LOCUS       sequence-454238-       13295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminally HA-tagged hBIG2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13295)
  TITLE     Mammalian expression plasmids and entry plasmids for Gateway
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG/HA-hBIG2
FEATURES             Location/Qualifiers
     source          1..13295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     618..637
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(856..960)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1272..2075
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1335..1354)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1945..1964
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2082..2130
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2140..2519
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2876..3892
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3816..3838
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3900..3919
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3988..4012
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4051..4077
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(11121..11145)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11189..11215
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(11303..11322)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11368..11423
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11422..11441)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(11784..11800)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11784..11800)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11797..11819)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11808..11824
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11832..11838,11839..11856,11857..11862))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11877..11898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11957..12152
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12003..12138
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    12158..12292
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12195..12214)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12249..12268
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12360..12377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12531..13119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12611..12630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13290..13295,1..786,787..855))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
       61 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
      121 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
      181 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
      241 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
      301 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
      361 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
      421 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
      481 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
      541 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
      601 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
      661 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
      721 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
      781 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
      841 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
      901 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
      961 cacatttccc cgaaaagtgc cacctgggtc gagcagtgtg gttttcaaga ggaagcaaaa
     1021 agcctctcca cccaggcctg gaatgtttcc acccaatgtc gagcagtgtg gttttgcaag
     1081 aggaagcaaa aagcctctcc acccaggcct ggaatgtttc cacccaatgt cgagcaaacc
     1141 ccgcccagcg tcttgtcatt ggcgaattaa ttcgaacacg cagatgcagt cggggcggcg
     1201 cggtcccagg tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc
     1261 ctgcagccaa tatgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg
     1321 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     1381 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     1441 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     1501 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     1561 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     1621 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     1681 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     1741 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     1801 tcaaggcgcg catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc
