LOCUS       sequence-454362-       12547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bichromatic fluorescent reporter of THRA isoform 1 and isoform 2 
            splicing in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12547)
  AUTHORS   Graceffo E, Pedersen E, Rosario M, Krude H, Schuelke M
  TITLE     Bichromatic Splicing Detector Allows Quantification of THRA1 and 
            THRA2 Splicing Isoforms in Single Cells by Fluorescent Live-Cell 
            Imaging.
  JOURNAL   Int J Mol Sci. 2024 Dec 17;25(24):13512. doi: 10.3390/ijms252413512.
  PUBMED    39769274
REFERENCE   2  (bases 1 to 12547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2024-12-17"; volume: "25(24):13512. doi"; pages: " 
            10.3390/ijms252413512"
COMMENT     Sequence Label: pCMV-THRA-RFP-EGFP
FEATURES             Location/Qualifiers
     source          1..12547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      616..625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3378..4091
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             complement(4337..4348)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             join(8013..8015,8016..8729)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8055..8076)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8316..8335)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    9336..9456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9373..9392)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9426..9445
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9463..9918)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9600..9621)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9812..9831
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9945..10049
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10051..10408
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10051..10242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10074..10094)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10259..10394
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10443..11237
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10443..11237
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10497..10516)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11107..11126
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11425..11444)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11469..11516
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11845..12433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12334..12353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgccac catgggtccc gatgacattg gccagtcacc cattgtctcc
      661 atgccggacg gagacaaggt ggacctggaa gccttcagcg agtttaccaa gatcatcacc
      721 ccggccatca cccgtgtggt ggactttgcc aaaaaactgc ccatgttctc cgaggtgagt
      781 ggcagaagtg gggagagatg tgatgctgtg ctggagggaa acctgctccc ccggtcaccc
      841 agtacaggct catctgaggt tctctggaca aggagcaatt cctgtaggtc aacatacaca
      901 gataacatac acagacacac acacacacat gcatacacat acacccagca cacaggcaca
      961 catgcagaca catacacgga cacacacaca cacctagcat acagatacgt acacagacac
     1021 acatacacac acacaaacac acaggcacac acacacagca tacagacaca cgccaaggtc
     1081 tggctgctct aagaaatcag ccggctccaa gtgctcccct ccacagcccc tcatccttca
     1141 agtctatgcg gccacccaac tccaggcagt gctggaggga aacctgctcc cccggtcacc
     1201 cagtacaggc tcatctgagg ttctctggac aaggagcaat tcctgtaggt caacatacac
     1261 agataacata cacagacaca cacacacaca tgcatacaca tacacccagc acacaggcac
     1321 acatgcagac acatacacgg acacacacac acacctagca tacagatacg tacacagaca
     1381 cacatacaca cacacaaaca cacaggcaca cacacacagc atacagacac acgccaaggt
     1441 ctggctgctc taagaaatca gccggctcca agtgctcccc tccacagccc ctcatccttc
     1501 aagtctatgc ggccacccaa ctccaggcag cctgaaagtt ctcattttcc agagcactag
     1561 tgctggagac aggctgccct actagccatg tgacctgggg taagttacct ccatcccctg
     1621 tgcctcagtg ttcccatctg gagtgggctg gggataatga tgggtgctaa ctcacaggca
     1681 taattttttt tttctttttt gagacagatt ttcgttctgt cacccaggct ggagtgcagt
     1741 gacgcgatct gggctcactg caacctccgc ctcctgagtt caagcaattc tcctgcctta
     1801 gcctcctgag tagctgggac tacaggcatg tgccaccacg cccaactaat tttgtatttt
     1861 tagtagagat gggtttcacc atgtatgttg gccaggctgg tctcgaactc ctgacctcaa
