LOCUS       sequence-451444-        7330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase reporter for in vivo bioluminescence imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Lee YH, Andersen JB, Song HT, Judge AD, Seo D, Ishikawa T, Marquardt
            JU, Kitade M, Durkin ME, Raggi C, Woo HG, Conner EA, Avital I, 
            Maclachlan I, Factor VM, Thorgeirsson SS
  TITLE     Definition of ubiquitination modulator COP1 as a novel therapeutic 
            target in human hepatocellular carcinoma.
  JOURNAL   Cancer Res. 2010 Nov 1;70(21):8264-9. doi: 
            10.1158/0008-5472.CAN-10-0749. Epub 2010 Oct 19.
  PUBMED    20959491
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-10-0749"; journalName: "Cancer Res"; date: 
            "2010-11-1- 1"; volume: "70"; issue: "21"; pages: "8264-9"
COMMENT     Sequence Label: pCAG-luciferase
FEATURES             Location/Qualifiers
     source          1..7330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        36..415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          693..1709
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1633..1655
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1717..1736
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1825..1834
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1831..3483
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3527..3648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3538..3557)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3592..3611
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3842..4199
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3842..4033
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3865..3885)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4050..4185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4112..4131
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4230..5024
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5049..5097
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5253..5270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5424..6012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5504..5523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6212..7003,7004..7072))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    7177..7225
                     /note="synthetic polyadenylation signal"
     misc_feature    7239..7330
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     7279..7298
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgacat tgattattga ctagttatta
       61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      241 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga
      421 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt
      481 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcg
      541 cgccrggggg gggggggsgg ggsgrggggs ggggsggggs grggsggrgr ggkgcggcgg
      601 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
      661 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg
      721 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
      781 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
      841 gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt
      901 tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
      961 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     1021 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
     1081 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
     1141 gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc
     1201 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
     1261 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg grgggctcgg
     1321 gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
     1381 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
     1441 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
     1501 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     1561 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
     1621 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     1681 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     1741 ctcatcattt tggcaaagaa ttcctcgagg atatcaagat ctggcctcgg cggccaagct
     1801 tggcaatccg gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc
     1861 ccagcgccat tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg
     1921 aagcgctacg ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac
     1981 attacctacg ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat
     2041 gggctgaata caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg
     2101 cccgtgttgg gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac
     2161 gagcgcgagc tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag
     2221 aaagggctgc aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc
     2281 atcatggata gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc
     2341 catttgccac ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa
     2401 accatcgccc tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta
     2461 ccgcaccgca ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag
     2521 atcatccccg acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc
     2581 accacgctgg gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag
     2641 gagctattct tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca
     2701 ctatttagct tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac
     2761 gagatcgcca gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc
     2821 ttccacctac caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg
     2881 atcacccccg aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag
     2941 gctaaggtgg tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg
     3001 tgcgtccgtg gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct
     3061 ctcatcgaca aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag
     3121 cacttcttca tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc
     3181 ccagccgaac tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc
     3241 ggcctgcccg acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt
     3301 aaaaccatga ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag
     3361 aagctgcgcg gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg
     3421 gacgcccgca agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg
     3481 taataattct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat
     3541 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     3601 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     3661 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     3721 aacctctaca aatgtggtaa aatcgataag gatccgtttg cgtattgggc gctcttccgc
     3781 tgatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttagctacct
     3841 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3901 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3961 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4021 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4081 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     4141 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4201 agctcgattc ttctgacact agcgccacca tgatcgaaca agacggcctc catgctggca
     4261 gtcccgcagc ttgggtcgaa cgcttgttcg ggtacgactg ggcccagcag accatcggat
     4321 gtagcgatgc ggccgtgttc cgtctaagcg ctcaaggccg gcccgtgctg ttcgtgaaga
     4381 ccgacctgag cggcgccctg aacgagcttc aagacgaggc tgcccgcctg agctggctgg
     4441 ccaccaccgg cgtaccctgc gccgctgtgt tggatgttgt gaccgaagcc ggccgggact
     4501 ggctgctgct gggcgaggtc cctggccagg atctgctgag cagccacctt gcccccgctg
     4561 agaaggtttc tatcatggcc gatgcaatgc ggcgcctgca caccctggac cccgctacct
     4621 gccccttcga ccaccaggct aagcatcgga tcgagcgtgc tcggacccgc atggaggccg
     4681 gcctggtgga ccaggacgac ctggacgagg agcatcaggg cctggccccc gctgaactgt
     4741 tcgcccgact gaaagcccgc atgccggacg gtgaggacct ggttgtcaca cacggagatg
     4801 cctgcctccc taacatcatg gtcgagaatg gccgcttctc cggcttcatc gactgcggtc
     4861 gcctaggagt tgccgaccgc taccaggaca tcgccctggc cacccgcgac atcgctgagg
     4921 agcttggcgg cgagtgggcc gaccgcttct tagtcttgta cggcatcgca gctcccgaca
     4981 gccagcgcat cgccttctac cgcttgctcg acgagttctt ttaatgatct agaaccggtc
     5041 atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgttc
     5101 gaactagatg ctgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     5161 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     5221 taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5281 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5341 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5401 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5461 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5521 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5581 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5641 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5701 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5761 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5821 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5881 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5941 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6001 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6061 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6121 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6181 agcggccgca aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga
     6241 tctcagcgat ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca
     6301 ctacgattcg tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc
     6361 gttcaccggc ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa
     6421 gtggtcctgc tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag
     6481 taagaagttc gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg
     6541 tatcacgctc gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg
     6601 tcacatgatc acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg
     6661 tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc
     6721 ttaccgtcat gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt
     6781 tttgtgagta gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca
     6841 ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga
     6901 aagactcaag gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca
     6961 gttgatcttc agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc
     7021 aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg ttggatgctc atactcgtcc
     7081 tttttcaata ttattgaagc atttatcagg gttactagta cgtctctcaa ggataagtaa
     7141 gtaatattaa ggtacgggag gtattggaca ggccgcaata aaatatcttt attttcatta
     7201 catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac
     7261 aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga
     7321 acatttctct
//
