LOCUS       sequence-45454-e        2370 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Palmer AE, Giacomello M, Kortemme T, Hires SA, Lev-Ram V, Baker D, 
            Tsien RY
  TITLE     Ca2+ indicators based on computationally redesigned 
            calmodulin-peptide pairs.
  JOURNAL   Chem Biol. 2006 May;13(5):521-30.
  PUBMED    16720273
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2006-05"; volume: "13(5)"; pages: "521-30"
COMMENT     Sequence Label: pcDNA-4mtD3cpv
FEATURES             Location/Qualifiers
     source          1..2370
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..81
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             103..177
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             205..279
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             301..375
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      397..406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(403..405,406..408,409..921)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             1627..2361
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 aagcttatgt ccgtcctgac gccgctgctg ctgcggggct tgacaggctc ggcccggcgg
       61 ctcccagtgc cgcgcgccaa gatccattcg ttgggggatc ccatgtccgt cctgacgccg
      121 ctgctgctgc ggggcttgac aggctcggcc cggcggctcc cagtgccgcg cgccaagatc
      181 cattcgttgg ggaagcttgc caccatgtcc gtcctgacgc cgctgctgct gcggggcttg
      241 acaggctcgg cccggcggct cccagtgccg cgcgccaaga tccattcgtt gggggatccc
      301 atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca
      361 gtgccgcgcg ccaagatcca ttcgttgggg aagcttgcca ccatggtgag caagggcgag
      421 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      481 aggttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      541 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      601 tggggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      661 tccgccatgc ccgaaggcta cgtccaggag cgtaccatct tcttcaagga cgacggcaac
      721 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      781 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      841 atcagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggcccacttc
      901 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      961 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1021 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1081 gccgcccgca tgcatgacca actgacagaa gagcagattg cagagttcaa agaagccttc
     1141 tcattattgg acaaggatgg ggacggcacc atcaccacaa aggaacttgg caccgctctg
     1201 aggtcgcttg gacaaaaccc aacggaagca gaattgcagg atatgatcaa tgaagtcgat
     1261 gctgatggca atggaacgat ttactttcct gaatttctta ctatgatggc tagaaaaatg
     1321 aaggacacag acagcgaaga ggaaatccga gaagcattcc gtgtttttga caaggatggg
     1381 aacggctaca tcagcgctgc tgaattacgt cacgtcatga caaacctcgg ggagaagtta
     1441 acagatgaag aagttgatga aatgataagg gaagcagata tcgatggtga tggccaagta
     1501 aactatgaag agtttgtaca aatgatgaca gcaaaggggg ggaagaggcg ctggcagaaa
     1561 acaggccatg ctgtccgagc attcggaaga ctgaagaaga tctccagctc cggggcactg
     1621 gagctcatgg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1681 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     1741 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1801 actctcggca tggacgagct gtacaagggt ggcagcggtg gcatggtgag caagggcgag
     1861 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1921 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1981 ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc
     2041 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     2101 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     2161 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     2221 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     2281 aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     2341 aagatccgcc acaacatcga gtaagaattc
//
