LOCUS       sequence-451887-        9632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to transiently express Myc-tagged CRaf with the pErk reporter. 
            Used in LABEL-seq MAPK activity assays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Simon JJ, Fowler DM, Maly DJ
  TITLE     Multiplexed profiling of intracellular protein abundance, activity, 
            interactions and druggability with LABEL-seq.
  JOURNAL   Nat Methods. 2024 Nov;21(11):2094-2106. doi: 
            10.1038/s41592-024-02456-7. Epub 2024 Oct 21.
  PUBMED    39433876
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-024-02456-7"; journalName: "Nat Methods"; date: 
            "2024-11"; volume: "21"; issue: "11"; pages: "2094-2106"
COMMENT     Sequence Label: CRaf-IRES-pErk reporter
FEATURES             Location/Qualifiers
     source          1..9632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(404..1195,1196..1264))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1027..1046
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1265..1369)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1444..1463)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1635..2014
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2015..2218
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2169..2189
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2220..2238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2241..2259
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2269..2293
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2387..2396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2396..2425
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    join(4458..5032,5033..5035,5036..5044)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4625..4642)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4852..4871
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5048..5113
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6914..6949
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
     primer_bind     complement(6984..7001)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6990..7214
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7347..7366)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7498..7545
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7553..8575
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7553..8575
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8705..8826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8742..8761)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8796..8815
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8875..8891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8875..8891)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8888..8910)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8899..8915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8923..8929,8930..8947,8948..8953))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8968..8989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9106..9123)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9277..9632,1..233))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9357..9376)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
       61 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      121 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      181 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      241 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
      301 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      361 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      421 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
      481 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
      541 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
      601 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
      661 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
      721 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
      781 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
      841 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
      901 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
      961 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1021 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1081 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1141 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1201 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1261 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1321 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1381 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     1441 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     1501 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     1561 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     1621 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     1681 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1741 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1801 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     1861 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     1921 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     1981 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     2041 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2101 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2161 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
     2221 ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt
     2281 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
     2341 ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttgccgcca ccatggaaca
     2401 aaaactcatc tcagaagagg atctcggtgg cagcggtagt ggatctggct caggaatgga
     2461 gcacatacag ggagcttgga agacgatcag caatggtttt ggattcaaag atgccgtgtt
     2521 tgatggctcc agctgcatct ctcctacaat agttcagcag tttggctatc agcgccgggc
     2581 atcagatgat ggcaaactca cagatccttc taagacaagc aacactatcc gtgttttctt
     2641 gccgaacaag caaagaacag tggtcaatgt gcgaaatgga atgagcttgc atgactgcct
     2701 tatgaaagca ctcaaggtga ggggcctgca accagagtgc tgtgcagtgt tcagacttct
     2761 ccacgaacac aaaggtaaaa aagcacgctt agattggaat actgatgctg cgtctttgat
     2821 tggagaagaa cttcaagtag atttcctgga tcatgttccc ctcacaacac acaactttgc
     2881 tcggaagacg ttcctgaagc ttgccttctg tgacatctgt cagaaattcc tgctcaatgg
     2941 atttcgatgt cagacttgtg gctacaaatt tcatgagcac tgtagcacca aagtacctac
     3001 tatgtgtgtg gactggagta acatcagaca actcttattg tttccaaatt ccactattgg
     3061 tgatagtgga gtcccagcac taccttcttt gactatgcgt cgtatgcgag agtctgtttc
     3121 caggatgcct gttagttctc agcacagata ttctacacct cacgccttca cctttaacac
     3181 ctccagtccc tcatctgaag gttccctctc ccagaggcag aggtcgacat ccacacctaa
     3241 tgtccacatg gtcagcacca ccctgcctgt ggacagcagg atgattgagg atgcaattcg
     3301 aagtcacagc gaatcagcct caccttcagc cctgtccagt agccccaaca atctgagccc
     3361 aacaggctgg tcacagccga aaacccccgt gccagcacaa agagagcggg caccagtatc
     3421 tgggacccag gagaaaaaca aaattaggcc tcgtggacag agagattcaa gctattattg