     1861 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     1921 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     1981 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     2041 tcgccttcta tcgccttctt gacgagttct tctgagggga tcggcaataa aaagacagaa
     2101 taaaacgcac gggtgttggg tcgtttgttc ggatccgtcg acattgatta ttgactagtt
     2161 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     2221 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     2281 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     2341 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     2401 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     2461 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     2521 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
     2581 ttttgtattt atttattttt taattcctag tagcactgat tcggacgcgc cgtgggtcgt
     2641 ttattggtta caggtggggg gccagcgccg ggacacgggg ggggggatgg gggggggggg
     2701 gggggggagg agaagagggg gggggggggg gggggggggg gggggggggg ggcggggcgg
     2761 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
     2821 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
     2881 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
     2941 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
     3001 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
     3061 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg
     3121 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg
     3181 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg
     3241 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     3301 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc
     3361 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc
     3421 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
     3481 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
     3541 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     3601 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
     3661 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     3721 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
     3781 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     3841 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     3901 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaatttgcta gcgagctcgc
     3961 ggccgcagat ctctagttaa gctatcaaca agtttgtaca aaaaagcagg ctctttaaag
     4021 gaaccaattc agtcgacggt acctaccatg tacccgtacg acgtgcccga ctacgcggga
     4081 tccaccatgc aggagagcca gaccaagagc atgttcgtgt cccgggccct ggagaagatc
     4141 ctagccgaca aggaggtgaa gcggccccag cactcccagc tgcgcagggc ctgccaggtg
     4201 gcgctcgatg aaattaaagc agaaatagaa aagcagaggc ttggcactgc tgcaccacca
     4261 aaggcaaact tcattgaagc tgacaagtat tttcttccat tcgagctagc ttgccagtcc
     4321 aagtccccaa gggtagtcag cacatccctt gactgcttgc agaaactcat cgcatacggg
     4381 cacatcactg gcaacgcccc tgacagtgga gcccctggga agcggctgat cgacagaatt
     4441 gttgaaacca tttgcagttg ttttcagggc cctcagactg atgaaggggt tcagttacaa
     4501 ataattaagg ctcttctgac tgcagtgact tccccacaca ttgaaattca tgagggtact
     4561 atcctgcaga cagtgagaac atgttacaat atctatttgg ccagcaaaaa tctcatcaat
     4621 caaaccactg ccaaggctac ccttactcag atgctgaacg tcattttcac ccgcatggaa
     4681 aaccaagtgt tgcaggaggc cagagaactg gaaaaaccaa tccagtcaaa accccagtcc
     4741 cctgtgatcc aagctgcagc agtatcccca aagttcgttc gtttgaagca cagtcaggca
     4801 caaagcaaac caacaactcc cgaaaaaaca gatttaacca acggtgaaca tgccaggagt
     4861 gattctggaa aagtaagcac agaaaatgga gacgcaccca gagaaagagg ctcatcactg
     4921 tcagggactg atgacggagc ccaggaggtg gtgaaggaca tcttggaaga tgtagtcaca
     4981 tctgccatta aagcagcgga aaagcatggt ctgacagaac ctgagagagt tctaggtgaa
     5041 ctggagtgcc aggaatgtgc tattccccca ggagttgatg aaaactcaca gaccaacggg
     5101 atagccgatg acaggcagtc cttgtcgtca gcagataatc tggaatcgga tgcacaagga
     5161 catcaagtgg ctgccaggtt ctcccacgtt ctgcagaagg atgccttcct tgtgttccgc
     5221 tccctgtgca agctgtccat gaaacccctt ggtgaaggcc ctccagaccc aaaatcccat
     5281 gagctgcgtt ccaaggtggt ttccctgcag ctgctcctct ctgtgttgca aaatgctggc
     5341 cccgtattca ggactcacga gatgttcatc aatgcaatca agcaatatct ctgtgtggcc
     5401 ttgtccaaaa acggcgtctc ttcagtgcct gatgtctttg agctctctct tgccattttt
     5461 cttactcttc tttcaaactt taaaatgcac ttgaaaatgc agatagaggt ctttttcaaa
     5521 gagattttcc tgaacatttt agaaacatca acaagttctt ttgagcacag gtggatggtc
     5581 attcagactc tgacgaggat ctgtgcagat gcccagtgtg ttgtggatat ttatgtcaac
     5641 tacgactgtg atttaaatgc tgctaacatt tttgagcgcc ttgtaaatga tttatccaaa
     5701 attgctcagg gaagaagtgg acatgagctg ggaatgacac ctctgcagga gctcagcctg