     1921 gtgatccacc cgccttggcc tcccatagtg ctggcattat agtcgtgagc cactgcgccg
     1981 ggcctcacag gcataactgt gaggcttaaa tggaataggg catgcacatg gcccaataca
     2041 aggtcagccg ttcagagtcc atgggggcct tgcgggcctc acggctcccg taggacactc
     2101 taggggagac tcaaggacgc ggggaggagt atgctgagtg ctcctgtggc cctgccgctc
     2161 cacagctgcc ttgcgaagac cagatcatcc tcctgaaggg gtgctgcatg gagatcatgt
     2221 ccctgcgggc ggctgtccgc tacgaccctg agagcgacac cctgacgctg agtggggaga
     2281 tggctgtcaa gcgggagcag ctcaagaatg gcggcctggg cgtagtctcc gacgccatct
     2341 ttgaactggg caagtcactc tctgccttta acctggatga cacggaagtg gctctgctgc
     2401 aggctgtgct gctaatgtca acaggtacct gctgattagt cgggagggct cagaagcttc
     2461 caggggtccc agagattggc tggaccctgg gcctgttgct caactccctc ctgaatcttc
     2521 ttctgggcta cctctctgtc acctgggcca tcccctatcc cctgttcctt gctctgacac
     2581 acacttctca ctcgttcccc aggcccatcc cttgcctccc catccctgct ctgtgccttt
     2641 tcctgtcttt gtctccttcc ttctcgctgc cctgtccctc tgtcactcac attccccttt
     2701 gttccccact ccccactttg ttctcagcca ccttccatcc cctcatccct cctctgtctc
     2761 ccagtcccat ctcttacccc tcagtcccct ccctaacccc atctgcaccc cgcctctctg
     2821 accaccaacc tcagtcctcc ccactccaag gctcctctct ctcccccagc ccccggcctg
     2881 tccctctgtc ctccttgcat tgccccctcc ccccagtacc cccctgcccc tctccacctc
     2941 ccagctgcct ccctctccct gtgcttctcc tgtccacgtc tctcaggggg agcttctccc
     3001 ctcccctccc ccagcctctc tgcctctatc tcccctctag tcctttcttc ccacgtcccc
     3061 acacctcacc ctccccatct ccaggccttc ggccagcccc tcgcccctca cgcccctctt
     3121 ccctcacaga ccgctcgggc ctgctgtgtg tggacaagat cgagaagagt caggaggcgt
     3181 acctgctggc gttcgagcac tacgtcaacc accgcaaaca caacattccg cacttctggc
     3241 ccaagctgct gatgaaggtg actgacctcc gcatgatcgg ggcctgccac gccagccgct
     3301 tcctccacat gaaagtcgag tgccccaccg aactcttccc cccactcttc ctcgaggtct
     3361 ttgaggatca ggaagtcatg gtgtctaagg gcgaagagct gattaaggag aacatgcaca
     3421 tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg
     3481 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc
     3541 tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaga accttcatca
     3601 accacaccca gggcatcccc gacttcttta agcagtcctt ccctgagggc ttcacatggg
     3661 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
     3721 aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca tccaacggcc
     3781 ctgtgatgca gaagaaaaca ctcggctggg aggccaacac cgagatgctg taccccgctg
     3841 acggcggcct ggaaggcaga agcgacatgg ccctgaagct cgtgggcggg ggccacctga
     3901 tctgcaactt caagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg
     3961 gcgtctacta tgtggaccac agactggaaa gaatcaagga ggccgacaaa gagacctacg
     4021 tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca
     4081 aacttaattg aagcctcagg cggccagagg gtgtgcggag ctggtgggga ggagcctgga
     4141 gagaaggggc agagctgggg gctgagggag acccccccac accccttctc tccttcctct
     4201 cgtccttgga tagattcagc tcccacacac acacccgcac tgcccaggtc cctcctcaga
     4261 cctccagccc tgggacaggg caaacaactg aacttgctat ggaaaggaca gtgtgggagg
     4321 ctgggggagc tgtgtcctgc agttcccagg accccatcct ctcagaaggt aggggaaggg
     4381 cgggaggatt gagaagggac aagccacctt gaccgtaggg gaaggaggaa tgtgggctgg
     4441 gggaagatgc cctcaactca ccccctacac acacatgaga gagagccccc acccagttcc
     4501 ttggcctagg tctcccctcc aggctgaggg cctctctact tccccagatg cctgggtgca
     4561 aagaacggct tggcttggct cctcctctgg aggttaaaat ttatagtcat tctaactgca
     4621 ctttggaaac caagcagggg gagaagacaa atgaagaaaa actagacaga gagaaaaata
     4681 caaaaaagag agagcgagcg atagagagag atgatattaa gttattaact gaggctgacc
     4741 agaggggagg accccccctt tgccacccca tgcactttgc gagctgcccc ttcttccccc
     4801 acatcagaga gaaatgcccc cacaccagag ccccaagggt agggctgccg atcagagctg
     4861 tgagttaaca caacagggtc ctggccaccc cccttgccat accccgccct ctgtgcccct
     4921 ttggcacccc ccaaccctag tatgtcctct gctttctgcc actcgtgccc gctgagttcc
     4981 atctacctct catgctggat gccagctggc ccctcaccag cctgccctgc tgcccacaca
     5041 gctccccttt aagtgcccag ccctaggggc ctctccccat ccacctcctc cttccacacc
     5101 ttggcaccca cccaggaaaa aactgtggct ccaactccta ccctccttat atcctgcagt
     5161 attagctaga aaccggatgc tgctgcattg gttgggtcgg gggtaggagg gctgtgcgtg
     5221 agcgtctccc agaaccctcc acccctttag cactctgttt ccctgtttcc catctgtctt
