LOCUS       sequence-454165-       19101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot 
            plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA 
            was driven by OsU3 promoter; Hygromycin for plants selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19101)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 19101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT34
FEATURES             Location/Qualifiers
     source          1..19101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     164..184
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        173..219
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             294..1319
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    1360..1534
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    1612..1636
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3260..4054
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4141..4729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4630..4649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4883..4900
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4915..5055
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4981..5000)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5141..5163
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5399..5593
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5659..6732)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7161..7790)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9085..9109
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     9312..9328
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9344..11341
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    11384..11408
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11448..11468
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12069..12134
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             12141..12161
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(12186..12209,12210..12212,12213..16286)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             16199..16207
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             16287..16334
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    17009..17029
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        17066..17443
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             complement(17414..17746)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLCHG
                     SSAQLF"
     primer_bind     17611..17627
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17637..17656
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        17637..17655
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(17703..17723)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(17703..17721)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(17742..17758)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    17766..17782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(17790..17796,17797..17814,17815..17820))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17835..17856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        17898..17973
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(18062..18086)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      18150..18402
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(18419..18435)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    18443..18459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(18467..18473,18474..18491,18492..18497))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18512..18533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
       61 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
      121 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
      181 cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaaat caccagtctc
      241 tctctacaaa tctatctctc tcgagctttc gcagatccgg ggggcaatga gatatgaaaa
      301 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
      361 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
      421 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
      481 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggagt
      541 ttagcgagag cctgacctat tgcatctccc gccgttcaca gggtgtcacg ttgcaagacc
      601 tgcctgaaac cgaactgccc gctgttctac aaccggtcgc ggaggctatg gatgcgatcg
      661 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
      721 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
      781 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
      841 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
      901 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
      961 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     1021 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccacgactcc
     1081 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     1141 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     1201 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     1261 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aagaaataga
     1321 gtagatgccg accgggatct gtcgatcgac aagctcgagt ttctccataa taatgtgtga
     1381 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