     5761 aggaagaaag gcctggagtg cctcgtgtcc attctcaagt gcatggtgga gtggagcaaa
     5821 gacctgtatg tgaatcccaa ccaccagacc agcctcggtc aggagaggct cacggatcag
     5881 gaaatagggg atgggaaagg ccttgacatg gcaagacggt gtagtgtgac gtccatggag
     5941 tccacagtgt cctcggggac ccagacaact gttcaggatg accctgagca atttgaggtc
     6001 atcaagcaac aaaaagaaat cattgaacac ggcatcgagc tgttcaacaa gaaacccaag
     6061 agggggatcc agtttctcca ggagcagggc atgctgggaa cgtcagttga agacatagcc
     6121 caattcctgc accaggagga gcgcctggat tccacccaag taggcgattt tctgggagat
     6181 agcgcaaggt tcaacaagga ggtgatgtat gcctacgtgg accaacttga cttctgtgaa
     6241 aaagaatttg tctcagccct gcggacattc ctagaaggtt tccgcctacc tggagaagcc
     6301 caaaagattg accgattaat ggagaagttt gccgcaagat acatagaatg caaccaaggg
     6361 caaactctgt ttgctagtgc tgacactgct tatgtcctag cgtattcaat tattatgctg
     6421 actacagact tgcacagtcc tcaggtaaaa aataaaatga cgaaagagca gtatattaaa
     6481 atgaatcggg gtatcaatga tagtaaagat ctgccagaag agtatctctc aagcatctat
     6541 gaagagatag aaggcaagaa aattgcaatg aaagaaacaa aagagctaag gaaggtgcga
     6601 ataagcgggg aaattcttct cggctgactc agtcatttca tttcttcatg tttgagccga
     6661 ttttttctcc cgtaaatgcc ttgaatcagc ctatttagac cgtttcttcg ccatttaagg
     6721 cgttatcccc agtttttagt gagatctctc ccactgacgt atcatttggt ccgcccgaaa
     6781 caggttggcc agcgtgaata acatcgccag ttggttatcg tttttcagca accccttgta
     6841 tctggctttc acgaagccga actgtcgctt gatgatgcga aatgggtgct ccaccctggc
     6901 ccggatgctg gctttcatgt attcgatgtt gatggccgtt ttgttcttgc gtggatgctg
     6961 tttcaaggtt cttaccttgc cggggcgctc ggcgatcagc cagtccacat ccacctcggc
     7021 cagctcctcg cgctgtggcg ccccttggta gccggcatcg gctgagacaa attgctcctc
     7081 tccatgcagc agattaccca gctgattgag gtcatgctcg ttggccgcgg tggtgaccag
     7141 gctgtgggtc aggccactct tggcatcgac accaatgtgg gccttcatgc caaagtgcca
     7201 ctgattgcct ttcttggtct gatgcatctc cggatcgcgt tgctgctctt tgttcttggt
     7261 cgagctgggt gcctcaatga tggtggcatc gaccaaggtg ccttgagtca tcatgacgcc
     7321 tgcttcggcc agccagcgat tgatggtctt gaacaattgg cgggccagtt gatgctgctc
     7381 cagcaggtgg cggaaattca tgatggtggt gcggtccggc aaggcgctat ccagggataa
     7441 ccgggcaaac agacgcatgg aggcgatttc gtacagagca tcttccatcg cgccatcgct
     7501 caggttgtac caatgctgca tgcagtgaat gcgtagcatg gtttccagcg gataaggtcg
     7561 ccggccatta ccagccttgg ggtaaaacgg ctcgatgact tccaccatgt tttgccatgg
     7621 cagaatctgc tccatgcggg acaagaaaat ctcttttctg gtctgacggc gcttactgct
     7681 gaattcactg tcggcgaagg taagttgatg actcatgatg aaccctgttc tatggctcca
     7741 gatgacaaac atgatctcat atcagggatt tgttcgcacc ttccctaacg attgcaacca
     7801 aatctactaa gcagaatgta gctagtgaaa agcagcggcg gctgctgtac aacttagaga
     7861 tggagcaaat ggctaaaaca gccaaagctc tgatggaggc tgtgagccat gccaaagccc
     7921 cgtttaccag tgccactcac ctggaccatg tccggccaat gttcaaactg gtgtggacgc
     7981 cactattggc agcctacagc atcggactcc agaactgtga tgacactgaa gtggcctcct
     8041 tgtgtttgga aggcatccga tgtgcaatcc gaatcgcctg catctttgga atgcagctgg
     8101 aacgagatgc ctatgttcag gctcttgctc gcttctccct actcacagcc agctccagca
     8161 tcacagaaat gaagcagaaa aacatcgaca ccattaagac gcttatcaca gtggctcaca
     8221 ccgatggcaa ctaccttggg aattcctggc atgagatctt gaaatgcatc agccagctgg
     8281 agctcgctca gctgatagga accggtgtga agacgcgcta cctgtctgga tctgggcgtg
     8341 aaagagaagg gagcctgaag ggccacacat tggcaggaga agagttcatg ggccttggcc
     8401 tcggtaattt ggtgagtggc ggagtggata aaagacagat ggccagcttc caagaatcgg
     8461 ttggtgagac cagctcgcag agtgtggttg tagctgtgga caggattttt actgggtcta
     8521 ccagactgga tggaaatgca atagttgact ttgtccgctg gctgtgtgct gtgtccatgg
     8581 atgaactggc ttccccccac catcctcgca tgttcagctt gcagaagatt gtggagatat
     8641 catactacaa catgaatcgg atccgactac agtggtctcg aatatggcat gtgattggag
     8701 atcacttcaa taaggttggc tgcaacccta atgaagatgt ggctatcttt gctgttgact
     8761 cattaaggca actctccatg aagtttcttg agaagggtga attagccaac ttccgtttcc
     8821 agaaagattt tctgaggccc tttgagcata ttatgaagaa aaacaggtct cccaccatcc
     8881 gggacatggc gatccgctgc attgcccaga tggtgaactc ccaggcggcc aacatccgct
     8941 caggctggaa gaacatcttt gccgtgttcc accaggcagc ctctgatcat gatgggaaca
     9001 ttgtggagct ggccttccag accacttgcc acattgtcac aactattttc cagcaccatt
     9061 ttcctgcagc catcgattcc tttcaggatg ctgtgaagtg cttatcagag ttcgcctgca
     9121 acgccgcttt ccctgacacg agcatggaag cgattcggct catccgcttc tgtggcaaat
     9181 acgtctctga gaggcctcgg gtgctacaag aatacacaag tgatgacatg aatgtagctc
     9241 ctggtgacag agtctgggtc cgaggctggt tccccatctt attcgaactc tcctgcatca
     9301 ttaatagatg caagttagat gtacgaacaa ggggactcac agtcatgttt gagatcatga
