LOCUS       sequence-454179-       17935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for dLbCas12a-D156R mediated A to G base editing for 
            monocot plants; TTTV PAM; LbCas12a-D156R-ABE and the crRNA were 
            driven by separate ZmUbi1 promoter; BASTA for plants selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17935)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 17935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT52
FEATURES             Location/Qualifiers
     source          1..17935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    283..307
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     510..526
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        542..2539
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    2582..2606
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2646..2666
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3294..3314
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7020..7067
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      7098..7350
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    7395..7415
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     ncRNA           9411..9447
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             complement(9457..9771)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLIYT
                     "
     primer_bind     9636..9652
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9662..9681
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9662..9680
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9728..9748)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9728..9746)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9767..9783)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9791..9807
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9815..9821,9822..9839,9840..9845))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9860..9881
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     ncRNA           9923..9990
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     protein_bind    complement(10010..10034)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(10102..10118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10126..10142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10150..10156,10157..10174,10175..10180))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10195..10216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10407..11083
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     11020..11040
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11128..11679
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    11686..11860
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11938..11962
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12387..13181
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13268..13856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13757..13776
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14010..14027
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14042..14182
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(14108..14127)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14268..14290
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14526..14720
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14786..15859)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16288..16917)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
       61 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
      121 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
      181 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
      241 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
      301 cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa
      361 gctgctctag cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
      421 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      481 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgcatg
      541 cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct
      601 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
      661 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
      721 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
      781 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
      841 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
      901 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
      961 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
     1021 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
     1081 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
     1141 acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag
     1201 acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg
     1261 gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca
     1321 cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt ccttycccmc