     1441 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     1501 cctaaaacca aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca
     1561 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     1621 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     1681 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     1741 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     1801 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     1861 gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac
     1921 tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac
     1981 agtgaattgg agttcgtctt gttataatta gggaaggtgc gaacaagtcc ctgatatgag
     2041 atcatgtttg tcatctggag ccatagaaca gggttcatca tgagtcatca acttaccttc
     2101 gccgacagtg aattcagcag taagcgccgt cagaccagaa aagagatttt cttgtcccgc
     2161 atggagcaga ttctgccatg gcaaaacatg gtggaagtca tcgagccgtt ttaccccaag
     2221 gctggtaatg gccggcgacc ttatccgctg gaaaccatgc tacgcattca ctgcatgcag
     2281 cattggtaca acctgagcga tggcgcgatg gaagatgctc tgtacgaaat cgcctccatg
     2341 cgtctgtttg cccggttatc cctggatagc gccttgccgg accgcaccac catcatgaat
     2401 ttccgccacc tgctggagca gcatcaactg gcccgccaat tgttcaagac catcaatcgc
     2461 tggctggccg aagcaggcgt catgatgact caaggcacct tggtcgatgc caccatcatt
     2521 gaggcaccca gctcgaccaa gaacaaagag cagcaacgcg atccggagat gcatcagacc
     2581 aagaaaggca atcagtggca ctttggcatg aaggcccaca ttggtgtcga tgccaagagt
     2641 ggcctgaccc acagcctggt caccaccgcg gccaacgagc atgacctcaa tcagctgggt
     2701 aatctgctgc atggagagga gcaatttgtc tcagccgatg ccggctacca aggggcgcca
     2761 cagcgcgagg agctggccga ggtggatgtg gactggctga tcgccgagcg ccccggcaag
     2821 gtaagaacct tgaaacagca tccacgcaag aacaaaacgg ccatcaacat cgaatacatg
     2881 aaagccagca tccgggccag ggtggagcac ccatttcgca tcatcaagcg acagttcggc
     2941 ttcgtgaaag ccagatacaa ggggttgctg aaaaacgata accaactggc gatgttattc
     3001 acgctggcca acctgtttcg ggcggaccaa atgatacgtc agtgggagag atctcactaa
     3061 aaactgggga taacgcctta aatggcgaag aaacggtcta aataggctga ttcaaggcat
     3121 ttacgggaga aaaaatcggc tcaaacatga agaaatgaaa tgactgagtc agccgagaag
     3181 aatttccccg cttattcgca ccttccttag cttcttgggg tatctttaaa tactgtagaa
     3241 aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat
     3301 cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct
     3361 ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac
     3421 ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc
     3481 aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga
     3541 tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct
     3601 gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa
     3661 tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt
     3721 ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt
     3781 tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag
     3841 caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag
     3901 ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga
     3961 agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat
     4021 aaaatattat attttactgg atgaattgtt ttagtaccta gaatgcatga ccaaaatccc
     4081 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4141 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4201 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4261 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4321 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4381 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4441 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4501 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4561 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4621 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4681 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4741 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4801 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4861 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     4921 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     4981 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     5041 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     5101 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     5161 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     5221 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     5281 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     5341 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     5401 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     5461 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     5521 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     5581 cgtgctatcc acaggaaaca gaccttttcg acctttttcc cctgctaggg caatttgccc
