LOCUS       sequence-454354-       10496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGIGYF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Ruscica V, Bawankar P, Peter D, Helms S, Igreja C, Izaurralde E
  TITLE     Direct role for the Drosophila GIGYF protein in 4EHP-mediated mRNA 
            repression.
  JOURNAL   Nucleic Acids Res. 2019 Jul 26;47(13):7035-7048. doi: 
            10.1093/nar/gkz429.
  PUBMED    31114929
REFERENCE   2  (bases 1 to 10496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-07-26- 26"; volume: "47"; issue: 
            "13"; pages: "7035-7048"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmGIGYF_AH
FEATURES             Location/Qualifiers
     source          1..10496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2310..2312,2313..2315,2316..3026)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2310..2312,2313..2315,2316..3026)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2355..2376)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2616..2635)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2963..2984
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7802..7843
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7853..7870
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7893..7910)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7943..8077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7967..7986)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8021..8040
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8678..9266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8758..8777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9437..10228,10229..10297))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10060..10079
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10298..10402)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10470..10488
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat gcggaggaga aggagaacga gctgatctag tatctctcca caatccaatg
       61 ccaactgacc aactggccat attcggagca atttgaagcc aatttccatc gcctggcgat
      121 cgctccattc ttggctatat gtttttcacc gttacccggg gccattttca aagactcgtc
      181 ggcaagataa gattgtgtca ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat
      241 ttcgcgatga cgtcggcgag tattttgaag aatgagaata atttgtattt atacgaaaat
      301 cagttagtgg aattttctac aaaaacatgt tatctataga taattttgtt gcaaaatatg
      361 ttgactatga caaagattgt atgtatatac ctttaatgta ttctcatttt cttatgtatt
      421 tataatggca atgatgatac tgatgatatt ttaagatgat gccagaccaa aaggcttgaa
      481 tttctgcgtc ttttgccgaa cgcagtgcat gtgcaattgt tgttttttgg aatattcaat
      541 tttcggactg tccgctttga tttcagtttc ttggcttatt caaaaagcaa agtaaagcca
      601 aaaaagcgag atggcaatac caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg
      661 gcagcggaag gaagggtggg gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc
      721 gacgttggag ccactccttt gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact
      781 cgccggttgt ttttttcttt ttatgctgcg ctctctctag cgccatctcg cttacgcatg
      841 ctcaacgcac cgcatgttgc cgtttccttt tatgcgtcat tttggctcga aataggcaat
      901 tatttaaaca aagattagtc aacgaaaacg ctaaaataaa taagtctaca atatggttac
      961 ttattgccat gtgtgtgcag ccaacgatag caacaaaagc aacaacacag gtggctttcc
     1021 ctctttcact ttttgtttgc aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa
     1081 ttacgctgac aaagagcaga cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat
     1141 gcatttgcaa taacaattgc gatatttaat attgtttatg aagctgtttg acttcaaaac
     1201 acacaaaaaa aaaaataaaa caaattattt gaaagagaat taggaatcgg acgcttatcg
     1261 ttagggtaac aacaagaaat gcttactgag tcacagcctc tggaaaactg ccgcaagcca
     1321 gagagagaga gaaaaagagg gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc
     1381 tctctcttcg tctctgttgc gcaaacgcat agactgcact gaaaaaatcg attacctatt
     1441 ttttatgaat gaatatttgc actattacta ttcaaaacta ttaagatagc aatcacattc
     1501 aatagccaaa tactatacca cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg
     1561 ctgcatattt aggacggcat cattatagaa atgcttcttg ctgtgtactt ttctctcgtc
     1621 tggcagctgt ttcgccgtta ttgttaaaac cggcttaagt taggtgtgtt ttctacgact
     1681 agtgaatgcc ctactagaag atgtgtgttg cacaaaatgt ccctggaata accaatttga
     1741 agtgcagata gcagtaaacg taagctaata tgaatattat ttaactgtaa tgttttaata
     1801 tcgctggaca ttactaataa acccactata aacacatgta catatgtatg ttttggcata
     1861 caatgagtag ttggggaaaa aatgtgtaaa agcaccgtga ccatcacagc ataaagataa
     1921 ccagctgaag tatcgaatat gagtaacccc caaattgaat cacatgccgc aactgatagg
     1981 acccatggaa gtacactctt catggcgata tacaagacac acacaagcac gaacacccag
     2041 ttgcggagga aattctccgt aaatgaaaac ccaatcggcg aacaattcat acccatatat