     5281 ctgggctctc tctaacctct gccctcctcc ccagctccta ctcactgcac taactctggg
     5341 cgggcaggta ccagaatggg ggtgtttaga taagtgacac ttagttgggg ccccagggga
     5401 ggggtggtcg gtgctccttt ttctttaaag ctcttttggc cagctgcccc tctgccccgg
     5461 gaccccttcc cctcttcttt tctctaattc agggactttg gcttgagccc ctctcgccct
     5521 cctggtgagg gtgctctgtt ggtctgcact tgggtgagct gtcctcctcc ccctccctgt
     5581 ggctgccgcc cactcctcag gggagagagg gagagaggag gtggtcctcc catgggagca
     5641 ggaggtgggg tgggacagag cagaccagag ggtgctgggc tcagggctgc atgtcggcag
     5701 ggatggatgg gtggacacag atgcccccgg aagccatggg gattggggca gggggtgggg
     5761 ggaggggtgc cagggcttcc tgcgctttgc actattgggg caaaaatgtc ttaagcagtg
     5821 gggaacctgc caccccaccc tgaccctcag cctgccacag ccccctacac acacacacac
     5881 acacacacac acacacacac acacggacat gcacacacgg acatgggaag gcaatgctat
     5941 gctgcccgtc agggcactgt ccttccccaa tcgttcaggt gttccaacag gggtggaggg
     6001 cctggaggag cacccgctgt cacctgtgca tgtgcctgtc ccaccctgct ggggcctcgg
     6061 gctgcccgcg ctgtctttgt ctctatcctc tctctctctc ctggagtact gaatcctgta
     6121 ttactccagt cccatgggta ggccctcttg taccttccta ccacgcctgg gggcccagtg
     6181 gaattcctga cctgtctgct gtcttcccca tccctccatc cccaccccaa cccctccatc
     6241 cccattctca gccatggaca cggcagagaa aaggccttga agagccttag cctcttatag
     6301 gcacttggga ctcccccacc ctccccaatc atatgagctg tgatcccatc ctccccggac
     6361 ccctcccttc cccctcggtg atgtggggtg tatgtgtgcg ttcctctgcg tgaatgtgtg
     6421 agtgagtaca catggtaggg gaggagccag ggcgactcag gagtgccacg cacctgctct
     6481 agagcggcag ctgtcccagt ccctgctgtg gaagtggggg acagggccct ctcctggggg
     6541 ccattggtgc taatgagggg gatggccttg atgtgggtgc tcagcatgcc tcccccagta
     6601 ttggcccggg gcactagggc aggcagggtg gagcagcagg tgcagtatcg gtggggggac
     6661 gggttgctgg gaataggacg agggggccag gccaggacaa ggggttgcca gagccatgac
     6721 cactaccccg cccccaccac ccgcctctcc atctcagtat tgcccctccc atcactcata
     6781 acacacatac ctcatccagc ctcctccctg ctcagacttg gggagggtgg gggaggagcc
     6841 cctacccctt cccctggtgg cgggtctgca gggctgggag agggcagggc gttgtgagag
     6901 agagaccgtc cataaggagg acagtaactc ctgccctggg aactcctggg cgggggggag
     6961 ggggacactg cccagaggcg ctactgaatg tatgagaagc tggtgctggg ctggggggag
     7021 gggggttgtg gccagaaaca gggaaggagg taggtctctt ccccagggag gggtgggacc
     7081 ggcaggggtg acttgagggg ctcctactcc tgccccacgt tgacaatcag tatgtctgtt
     7141 atgtgcgatt tttcaccccg ttgtgttttg ggtcaggatt ttaaagaaag atatttttat
     7201 ggtaattgtt gctcgtctat tttactatat atttatgtaa taaatatatg atgaaaataa
     7261 cccccttggg cacccccctt agacttgtgt gctgtttccc tatatcctcc catctgctgg
     7321 cagagtaccc accccacaaa ctgaccagat ggagaggtgg ccccccccag ccttggcagt
     7381 atttccaccc caccccccaa acgagcacac accacagaag ccagctcagc tgtgaactat
     7441 tggatttgag acaggaacag aacaaatcag agggccaggg gagggttgtg ggggagacag
     7501 agtggtttaa ataggggagg aggggaagtt cggtgatggg ggagggaggc aggtatttac
     7561 aagaaggctc agggggccag aggctcatct tggaatattt tataacaata taaataagat
     7621 tctggtttgc ttttcctttt cgtctcgtaa aggagagaga agtgcagagt tcgattctgt
     7681 acaagggggc agcggcagaa ggccggccgg gcgggtcact gggcgtccac ccggaaggac
     7741 agcagcttct cggaatgcat gttgttcagg gtccgcaggt ccggcagctt gagcagcagc
     7801 ttggtgaagc gggaagtctc caagggccgg ttcttcagca ccagagcccg aagagcccgc
     7861 agcagcgtct cctggagctg ctccaccgaa gcggaattct ccatgcccga gtggtctgtg
     7921 gggaagacga cagcagtgag gcggactccc cgagctcctc tgaggaggaa ccggaggtct
     7981 gcgaggacct ggcaggcaat gcagcctctc ccgtgagcaa gggcgaggag ctgttcaccg
     8041 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     8101 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     8161 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     8221 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     8281 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     8341 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     8401 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     8461 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     8521 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     8581 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     8641 accccaacga gaagcgcgat cacatggtcc tgaaggagtt cgtgaccgcc gccgggatca
