LOCUS       sequence-454361-       11589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For loading SpCas9 into PVCs; also contains PVC regulatory genes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11589)
  AUTHORS   Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F
  TITLE     Programmable protein delivery with a bacterial contractile injection
            system.
  JOURNAL   Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7.
            Epub 2023 Mar 29.
  PUBMED    36991127
REFERENCE   2  (bases 1 to 11589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-05870-7"; journalName: "Nature"; date: 
            "2023-04"; volume: "616"; issue: "7956"; pages: "357-364"
COMMENT     Sequence Label: pBR322-Pdp1_NTD-Cas9
FEATURES             Location/Qualifiers
     source          1..11589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     173..191
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(218..223,224..240,241..246)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1148..1168
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1244..1264
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1289..5392
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1289..5392
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9351..9542
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(9536..9558)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9644..9784
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9699..9718
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9799..9816)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9970..10558)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10050..10069)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10729..11520,11521..11589))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11352..11371
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
      181 tatcacgagg ccctttcgtc ttcaagaatt ctcatgtttg acagcttatc atcgataagc
      241 tttaatgcgg tagtttatca cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat
      301 ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct
      361 tggttatgcc ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca
      421 gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc
      481 tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag
      541 ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatccat ttttttaatt
      601 aaataaagga attaatattg atttgataga gcatgtaatc aataacttct atgaaaatgt
      661 tgaaaaacaa tacacggaaa cacttaactt acaattaaat tgttaaagga gatgatgata
      721 agaatggtca gtcaccagat aaaatgatct ctggtcaatt ttaggaaagc tgaaaaaacc
      781 tgaccaaatg cgtcgtaaat tctatgctta gaaagactcg gtgccagtgt aatacctaag
      841 atatggtttt tatttccata tctgttcgaa aacttagaaa taacaagttc aacaataacc
      901 agggacttta aatatgccta gatatgctaa ttatcagata aaccccaaac agaatattaa
      961 aaattcacat gggaaatctt catcgtcaga tttttctagt gggtaccttt cattctcaaa
     1021 taattcgctt gatgaccctt ttattcggca gcaagttaag agagaattta tttgggaggg
     1081 acatatgaag gagattgaag aagcttcaag attaggtggc tctggtggtg gcggttctgg
     1141 cggcgcaccg aaaaagaaac gcaaagtttg ggagggacat atgaaggaga ttgaagaagc
     1201 ttcaagatta ggtggctctg gtggtggcgg ttctggcggc gcaccgaaaa agaaacgcaa
     1261 agttggtatt catggtgttc cggcagcaat ggataagaaa tactcaatag gcttagatat
     1321 cggcacaaat agcgtcggat gggcggtgat cactgatgaa tataaggttc cgtctaaaaa
     1381 gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa aaaaatctta taggggctct
     1441 tttatttgac agtggagaga cagcggaagc gactcgtctc aaacggacag ctcgtagaag
     1501 gtatacacgt cggaagaatc gtatttgtta tctacaggag attttttcaa atgagatggc
     1561 gaaagtagat gatagtttct ttcatcgact tgaagagtct tttttggtgg aagaagacaa
     1621 gaagcatgaa cgtcatccta tttttggaaa tatagtagat gaagttgctt atcatgagaa
     1681 atatccaact atctatcatc tgcgaaaaaa attggtagat tctactgata aagcggattt
     1741 gcgcttaatc tatttggcct tagcgcatat gattaagttt cgtggtcatt ttttgattga