     1381 csctccttcg cttyccctyc cycgcccgcc gtaataaata gacaccccct ccacaccctc
     1441 tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc
     1501 mcccgtcggc acctccgctt caaggtacgc cgctcgtccy cccccccccc cccctctcta
     1561 ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat
     1621 gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg
     1681 acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct
     1741 gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc
     1801 atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg
     1861 tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt
     1921 tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat taattttgga
     1981 tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg atggaaatat
     2041 cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac agagatgctt
     2101 tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc gttctagatc
     2161 ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac tgtatgtgtg
     2221 tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc taggataggt
     2281 atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca gcatctattc
     2341 atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt ataattattt
     2401 tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt tttttagccc
     2461 tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct caccctgttg
     2521 tttggtgtta cttctgcagg tcgactctag aggatcccct cgaggcgcgc caagctatca
     2581 aacaagtttg tacaaaaaag caggctccga tgaaacggac agccgacgga agcgagttcg
     2641 agtcaccaaa gaagaagcgg aaagtctctg aggtggagtt ttcccacgag tactggatga
     2701 gacatgccct gaccctggcc aagagggcac gggatgagag ggaggtgcct gtgggagccg
     2761 tgctggtgct gaacaataga gtgatcggcg agggctggaa cagagccatc ggcctgcacg
     2821 acccaacagc ccatgccgaa attatggccc tgagacaggg cggcctggtc atgcagaact
     2881 acagactgat tgacgccacc ctgtacgtga cattcgagcc ttgcgtgatg tgcgccggcg
     2941 ccatgatcca ctctaggatc ggccgcgtgg tgtttggcgt gaggaactca aaaagaggcg
     3001 ccgcaggctc cctgatgaac gtgctgaact accccggcat gaatcaccgc gtcgaaatta
     3061 ccgagggaat cctggcagat gaatgtgccg ccctgctgtg cgatttctat cggatgccta
     3121 gacaggtgtt caatgctcag aagaaggccc agagctccat caacggaggg ggagggtcgg
     3181 gaggtggtgg cagcggagga ggcggtagcg gaggtggagg atcaggtggc ggagggtcgg
     3241 gagggggcgg ttctggtggt gggggctcgg gtggaggagg gagcaccatg gctcctaaga
     3301 agaagcggaa ggttggtatt cacggggtgc ctgcggcttc aaagctcgag aaattcacca
     3361 actgttattc gttgagcaaa acactgcggt ttaaagcgat tccagtcggc aagactcaag
     3421 agaatataga caataagcgg ctgttggtgg aagatgaaaa gcgcgcggaa gactacaaag
     3481 gggtgaagaa gttgttggac agatactacc tctcttttat caatgatgtc ttgcactcaa
     3541 tcaaattgaa gaatctgaac aactacatct ccctcttcag aaagaaaaca aggacagaaa
     3601 aggagaataa ggaacttgaa aatttggaga tcaatctgag gaaagagatc gcgaaagcct
     3661 ttaaaggcaa cgaaggatac aaaagtctgt tcaagaagga tataattgag acaattttgc
     3721 cagagttcct cgatgacaag gacgagattg cgctggtcaa ttcgttcaac ggattcacaa
     3781 cagcattcac aggcttcttt cgcaatcggg aaaatatgtt ctctgaggag gcaaagtcca
     3841 cttctattgc gttcaggtgt atcaatgaga atctcactag gtacatttcc aacatggata
     3901 tctttgagaa ggttgacgca atttttgaca agcacgaagt tcaggagatt aaggagaaga
     3961 tcctcaattc cgattatgac gttgaggact tcttcgaagg tgagtttttt aatttcgtgc
     4021 tcactcaaga gggtatcgac gtgtataatg cgatcatcgg tgggttcgtg actgagtccg
     4081 gtgaaaagat taagggattg aacgagtata tcaaccttta caaccaaaag acgaaacaga
     4141 agctgccaaa gttcaagcct ctttacaaac aggttctttc agaccgcgag tcactctcgt
     4201 tctatgggga gggctacact tcggatgagg aagtcctgga ggtgttcagg aatactctca
     4261 ataagaattc ggagattttc tcttctataa aaaaactgga aaagttgttt aagaattttg
     4321 acgaatactc tagcgccggc atatttgtga aaaacggccc ggccatatca acgataagta
     4381 aagatatctt cggcgaatgg aacgtgatca gagacaaatg gaacgcggag tatgacgata
     4441 ttcacctgaa gaagaaggct gtcgtaacgg agaagtacga ggatgatcgc aggaaaagct
     4501 tcaaaaagat cggaagtttc agcctggaac agttgcagga gtatgctgac gccgatctta
     4561 gcgtcgtcga gaagttgaag gagataatca tccaaaaggt cgacgagata tataaagtct
     4621 atggatcaag tgaaaaactg ttcgacgccg acttcgtttt ggagaagtcc ctgaagaaga
     4681 acgacgctgt tgttgccatt atgaaggatc tgctcgacag cgtgaagagt ttcgagaact
     4741 atattaaggc ttttttcggg gaggggaagg agactaacag agatgagtcc ttctacggag
     4801 acttcgtcct cgcgtacgat atactcctta aggtagacca catctacgac gcaatcagaa
     4861 attacgtgac acaaaagccg tacagcaagg acaagttcaa actctacttc cagaaccccc
     4921 agttcatggg cggctgggac aaggacaagg aaacggatta cagggctacg atcctgaggt
     4981 atggttcaaa atactacttg gcgattatgg acaagaagta cgccaagtgt ctccagaaga
     5041 ttgacaaaga cgatgtcaat ggcaattatg agaagatcaa ctacaagctg cttccgggtc
     5101 cgaacaagat gctcccaaag gttttcttca gcaagaaatg gatggcctac tataacccaa
     5161 gcgaggacat ccagaagatt tataagaacg gtacgttcaa gaagggcgac atgttcaatc
     5221 ttaacgactg tcacaagctg atcgacttct tcaaagactc aattagccgg tacccaaagt
     5281 ggtctaacgc ctatgacttc aacttttcgg aaaccgagaa gtacaaggat atagccggat
