LOCUS       sequence-45224-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8NmCherry-ABCB4a
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             102..809
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(247..265)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     515..534
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatccacta gtcatcgatt gatggtgagc aagggcgagg
      121 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
      181 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
      241 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
      301 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
      361 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
      421 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
      481 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
      541 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
      601 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
      661 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
      721 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
      781 ccaccggcgg catggacgag ctgtacaagg aattcggagc cggcgc
//
