LOCUS       sequence-45158-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Sims JJ, Scavone F, Cooper EM, Kane LA, Youle RJ, Boeke JD, Cohen RE
  TITLE     Polyubiquitin-sensor proteins reveal localization and linkage-type 
            dependence of cellular ubiquitin signaling.
  JOURNAL   Nat Methods. 2012 Feb 5. doi: 10.1038/nmeth.1888.
  PUBMED    22306808
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Feb 5. doi: 10.1038/nmeth.1888."
COMMENT     Sequence Label: pQCXIP-Vx3NB-mEGFP
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(262..288)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(262..288)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      297..306
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(427..451)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(448..651)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(477..497)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggtggcgacc ggtggatccc cagcagagtt accgccctcc ttcagctcca gctcgatctc
       61 cttgcgctcc agctcctcct cgtcctcttc ctgagcgtta acttcacgag attctttcag
      121 ctccagttcg atctctttac gctccagttc ttcttcgtct tctttaactt tttcttcagc
      181 acgagcttca cgcagctctt cctggatctc agctttctca tcaggatctt cttcttcttc
      241 catctcgagg ccgctgccgc cggcgtagtc gggcacgtcg taggggtagc tgccctccat
      301 ggtggcggcg cggccgcgga tcaattcctg caggtccgtt ccaatgcacc gttcccggcc
      361 gcggaggctg gatcggtccc ggtgtcttct atggaggtca aaacagcgtg gatggcgtct
      421 ccaggcgatc tgacggttca ctaaacgagc tctgcttata tagacctccc accgtacacg
      481 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
      541 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
      601 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat
//
