LOCUS       sequence-455016-        7669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adenoviral helper plasmid containing E2A and L4-22k/33k and VA RNA I
            genes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Doshi J, Couto E, Staiti J, Vandenberghe LH, Zabaleta N
  TITLE     E2A, VA RNA I, and L4-22k adenoviral helper genes are sufficient for
            AAV production in HEK293 cells.
  JOURNAL   Mol Ther Methods Clin Dev. 2024 Nov 13;32(4):101376. doi: 
            10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12.
  PUBMED    39670178
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2024-11-13"; volume: "32(4):101376. doi";
            pages: " 10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12"
COMMENT     Sequence Label: JD136
FEATURES             Location/Qualifiers
     source          1..7669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        254..272
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     254..271
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5631..5650)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5632..5650)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5742..5846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5847..5915,5916..6707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6065..6084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6905..7493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7394..7413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7647..7664
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt
       61 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg
      121 cattacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag
      181 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctgcagg
      241 aaggtttaaa cgcatttagg tgacactata gaagtgtgta tcgctcgagg gatccgaatt
      301 cgaagtcttg gtacggagcg agaccggagc gagacgggag tcgtcttcgc tttccagatc
      361 tgataacttg tgaagacgac catcgtctca ccatggtctc accattcctg tagacttctt
      421 aattaacggt atgcagggcg accgcaccct gtgacgaaag ccgcccgcaa gctgcgcccc
      481 tgagttagtc atctgaactt cggcctgggc gtctctggga agtaccacag tggtgggagc
      541 gggactttcc tggtacacca gggcagcggg ccaactacgg ggattaaggt tattacgagg
      601 tgtggtggta atagccgcct gttccaggag aattcggttt cggtgggcgc gtattccgtt
      661 gacccgggat atcatgtggg gtcccgcgct catgtagttt attcgggttg agtagtcttg
      721 ggcagctcca gccgcaagtc ccatttgtgg ctggtaactc cacatgtagg gcgtgggaat
      781 ttccttgctc ataatggcgc tgacaacagg tgctggcgcc gggtgtggcc gctggagatg
      841 acgtagtttt cgcgcttaaa tttgagaaag ggcgcgaaac tagtccttaa gagtcagcgc
      901 gcagtattta ctgaagagag cctccgcgtc ttccagcgtg cgccgaagct gatcttcgct
      961 tttgtgatac aggcagctgc gggtgaggga tcgcagagac ctgtttttta ttttcagctc
     1021 ttgttcttgg cccctgctct gttgaaatat agcatacaga gtgggaaaaa tcctgtttct
     1081 aagctcgcgg gtcgatacgg gttcgttggg cgccagacgc agcgctcctc ctcctgctgc
     1141 tgccgccgct gtggatttct tgggctttgt cagagtcttg ctatccggtc gcctttgctt
     1201 ctgtgtggcc gctgctgttg ctgccgctgc cgccggtgca gtatgggctg tagagatgac
     1261 ggtagtaatg caggatgtta cgggggaagg ccacgccgtg atggtagaga agaaagcggc
     1321 gggcgaagga gatgttgccc ccacagtctt gcaagcaagc aactatggcg ttcttgtgcc
     1381 cgcgccatga gcggtagcct tggcgctgtt gttgctcttg ggctaacggc ggcggctgct
     1441 tggacttacc ggccctggtt ccagtggtgt cccatctacg gttgggtcgg cgaacgggca
     1501 gtgccggcgg cgcctgagga gcggaggttg tagccatgct ggaaccggtt gccgatttct