     5641 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     5701 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     5761 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     5821 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     5881 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     5941 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     6001 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     6061 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     6121 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     6181 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     6241 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     6301 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     6361 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     6421 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     6481 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     6541 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     6601 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     6661 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     6721 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     6781 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     6841 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     6901 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     6961 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     7021 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     7081 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     7141 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     7201 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     7261 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     7321 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     7381 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     7441 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     7501 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     7561 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     7621 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     7681 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     7741 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     7801 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     7861 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     7921 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     7981 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     8041 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     8101 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctaggc
     8161 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
     8221 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
     8281 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
     8341 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
     8401 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
     8461 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
     8521 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
     8581 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
     8641 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
     8701 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
     8761 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
     8821 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
     8881 cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact
     8941 atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca
     9001 tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt
     9061 ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag
     9121 tttaaactga aggcgggaaa cgacaatctg atccaagctc aagctgctct agcattcgcc
     9181 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     9241 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     9301 gtcacgacgt tgtaaaacga cggccagtgc caagcttgca tgcctgcagt gcagcgtgac
     9361 ccggtcgtgc ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc
     9421 acatattttt tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt
     9481 aaactttact ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga
     9541 atcatataaa tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga
     9601 ctctacagtt ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca
     9661 cctatataat acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt
     9721 tttatagact aattttttta gtacatctat tttattctat tttagcctct aaattaagaa
     9781 aactaaaact ctattttagt ttttttattt aataatttag atataaaata gaataaaata
     9841 aagtgactaa aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt
     9901 tcttgtttcg agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc
     9961 aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt
    10021 cgctgcctct ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg
    10081 catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc
    10141 tcctctcacg gcaccggcag ctacggggga ttcctttccc accgctcctt cgctttccct
    10201 tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt
    10261 gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc
    10321 ttcaaggtac gccgctcgtc cccccccccc cccccctctc taccttctct agatcggcgt
    10381 tccggtccat ggttagggcc cggtagttct acttctgttc atgtttgtgt tagatccgtg
    10441 tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac gtcagacacg
    10501 ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc tctagccgtt
    10561 ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt tggtttgccc
    10621 ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt
    10681 ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc ggagtagaat
