LOCUS       sequence-45447-e        1535 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Dittmer PJ, Miranda JG, Gorski JA, Palmer AE
  TITLE     Genetically encoded sensors to elucidate spatial distribution of 
            cellular zinc.
  JOURNAL   J Biol Chem. 2009 Jun 12;284(24):16289-97. Epub 2009 Apr 10.
  PUBMED    19363034
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2009-06-12"; volume: "284(24)"; pages: "16289-97. 
            Epub 2009 Apr 10"
COMMENT     Sequence Label: pcDNA-ZifCY1
FEATURES             Location/Qualifiers
     source          1..1535
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(15..17,18..20,21..533)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             join(810..812,813..815,816..1526)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 cgagctcgga tcccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
       61 tggtcgagct ggacggcgac gtaaacggcc acaggttcag cgtgtccggc gagggcgagg
      121 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      181 tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc
      241 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      301 agcgtaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      361 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      421 acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg
      481 acaagcagaa gaacggcatc aaggcccact tcaagatccg ccacaacatc gaggacggca
      541 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      601 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
      661 gcgatcacat ggtcctgctg gagttcgtga ccgccgcccg catgcatgaa cgcccatatg
      721 cttgccctgt cgagtcctgc gatcgccgct tttctcgctc ggatgagctt acccgccata
      781 tccgcatcca cacaggccag aaggagctca tggtgagcaa gggcgaggag ctgttcaccg
      841 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      901 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      961 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgatgt
     1021 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1081 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1141 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1201 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1261 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1321 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1381 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag
     1441 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1501 ctctcggcat ggacgagctc tacaagtaag aattc
//