     1561 ggggcgccgg cgaggggaat gcgaccgagg gtgacggtgt ttcgtctgac acctcttcga
     1621 cctcggaagc ttcctcgtct aggctctccc agtcttccat catgtcctcc tcctcctcgt
     1681 ccaaaacctc ctctgcctga ctgtcccagt attcctcctc gtccgtgggt ggcggcggca
     1741 gctgcagctt ctttttgggt gccatcctgg gaagcaaggg cccgcggctg ctgctgatag
     1801 ggctgcggcg gcggggggat tgggttgagc tcctcgccgg actgggggtc caagtaaacc
     1861 ccccgtccct ttcgtagcag aaactcttgg cgggctttgt tgatggcttg caattggcca
     1921 agaatgtggc cctgggtaat gacgcaggcg gtaagctccg cattaggcgg gcgggattgg
     1981 tcttcgtaga acctaatctc gtgggcgtgg tagtcctcag gtacaaattt gcgaaggtaa
     2041 gccgacgtcc acagccccgg agtgagtttc aaccccggag ccgcggactt ttcgtcaggc
     2101 gagggaccct gcagctcaaa ggtaccgata atttgacttt cgttaagcag ctgcgaattg
     2161 caaaccaggg agcggtgcgg ggtgcatagg ttgcagcgac agtgacactc cagtagaccg
     2221 tcaccgctca cgtcttccat tatgtcagag tggtaggcaa ggtagttggc tagctgcaga
     2281 aggtagcagt ggccccaaag cggcggaggg cattcgcggt acttaatggg cacaaagtcg
     2341 ctaggaagtg cacagcaggt ggcgggcaag attcctgagc gctctaggat aaagttccta
     2401 aagttctgca acatgctttg actggtgaag tctggcagac cctgttgcag ggttttaagc
     2461 aggcgttcgg ggaaaatgat gtccgccagg tgcgcggcca cggagcgctc gttgaaggcc
     2521 gtccataggt ccttcaagtt ttgctttagc agtttctgca gctccttgag gttgcactcc
     2581 tccaagcact gctgccaaac gcccatggcc gtctgccagg tgtagcatag aaataagtaa
     2641 acgcagtcgc ggacgtagtc gcggcgcgcc tcgcccttga gcgtggaatg aagcacgttt
     2701 tgcccaaggc ggttttcgtg caaaattcca aggtaggaga ccaggttgca gagctccacg
     2761 ttggagatct tgcaggcctg gcgtacgtag ccctgtcgaa aggtgtagtg caatgtttcc
     2821 tctagcttgc gctgcatctc cgggtcagca aagaaccgct gcatgcactc aagctccacg
     2881 gtaacgagca ctgcggccat cattagtttg cgtcgctcct ccaagtcggc aggctcgcgc
     2941 gtttgaagcc agcgcgctag ctgctcgtcg ccaactgcgg gtaggccctc ctctgtttgt
     3001 tcttgcaaat ttgcatccct ctccaggggc tgcgcacggc gcacgatcag ctcactcatg
     3061 actgtgctca tgaccttggg gggtaggtta agtgccgggt aggcaaagtg ggtgacctcg
     3121 atgctgcgtt ttagtacggc taggcgcgcg ttgtcaccct cgagttccac caacactcca
     3181 gagtgacttt cattttcgct gttttcctgt tgcagagcgt ttgccgcgcg cttctcgtcg
     3241 cgtccaagac cctcaaagat ttttggcact tcgttgagcg aggcgatatc aggtatgaca
     3301 gcgccctgcc gcaaggccag ctgcttgtcc gctcggctgc ggttggcacg gcaggatagg
     3361 ggtatcttgc agttttggaa aaagatgtga taggtggcaa gcacctctgg cacggcaaat
     3421 acggggtaga agttgaggcg cgggttgggc tcgcatgtgc cgttttcttg gcgtttgggg
     3481 ggtacgcgcg gtgagaatag gtggcgttcg taggcaaggc tgacatccgc tatggcgagg
     3541 ggcacatcgc tgcgctcttg caacgcgtcg cagataatgg cgcactggcg ctgcagatgc
     3601 ttcaacagca cgtcgtctcc cacatctagg tagtcgccat gcctttcgtc cccccgcccg
     3661 acttgttcct cgtttgcctc tgcgttgtcc tggtcttgct ttttatcctc tgttggtact
     3721 gagcggtcct cgtcgtcttc gcttacaaaa cctgggtcct gctcgataat cacttcctcc
     3781 tcctcaagcg ggggtgcctc gacggggaag gtggtaggcg cgttggcggc atcggtggag
     3841 gcggtggtgg cgaactcaga gggggcggtt aggctgtcct tcttctcgac tgactccatg
     3901 atctttttct gcctatagga gaaggaaatg gccagtcggg aagaggagca gcgcgaaacc
     3961 acccccgagc gcggacgcgg tgcggcgcga cgtcccccaa ccatggagga cgtgtcgtcc
     4021 ccgtccccgt cgccgccgcc tccccgggcg cccccaaaaa agcggatgag gcggcgtatc
     4081 gagtccgagg acgaggaaga ctcatcacaa gacgcgctgg tgccgcgcac acccagcccg
     4141 cggccatcga cctcggcggc ggatttggcc attgcgccca agaagaaaaa gaagcgccct