    10741 taattctgtt tcaaactacc tggtggattt attaattttg gatctgtatg tgtgtgccat
    10801 acatattcat agttacgaat tgaagatgat ggatggaaat atcgatctag gataggtata
    10861 catgttgatg cgggttttac tgatgcatat acagagatgc tttttgttcg cttggttgtg
    10921 atgatgtggt gtggttgggc ggtcgttcat tcgttctaga tcggagtaga atactgtttc
    10981 aaactacctg gtgtatttat taattttgga actgtatgtg tgtgtcatac atcttcatag
    11041 ttacgagttt aagatggatg gaaatatcga tctaggatag gtatacatgt tgatgtgggt
    11101 tttactgatg catatacatg atggcatatg cagcatctat tcatatgctc taaccttgag
    11161 tacctatcta ttataataaa caagtatgtt ttataattat tttgatcttg atatacttgg
    11221 atgatggcat atgcagcagc tatatgtgga tttttttagc cctgccttca tacgctattt
    11281 atttgcttgg tactgtttct tttgtcgatg ctcaccctgt tgtttggtgt tacttctgca
    11341 ggtcgactct agaggatccc ctcgaggcgc gccaagctat caaacaagtt tgtacaaaaa
    11401 agcaggctcc gatgaaacgg acagccgacg gaagcgagtt cgagtcacca aagaagaagc
    11461 ggaaagtctc tgaggtggag ttttcccacg agtactggat gagacatgcc ctgaccctgg
    11521 ccaagagggc acgggatgag agggaggtgc ctgtgggagc cgtgctggtg ctgaacaata
    11581 gagtgatcgg cgagggctgg aacagagcca tcggcctgca cgacccaaca gcccatgccg
    11641 aaattatggc cctgagacag ggcggcctgg tcatgcagaa ctacagactg attgacgcca
    11701 ccctgtacgt gacattcgag ccttgcgtga tgtgcgccgg cgccatgatc cactctagga
    11761 tcggccgcgt ggtgtttggc gtgaggaact caaaaagagg cgccgcaggc tccctgatga
    11821 acgtgctgaa ctaccccggc atgaatcacc gcgtcgaaat taccgaggga atcctggcag
    11881 atgaatgtgc cgccctgctg tgcgatttct atcggatgcc tagacaggtg ttcaatgctc
    11941 agaagaaggc ccagagctcc atcaactccg gaggatctag cggaggctcc tctggctctg
    12001 agacacctgg cacaagcgag agcgcaacac ctgaaagcag cgggggcagc agcggggggt
    12061 caaccatgga ttacaaggac cacgacgggg attacaagga ccacgacatt gattacaagg
    12121 atgatgatga caagatggct ccgaagaaga agaggaaggt tggcatccac ggggtgccag
    12181 ctgctgacaa gaagtactcg atcggcctcg ctattgggac taactctgtt ggctgggccg
    12241 tgatcaccga cgagtacaag gtgccctcaa agaagttcaa ggtcctgggc aacaccgatc
    12301 ggcattccat caagaagaat ctcattggcg ctctcctgtt cgacagcggc gagacggctg
    12361 aggctacgcg gctcaagcgc accgcccgca ggcggtacac gcgcaggaag aatcgcatct
    12421 gctacctgca ggagattttc tccaacgaga tggcgaaggt tgacgattct ttcttccaca
    12481 ggctggagga gtcattcctc gtggaggagg ataagaagca cgagcggcat ccaatcttcg
    12541 gcaacattgt cgacgaggtt gcctaccacg agaagtaccc tacgatctac catctgcgga
    12601 agaagctcgt ggactccaca gataaggcgg acctccgcct gatctacctc gctctggccc
    12661 acatgattaa gttcaggggc catttcctga tcgaggggga tctcaacccg gacaatagcg
    12721 atgttgacaa gctgttcatc cagctcgtgc agacgtacaa ccagctcttc gaggagaacc
    12781 ccattaatgc gtcaggcgtc gacgcgaagg ctatcctgtc cgctaggctc tcgaagtctc
    12841 ggcgcctcga gaacctgatc gcccagctgc cgggcgagaa gaagaacggc ctgttcggga
    12901 atctcattgc gctcagcctg gggctcacgc ccaacttcaa gtcgaatttc gatctcgctg
    12961 aggacgccaa gctgcagctc tccaaggaca catacgacga tgacctggat aacctcctgg
    13021 cccagatcgg cgatcagtac gcggacctgt tcctcgctgc caagaatctg tcggacgcca
    13081 tcctcctgtc tgatattctc agggtgaaca ccgagattac gaaggctccg ctctcagcct
    13141 ccatgatcaa gcgctacgac gagcaccatc aggatctgac cctcctgaag gcgctggtca
    13201 ggcagcagct ccccgagaag tacaaggaga tcttcttcga tcagtcgaag aacggctacg
    13261 ctgggtacat tgacggcggg gcctctcagg aggagttcta caagttcatc aagccgattc
    13321 tggagaagat ggacggcacg gaggagctgc tggtgaagct caatcgcgag gacctcctga
    13381 ggaagcagcg gacattcgat aacggcagca tcccacacca gattcatctc ggggagctgc
    13441 acgctatcct gaggaggcag gaggacttct accctttcct caaggataac cgcgagaaga
    13501 tcgagaagat tctgactttc aggatcccgt actacgtcgg cccactcgct aggggcaact
    13561 cccgcttcgc ttggatgacc cgcaagtcag aggagacgat cacgccgtgg aacttcgagg
    13621 aggtggtcga caagggcgct agcgctcagt cgttcatcga gaggatgacg aatttcgaca
    13681 agaacctgcc aaatgagaag gtgctcccta agcactcgct cctgtacgag tacttcacag
    13741 tctacaacga gctgactaag gtgaagtatg tgaccgaggg catgaggaag ccggctttcc
    13801 tgtctgggga gcagaagaag gccatcgtgg acctcctgtt caagaccaac cggaaggtca
    13861 cggttaagca gctcaaggag gactacttca agaagattga gtgcttcgat tcggtcgaga
    13921 tctctggcgt tgaggaccgc ttcaacgcct ccctggggac ctaccacgat ctcctgaaga
    13981 tcattaagga taaggacttc ctggacaacg aggagaatga ggatatcctc gaggacattg
    14041 tgctgacact cactctgttc gaggaccggg agatgatcga ggagcgcctg aagacttacg
    14101 cccatctctt cgatgacaag gtcatgaagc agctcaagag gaggaggtac accggctggg
    14161 ggaggctgag caggaagctc atcaacggca ttcgggacaa gcagtccggg aagacgatcc
    14221 tcgacttcct gaagagcgat ggcttcgcga accgcaattt catgcagctg attcacgatg
    14281 acagcctcac attcaaggag gatatccaga aggctcaggt gagcggccag ggggactcgc
    14341 tgcacgagca tatcgcgaac ctcgctggct cgccagctat caagaagggg attctgcaga
    14401 ccgtgaaggt tgtggacgag ctggtgaagg tcatgggcag gcacaagcct gagaacatcg
    14461 tcattgagat ggcccgggag aatcagacca cgcagaaggg ccagaagaac tcacgcgaga
    14521 ggatgaagag gatcgaggag ggcattaagg agctggggtc ccagatcctc aaggagcacc
    14581 cggtggagaa cacgcagctg cagaatgaga agctctacct gtactacctc cagaatggcc
    14641 gcgatatgta tgtggaccag gagctggata ttaacaggct cagcgattac gacgtcgatc
    14701 atatcgttcc acagtcattc ctgaaggatg actccattga caacaaggtc ctcaccaggt
    14761 cggacaagaa ccggggcaag tctgataatg ttccttcaga ggaggtcgtt aagaagatga
    14821 agaactactg gcgccagctc ctgaatgcca agctgatcac gcagcggaag ttcgataacc