     5341 tttatagaga ggtggaagag cagggctaca aggtgtcatt cgagtccgcc agcaagaagg
     5401 aagtggacaa gctcgtggaa gagggtaagc tctacatgtt ccagatttat aataaagact
     5461 ttagcgataa gagccacggg acacctaatc tccacacaat gtatttcaag ctgctcttcg
     5521 acgagaataa ccacggccaa atcaggttgt caggaggggc tgaactcttc atgcggcgcg
     5581 ctagccttaa gaaggaggag cttgtagtcc accctgcgaa tagtccaatt gcgaataaga
     5641 acccggacaa tcctaaaaag actacaacat tgagctacga cgtgtacaag gataagaggt
     5701 tttccgagga tcagtacgag ctccacatcc cgattgcgat caacaagtgc ccaaagaata
     5761 ttttcaagat aaacacagag gtgcgtgtac tcctgaagca tgacgacaat ccttacgtca
     5821 ttgggattgc ccggggcgag aggaacctcc tctatattgt ggtggtggac gggaagggga
     5881 acatagtcga acagtactcc cttaacgaaa taattaacaa tttcaacggc atccgtatca
     5941 agaccgacta ccattcgttg ctggacaaga aggagaagga gagatttgag gcgcggcaaa
     6001 attggacaag tatcgagaac atcaaggaac tcaaagcagg ttatatctct caagttgtgc
     6061 ataagatatg cgagctggtt gagaagtatg acgcagtgat cgctcttgag gacctcaact
     6121 cgggctttaa gaattctaga gttaaagtgg agaagcaggt ctatcaaaag ttcgagaaga
     6181 tgcttataga taagctcaac tacatggtcg ataagaaatc gaacccatgt gccaccggcg
     6241 gcgcactcaa aggttaccaa ataacaaaca aattcgagtc cttcaaatcg atgagtactc
     6301 agaatgggtt catattttat ataccggcgt ggcttacgtc taagatcgac ccgtcaactg
     6361 gttttgtcaa cctgttgaag acgaaataca cgtccattgc cgattcgaaa aagttcatat
     6421 ctagttttga tcgtattatg tacgtcccag aggaagatct tttcgagttt gctctcgact
     6481 acaaaaactt ttcgcggacc gatgcggatt acattaaaaa atggaaactc tattcgtacg
     6541 gcaacagaat caggattttt cgcaacccta agaagaataa cgtctttgat tgggaggaag
     6601 tttgcttgac tagcgcgtac aaggagctct ttaataagta tggcattaac taccaacagg
     6661 gtgatatcag agcactgctt tgcgaacaat ctgacaaggc tttctactca tccttcatgg
     6721 ctttgatgag cctgatgctc cagatgagaa attcaattac aggcagaacc gacgtggatt
     6781 tcttgatctc cccggttaaa aattctgatg gcatctttta cgatagcagg aactatgaag
     6841 cgcaagagaa tgcgattctg ccaaaaaatg cagacgccaa cggtgcctat aacatcgcca
     6901 ggaaagtcct gtgggcgatc ggccagttca aaaaggccga agacgaaaaa ttggacaagg
     6961 tcaaaatcgc tatcagcaac aaagagtggc tggagtatgc tcagacatcc gtaaagcata
     7021 agcgtcctgc tgccaccaaa aaggccggac aggctaagaa aaagaagtga gacgactagt
     7081 ggcggccgcc gacgtccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     7141 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     7201 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     7261 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     7321 cgcgcgcggt gtcatctatg ttactagatc gggaattgat cccccctcga cagcttccgg
     7381 aaagggcgaa ttcgcaactt tgtatacaaa agttgcccca tggcgttccc tctagagaga
     7441 taatgagcat tgcatgtcta agttataaaa aattaccaca tatttttttt gtcacacttg
     7501 tttgaagtgc agtttatcta tctttataca tatatttaaa ctttactcta cgaataatat
     7561 aatctatagt actacaataa tatcagtgtt ttagagaatc atataaatga acagttagac
     7621 atggtctaaa ggacaattga gtattttgac aacaggactc tacagtttta tctttttagt
     7681 gtgcatgtgt tctccttttt ttttgcaaat agcttcacct atataatact tcatccattt
     7741 tattagtaca tccatttagg gtttagggtt aatggttttt atagactaat ttttttagta
     7801 catctatttt attctatttt agcctctaaa ttaagaaaac taaaactcta ttttagtttt
     7861 tttatttaat aatttagata taaaatagaa taaaataaag tgactaaaaa ttaaacaaat
     7921 accctttaag aaattaaaaa aactaaggaa acatttttct tgtttcgagt agataatgcc
     7981 agcctgttaa acgccgtcga cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc
     8041 gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc tgcctctgga cccctctcga
     8101 gagttccgct ccaccgttgg acttgctccg ctgtcggcat ccagaaattg cgtggcggag
     8161 cggcagacgt gagccggcac ggcaggcggc ctcctcctcc tctcacggca ccggcagcta
     8221 cgggggattc cttycccmcc gctccttcgc ttyccctycc ycgcccgccg taataaatag
     8281 acaccccctc cacaccctct ttccccaacc tcgtgttgtt cggagcgcac acacacacaa
     8341 ccagatctcc cccaaatccm cccgtcggcm cctccgcttc aaggtacgcc gctcgtccyc
     8401 cccccccccc ccctctctac cttctctaga tcggcgttcc ggtccatggt tagggcccgg
     8461 tagttctact tctgttcatg tttgtgttag atccgtgttt gtgttagatc cgtgctgcta
     8521 gcgttcgtac acggatgcga cctgtacgtc agacacgttc tgattgctaa cttgccagtg
     8581 tttctctttg gggaatcctg ggatggctct agccgttccg cagacgggat cgatttcatg
     8641 attttttttg tttcgttgca tagggtttgg tttgcccttt tcctttattt caatatatgc
     8701 cgtgcacttg tttgtcgggt catcttttca tgcttttttt tgtcttggtt gtgatgatgt
     8761 ggtctggttg ggcggtcgtt ctagatcgga gtagaattaa ttctgtttca aactacctgg
     8821 tggatttatt aattttggat ctgtatgtgt gtgccataca tattcatagt tacgaattga
     8881 agatgatgga tggaaatatc gatctaggat aggtatacat gttgatgcgg gttttactga
     8941 tgcatataca gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt
     9001 cgttcattcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg tatttattaa
     9061 ttttggaact gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag atggatggaa
     9121 atatcgatct aggataggta tacatgttga tgtgggtttt actgatgcat atacatgatg
