac caatgcttga
     2461 tgagggaggc accgatctcg gcgatctgac ggttgcgctc atccatcgtg gcctgggagc
     2521 cggtcgtgta gatcaccacg atgcggctgg gcttaccgtc gggaccgaga gccgcgatga
     2581 tgccacgcga gccgcgctca ccagcacccg acttgtccgc gatgaaccaa ccggccggga
     2641 gcgcgctgcg caacagcggg ccagcgacct tgtcggcctc catccagtcg atcagttgct
     2701 gacgggaggc cagagtgagg agttcaccgg tcaggagttt acggagggtg gtagccatag
     2761 ccgcgggcat agtcgtgtcg cgctcgtcgt tcgggatagc ctcattaagt tcgggttccc
     2821 aacggtccag acgggtcacg tggtcgccca tgttgtggag aaaagccgtc agctctttcg
     2881 ggccaccgat ggtcgtcaac agcagattgg cggcagtgtt gtcggacata gtgatggccg
     2941 cggagcacag ctcgcgcacg gtcatcccat cggtcagatg tttctccgtg accggggagt
     3001 attcgaccag gtcgttctgg gagtaatgaa tgcgacggcc cagttgctcc tgcccagcat
     3061 ccacccggct aagcacagcg ccgcagagga ggaccttgaa cgtggacatc atggggaaac
     3121 gctcctcagg gcggaaagac tccagaatct taccggagtt caggtccaac tcgatataac
     3181 cgacgcgcgc ccccagctga tcctcggcgt ccttgacctt gaccagggtt tccggatggg
     3241 caaaaaccgg caagcaaaag gccgcaaaaa atgggataag ggcaacgcgg aagtgctgga
     3301 tcgacatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3361 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgactagttc
     3421 cccgaaaagt gccacctgac gttaagaaac cattattaca gcctgcggtc cggcggccca
     3481 atagagggtt cgcatgaacc acacaacagg cagcgggtat tccggcagga cggacgaatc
     3541 tggttgtccg ctcgacatcc accacaggta gtaccggagg tcacccgtga tgtagttcga
     3601 tgacggctgg ataaagacgt gaagcatgac gagtcgaccc actgtccaca gcacgatggc
     3661 acctgccgca gaaaacagcg cagctcggga tccttggcga gcggttttgg cgtcacgaaa
     3721 tggcacgaac tgacacccta cctgataaat ccaggtacgc cgctaaggac atagctcgag
     3781 cgctcccgga gggattcgaa ccctcgctcc cgcctccgga gggcggtgct ctatcccctg
     3841 agctacggga gccgacaggc ccgaaggccc cgaccacctg tggagtttag cgcaccggtg
     3901 ggctgctcgc cattcaagat cgcgagcatg gacactctgc gatcgtgtcg gtggcctctg
     3961 gcaaggtgta acctcgctgg gacgcccgta gaactctgtg aggataaagt ctcccttaaa
     4021 gcttgcgcca accatgcacc atagccagcc tgacagtagc ccggccggct tgtcgacgac
     4081 ggcggtctcc gtcgtcagga tcatccgggc gcagctgaga gcgacacagt cctgtacttg
     4141 ggatgctggc acaccgacga tctgcacggg cgagtctagg ggttatcggc tacatgcgcg
     4201 tcgccgcatc aggttcacga aaccgaaagg cagacccacc ccctaccggg cataccgttg
     4261 ccgccgacac ttcgatgaca ctatgcagtg ctacacttca actgaaaatc tatatgattt
     4321 tcgtcaattc ccatacgtac gacacatcgg gagatcgtca tgtcgtcgtc cgacgagcaa
     4381 ccgcgcccgc gtcgccgcaa ccaggaccgc cagcacccga accagaaccg cccggtcctg
     4441 ggccgtactg agcgggaccg caaccgccgt caattcggtc agaatttcct tcgtgaccgt
     4501 aagacgatcg cccgcatcgc gga
//