    14881 tcacaaaggc tgagaggggc gggctctctg agctggacaa ggcgggcttc atcaagaggc
    14941 agctggtcga gacacggcag atcactaagc acgttgcgca gattctcgac tcacggatga
    15001 acactaagta cgatgagaat gacaagctga tccgcgaggt gaaggtcatc accctgaagt
    15061 caaagctcgt ctccgacttc aggaaggatt tccagttcta caaggttcgg gagatcaaca
    15121 attaccacca tgcccatgac gcgtacctga acgcggtggt cggcacagct ctgatcaaga
    15181 agtacccaaa gctcgagagc gagttcgtgt acggggacta caaggtttac gatgtgagga
    15241 agatgatcgc caagtcggag caggagattg gcaaggctac cgccaagtac ttcttctact
    15301 ctaacattat gaatttcttc aagacagaga tcactctggc caatggcgag atccggaagc
    15361 gccccctcat cgagacgaac ggcgagacgg gggagatcgt gtgggacaag ggcagggatt
    15421 tcgcgaccgt caggaaggtt ctctccatgc cacaagtgaa tatcgtcaag aagacagagg
    15481 tccagactgg cgggttctct aaggagtcaa ttctgcctaa gcggaacagc gacaagctca
    15541 tcgcccgcaa gaaggactgg gatccgaaga agtacggcgg gttcgacagc cccactgtgg
    15601 cctactcggt cctggttgtg gcgaaggttg agaagggcaa gtccaagaag ctcaagagcg
    15661 tgaaggagct gctggggatc acgattatgg agcgctccag cttcgagaag aacccgatcg
    15721 atttcctgga ggcgaagggc tacaaggagg tgaagaagga cctgatcatt aagctcccca
    15781 agtactcact cttcgagctg gagaacggca ggaagcggat gctggcttcc gctggcgagc
    15841 tgcagaaggg gaacgagctg gctctgccgt ccaagtatgt gaacttcctc tacctggcct
    15901 cccactacga gaagctcaag ggcagccccg aggacaacga gcagaagcag ctgttcgtcg
    15961 agcagcacaa gcattacctc gacgagatca ttgagcagat ttccgagttc tccaagcgcg
    16021 tgatcctggc cgacgcgaat ctggataagg tcctctccgc gtacaacaag caccgcgaca
    16081 agccaatcag ggagcaggct gagaatatca ttcatctctt caccctgacg aacctcggcg
    16141 cccctgctgc tttcaagtac ttcgacacaa ctatcgatcg caagaggtac acaagcacta
    16201 aggaggtcct ggacgcgacc ctcatccacc agtcgattac cggcctctac gagacgcgca
    16261 tcgacctgtc tcagctcggg ggcgacaagc ggccagcggc gacgaagaag gcggggcagg
    16321 cgaagaagaa gaagtgagct cagagctttc gttcgtatca tcggtttcga caacgttcgt
    16381 caagttcaat gcatcagttt cattgcgcac acaccagaat cctactgagt ttgagtatta
    16441 tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt actgtgtttt
    16501 ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag ttaatgaatg
    16561 atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt atttgttgtg
    16621 tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa aatgtgtcaa
    16681 atcgtggcct ctaatgaccg aagttaatat gaggagtaaa acacttgtag ttgtaccatt
    16741 atgcttattc actaggcaac aaatatattt tcagacctag aaaagctgca aatgttactg
    16801 aatacaagta tgtcctcttg tgttttagac atttatgaac tttcctttat gtaattttcc
    16861 agaatccttg tcagattcta atcattgctt tataattata gttatactca tggatttgta
    16921 gttgagtatg aaaatatttt ttaatgcatt ttatgacttg ccaattgttc cggaactcta
    16981 gataagctta ccggaaaggg cgaattcgca actttgtata caaaagttgc cccatggcgt
    17041 tccctctaga taacgcagga tcctaaaggg atctttaaac atacgaacag atcacttaaa
    17101 gttcttctga agcaacttaa agttatcagg catgcatgga tcttggagga atcagatgtg
    17161 cagtcaggga ccatagcaca ggacaggcgt cttctactgg tgctaccagc aaatgctgga
    17221 agccgggaac actgggtacg ttggaaacca cgtgatgtgg agtaagataa actgtaggag
    17281 aaaagcattt cgtagtgggc catgaagcct ttcaggacat gtattgcagt atgggccggc
    17341 ccattacgca attggacgac aacaaagact agtattagta ccacctcggc tatccacata
    17401 gatcaaagct ggtttaaaag agttgtgcag atgatccgtg gcagagaccg gcgccgctac
    17461 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    17521 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    17581 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
    17641 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcctc cagcttttgt
    17701 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
    17761 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    17821 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    17881 ttccaccggt ggtctctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
    17941 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt gtcccttcga agggcaattc
    18001 tgcagatatc catcacactg gcggccgctc gaggtcgagg gtatcgataa gcttgaattc
    18061 gacccagctt tcttgtacaa agtggttcga taattcttaa ttaactagtt ctagagcggc
    18121 cgccaccgcg gtggagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct
    18181 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
    18241 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
    18301 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
    18361 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattc gtaatcatgt
    18421 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    18481 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    18541 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    18601 gccaacgcgc ggggagaggc ggtttgcgta ttggctagag cagcttgcca acatggtgga
    18661 gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc
    18721 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
    18781 tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca
    18841 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg
    18901 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
    18961 agtggattga tgtgaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
    19021 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa
    19081 acctcctcgg attccattgc c
//