     9181 gcatatgcag catctattca tatgctctaa ccttgagtac ctatctatta taataaacaa
     9241 gtatgtttta taattatttt gatcttgata tacttggatg atggcatatg cagcagctat
     9301 atgtggattt ttttagccct gccttcatac gctatttatt tgcttggtac tgtttctttt
     9361 gtcgatgctc accctgttgt ttggtgttac ttctgcaggg atccaaatta ctgatgagtc
     9421 cgtgaggacg aaacgagtaa gctcgtctaa tttctactaa gtgtagatga gaccggcgcc
     9481 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     9541 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     9601 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc
     9661 gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg tcctccagct
     9721 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     9781 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     9841 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     9901 ccgctttcca ccggtggtct ctggccggca tggtcccagc ctcctcgctg gcgccggctg
     9961 ggcaacatgc ttcggcatgg cgaatgggac ggtacccggc cggaattcga cccagctttc
    10021 ttgtacaaag tggttcgata attcttaatt aactagttct agagcggccg ccaccgcggt
    10081 ggagctcgaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
    10141 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
    10201 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    10261 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattggcta
    10321 gagcagcttg ccaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat
    10381 acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac
    10441 ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa
    10501 ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct
    10561 gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac
    10621 gttccaacca cgtcttcaaa gcaagtggat tgatgtgaac atggtggagc acgacactct
    10681 cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt
    10741 tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
    10801 catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg
    10861 aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
    10921 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
    10981 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
    11041 ctctatataa ggaagttcat ttcatttgga gaggacacgc tgaaatcacc agtctctctc
    11101 tacaaatcta tctctctcga gtctaccatg agcccagaac gacgcccggc cgacatccgc
    11161 cgtgccaccg aggcggacat gccggcggtc tgcaccatcg tcaaccacta catcgagaca
    11221 agcacggtca acttccgtac cgagccgcag gaaccgcagg agtggacgga cgacctcgtc
    11281 cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt cgccggcatc
    11341 gcctacgcgg gcccctggaa ggcacgcaac gcctacgact ggacggccga gtcgaccgtg
    11401 tacgtctccc cccgccacca gcggacggga ctgggctcca cgctctacac ccacctgctg
    11461 aagtccctgg aggcacaggg cttcaagagc gtggtcgctg tcatcgggct gcccaacgac
    11521 ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc gcggcatgct gcgggcggcc
    11581 ggcttcaagc acgggaactg gcatgacgtg ggtttctggc agctggactt cagcctgccg
    11641 gtaccgcccc gtccggtcct gcccgtcacc gagatttgac tcgagtttct ccataataat
    11701 gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga
    11761 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
    11821 ctaattccta aaaccaaaat ccagtactaa aatccagatc ccccgaatta attcggcgtt
    11881 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
    11941 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
    12001 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
    12061 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
    12121 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
    12181 agcgttgctg cctgtgatca ccgcggtttc aaaatcggct ccgtcgatac tatgttatac
    12241 gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca
    12301 aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac
    12361 tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg gaattgaaaa
    12421 aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat
    12481 ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag
    12541 ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc
    12601 ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg
    12661 aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta
    12721 tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct
    12781 atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg
    12841 ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg cgcgagctgt
    12901 atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg
    12961 gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa
    13021 gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc agggaggata
    13081 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
    13141 agaaaataaa atattatatt ttactggatg aattgtttta gtacctagaa tgcatgacca
    13201 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    13261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    13321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    13381 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