     9361 agagctatgg ccacaccttt gaaaagcact ggtggcagga cctgttcaga atcgtgtttc
     9421 ggatttttga caatatgaaa ctccctgagc aactgtcaga gaaatctgag tggatgacaa
     9481 caacctgcaa tcacgcactt tatgctattt gtgatgtttt tacccagttt tatgaagctt
     9541 tgaatgaagt tcttctttct gatgtatttg cacaattgca gtggtgtgtc aaacaagata
     9601 atgaacagtt ggcgcgatca ggtacaaatt gcttagaaaa cttagtaata tccaatggag
     9661 agaaattcag tcctgaagtc tgggatgaaa cctgcaactg tatgttggat attttcaaaa
     9721 caaccatccc acatgttttg ctgacatgga gacctgtagg aatggaggaa gattcatcag
     9781 aaaagcattt ggatgtggat ctggaccgcc agtctttaag cagcatagat aaaaatccct
     9841 ctgagagggg acagagccag ctctctaacc caacagatga cagctggaag ggtagaccat
     9901 acgcaaatca gaaactgttt gccagcctcc tcatcaagtg tgtggtccag ttggaattga
     9961 tacagaccat tgacaacatt gtgttctacc ctgcgacgag caaaaaggag gatgcagagc
    10021 acatggttgc cgcccagcaa gacacgctgg atgcagatat ccacatagag acggaggatc
    10081 agggcatgta taagtacatg tcttcccagc acctcttcaa gctgttggac tgtttgcagg
    10141 aatcccattc attctcaaag gccttcaact ccaattacga gcagcggact gtcctgtggc
    10201 gagcaggttt taagggcaag tctaaaccca atcttctaaa acaagaaacc agcagcctgg
    10261 cctgttgttt gaggatcctg tttcgaatgt atgttgatga gaaccgcagg gattcctggg
    10321 aagaaataca gcagagactt ttaactgttt gcagtgaagc tcttgcctat ttcatcactg
    10381 tgaattctga gagccatcgg gaggcctgga caagtctctt gttgttactt ctaactaaaa
    10441 cccttaagat aaatgatgaa aagttcaaag cacatgcttc aatgtactac ccctacttgt
    10501 gtgaaattat gcagtttgac ctgatccctg agctccgagc agttctgcgg aagttcttcc
    10561 tacggatagg tgttgtgtat aagatatgga taccagaaga gccatcacag gtaccagcag
    10621 cactgtcacc agtgtggtag ccctggctgc ccaggccagt gctgcagctc tgcagaatgt
    10681 tcagcatgcc atttctgact ggcacatctc gtgaagtttc atagaaacaa ggagttggca
    10741 tcttggatct cagaatggcc tggaaacgga tggcctctac gctgttccat cacagtctcc
    10801 aactaaggct tatggtattt cattaaactg ttgcataccc agttagcaca gtaggtgggg
    10861 agtctgcttc atttctatca ttccattttt ctgattaaac tgtcaaatct gtcattgcat
    10921 atgccatcgt tttctagcaa aatcccatga ttggctataa acgttttgta agaagtcact
    10981 ctccttgaaa atactgaaca tagctgtata ggtttgtgat tattagagaa tatgttaata
    11041 aaacttcttg taacggctaa ctgccaccta aaataaaaaa aaaaaaaaaa aacgtttgcg
    11101 gccgcactcg agatatctag acccagcttt cttgtacaaa gtggttgatc tagagggccc
    11161 gcggttcgaa ctcgaggtac cgaattcgta cccatacgac gtcccagact acgcttaatt
    11221 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
    11281 tcacaaatac cactgagatc gatctttttc cctctgccaa aaattatggg gacatcatga
    11341 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
    11401 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
    11461 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
    11521 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
    11581 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
    11641 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
    11701 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
    11761 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    11821 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    11881 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    11941 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
    12001 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    12061 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    12121 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
    12181 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    12241 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
    12301 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    12361 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    12421 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    12481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    12541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    12601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    12661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    12721 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    12781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    12841 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    12901 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    12961 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    13021 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    13081 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    13141 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    13201 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    13261 agtatatatg agtaaacttg gtctgacagt tacca
//
