LOCUS       sequence-453528-       23148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for dSpCas9 mediated gene activation for dicot plants; 
            NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was 
            driven by AtU3 promoter; BASTA for plants selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23148)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 23148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT56
FEATURES             Location/Qualifiers
     source          1..23148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    283..307
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     510..526
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        542..2539
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    2582..2606
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2628..2693
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2700..2720
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2745..2768,2769..2771,2772..5258,5259..5261,
                     5262..6845)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6758..6766
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6846..6893
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7071..7124
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7128..7514
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             7515..7535
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7578..7604
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7584..7610
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             7623..7643
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7650..7670
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7734..7790
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7806..7862
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7878..7934
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7950..8006
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8022..8078
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8094..8150
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8166..8222
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8238..8294
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8310..8366
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8382..8438
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             8520..8540
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        9180..11177
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             11925..11951
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             12042..12752
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             13137..13298
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             13326..13376
                     /codon_start=1
                     /product="nuclear localization signal from the human T-cell
                     leukemia virus type 1 (HTLV-1) Rex protein"
                     /label=Rex NLS
                     /translation="PKTRRRPRRSQRKRPPT"
     protein_bind    14047..14067
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(14428..14733)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGL"
     primer_bind     14598..14614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14624..14643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14624..14642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14690..14710)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(14690..14708)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14729..14745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    14753..14769
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14777..14783,14784..14801,14802..14807))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14822..14843
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        14902..14920
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        14972..14990
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     protein_bind    complement(15223..15247)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(15315..15331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    15339..15355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15363..15369,15370..15387,15388..15393))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15408..15429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        15620..16296
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     16233..16253
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             16341..16892
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    16899..17073
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    17151..17175
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             17600..18394
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      18481..19069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     18970..18989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     19223..19240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    19255..19395
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(19321..19340)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     19481..19503
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      19739..19933
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(19999..21072)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(21501..22130)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
       61 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
