LOCUS       sequence-45284-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt6
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..54)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    80..104
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctaaccggta cgcgtagaat cgagaccgag gagagggtta gggataggct taccttcgaa
       61 ccgcgggccc tctagatcaa ccactttgta caagaaagct gggtcggcgc gcccaccctt
      121 cagaattcac aggcagaggc tgagctcctt gcgcacacgg cagcggtggc actgtactac
      181 gcagcaccag tggaagcggc acaggcagtt ctcttcgagc tgcacgctct cctggcggtg
      241 cccgcggccg cagcacagca ggtcgcagcc gctgaggtcc ggggcgctgc tattgcaggc
      301 gcgaccgcgc gtgccggggg agccggtgcg tcggttgggg gcgcagaagt cgggcgaatc
      361 ggcggcgtag aggaggtccg ctcggcccgg cggcttgagc gtgcggacgg cgggcagcag
      421 ggccttgccg tcgttggtgc ccatgacgcg tgaggcgccg tggaagcgct ccagcagccg
      481 cgcgcccacc tcgcgaaatg gaggcagctt ctgccagcag gtgcgcagcg cgcatgatcc
      541 cgacagcccg tggcatttgc actcggtgcg cgtgtggctc cgcacggcca gcctgcccgc
      601 ctcgttgttg tgcagttgca ccaacgcgcg gatgtctccg cgtccccgct tgtgccgtgc
      661 gtccataaag agcctcgact tctcgtcccc gaagtccacg tcgtcgccgc agcctcccca
      721 ctcccaggcg gcgctgcctt ccggggagcc cgcggggcca gggggtccgg gggtgccggg
      781 caggccggag ggccggggag gggcccgccc gcggggcgcc tggcagccgc ac
//
