LOCUS       sequence-454881-        3090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPCT0CM0208
FEATURES             Location/Qualifiers
     source          1..3090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1134..1150)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1134..1150)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1147..1169)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1322..1910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1402..1421)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2043..2137)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2158..2817)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2732..2751
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2818..2920)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3007..3029
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3021..3038
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3022..3038
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ataggtctct aatggaggga gagatgaaaa atatgatatt tgtatgggca accgcgtttg
       61 gatctctctt catctgccac aagatcacca aagttactgc accaggtaca agtcgcttat
      121 gcgcattcat ccctgtggtt tgcattttct tatacctccc cctccgactt actactgttc
      181 atttcgccgg cacaacctct ttcttcatct cttggcttgc aaacttcaaa ttgcttttgt
      241 ttgctttcaa taaaggccct ctgtattcaa atccacccct tcctctctcc aaattcatcc
      301 tcactgcttc tgtacccatt caaatttcat ctcatagatc taattctgat cccaaaaaat
      361 caccgacggc gatcaaatac tccctcaagt tcatggtgtt tgttctgttg cttaaagtgt
      421 atgcatacga cgatcaattt catcgtctta tcaaattgac tctgttttgt gttcatttat
      481 atgtggctct agacctaggt ttagccatgg ttgcatacct ggctcgatcc atcactggat
      541 tcgacctcga accacagttc gacgaacctt acctcgctac atctctacag gatttttggg
      601 gaaagaggtg gaatctaatg gtcacgggta tactacaccc tgccgtgtac ctacccgtca
      661 gagcaatttc cggcaggttt ttgaccaaaa atttggccgc aattccagct cttatgtcat
      721 cattcatcgt ttccgggttg atgcacgagt tgatttttta ttacattgga cgattgaaac
      781 ccacatggga agtaacgggg ttcttcgtca ttcatggtgt gctgctgagt atggaagtgg
      841 tggtaaaaag gtcggtagaa ggaagattcc atctgccagc tgtggtttcc aggccgatgg
      901 cgttgggttt ggtgatcgcc accagcttct ggttattctt tccgccgttc ctgcggtttg
      961 attcggaagt ccgattatgt gaagaatttc tcgccttcat ggaacttgtc agtcgtggac
     1021 ggtttgttgg cccaaatgac gtcccttgcc cttactttag aagcttcgag agacctaagg
     1081 ctaggtggag gctcagtgat gataagtctg cgatggtgga tgcatgtgtc atggtcatag
     1141 ctgtttcctg tgtgaaattg ttatccgctc agagggcaca atcctattcc gcgctatccg
     1201 acaatctcca agacattagg tggagttcag ttcggcgtat ggcatatgtc gctggaaaga
     1261 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1321 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1381 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1441 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1501 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1561 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1621 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1681 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1741 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1801 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1861 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1921 tgatcttttc tacggggtct gacgctctat tcaacaaagc cgccgtcccg tcaagtcagc
     1981 gtaaatgggt agggggcttc aaatcgtccc cccatacgat ataagttgtt actagtgctt
     2041 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     2101 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     2161 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     2221 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     2281 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     2341 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     2401 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     2461 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     2521 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     2581 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     2641 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     2701 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     2761 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     2821 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     2881 ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt gatacctgaa
     2941 acaaaaccca tcgtacggcc aaggaagtct ccaataactg tgatccacca caagcgccag
     3001 ggttttccca gtcacgacgt tgtaaaacga cggccagtca tgcataatcc gcacgcatct
     3061 ggaataagga agtgccattc cgcctgacct
//