     8701 ctctcggcat ggacgagctg tacaagtaaa gccccccaga aggccgatgg ggaaggagaa
     8761 ggagtgccat accttctccc aggcctctgc cccaagagca ggaggtgcct gaaagctggg
     8821 agcgtgggct cagcagggct ggtcacctcc catcccgtaa gaccaccttc ccttcctcag
     8881 caggccaaac atggccagac tcccttgctt tttgctgtgt agttccctct gcctgggatg
     8941 cccttccccc tttctctgcc tggcaacatc ttacttgtcc tttgaggccc caactcaagt
     9001 gtcacctcct tccccagctc ccccaggcag aaatagttgt ctgtgcttcc ttggttcatg
     9061 cttctactgt gacacttatc tcactgtttt ataattagtc gggcatgagt ctgtttccca
     9121 agctagactg tgtctgaatc atgtctgtat ccccagtgcc cggtgcaggg cctggcatag
     9181 agtaggtact ccataaaagg tgtgttgaat tgaagcggcc gcgactctag atcataatca
     9241 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     9301 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     9361 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt cactgcattc
     9421 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     9481 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     9541 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     9601 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     9661 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     9721 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     9781 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     9841 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     9901 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     9961 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    10021 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
    10081 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    10141 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
    10201 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
    10261 cccgccccta actccgccca gttccgccca ctctccgccc catggctgac taattttttt
    10321 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    10381 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
    10441 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
    10501 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
    10561 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
    10621 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
    10681 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
    10741 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
    10801 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
    10861 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
    10921 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
    10981 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
    11041 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
    11101 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
    11161 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
    11221 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
    11281 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
    11341 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
    11401 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
    11461 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
    11521 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
    11581 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
    11641 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
    11701 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    11761 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    11821 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    11881 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    11941 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    12001 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    12061 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    12121 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    12181 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    12241 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    12301 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    12361 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    12421 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    12481 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    12541 catgcat
//