      121 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
      181 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
      241 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
      301 cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa
      361 gctgctctag cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
      421 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      481 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgcatg
      541 cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct
      601 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
      661 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
      721 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
      781 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
      841 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
      901 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
      961 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
     1021 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
     1081 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
     1141 acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag
     1201 acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg
     1261 gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca
     1321 cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac
     1381 cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc
     1441 tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc
     1501 acccgtcggc acctccgctt caaggtacgc cgctcgtccc cccccccccc cccctctcta
     1561 ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat
     1621 gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg
     1681 acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct
     1741 gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc
     1801 atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg
     1861 tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt
     1921 tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat taattttgga
     1981 tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg atggaaatat
     2041 cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac agagatgctt
     2101 tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc gttctagatc
     2161 ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac tgtatgtgtg
     2221 tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc taggataggt
     2281 atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca gcatctattc
     2341 atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt ataattattt
     2401 tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt tttttagccc
     2461 tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct caccctgttg
     2521 tttggtgtta cttctgcagg tcgactctag aggatcccct cgaggcgcgc caagctatca
     2581 aacaagtttg tacaaaaaag caggctccga attcgccctt caccatggat tacaaggacc
     2641 acgacgggga ttacaaggac cacgacattg attacaagga tgatgatgac aagatggctc
     2701 cgaagaagaa gaggaaggtt ggcatccacg gggtgccagc tgctgacaag aagtactcga
     2761 tcggcctcgc tattgggact aactctgttg gctgggccgt gatcaccgac gagtacaagg
     2821 tgccctcaaa gaagttcaag gtcctgggca acaccgatcg gcattccatc aagaagaatc
     2881 tcattggcgc tctcctgttc gacagcggcg agacggctga ggctacgcgg ctcaagcgca
     2941 ccgcccgcag gcggtacacg cgcaggaaga atcgcatctg ctacctgcag gagattttct
     3001 ccaacgagat ggcgaaggtt gacgattctt tcttccacag gctggaggag tcattcctcg
     3061 tggaggagga taagaagcac gagcggcatc caatcttcgg caacattgtc gacgaggttg
     3121 cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg gactccacag
     3181 ataaggcgga cctccgcctg atctacctcg ctctggccca catgattaag ttcaggggcc
     3241 atttcctgat cgagggggat ctcaacccgg acaatagcga tgttgacaag ctgttcatcc
     3301 agctcgtgca gacgtacaac cagctcttcg aggagaaccc cattaatgcg tcaggcgtcg
     3361 acgcgaaggc tatcctgtcc gctaggctct cgaagtctcg gcgcctcgag aacctgatcg
     3421 cccagctgcc gggcgagaag aagaacggcc tgttcgggaa tctcattgcg ctcagcctgg
     3481 ggctcacgcc caacttcaag tcgaatttcg atctcgctga ggacgccaag ctgcagctct
     3541 ccaaggacac atacgacgat gacctggata acctcctggc ccagatcggc gatcagtacg
     3601 cggacctgtt cctcgctgcc aagaatctgt cggacgccat cctcctgtct gatattctca
     3661 gggtgaacac cgagattacg aaggctccgc tctcagcctc catgatcaag cgctacgacg
     3721 agcaccatca ggatctgacc ctcctgaagg cgctggtcag gcagcagctc cccgagaagt
     3781 acaaggagat cttcttcgat cagtcgaaga acggctacgc tgggtacatt gacggcgggg
     3841 cctctcagga ggagttctac aagttcatca agccgattct ggagaagatg gacggcacgg
     3901 aggagctgct ggtgaagctc aatcgcgagg acctcctgag gaagcagcgg acattcgata
     3961 acggcagcat cccacaccag attcatctcg gggagctgca cgctatcctg aggaggcagg
     4021 aggacttcta ccctttcctc aaggataacc gcgagaagat cgagaagatt ctgactttca
     4081 ggatcccgta ctacgtcggc ccactcgcta ggggcaactc ccgcttcgct tggatgaccc
     4141 gcaagtcaga ggagacgatc acgccgtgga acttcgagga ggtggtcgac aagggcgcta
     4201 gcgctcagtc gttcatcgag aggatgacga atttcgacaa gaacctgcca aatgagaagg
     4261 tgctccctaa gcactcgctc ctgtacgagt acttcacagt ctacaacgag ctgactaagg
     4321 tgaagtatgt gaccgagggc atgaggaagc cggctttcct gtctggggag cagaagaagg
     4381 ccatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcag ctcaaggagg
     4441 actacttcaa gaagattgag tgcttcgatt cggtcgagat ctctggcgtt gaggaccgct
     4501 tcaacgcctc cctggggacc taccacgatc tcctgaagat cattaaggat aaggacttcc
     4561 tggacaacga ggagaatgag gatatcctcg aggacattgt gctgacactc actctgttcg
     4621 aggaccggga gatgatcgag gagcgcctga agacttacgc ccatctcttc gatgacaagg