     1801 gggagattta aatcctgata atagtgatgt ggacaaacta tttatccagt tggtacaaac
     1861 ctacaatcaa ttatttgaag aaaaccctat taacgcaagt ggagtagatg ctaaagcgat
     1921 tctttctgca cgattgagta aatcaagacg attagaaaat ctcattgctc agctccccgg
     1981 tgagaagaaa aatggcttat ttgggaatct cattgctttg tcattgggtt tgacccctaa
     2041 ttttaaatca aattttgatt tggcagaaga tgctaaatta cagctttcaa aagatactta
     2101 cgatgatgat ttagataatt tattggcgca aattggagat caatatgctg atttgttttt
     2161 ggcagctaag aatttatcag atgctatttt actttcagat atcctaagag taaatactga
     2221 aataactaag gctcccctat cagcttcaat gattaaacgc tacgatgaac atcatcaaga
     2281 cttgactctt ttaaaagctt tagttcgaca acaacttcca gaaaagtata aagaaatctt
     2341 ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat gggggagcta gccaagaaga
     2401 attttataaa tttatcaaac caattttaga aaaaatggat ggtactgagg aattattggt
     2461 gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc tttgacaacg gctctattcc
     2521 ccatcaaatt cacttgggtg agctgcatgc tattttgaga agacaagaag acttttatcc
     2581 atttttaaaa gacaatcgtg agaagattga aaaaatcttg acttttcgaa ttccttatta
     2641 tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg atgactcgga agtctgaaga
     2701 aacaattacc ccatggaatt ttgaagaagt tgtcgataaa ggtgcttcag ctcaatcatt
     2761 tattgaacgc atgacaaact ttgataaaaa tcttccaaat gaaaaagtac taccaaaaca
     2821 tagtttgctt tatgagtatt ttacggttta taacgaattg acaaaggtca aatatgttac
     2881 tgaaggaatg cgaaaaccag catttctttc aggtgaacag aagaaagcca ttgttgattt
     2941 actcttcaaa acaaatcgaa aagtaaccgt taagcaatta aaagaagatt atttcaaaaa
     3001 aatagaatgt tttgatagtg ttgaaatttc aggagttgaa gatagattta atgcttcatt
     3061 aggtacctac catgatttgc taaaaattat taaagataaa gattttttgg ataatgaaga
     3121 aaatgaagat atcttagagg atattgtttt aacattgacc ttatttgaag atagggagat
     3181 gattgaggaa agacttaaaa catatgctca cctctttgat gataaggtga tgaaacagct
     3241 taaacgtcgc cgttatactg gttggggacg tttgtctcga aaattgatta atggtattag
     3301 ggataagcaa tctggcaaaa caatattaga ttttttgaaa tcagatggtt ttgccaatcg
     3361 caattttatg cagctgatcc atgatgatag tttgacattt aaagaagaca ttcaaaaagc
     3421 acaagtgtct ggacaaggcg atagtttaca tgaacatatt gcaaatttag ctggtagccc
     3481 tgctattaaa aaaggtattt tacagactgt aaaagttgtt gatgaattgg tcaaagtaat
     3541 ggggcggcat aagccagaaa atatcgttat tgaaatggca cgtgaaaatc agacaactca
     3601 aaagggccag aaaaattcgc gagagcgtat gaaacgaatc gaagaaggta tcaaagaatt
     3661 aggaagtcag attcttaaag agcatcctgt tgaaaatact caattgcaaa atgaaaagct
     3721 ctatctctat tatctccaaa atggaagaga catgtatgtg gaccaagaat tagatattaa
     3781 tcgtttaagt gattatgatg tcgatcacat tgttccacaa agtttcctta aagacgattc
     3841 aatagacaat aaggtcttaa cgcgttctga taaaaatcgt ggtaaatcgg ataacgttcc
     3901 aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga caacttctaa acgccaagtt
     3961 aatcactcaa cgtaagtttg ataatttaac gaaagctgaa cgtggaggtt tgagtgaact
     4021 tgataaagct ggttttatca aacgccaatt ggttgaaact cgccaaatca ctaagcatgt
     4081 ggcacaaatt ttggatagtc gcatgaatac taaatacgat gaaaatgata aacttattcg
     4141 agaggttaaa gtgattacct taaaatctaa attagtttct gacttccgaa aagatttcca
     4201 attctataaa gtacgtgaga ttaacaatta ccatcatgcc catgatgcgt atctaaatgc
     4261 cgtcgttgga actgctttga ttaagaaata tccaaaactt gaatcggagt ttgtctatgg
     4321 tgattataaa gtttatgatg ttcgtaaaat gattgctaag tctgagcaag aaataggcaa
     4381 agcaaccgca aaatatttct tttactctaa tatcatgaac ttcttcaaaa cagaaattac
     4441 acttgcaaat ggagagattc gcaaacgccc tctaatcgaa actaatgggg aaactggaga
     4501 aattgtctgg gataaagggc gagattttgc cacagtgcgc aaagtattgt ccatgcccca
     4561 agtcaatatt gtcaagaaaa cagaagtaca gacaggcgga ttctccaagg agtcaatttt
     4621 accaaaaaga aattcggaca agcttattgc tcgtaaaaaa gactgggatc caaaaaaata
     4681 tggtggtttt gatagtccaa cggtagctta ttcagtccta gtggttgcta aggtggaaaa
     4741 agggaaatcg aagaagttaa aatccgttaa agagttacta gggatcacaa ttatggaaag
