LOCUS       sequence-452347-        8700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FLAG-tagged fluorescent proteins for 
            immunohistochemical detection in FFPE tissue.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Sharma VP, Tang B, Wang Y, Duran CL, Karagiannis GS, Xue EA, 
            Entenberg D, Borriello L, Coste A, Eddy RJ, Kim G, Ye X, Jones JG, 
            Grunblatt E, Agi N, Roy S, Bandyopadhyaya G, Adler E, Surve CR, 
            Esposito D, Goswami S, Segall JE, Guo W, Condeelis JS, Wakefield LM,
            Oktay MH
  TITLE     Live tumor imaging shows macrophage induction and TMEM-mediated 
            enrichment of cancer stem cells during metastatic dissemination.
  JOURNAL   Nat Commun. 2021 Dec 15;12(1):7300. doi: 10.1038/s41467-021-27308-2.
  PUBMED    34911937
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-12-15"; volume: "12(1):7300. doi"; pages: " 
            10.1038/s41467-021-27308-2"
COMMENT     Sequence Label: 18065-M02-412
FEATURES             Location/Qualifiers
     source          1..8700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1941..1961
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(2206..2226)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2225..2263
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2260..2284
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2345..2369
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2375..2440
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2441..3145
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2583..2601)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2851..2870
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3270..3294)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3308..3896
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3361..3381)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3779..3790)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3947..3967)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3991..4201
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4013..4209
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4060..4195
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4257..4304
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    complement(5347..5367)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     LTR             5436..5669
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5741..5862
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5778..5797)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5832..5851
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5902..6037
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6070..6086)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6070..6086)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6083..6105)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6094..6110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6118..6124,6125..6142,6143..6148))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6163..6184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6301..6318)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6472..7060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6552..6571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7231..8022,8023..8091))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7854..7873
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8092..8196)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8264..8282
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8320..8342)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8442..8461
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8655..8677
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8669..8686
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8670..8686
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgatgaattc ccgctcgaat caactttgta tagaaaagtt ggccaaatta caaaattcaa
     1981 aattttatcg atcagctact tttgcattac aatggccttg gtgcagctac ttttgcatta
     2041 caatggcctt ggtgcagcta cttttgcatt acaatggcct tggtggaatt ccagctactt
     2101 ttgcattaca atggccttgg tgcagctact tttgcattac aatggccttg gtgcagctac
     2161 ttttgcatta caatggcctt ggtgactagt taggcgtgta cgggccaact tttgtataca
     2221 aagtggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     2281 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     2341 cagcacaact ttgtacaaaa aagttggcac catggactac aaagaccatg acggtgatta
     2401 taaggatcac gacatcgatt acaaggatga cgatgacaag gtgagcaagg gcgaggagga
     2461 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     2521 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     2581 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     2641 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     2701 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     2761 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     2821 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     2881 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     2941 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     3001 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     3061 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     3121 cggcggcatg gacgagctgt acaagcttag ccatggcttc ccgccggcgg tggcggcgca
     3181 ggatgatggc acgctgccca tgtcttgtgc ccaggagagc gggatggacc gtcaccctgc
     3241 agcctgtgct tctgctagga tcaatgtgta atcaactttc ttgtacaaag ttgtgattcg
     3301 actcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3361 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3421 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3481 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3541 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3601 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3661 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     3721 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3781 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3841 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     3901 aattctgcag atatccggtt agtaatgagt ttggaattaa ttctgtggaa tgtgtgtcag
     3961 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4021 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     4081 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4141 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     4201 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     4261 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     4321 ccatgccacc tcctcgcctc ctcttcttcc tcctcttcct cacccccatg gaagtcaggc
     4381 ccgaggaacc tctagtggtg aaggtggaag agggagataa cgctgtgctg cagtgcctca
     4441 aggggacctc agatggcccc actcagcagc tgacctggtc tcgggagtcc ccgcttaaac
     4501 ccttcttaaa actcagcctg gggctgccag gcctgggaat ccacatgagg cccctggcca
     4561 tctggctttt catcttcaac gtctctcaac agatgggggg cttctacctg tgccagccgg
     4621 ggcccccctc tgagaaggcc tggcagcctg gctggacagt caatgtggag ggcagcgggg
     4681 agctgttccg gtggaatgtt tcggacctag gtggcctggg ctgtggcctg aagaacaggt
     4741 cctcagaggg ccccagctcc ccttccggga agctcatgag ccccaagctg tatgtgtggg
     4801 ccaaagaccg ccctgagatc tgggagggag agcctccgtg tctcccaccg agggacagcc
     4861 tgaaccagag cctcagccag gacctcacca tggcccctgg ctccacactc tggctgtcct
     4921 gtggggtacc ccctgactct gtgtccaggg gccccctctc ctggacccat gtgcacccca
     4981 aggggcctaa gtcattgctg agcctagagc tgaaggacga tcgcccggcc agagatatgt
     5041 gggtaatgga gacgggtctg ttgttgcccc gggccacagc tcaagacgct ggaaagtatt
     5101 attgtcaccg tggcaacctg accatgtcat tccacctgga gatcactgct cggccagtac
     5161 tatggcactg gctgctgagg actggtggct ggaaggtctc agctgtgact ttggcttatc
     5221 tgatcttctg cctgtgttcc cttgtgggca ttcttcatct tcaaagagcc ctggtcctga
     5281 ggaggaaaag aaagcgaatg actgacccca ccaggagatt ctaagcacaa ttcgagctcg
     5341 gtacctcaac tttattatac atagttgatc tttaagacca atgacttaca aggcagctgt
     5401 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     5461 aagataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5521 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5581 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5641 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     5701 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     5761 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5821 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     5881 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5941 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6001 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt
     6061 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6121 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6181 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6241 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6901 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7201 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7261 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7321 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7381 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7441 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7501 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7561 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7621 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7681 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7741 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7801 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7861 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7921 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7981 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8041 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8101 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8161 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8221 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     8281 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     8341 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     8401 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     8461 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     8521 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     8581 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     8641 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctgacgcg
//