     4681 tcatgaagca gctcaagagg aggaggtaca ccggctgggg gaggctgagc aggaagctca
     4741 tcaacggcat tcgggacaag cagtccggga agacgatcct cgacttcctg aagagcgatg
     4801 gcttcgcgaa ccgcaatttc atgcagctga ttcacgatga cagcctcaca ttcaaggagg
     4861 atatccagaa ggctcaggtg agcggccagg gggactcgct gcacgagcat atcgcgaacc
     4921 tcgctggctc gccagctatc aagaagggga ttctgcagac cgtgaaggtt gtggacgagc
     4981 tggtgaaggt catgggcagg cacaagcctg agaacatcgt cattgagatg gcccgggaga
     5041 atcagaccac gcagaagggc cagaagaact cacgcgagag gatgaagagg atcgaggagg
     5101 gcattaagga gctggggtcc cagatcctca aggagcaccc ggtggagaac acgcagctgc
     5161 agaatgagaa gctctacctg tactacctcc agaatggccg cgatatgtat gtggaccagg
     5221 agctggatat taacaggctc agcgattacg acgtcgatgc tatcgttcca cagtcattcc
     5281 tgaaggatga ctccattgac aacaaggtcc tcaccaggtc ggacaagaac cggggcaagt
     5341 ctgataatgt tccttcagag gaggtcgtta agaagatgaa gaactactgg cgccagctcc
     5401 tgaatgccaa gctgatcacg cagcggaagt tcgataacct cacaaaggct gagaggggcg
     5461 ggctctctga gctggacaag gcgggcttca tcaagaggca gctggtcgag acacggcaga
     5521 tcactaagca cgttgcgcag attctcgact cacggatgaa cactaagtac gatgagaatg
     5581 acaagctgat ccgcgaggtg aaggtcatca ccctgaagtc aaagctcgtc tccgacttca
     5641 ggaaggattt ccagttctac aaggttcggg agatcaacaa ttaccaccat gcccatgacg
     5701 cgtacctgaa cgcggtggtc ggcacagctc tgatcaagaa gtacccaaag ctcgagagcg
     5761 agttcgtgta cggggactac aaggtttacg atgtgaggaa gatgatcgcc aagtcggagc
     5821 aggagattgg caaggctacc gccaagtact tcttctactc taacattatg aatttcttca
     5881 agacagagat cactctggcc aatggcgaga tccggaagcg ccccctcatc gagacgaacg
     5941 gcgagacggg ggagatcgtg tgggacaagg gcagggattt cgcgaccgtc aggaaggttc
     6001 tctccatgcc acaagtgaat atcgtcaaga agacagaggt ccagactggc gggttctcta
     6061 aggagtcaat tctgcctaag cggaacagcg acaagctcat cgcccgcaag aaggactggg
     6121 atccgaagaa gtacggcggg ttcgacagcc ccactgtggc ctactcggtc ctggttgtgg
     6181 cgaaggttga gaagggcaag tccaagaagc tcaagagcgt gaaggagctg ctggggatca
     6241 cgattatgga gcgctccagc ttcgagaaga acccgatcga tttcctggag gcgaagggct
     6301 acaaggaggt gaagaaggac ctgatcatta agctccccaa gtactcactc ttcgagctgg
     6361 agaacggcag gaagcggatg ctggcttccg ctggcgagct gcagaagggg aacgagctgg
     6421 ctctgccgtc caagtatgtg aacttcctct acctggcctc ccactacgag aagctcaagg
     6481 gcagccccga ggacaacgag cagaagcagc tgttcgtcga gcagcacaag cattacctcg
     6541 acgagatcat tgagcagatt tccgagttct ccaagcgcgt gatcctggcc gacgcgaatc
     6601 tggataaggt cctctccgcg tacaacaagc accgcgacaa gccaatcagg gagcaggctg
     6661 agaatatcat tcatctcttc accctgacga acctcggcgc ccctgctgct ttcaagtact
     6721 tcgacacaac tatcgatcgc aagaggtaca caagcactaa ggaggtcctg gacgcgaccc
     6781 tcatccacca gtcgattacc ggcctctacg agacgcgcat cgacctgtct cagctcgggg
     6841 gcgacaagcg gccagcggcg acgaagaagg cggggcaggc gaagaagaag aagaagggag
     6901 acggctctgg atcggggtcg ggttctggct cagtcgacga tgctcttgac gattttgacc
     6961 tcgatatgct cgacgctctt gatgattttg atctcgacat gctcgatgca cttgatgact
     7021 ttgaccttga catgctcgac gcactcgatg acttcgacct cgacatgctt gagggcagag
     7081 gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatggcg tcaaatttca
     7141 cgcagtttgt tttggttgat aacggcggga ctggcgacgt tacagtagct ccatcaaatt
     7201 ttgcgaacgg agtcgctgag tggattagct caaattcaag gtcccaggcc tacaaggtta
     7261 cctgttctgt taggcagagt tctgcgcaaa aaagaaaata taccatcaag gttgaagtcc
     7321 ctaaagttgc aacacaaaca gtcggtggtg ttgagctccc tgtggcagcc tggagatctt
     7381 acttaaacat ggagctaaca attccaatat tcgctacaaa ctctgattgt gaactgattg
     7441 ttaaggcgat gcaaggcctc ttgaaagatg gaaaccctat accgtccgct atcgcagcta
     7501 acagcggtat ctatcctaag aagaagagga aggttggctc tggatcgggg tcgggttctg
     7561 gctcaggatc cggtacctat ccctatgacg tgcccgatta tgccagcctg ggcagcggct
     7621 cccccaagaa aaaacgcaag gtggaagatc ctaagaaaaa gcggaaagtg gacggcattg
     7681 gtagtgggag caacggcagc agcggatcca acggtccgac tgacgccgcg gaagaagaac
     7741 ttttgagcaa gaattatcat cttgagaacg aagtggctcg tcttaagaaa ggttctggca
     7801 gtggagaaga actgctttca aagaattacc acctggaaaa tgaggtagct agactgaaaa
     7861 aggggagcgg aagtggggag gagttgctga gcaaaaatta tcatttggag aacgaagtag
     7921 cacgactaaa gaaagggtcc ggatcgggtg aggagttact ctcgaaaaat tatcatctcg
     7981 aaaacgaagt ggctcggcta aaaaagggca gtggttctgg agaagagcta ttatctaaaa
     8041 actaccacct cgaaaatgag gtggcacgct taaaaaaggg aagtggcagt ggtgaagagc
     8101 tactatccaa gaattatcat cttgagaacg aggtagcgcg tttgaagaag ggttccggct
     8161 caggagagga actgctctcg aagaactatc atcttgaaaa tgaggtcgct cgattaaaaa
     8221 agggatcggg cagtggtgag gaactacttt caaagaatta ccacctcgaa aacgaagtag
     8281 ctcgattaaa gaaaggttca gggtcgggtg aagaattact gagtaaaaat tatcatctgg
     8341 aaaatgaggt agcgagacta aaaaagggga gtggttctgg cgaggaattg ctatcgaaaa
     8401 attatcatct tgagaacgaa gttgctaggc tcaaaaaggg ctcaggctca ggcaccgcgg
     8461 taaacatagg tggtggaacc ggtccgatgg atctacagcg gccgcaaggt ggaggtggac
     8521 ccaagaagaa gcgcaaggtg tgaactagtt aaagagcttt cgttcgtatc atcggtttcg
     8581 acaacgttcg tcaagttcaa tgcatcagtt tcattgcgca cacaccagaa tcctactgag
     8641 tttgagtatt atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt
     8701 tactgtgttt tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga
     8761 gttaatgaat gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct
     8821 tatttgttgt gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa
     8881 aaatgtgtca aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacacttgta
     8941 gttgtaccat tatgcttatt cactaggcaa caaatatatt ttcagaccta gaaaagctgc