     4801 aagttccttt gaaaaaaatc cgattgactt tttagaagct aaaggatata aggaagttaa
     4861 aaaagactta atcattaaac tacctaaata tagtcttttt gagttagaaa acggtcgtaa
     4921 acggatgctg gctagtgccg gagaattaca aaaaggaaat gagctggctc tgccaagcaa
     4981 atatgtgaat tttttatatt tagctagtca ttatgaaaag ttgaagggta gtccagaaga
     5041 taacgaacaa aaacaattgt ttgtggagca gcataagcat tatttagatg agattattga
     5101 gcaaatcagt gaattttcta agcgtgttat tttagcagat gccaatttag ataaagttct
     5161 tagtgcatat aacaaacata gagacaaacc aatacgtgaa caagcagaaa atattattca
     5221 tttatttacg ttgacgaatc ttggagctcc cgctgctttt aaatattttg atacaacaat
     5281 tgatcgtaaa cgatatacgt ctacaaaaga agttttagat gccactctta tccatcaatc
     5341 catcactggt ctttatgaaa cacgcattga tttgagtcag ctaggaggtg acaaacgtcc
     5401 ggcagcaacc aaaaaggcag gtcaggccaa aaagaaatga tatggatttt catgtcatca
     5461 ggagaatatt caaaattctg tgtcacacaa aatattttac agcgtaatca caccattaag
     5521 aggggcatca aaataaatgg ttaactggga caatgttgaa tttcattttt ataaggcatt
     5581 gtccgtatta tccaccatcc agatcaacgg attcagatgg tgtaaagaaa gcatatagct
     5641 ggtaggtaaa attaagaagt gtagttttat aaagtattaa tggagaacga tgaatttaaa
     5701 gtaaatggag gaaggttagt ttcactatca aattacagga ccaaaaatat ggagtattcc
     5761 atcttctttg gtgaattaag tcttgaagac ttcaacaaat attgcctgga actatgggcc
     5821 tgtgaatggt attggagtat catttataac gactatttcg ataggaaatt taaagtcttg
     5881 ttgaaaatga ctaatcgtct aaaagtatgt ctggagaaat aggtttaatc tctatattta
     5941 acggttttat tttcatctat ttactaatgg taaatttttt tactctttga atatattggt
     6001 gagtttgtta tccccattag ttaccagggt attattaatg agtgaaattc aattttattg
     6061 atattaaagt caaatttaca aatgatgact ttattatttt ttatttatta attttaaatt
     6121 attttttgtt aatctttact ataaatgaat tttttgcttg catggacttg atttttttat
     6181 atataacatt gacaaatatt aattcatata tatgattgtt atagtgagtt atttaattga
     6241 ttcgactaac aatttcattg taatttttca agatagcagt caattgctat aacaagcatc
     6301 tttctcgtca aaggaaaatg tgtatggcct ataagcagca acttgaaaga tgaaggatat
     6361 aatgatgatt aacttaacgg agagtagata ttagaagtag tagaaggaca atttaagact
     6421 ttatacccat taaaacaaag gggtaaaata atgatgtttg atgaaaattt agaatgtgtt
     6481 aatcacgaaa ttatcaatga aaaattagat acgaaaattc accttgctaa tccttatgcc
     6541 ttttgggggg gaagggtaaa tgagaatata aatgggttaa ttaaacaaca ttctttaaga
     6601 ggaactgact ttaataaaat ttttaaccgg aagataaatt tttttgtaaa tggaataaat
     6661 aattgccccc gaaaaactcg gaatataaaa ccccctaatt aattatttaa aggaataaga
     6721 gtttatttgc tcgtagagta attatgttcc attaatatgc taaattgctc agtaaagtta
     6781 ttaacttttt ttcaacgcca gtgaataagt ctgttttatt aaataaggag ttcaggtgtt
     6841 tatttcaaaa gaattatcaa gctttattgc tgtagctaaa aataaatcaa taaataaagc
     6901 aacaacggaa ctttttatta cacagtcacc gatttgccga agcttaaaaa aactggaatt
     6961 ttcattaggt gtaaaactct ttgtaagaaa aagccaggga ttggctttaa cagaagaagg
     7021 agaatgctta tataataaag ttttgtcaat ttgtgaacgg cttcaagaaa tagaaggtga
     7081 ctacaagaat gaaaaatcaa catacaatga tctatatcaa aaaaatatat gaggttctaa
     7141 tataataagc ggttctagat aactaaagtc atctattacc aatatctgta tactcagtga
     7201 aagttttgtc ttctaggtaa gttttcataa tagtgacata ccttattttt tcatctgggt
     7261 gtctctcatc ctgaagaatg ggtgatatag ttgaaataac tcagttcgtt ttgtggttta
     7321 cgatttttgg cggatcacaa aaatagggct gagtcccttc aagtgagttt tgaaatatac
     7381 aattataccc ttcatccttt aagttgttgc tttgttggct gttttcattc acctcagtca
     7441 catagttatc tatgctcctg aggattcgtt cacttgccgt cgtgctgcaa ctcgaaatct
     7501 attgggtata tatgtaattt tcatattctg tttcatttta acggtattat ttaaatattt
     7561 atatttagaa cattaggtta ttagaataga taattttagg taaatgtaat taattcaacc
     7621 atcacggaga gtttatcatt ttgcccaatg agggcaggag gtacggattt tcattagcac
     7681 ttatcgaagc ggatatatgt ttgtgactac attgtcagaa aatttcattc atcaacttga
     7741 attagaacag tgtccatata acttatttag aataaaggaa ggaaattaat cttagataca
     7801 atgataaggg aaataaaata gaaagtagag ttaattgtat taataaggtg ctggagcttt
     7861 acttacaaaa tatcaatgat ttttggtttc gtataagtaa ttaatttaga gtaatgaaaa
     7921 attatttgtg ggttaaattt tatttattga aaattcattg gtttatttta tgaaatagga
     7981 aggtaatagc ggtattattc ggttgaaata accgtataga taaatagtag gatttagata
     8041 aattatttca tatatgacaa tttttactat tatatggggt ttgtaagtcg accgatgccc
     8101 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     8161 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
     8221 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
     8281 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
     8341 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
     8401 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
     8461 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     8521 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg
     8581 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     8641 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     8701 acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg
     8761 gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat
     8821 ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct
     8881 ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt
     8941 gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct
     9001 gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa
     9061 gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat
     9121 gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct
     9181 gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt
     9241 ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca
     9301 tcggtatcat tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac
     9361 aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg
     9421 agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc
     9481 acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     9541 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     9601 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     9661 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     9721 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     9781 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9841 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9901 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9961 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10021 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10081 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10141 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10201 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10261 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10321 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10381 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    10441 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10501 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10561 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10621 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10681 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10741 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10801 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10861 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10921 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10981 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11041 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11101 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11161 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11221 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11281 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11341 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11401 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11461 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11521 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11581 aatactcat
//