     2101 ggtaaaagtt ttgaacgcga cttgagagcg gagagcattg cggctgataa ggttttagcg
     2161 ctaagcgggc tttataaaac gggctgcggg accagttttc atatcactac cgtttgagtt
     2221 cttgtgctgt gtggatactc ctcccgacac aaagccgctc catcagccag cagtcgtcta
     2281 atccagagac cccggatcgg ggtaccatca tggtgagcaa gggcgaggag ctgttcaccg
     2341 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     2401 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     2461 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     2521 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     2581 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     2641 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     2701 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     2761 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     2821 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     2881 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     2941 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3001 ctctcggcat ggacgagctg tacaagccaa gcttgaattc tatgacagat tcaatgaaat
     3061 ttggcccgga atggttgcgc aatatgtcag ccgagccttc gggctctccc agtacctaca
     3121 acgttggtaa tgcagctcaa aacaattcga ttggagggca caacctggga aacaacacgg
     3181 cggcatccgc ttcccgtaat ctatttccag aataccggta cggacgcgag gaaatgctgt
     3241 ccttgttcga tcggaactgc ctactgccgc agatcctgcc atcgttcaaa aagctcttcg
     3301 tggaaaaggt tcagtgccct cttgcactga caccgagctc cgaggaggac atcaaccaaa
     3361 actcacttgg caataactct cgtcctgcct ggttgcagcg ctcgcccagt ggatttggta
     3421 gtgcctcccg aggctctgga cgtggtggaa ccgttgaccg gggacggatg cgcggaaaat
     3481 cggcctatca tccgatatac caacgcccaa gcggtctata tgatgaaagc ttatcggtta
     3541 tatcaaaggc cgaacgcaca tggagcgacc gcaacggaac tggagattct gcggcaacca
     3601 ccacctccac tagtggctct ggtgctctag attggaatgg aactccaagc tcaagtcctc
     3661 gaaaagatta ttctaataat catcgcaact tggaaaactg gcgacgaaca cgtaacgaag
     3721 atggatccgg tgatggtcca tcctccagcg gttccatgag cggacccgat attgctggtt
     3781 ggcggagcgg tgtcgtcggt ggaagtacaa acgcaggttt tggtaccaat agccatcggt
     3841 ggggaagatc tacaagctgg cgtgatgaag atcctagcgt cgacaatgca gctagtttac
     3901 aacgctcaat ttcaacagtt gcgactttaa gtacagatcg gactggaaac aataaaggat
     3961 ctggaatcgg agctgcagag ggagtaggga gcatatcgca cccacggttg tcgagttcta
     4021 aaatatctca attatggact gttaataatg cagccggtgt tgatgccgac gaaaatctac
     4081 ccgaatgggc aattgaaaat ccatcgaaat tgggtggcag ttttgatgct agtggagcct
     4141 ttcatggaga tactgatcta aaacctatca aaagttcgca taatacttta aaaaccaaaa
     4201 gcttagactc ctatgacgat gtaaaaagac cgaaaagtaa agacctgtct gatccagata
     4261 gtggaaacga tataactcca gaaacctcat taacgaaaga taccaataca acagctgtgc
     4321 aagaagaagt tgaaagcagt ttatctccaa taagctctac aacgacaaag gaagtgatcc
     4381 atggagatat ttcagatcgg attaaagagg tcgccgatga agtagaaaaa cttataatgg
     4441 atgatgatca taaaatctct tcgaatcaaa gccaacatca aaatcatgac gggtttacag
     4501 ctgcacttcc aagaatggca gacattgaga taaacgtaaa accgagtgtt acagcagtgc
     4561 atcggcaagc accttccacc atgcctatac aaattacccc tacaattaca gatgtcgctc
     4621 caccatccca tgcagttgtg tcgttttcgg atcatgagac aatgcaacac cataacatgc
     4681 accacctgcc acaatttcca atgattccta caccgcatat catcactcca aatctaaacg
     4741 aattgtggtt ttaccgggat ccgcaggcaa atgttcaggg gccatttagt gccgttgaga
     4801 tgacggaatg gtatcgcgct ggctacttta atgagaacct gtttgtacgc cggtactctg
     4861 ataataggtt tagaccgctg ggagagctta taaaattttg tcatggtaac atgccattta
     4921 cgcacagtca cctacttcca tctccgatag aactagagaa cattcctgtt ggtcaaatac
     4981 cagcccctct aacggcgtca ctttcaatta caccccataa gccatcaccg attcctatcg
     5041 cactttctgt tgttgaacag cagttgcagc aacaaagaga tgagcaacta aaggcaaatg
     5101 ttacagcaac agctgaagct ctacgtgctg caataaaagg aagttttggc ggaaatagca
     5161 ttggtaatac gtctcatttg cttacaatgc ggtttcaaat gctacaggat cagtacatac
     5221 agcaccagga ataccaaata ctagctgagc tatccaaaaa tgaatgcttt caacgtcttt
     5281 cggctgtcga gcaagaaaca gttgttcgtc ggaaagttca attgctgggt cttcctgagt
     5341 atttgatcag cttaaacgga ttaagcaatt ctttgtccgt actgaacccc gttgccggta
     5401 gacagttgta cagggcagtg gttgagcacg ccaagaagga tcaacaacat atttttgcaa
     5461 acaccgagca acaacgttca gtgggaaatt tactggatgc taataatttc attctaaatg
     5521 cccaaataat gattcagcaa tcgcagcaag aggtaggtcc cttggtatca tccgtggatt
     5581 gtattatgca aggtggaact gcagctgacc taaataagcc gaatgaaata ccgaggaatg
     5641 agttagactt aattaatgaa tataacttac ggatgctgtt gcgtggccaa ccaacaagta
     5701 ctcaacagca accacctgcg ctgacgaact cagctaccga aaatctccct ggagttgatt
     5761 ttttaactga aacacaattg ttagagcggc aaaacttgat gattccaatc tggttacccc
     5821 ctaataataa taagcaacaa gaaaccgacc aacagtgggc tgaaatgtct aatgcggatg
     5881 catcattatg ggaaacggcc aacttaaatg aagagcgaaa tgaagatcag caactattaa