     9001 aaatgttact gaatacaagt atgtcctctt gtgttttaga catttatgaa ctttccttta
     9061 tgtaattttc cagaatcctt gtcagattct aatcattgct ttataattat agttatactc
     9121 atggatttgt agttgagtat gaaaatattt tttaatgcat tttatgactt gcccgtacgc
     9181 tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
     9241 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
     9301 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
     9361 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
     9421 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
     9481 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
     9541 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
     9601 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
     9661 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
     9721 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
     9781 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
     9841 ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga
     9901 cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg
     9961 gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc tttcccaccg
    10021 ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt
    10081 tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac
    10141 ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccc cctctctacc
    10201 ttctctagat cggcgttccg gtccatggtt agggcccggt agttctactt ctgttcatgt
    10261 ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca cggatgcgac
    10321 ctgtacgtca gacacgttct gattgctaac ttgccagtgt ttctctttgg ggaatcctgg
    10381 gatggctcta gccgttccgc agacgggatc gatttcatga ttttttttgt ttcgttgcat
    10441 agggtttggt ttgccctttt cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc
    10501 atcttttcat gctttttttt gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc
    10561 tagatcggag tagaattaat tctgtttcaa actacctggt ggatttatta attttggatc
    10621 tgtatgtgtg tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg
    10681 atctaggata ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt
    10741 tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg
    10801 agtagaatac tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg
    10861 tcatacatct tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat
    10921 acatgttgat gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat
    10981 atgctctaac cttgagtacc tatctattat aataaacaag tatgttttat aattattttg
    11041 atcttgatat acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg
    11101 ccttcatacg ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt
    11161 tggtgttact tctgcagggc gccatggggc cggacatagt catgacacaa agtccgagtt
    11221 cgctttctgc gagtgttggg gatagagtta cgataacctg cagatctagt acgggggccg
    11281 tcaccacgtc gaattacgcg tcatgggtcc aagaaaagcc aggcaaactg tttaagggtc
    11341 ttataggtgg cacgaacaac cgggctcccg gagtcccttc caggtttagt ggatcactta
    11401 ttggtgacaa ggcaacgctg acgatatctt ccctccagcc agaagatttc gcgacctact
    11461 tttgtgcact ttggtattct aaccactggg tctttggcca agggacgaag gtcgagctca
    11521 aacggggtgg cggtgggagc ggcgggggrg gatcaggggg sggaggttcg agtggcgggg
    11581 ggagcgaagt caaactcctc gagtccggtg gaggattggt gcaaccaggt ggttcattga
    11641 agctctcgtg tgcagtttct ggtttctctt tgacagacta cggtgtcaat tgggtccggc
    11701 aggcaccggg gcgcggtctt gagtggatcg gcgttatctg gggcgacgga ataacagatt
    11761 ataactctgc tttgaaggac agattcatca ttagcaagga caacgggaag aacacggtgt
    11821 acctccagat gagcaaagtg cgcagtgatg atactgccct gtattactgc gtcacaggtt
    11881 tgtttgatta ctggggtcaa ggcactcttg tgacagtctc ttcgtatcct tatgacgtgc
    11941 ccgactacgc tggcggaggg ggcggcagcg gaggcggtgg atccggaggc ggtggctcag
    12001 ggggcggcgg gtcgcttgat cctggtggcg gaggttctgg gagcaaagga gaggagctct
    12061 ttacaggtgt cgtccctatt ttggtggagc ttgatggaga tgtcaatggc cataaatttt
    12121 ccgtcagggg cgaaggggag ggggacgcga ctaatggaaa attgaccctc aagtttatct
    12181 gcacgacagg aaagttgccg gtcccctggc ccactctggt taccaccctc acttatgggg
    12241 tccagtgctt tagcagatac cctgatcata tgaaacgcca tgacttcttc aaatcagcca
    12301 tgccagaggg ttatgtgcag gaaagaacta taagctttaa ggacgatggg acgtacaaaa
    12361 cgcgggcaga ggttaagttt gagggggata ctcttgtgaa taggatcgaa cttaagggca
    12421 tcgattttaa agaagacggt aatattcttg gccacaagtt ggagtataat ttcaattctc
    12481 acaacgtgta cataacagca gataaacaga aaaatgggat caaggccaat tttaaaatta
    12541 ggcataatgt tgaagatggg tctgtgcaac tggcagatca ttatcagcag aatactccaa
    12601 ttggcgatgg ccctgtcttg ttgcctgaca atcattacct ttccacgcag tcagtcctgt
    12661 ccaaagatcc gaatgagaag cgggaccaca tggtcttgct cgaattcgtt actgccgccg
    12721 gaatcactca tggcatggac gagctttaca agggaggtgg aaggactgga ggtggcggag
    12781 gggggctgct tgaccccgga acccctatgg atgccgactt ggtggcctcc tccacggttg
    12841 tttgggaaca ggatgccgac ccttttgcgg gcactgctga cgattttccc gctttcaatg
    12901 aggaggagtt ggcttggctg atggaacttc tcccacaggg tggtagtggt ggcctcctgg
    12961 atccggggac accgatggac gcagacctgg tggcgtcgtc aacggttgtg tgggaacagg
    13021 atgctgatcc gttcgcagga actgccgacg attttcctgc attcaacgaa gaggaactcg
    13081 catggctcat ggagcttctg cctcagggat cgggtggcgg gtcgcgaacg gaagaataca
    13141 agcttatatt gaacggtaag acactgaaag gtgaaactac gacagaagcc gttgacgcgg
    13201 caactgcgga gaaagtcttt aagcagtatg ctaacgataa tggcgtggac ggggagtgga
    13261 cgtatgacga cgcaaccaaa acattcacgg tcaccgaggg gggcggcagc ggaggcggta
    13321 cctcgcccaa aacccggaga cgccctaggc gctcgcagcg caaaagacct cccacgtaaa
    13381 gagctttcgt tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc atcagtttca
