LOCUS       sequence-454352-        8228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TF A self-activation cassette in MultiFate-2.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Zhu R, Del Rio-Salgado JM, Garcia-Ojalvo J, Elowitz MB
  TITLE     Synthetic multistability in mammalian cells.
  JOURNAL   Science. 2022 Jan 21;375(6578):eabg9765. doi: 
            10.1126/science.abg9765. Epub 2022 Jan 21.
  PUBMED    35050677
REFERENCE   2  (bases 1 to 8228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-01-21"; volume: "375(6578):eabg9765. doi"; pages: " 
            10.1126/science.abg9765. Epub 2022 Jan 21"
COMMENT     Sequence Label: 
            MF81_PB-TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR3
            9A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
FEATURES             Location/Qualifiers
     source          1..8228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1390..1408
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1426..1444
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1462..1480
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1498..1516
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1534..1552
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1570..1588
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1945..1969
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2053..2062
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2065..2085
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2089..2409
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             2932..2952
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2962..2985
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(3481..4055,4056..4058,4059..4067)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3648..3665)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3875..3894
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4068..4775
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4213..4231)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4481..4500
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4936..4953)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4942..5149
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5682..5716
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6207..6223)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6207..6223)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6220..6242)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6231..6247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6255..6261,6262..6279,6280..6285))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6300..6321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6438..6455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6609..7197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6689..6708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7368..8159,8160..8228))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7991..8010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtggcctcca cggccacaag
     1381 cttcatatgt ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     1441 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     1501 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     1561 acagtttact ccctatcagt gatagagaac gtatatccag tttactcccg acgctgctga
     1621 cgctgctgac gctgctgacg ctgctgacgc tgctgacgct gctgacgctg ctgacgctgc
     1681 tgacgctgct gacgctgctg acgctgctga cgctgctcaa ttgtcgccgg cggcagaagc
     1741 cgcagaagcc tctagagcgc agaagccgca gaagccatcg atatgcagaa gccgcagaag
     1801 cctcacgtac gcagaagccg cagaagccta tgacctgcag aagccgcaga agccacgtat
     1861 atgcagaagc cgcagaagcc cctagaccgg ttgatagaga acgtataagc tttaggcgtg
     1921 tacggtgggc gcctataaaa gcagagctcg tttagtgaac cgtcagatcg cctggagcaa
     1981 ttccacaaca cttttgtctt ataccaactt tccgtaccac ttcctaccct cgtaaatcga
     2041 cgaagcttct aggccaccat gggacccaag aaaaagcgga aggtgtccgg agtgcaggtg
     2101 gagactatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg
     2161 cactacaccg ggatgcttga agatggaaag aaagttgatt cctcccggga cagaaacaag
     2221 ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc
     2281 cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc
     2341 actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt ggagcttcta
     2401 aaactggaag gatccgagcg gccattccag tgcagaatct gcatgcggaa cttctccgac
     2461 tctcccacac tgcggagaca caccagaaca cacacaggcg agaagccctt ccagtgccgg
     2521 atctgtatgg ccaacttttc ccagggcgcc aacctgagaa ggcacctgag aacccatacc
     2581 ggcgaaaagc cttttcagtg tcgcatctgc atgagaaact ttagccaggc taacaccctg
     2641 cagcgccatc tgaaaaccca cactggcgag aaaggaggcg gatcctctgg cgctcctcct
     2701 accgatgtgt ctctgggaga tgagctgcac ctggacggcg aggatgtggc tatggctcat
     2761 gccgacgctc tggacgactt cgacttggat atgctcggcg acggcgactc tcctggacct
     2821 ggctttaccc ctcatgactc tgccccttac ggcgccctgg atatggccga tttcgagttc
     2881 gagcagatgt tcaccgatgc tctgggcatc gatgagtacg gcggaggctc ccccaagaaa
     2941 aagcggaagg tgggctcgag cgactacaag gacgacgacg acaagtcgag catctccctg
     3001 atcgctgccc tggctgtgga ttacgtgatc ggaatggaaa acgccatgcc atggaacctg
     3061 cctgctgacc tggcctggtt caagcggaac accctgaaca agcccgtgat catgggcaga
     3121 cacacctggg agtctatcgg cagacctctg cctggccgga agaacatcat cctgtcctct
     3181 cagccttcca ccgacgacag agtgacctgg gtcaagtctg tggacgaggc tatcgctgct
     3241 tgtggcgacg tgccagagat catggttatc ggcggaggca gagtgatcga gcagttcctg
     3301 cctaaggctc agaagctgta tctgacccac atcgacgccg aagtggaagg cgatacccac
     3361 tttcctgact acgagcccga cgactgggag tccgtgttct ctgagttcca cgacgccgat
     3421 gctcagaact cccactccta ctgcttcgag atcctggagc ggagataggt cgaccagatc
     3481 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     3541 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     3601 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     3661 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     3721 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     3781 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     3841 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     3901 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     3961 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     4021 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg gtgagcaagg
     4081 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     4141 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     4201 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     4261 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     4321 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     4381 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     4441 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     4501 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     4561 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     4621 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     4681 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     4741 gccactccac cggcggcatg gatgagctgt acaagtccgg caagcttagc catggcttcc
     4801 cgccggaggt ggaggagcag gatgatggca cgctgcccat gtcttgtgcc caggagagcg
     4861 ggatggaccg tcaccctgca gcctgtgctt ctgctaggat caatgtgtga gctagcgaat
     4921 tcgaatttaa atcggcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4981 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     5041 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     5101 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggac gcggccgcgg
     5161 gcccattggt atggcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc
     5221 gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc
     5281 acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc
     5341 gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt gggggggggc
     5401 tgtccctgat atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca
     5461 tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca
     5521 tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga
     5581 caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg
     5641 attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca
     5701 gactatcttt ctagggttaa tctagctgca tcaggatcat atcgtcgggt cttttttccg
     5761 gctcagtcat cgcccaagct ggcgctatct gggcatcggg gaggaagaag cccgtgcctt
     5821 ttcccgcgag gttgaagcgg catggaaaga gtttgccgag gatgactgct gctgcattga
     5881 cgttgagcga aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt
     5941 taacggtgaa ctgttcgttc aggccacctg ggataccagt tcgtcgcggc ttttccggac
     6001 acagttccgg atggtcagcc cgaagcgcat cagcaacccg aacaataccg gcgacagccg
     6061 gaactgccgt gccggtgtgc agattaatga cagcggtgcg gcgctgggat attacgtcag
     6121 cgaggacggg tatcctggct ggatgccgca gaaatggaca tggatacccc gtgagttacc
     6181 cggcgggcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6241 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6301 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6361 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6421 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6481 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6541 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6601 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6661 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6721 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6781 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6841 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6901 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6961 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7021 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7081 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7141 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7201 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7261 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7321 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7381 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7441 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7501 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7561 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7621 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7681 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7741 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7801 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7861 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7921 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7981 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8041 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8101 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8161 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8221 atactcat
//