     3481 ggaaatagaa gccagtgaag tgatgctgtc cactcggatt gggtcaggct cttttggaac
     3541 tgtttataag ggtaaatggc acggagatgt tgcagtaaag atcctaaagg ttgtcgaccc
     3601 aaccccagag caattccagg ccttcaggaa tgaggtggct gttctgcgca aaacacggca
     3661 tgtgaacatt ctgcttttca tggggtacat gacaaaggac aacctggcaa ttgtgaccca
     3721 gtggtgcgag ggcagcagcc tctacaaaca cctgcatgtc caggagacca agtttcagat
     3781 gttccagcta attgacattg cccggcagac ggctcaggga atggactatt tgcatgcaaa
     3841 gaacatcatc catagagaca tgaaatccaa caatatattt ctccatgaag gcttaacagt
     3901 gaaaattgga gattttggtt tggcaacagt aaagtcacgc tggagtggtt ctcagcaggt
     3961 tgaacaacct actggctctg tcctctggat ggccccagag gtgatccgaa tgcaggataa
     4021 caacccattc agtttccagt cggatgtcta ctcctatggc atcgtattgt atgaactgat
     4081 gacgggggag cttccttatt ctcacatcaa caaccgagat cagatcatct tcatggtggg
     4141 ccgaggatat gcctccccag atcttagtaa gctatataag aactgcccca aagcaatgaa
     4201 gaggctggta gctgactgtg tgaagaaagt aaaggaagag aggcctcttt ttccccagat
     4261 cctgtcttcc attgagctgc tccaacactc tctaccgaag atcaaccgga gcgcttccga
     4321 gccatccttg catcgggcag cccacactga ggatatcaat gcttgcacgc tgaccacgtc
     4381 cccgaggctg cctgtcttct gactcgaggg gatccaccgg atctagataa ctgatcactg
     4441 tggaatcaga gagactgccc ctctccctct ccccccccta acgttactgg ccgaagccgc
     4501 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     4561 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     4621 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     4681 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     4741 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     4801 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     4861 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     4921 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     4981 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
     5041 aaccatggat tataaagatc atgacgggga ctataaggat cacgatattg actacaagga
     5101 tgacgacgac aaaggttctg gcagtggcag cggagcttct aactttactc agttcgttct
     5161 cgtcgacaat ggcggaactg gcgacgtgac tgtcgcccca agcaacttcg ctaacgggat
     5221 cgctgaatgg atcagctcta actcgcgttc acaggcttac aaagtaacct gtagcgttcg
     5281 tcagagctct gcgcagaatc gcaaatacac catcaaagtc gaggtgccta aaggcgcctg
     5341 gcgttcgtac ttaaatatgg aactaaccat tccaattttc gccacgaatt ccgactgcga
     5401 gcttattgtt aaggcaatgc aaggtctcct aaaagatgga aacccgattc cctcagcaat
     5461 cgcagcaaac tccggcatct acgcaatggc ttctaacttt actcagttcg ttctcgtcga
     5521 caatggcgga actggcgacg tgactgtcgc cccaagcaac ttcgctaacg ggatcgctga
     5581 atggatcagc tctaactcgc gttcacaggc ttacaaagta acctgtagcg ttcgtcagag
     5641 ctctgcgcag aatcgcaaat acaccatcaa agtcgaggtg cctaaaggcg cctggcgttc
     5701 gtacttaaat atggaactaa ccattccaat tttcgccacg aattccgact gcgagcttat
     5761 tgttaaggca atgcaaggtc tcctaaaaga tggaaacccg attccctcag caatcgcagc
     5821 aaactccggc atctacggct ccggaggctc tggtagcgga tccggtggca tggcggcagc
     5881 cgcagcggca ggggctgggc cagagatggt ccgcgggcag gtgttcgacg tggggccgcg
     5941 ctacaccaac ctctcgtaca tcggcgaggg cgcctacggc atggtgtgct ctgcttatga
     6001 taatgtcaac aaagttcgag tagctatcaa gaaaatcagc ccctttgagc accagaccta
     6061 ctgccagaga accctgaggg agataaaaat cttactgcgc ttcagacatg agaacatcat
     6121 tggaatcaat gacattattc gagcaccaac catcgagcaa atgaaagatg tatatatagt
     6181 acaggacctc atggaaacag atctttacaa gctcttgaag acacaacacc tcagcaatga
     6241 ccatatctgc tattttctct accagatcct cagagggtta aaatatatcc attcagctaa
     6301 cgttctgcac cgtgacctca agccttccaa cctgctgctc aacaccacct gtgatctcaa
     6361 gatctgtgac tttggcctgg cccgtgttgc agatccagac catgatcaca cagggttcct
     6421 gacagaatat gtggccacac gttggtacag ggctccagaa attatgttga attccaaggg
     6481 ctacaccaag tccattgata tttggtctgt aggctgcatt ctggcagaaa tgctttctaa
     6541 caggcccatc tttccaggga agcattatct tgaccagctg aaccacattt tgggtattct
     6601 tggatcccca tcacaagaag acctgaattg tataataaat ttaaaagcta ggaactattt
     6661 gctttctctt ccacacaaaa ataaggtgcc atggaacagg ctgttcccaa atgctgactc
     6721 caaagctctg gacttattgg acaaaatgtt gacattcaac ccacacaaga ggattgaagt
     6781 agaacaggct ctggcccacc catatctgga gcagtattac gacccgagtg acgagcccat
     6841 cgccgaagca ccattcaagt tcgacatgga attggatgac ttgcctaagg aaaagctcaa
     6901 agaactaatt tttgaagaga ctgctagatt ccagccagga tacagatctt aatctagagg
     6961 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     7021 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     7081 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     7141 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     7201 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     7261 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     7321 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     7381 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     7441 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtacctagaa
     7501 gttcctattc cgaagttcct attctctaga aagtatagga acttccttgg ccaaaaagcc
     7561 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     7621 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     7681 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     7741 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     7801 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     7861 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     7921 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     7981 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     8041 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     8101 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     8161 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     8221 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     8281 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     8341 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     8401 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     8461 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     8521 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt
     8581 actacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8641 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8701 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8761 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8821 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8881 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8941 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9001 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9061 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9121 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9181 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9241 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9301 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9361 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9421 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9481 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9541 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9601 aagacacgac ttatcgccac tggcagcagc ca
//