    13441 ttgcgcacac accagaatcc tactgagttt gagtattatg gcattgggaa aactgttttt
    13501 cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt attcggtttt cgctatcgaa
    13561 ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat atggtccttt tgttcattct
    13621 caaattaata ttatttgttt tttctcttat ttgttgtgtg ttgaatttga aattataaga
    13681 gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa
    13741 gttaatatga ggagtaaaac acttgtagtt gtaccattat gcttattcac taggcaacaa
    13801 atatattttc agacctagaa aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg
    13861 ttttagacat ttatgaactt tcctttatgt aattttccag aatccttgtc agattctaat
    13921 cattgcttta taattatagt tatactcatg gatttgtagt tgagtatgaa aatatttttt
    13981 aatgcatttt atgacttgcc aattgttccg gaactctaga taagcttacc ggaaagggcg
    14041 aattcgcaac tttgtataca aaagttgccc catggcgttc cctctagata acgcaggatc
    14101 ctatttactt taaatttttc ttatggctca gcctgtgatg gataactgaa tcaaacaaat
    14161 ggcgtctggg tttaagaaca tctgttttgg ctatgttgga cgaaacaagt gaacttttag
    14221 gatcaacttc cgtttatata cggagcttat atcgagcaat aagataagtg ggctttttat
    14281 gtaatttaat gggctatcgt ccatatattc actaataccc atgcccagta cccatgtatg
    14341 cgtttcatat aagctcctaa tttctcccac atcgctcaaa tctaaacaaa tcttgttgta
    14401 tatataacac tgagggagca ccattggtca gagaccggcg ccgctacagg gcgcgtccca
    14461 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    14521 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    14581 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata
    14641 gggcgaattg ggtaccgggc cccccctcga ggtcctccag cttttgttcc ctttagtgag
    14701 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    14761 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    14821 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc caccggtggt
    14881 ctctgtttta gagctaggcc aacatgagga tcacccatgt ctgcagggcc tagcaagtta
    14941 aaataaggct agtccgttat caacttggcc aacatgagga tcacccatgt ctgcagggcc
    15001 aagtggcacc gagtcggtgc ttttttttcc ctttcctttt ttcttttttt tgccataaac
    15061 ttaaatttgt atatcgatca ttgtagatat tgaaaaccta gaacaaacca acatccatgt
    15121 gaatgtcttt catgactgat ttagagataa ttcttgaatt ttggaactag aatctataat
    15181 gagcctaaat taaaacattg tgaactagta agggcgaatt cgacccagct ttcttgtaca
    15241 aagtggttcg ataattctta attaactagt tctagagcgg ccgccaccgc ggtggagctc
    15301 gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    15361 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    15421 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    15481 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc
    15541 ttgccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
    15601 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
    15661 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
    15721 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
    15781 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
    15841 ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac tctcgtctac
    15901 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
    15961 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
    16021 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
    16081 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
    16141 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
    16201 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
    16261 taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat
    16321 ctatctctct cgagtctacc atgagcccag aacgacgccc ggccgacatc cgccgtgcca
    16381 ccgaggcgga catgccggcg gtctgcacca tcgtcaacca ctacatcgag acaagcacgg
    16441 tcaacttccg taccgagccg caggaaccgc aggagtggac ggacgacctc gtccgtctgc
    16501 gggagcgcta tccctggctc gtcgccgagg tggacggcga ggtcgccggc atcgcctacg
    16561 cgggcccctg gaaggcacgc aacgcctacg actggacggc cgagtcgacc gtgtacgtct
    16621 ccccccgcca ccagcggacg ggactgggct ccacgctcta cacccacctg ctgaagtccc
    16681 tggaggcaca gggcttcaag agcgtggtcg ctgtcatcgg gctgcccaac gacccgagcg
    16741 tgcgcatgca cgaggcgctc ggatatgccc cccgcggcat gctgcgggcg gccggcttca
    16801 agcacgggaa ctggcatgac gtgggtttct ggcagctgga cttcagcctg ccggtaccgc
    16861 cccgtccggt cctgcccgtc accgagattt gactcgagtt tctccataat aatgtgtgag
    16921 tagttcccag ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata
    16981 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
    17041 ctaaaaccaa aatccagtac taaaatccag atcccccgaa ttaattcggc gttaattcag
    17101 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
    17161 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
    17221 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
    17281 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
    17341 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
    17401 ctgcctgtga tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact
    17461 ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca
    17521 gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa
    17581 aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat
    17641 cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct
    17701 ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac
    17761 ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc
    17821 aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga
    17881 tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct
    17941 gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa
    18001 tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt
    18061 ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt
    18121 tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag
    18181 caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag
    18241 ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga
    18301 agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat
    18361 aaaatattat attttactgg atgaattgtt ttagtaccta gaatgcatga ccaaaatccc
    18421 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    18481 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    18541 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    18601 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
    18661 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    18721 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    18781 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    18841 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    18901 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    18961 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    19021 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    19081 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    19141 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    19201 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
    19261 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
    19321 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
    19381 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
    19441 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
    19501 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
    19561 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
    19621 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
    19681 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
    19741 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
    19801 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
    19861 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
    19921 cgtgctatcc acaggaaaca gaccttttcg acctttttcc cctgctaggg caatttgccc
    19981 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
    20041 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
    20101 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
    20161 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
    20221 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
    20281 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
    20341 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
    20401 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
    20461 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
    20521 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
    20581 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
    20641 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
    20701 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
    20761 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
    20821 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
    20881 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
    20941 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
    21001 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
    21061 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
    21121 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
    21181 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
    21241 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
    21301 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
    21361 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
    21421 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
    21481 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
    21541 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
    21601 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
    21661 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
    21721 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
    21781 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
    21841 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
    21901 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
    21961 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
    22021 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
    22081 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
    22141 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
    22201 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
    22261 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
    22321 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
    22381 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
    22441 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
    22501 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
    22561 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
    22621 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
    22681 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
    22741 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
    22801 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
    22861 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
    22921 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
    22981 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
    23041 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
    23101 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtatt
//