     5941 tacaaaagtc ttctgaagcg tgcttcgcag atacagaaaa agatgtcaaa attgcccaat
     6001 tatttcaagt tcaatcagga aatgttgtta accacaccgc attagaggaa ttagatcagt
     6061 cccctcaaaa tttaaaaggt tcacataatc aaaaaatagt caaatccctt gtttccgata
     6121 ttcaacagaa tcataatgag gaactaaatt cacatcaaca ccaggtaaag caggctaaca
     6181 agcagaatct gaatacaaaa cagaatgcgg cacagtcagc cctaaagcca attaataacg
     6241 aaaatgatcg caaacgagaa cagacagaag aaaaaaaaag gcaaagagag gaacgcaagc
     6301 gccagcaatt ggaagatgat aaacgtcggg cgttgaatga atctgaagaa cagacccgcc
     6361 aaattcaaga ggaaaaggaa aggcaacagc aaatacaagc ccaacgtcga aaggcattgt
     6421 tgggcaatgt ccattctcta tcagttcaaa atgggatgtc aggaacatta gcgtctgcac
     6481 aaagcaagaa gaacgacgat gccaaaacag cagaacccca agtatcctcg cgcttgccgt
     6541 ctacatccgt agcgccttgg tcttttcagt tgcaaaattc tatgagatcg gcgcctggtc
     6601 tggcagaaat acaaaaggca gaacgtcgag agcgtcgcgc agaccaacag cgacatcaag
     6661 agctattaga caagcaattg cgtgccaatg ccgcagctgc ggctgaagcc aacgatgctt
     6721 tgcttaaatg gcagtcaaca ccagcgtcgg cgcccgtaat gagtctcgcc gaaattcaag
     6781 cagaagaggc aaggcggttg gccaatgacc ttgtggatcg gcagcgtcga cgagaactgg
     6841 aacatcacca acaagctcct ttgtcatcag cggttttggt tacgagtgca acttccaaca
     6901 tctggggtaa cgccaataaa gcatggagtt cgtccgctgc tcaatcactt tcgttaaaaa
     6961 caagttctgg aactggtctg tgggacgaac cgaatccatt aggttctaat ggatctggaa
     7021 caagcggtac ctcatccgta actgcggcgg cagtcttggc aggaggatta aactcagcta
     7081 ataaatccac tctgcaagct caaaataagt cgtcggcttt atttgcatcg cctcgaaatt
     7141 tgcgcaagag tcaaacattg ccagccctta gtaacccaga aaagtcaaat aaaaatggac
     7201 caggtcaacg gccagaaaaa caaaagttgg ctcaaactcg ttcaaagggt gccgctgtct
     7261 caattgaaga gaaggataga gaaagaaaat tgaatgcaaa aagccagcaa agcagcaccg
     7321 accaagccat tagcaaggtt aatgagtatg aaaacgagtt cactagctgg tgcataaaga
     7381 gcctagataa tatgtccgcc aaagttgatg tacccacgtt cgttgcattc ttgcaggact
     7441 tggaagcgcc atatgaggta aaggactatg tccgaatata ccttggcgat ggaaaagatt
     7501 ctttggattt cgcaaagcag tttttggagc gacgtagcaa atacaaaagc ttgcaacgtg
     7561 cccaaaaggc acacaatgac gatatgtgca aaccagctcc tgccattacg ccatctgcga
     7621 atgactatgc cgacagcaag aacaaacaaa aaaagattaa aaagaataag atgactaaga
     7681 tggacgcccg cattcttggg ttttcagtaa ctgctgccga gggtcgcata aatgttggca
     7741 ttcgagacta cgtcgaagga ccataagcgg ccgctcgagt ctagagggcc cgcggttcga
     7801 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca
     7861 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta aggcctgagc
     7921 tcgctgatca gcctcgatcg aggatccaga catgataaga tacattgatg agtttggaca
     7981 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     8041 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     8101 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     8161 atgtggtatg gctgattatg atcagtcgac cgatgccctt gagagccttc aacccagtca
     8221 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     8281 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     8341 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     8401 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     8461 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     8521 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     8581 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggccagcaaa
     8641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9061 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     9121 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9181 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9241 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9301 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9361 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9421 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9481 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9541 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9601 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9661 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9721 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     9781 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9841 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9901 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9961 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10021 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
    10081 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10201 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10261 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10321 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10381 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    10441 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgt
//
