LOCUS       sequence-454875-        3463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPMS0CM0041
FEATURES             Location/Qualifiers
     source          1..3463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1507..1523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1507..1523)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1520..1542)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1695..2283)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1775..1794)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2416..2510)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2531..3190)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3105..3124
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3191..3293)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3380..3402
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3394..3411
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3395..3411
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taggtctcta atggacgtgt tatatggctc ccttatgtcc gtctttgtaa tcttcgccac
       61 cttggcactt cacttcctat tctacaagaa caaccggaaa ggtgtccacc caccgggcgg
      121 aaaaggatgg ccgtttttgg gagagacagt ggagtttctg tccacgggtt ggaatggcca
      181 tcctgaaaaa ttcgtatttg atcgaatggc caaatactcc tcaaatatct tcaagacatc
      241 cttgttagga tacaagaccg cagtattttg cgggccagcc ggcaacaaat tcttgttctc
      301 gaacgagtac aaacttgtac gaatgtgggt tccggattat gttaacaagt tgttcccatc
      361 taaatcaagt gcggccgatg aagccatgaa aatgaggaaa tcttttccat tgttcctaaa
      421 acccgaggcg ttgcaaaaat acataggtgt aatggatgag atcacgggaa aatattttac
      481 aacttcatgg gaaaaccatg aagttgtcgc cgtctatcct cttataaaac aattcgcctt
      541 tgtgatcgca tctcggttgt ttattagtgt agaagatcct gaccacattg caaagtttgt
      601 aacccctttt gatgccttag tttcgggcat cttttcgata ccgatagatt tcccgggaac
      661 acaatttaac aaagcggtta aaggttcgaa gcatataagg aaagagatta tcgatataat
      721 caaacaaaga aaggttgatt tgaaggaagg gaaggcttct cctaagcaag atgttttgtc
      781 acatatgctt gttacaaatg atgatgatga ggatgctatg accgattcag atattgctaa
      841 taaattgata gctttattaa ttggtgggca tgatactatt gctagtacat gcacttctat
      901 tgtgagatat cttgccgagc ttccccatat ttacgaagag gtttataaag aacaaatgga
      961 agtggcaaag tcaaaagggc caggtgaagt attaaactgg gaagatatac ataagatgaa
     1021 atattcatgg aatgttgcat gtgaagtgtt gaggctttcc cctcctttcc aaggaacatt
     1081 tagagaagta cttgcagata tcacctttaa tggtttctcc atcccaaagg gatggaagat
     1141 ttattggagc acaaatggag tacataagaa ccccgaatac tttcccgagc ccctaaaatt
     1201 tgacccgtca cgatatgagg gagccgggcc agctccatac acatatgtcc cattcggagg
     1261 aggggctcgg atgtgtcccg gaaaagagtt tgctcgatta gagatactcg ttttcatgca
     1321 ccatattgtg aagagattcc gatgggaaaa aaccataaat gatgaaaaga tcgttgttga
     1381 tcctatgccc gttcctgcca aagggctccc tgttcgtctt tacccgcata aaaccggttc
     1441 gagagaccta aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt
     1501 gtcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat
     1561 tccgcgctat ccgacaatct ccaagacatt aggtggagtt cagttcggcg tatggcatat
     1621 gtcgctggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1681 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1741 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1801 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1861 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1921 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1981 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2041 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2101 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     2161 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2221 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2281 caagaagatc ctttgatctt ttctacgggg tctgacgctc tattcaacaa agccgccgtc
     2341 ccgtcaagtc agcgtaaatg ggtagggggc ttcaaatcgt ccccccatac gatataagtt
     2401 gttactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     2461 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     2521 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     2581 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     2641 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     2701 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     2761 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     2821 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     2881 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     2941 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
     3001 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     3061 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     3121 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     3181 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     3241 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc
     3301 attgatacct gaaacaaaac ccatcgtacg gccaaggaag tctccaataa ctgtgatcca
     3361 ccacaagcgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tcatgcataa
     3421 tccgcacgca tctggaataa ggaagtgcca ttccgcctga cct
//