    13441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    13501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    13561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    13621 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    13681 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    13741 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    13801 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    13861 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    13921 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    13981 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    14041 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
    14101 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
    14161 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
    14221 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
    14281 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc
    14341 gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta
    14401 ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta
    14461 gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag
    14521 acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg
    14581 cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa
    14641 tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc
    14701 caatgtacgt gctatccaca ggaaacagac cttttcgacc tttttcccct gctagggcaa
    14761 tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt
    14821 tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact
    14881 ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc
    14941 cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg
    15001 cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat
    15061 cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat
    15121 cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc
    15181 ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg
    15241 tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt
    15301 tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga
    15361 acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat
    15421 gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg
    15481 cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt
    15541 cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca
    15601 cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa
    15661 tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg
    15721 cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg
    15781 cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg
    15841 atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg
    15901 ccggcaggca acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca
    15961 ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa
    16021 aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag
    16081 atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat
    16141 cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca
    16201 acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt
    16261 tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag
    16321 cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc
    16381 ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc
    16441 gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga
    16501 cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc
    16561 caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa
    16621 gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac
    16681 tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc
    16741 gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg
    16801 atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt
    16861 tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg
    16921 ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc
    16981 aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg
    17041 gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc
    17101 agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc
    17161 gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg
    17221 cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt
    17281 cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa
    17341 gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc
    17401 ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc
    17461 gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga
    17521 tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg
    17581 ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct
    17641 ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg
    17701 tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt
    17761 cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt
    17821 cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc
    17881 cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtatt
//
