LOCUS       sequence-45450-e        1534 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Dittmer PJ, Miranda JG, Gorski JA, Palmer AE
  TITLE     Genetically encoded sensors to elucidate spatial distribution of 
            cellular zinc.
  JOURNAL   J Biol Chem. 2009 Jun 12;284(24):16289-97. Epub 2009 Apr 10.
  PUBMED    19363034
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2009-06-12"; volume: "284(24)"; pages: "16289-97. 
            Epub 2009 Apr 10"
COMMENT     Sequence Label: pcDNA-ZifCY2
FEATURES             Location/Qualifiers
     source          1..1534
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(14..16,17..19,20..532)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             join(809..811,812..814,815..1525)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 gagctcggat cccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
       61 ggtcgagctg gacggcgacg taaacggcca caggttcagc gtgtccggcg agggcgaggg
      121 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      181 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc
      241 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      301 gcgtaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      361 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      421 catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga
      481 caagcagaag aacggcatca aggcccactt caagatccgc cacaacatcg aggacggcag
      541 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      601 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
      661 cgatcacatg gtcctgctgg agttcgtgac cgccgcccgc atgcatgaac gcccatatgc
      721 tcaccctgtc gagtcccacg atcgccgctt ttctcgctcg gatgagctta cccgccatat
      781 ccgcatccac acaggccaga aggagctcat ggtgagcaag ggcgaggagc tgttcaccgg
      841 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      901 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      961 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg
     1021 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     1081 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     1141 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     1201 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     1261 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     1321 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     1381 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga
     1441 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     1501 tctcggcatg gacgagctct acaagtaaga attc
//
