LOCUS       sequence-451930-        9205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mU6-driven expression of RfxCas13d gRNAs and arrays. Contains AarI 
            sites for guide cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   El Kassem G, Hillmer J, Boettcher M
  TITLE     Evaluation of Cas13d as a tool for genetic interaction mapping.
  JOURNAL   Nat Commun. 2025 Feb 14;16(1):1631. doi: 10.1038/s41467-025-56747-4.
  PUBMED    39952934
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-02-14"; volume: "16(1):1631. doi"; pages: " 
            10.1038/s41467-025-56747-4"
COMMENT     Sequence Label: 783-Rx-mU6: RfxCas13d gRNA and array cloning
            b...
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        376..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     529..549
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             592..772
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    819..944
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1441..1674
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1859..1903
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2201..2318
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2571..2604)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2608..2628
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        4126..4337
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4362..4958
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4362..4381)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4858..4878
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4968..5021
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      5028..5037
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5034..5741
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5179..5197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5447..5466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5751..5771
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5851..6439
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5904..5924)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6322..6333)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6442..6458)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6443..6459)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6968..7148
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7210..7798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7290..7309)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7969..8760,8761..8829))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8592..8611
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8830..8934)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9009..9028)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9200..9205,1..374)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
       61 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      121 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      181 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      241 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      301 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      361 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      421 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      481 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      541 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      601 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      661 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      721 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      781 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      841 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      901 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      961 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1021 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1081 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1141 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1201 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1261 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     1321 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1381 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1441 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1501 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1561 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1621 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1681 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1741 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1801 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1861 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1921 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1981 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2041 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2101 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     2161 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     2221 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     2281 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     2341 gagatccagt ttggttagta ccgggcccgc tctagagatc cgacgcgcca tctctaggcc
     2401 cgcgccggcc ccctcgcacg gacttgtggg agaagctcgg ctactcccct gccccggtta
     2461 atttgcatat aatatttcct agtaactata gaggcttaat gtgcgataaa agacagataa
     2521 tctgttcttt ttaatactag ctacatttta catgataggc ttggatttct ataacttcgt
     2581 atagcataca ttatacgaag ttataaacag cacaaaagga aactcaccct aactgtaaag
     2641 taattgtgtg ttttgagact ataagtatcc cttggagaac caccttgttg gagacgcagg
     2701 tgtcactggc gctgttggtg ccggcacctg acacagcaga ggatgcagag cccaaggaaa
     2761 cccagaaagc cgaaggagcc acccagcacg caagccagga acactgtctg gttcacccct
     2821 ctggataaaa cctggatcct ggagtccatc ttgaccttat caccttcact cagtagacac
     2881 tgccacagcc ctgtctcagg ggccaccact tgaactactt tctgctcctc agagaccctg
     2941 gcctcctggt tctcctgctt cagggtcagt ctcatcttgg gagaggtagg tcccatcacc
     3001 tcacaggtca aagtattgtt gagctgagcc actttcatca ccaccaggtt cacttcctga
     3061 tgcagtgtcc ctttgtccag agtcagagtc aggttgccag aaccagcaaa ctgaagcgag
     3121 acctggggta tcttgagggt gagtgggagc gtttccttca gctggagctt gaggtctttg
     3181 gtggactttt gtacggacac ctctttgttc tttatggaga aggagatcca gggctggaag
     3241 aaagaatcct tctctgcctt ccacatcagc tctccccacc cgttttcctc tgcaaagttg
     3301 agtgggaagg agaactccgc tgactctccc tcactcttat aggccgtgat agctgtgctc
     3361 tgaaaaccca gcactgagag tgtcatgccg aaccagttct ttttctggtc cagggtcacg
     3421 gtgcagttcc agaagtcgct gtcctgaacc cttaggttgg acatggagag aactttggaa
     3481 ccactgacaa ctttaccctt tttgtgtttg cactctgtca aggggttaga gaccttagag
     3541 ttgctatcca aggtcagggt caggctctgc ccttgcaaca ggctggtacc cggactgaag
     3601 gtcactttga acacccacaa ctccacctcc tctttcctgt tctccagctc acagatataa
     3661 gtctgagagt cttccatctt aagtttattg atgatgagag gaaacgatcc tttctcccat
     3721 gccccttttt tggaatcaaa acgatcaaac tgcgaaggcg aacctcctct aattaataca
     3781 cctttgccat gctgccccag aatcttcctc tggtcagaga acttccaggt gaagactgtg
     3841 atcttcttct gggaactctc gcagggcagt tctgctgatt ccccttcctt ccccagcacc
     3901 agcgtcttcc cttgagtgac agctaggagt tgtgacagct gcagcagcag cagcagcaag
     3961 cgcctaagag agatggctcg gcacatgtca cctgcggctt taaggccggt cctagcaatt
     4021 tttttgcggc cgcgtggata accgtattac cgccatgcag cacacgtctg aactccagtc
     4081 actaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
     4141 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa
     4201 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     4261 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     4321 ttgccgccag aacacaggac cggttctaga gcgctgccac catgaccgag tacaagccca
     4381 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     4441 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     4501 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     4561 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     4621 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     4681 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     4741 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     4801 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     4861 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     4921 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg atcgggagag ggcagaggaa
     4981 gtctgctaac atgcggtgac gtcgaggaga atcctggccc accggtcgcc accatggtga
     5041 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     5101 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     5161 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     5221 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     5281 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     5341 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     5401 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     5461 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     5521 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     5581 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     5641 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     5701 agggccgcca ctccaccggc ggcatggacg agctgtacaa gggagctgat ccaaaaaaga
     5761 agagaaaggt atagttaatt aagtcgaggg acctaacatg tttaagggtt ccggttccac
     5821 taggtacaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     5881 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5941 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6001 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6061 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6121 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6181 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6241 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6301 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6361 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6421 ctttgggccg cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg
     6481 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     6541 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     6601 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6661 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     6721 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     6781 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     6841 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     6901 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     6961 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7021 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7081 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7141 ctctagcagc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7201 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7261 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7321 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7381 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7441 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7501 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7561 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7621 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7681 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7741 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7801 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7861 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7921 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7981 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8041 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8101 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8161 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8221 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8281 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8341 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8401 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8461 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8521 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8581 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8641 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8701 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8761 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8821 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8881 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8941 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     9001 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     9061 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     9121 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     9181 cgggccagat atacgcgttg acatt
//