     4201 tctcccaagc ccgagcgccc gccatcacca gaggtaatcg tggacagcga ggaagaaaga
     4261 gaagatgtgg cgctacaaat ggtgggtttc agcaacccac cggtgctaat caagcatggc
     4321 aaaggaggta agcgcacagt gcggcggctg aatgaagacg acccagtggc gcgtggtatg
     4381 cggacgcaag aggaagagga agagcccagc gaagcggaaa gtgaaattac ggtgatgaac
     4441 ccgctgagtg tgccgatcgt gtctgcgtgg gagaagggca tggaggctgc gcgcgcgctg
     4501 atggacaagt accacgtgga taacgatcta aaggcgaact tcaaactact gcctgaccaa
     4561 gtggaagctc tggcggccgt atgcaagacc tggctgaacg aggagcaccg cgggttgcag
     4621 ctgaccttca ccagcaacaa gacctttgtg acgatgatgg ggcgattcct gcaggcgtac
     4681 ctgcagtcgt ttgcagaggt gacctacaag catcacgagc ccacgggctg cgcgttgtgg
     4741 ctgcaccgct gcgctgagat cgaaggcgag cttaagtgtc tacacggaag cattatgata
     4801 aataaggagc acgtgattga aatggatgtg acgagcgaaa acgggcagcg cgcgctgaag
     4861 gagcagtcta gcaaggccaa gatcgtgaag aaccggtggg gccgaaatgt ggtgcagatc
     4921 tccaacaccg acgcaaggtg ctgcgtgcac gacgcggcct gtccggccaa tcagttttcc
     4981 ggcaagtctt gcggcatgtt cttctctgaa ggcgcaaagg ctcaggtggc ttttaagcag
     5041 atcaaggctt ttatgcaggc gctgtatcct aacgcccaga ccgggcacgg tcaccttttg
     5101 atgccactac ggtgcgagtg caactcaaag cctgggcacg cgcccttttt gggaaggcag
     5161 ctaccaaagt tgactccgtt cgccctgagc aacgcggagg acctggacgc ggatctgatc
     5221 tccgacaaga gcgtgctggc cagcgtgcac cacccggcgc tgatagtgtt ccagtgctgc
     5281 aaccctgtgt atcgcaactc gcgcgcgcag ggcggaggcc ccaactgcga cttcaagata
     5341 tcggcgcccg acctgctaaa cgcgttggtg atggtgcgca gcctgtggag tgaaaacttc
     5401 accgagctgc cgcggatggt tgtgcctgag tttaagtgga gcactaaaca ccagtatcgc
     5461 aacgtgtccc tgccagtggc gcatagcgat gcgcggcaga acccctttga tttttaaacg
     5521 gcgcagacgg caagggtggg ggtaaataat cacccgagag tgtacaaata aaagcatttg
     5581 cctttattga aagtgtctct agtacattat ttgcggccgc atgtgagtct ccctatagtg
     5641 agtcgtatta atttcgcggg cggaacccct atttgtttat ttttctaaat acattcaaat
     5701 atgtatccgc tcatgagtag caccaggcgt ttaagggcac ccgcggaacc cctatttgtt
     5761 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5821 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5881 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5941 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6001 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6061 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6121 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6181 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6241 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6301 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6361 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6421 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6481 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6541 aatctggagc cggtgagcgt ggatctcgcg gtatcattgc agcactgggg ccagatggta
     6601 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6661 atagacagat cgctgagata ggtgcgtcac tgattaagca ttggtaactg tcagaccaag
     6721 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6781 tgaagatcct ttacgtgccg atcaaagtct cattttcgcc aaaagttgtc atgaccaaaa
     6841 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6901 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6961 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7021 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7081 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7141 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7201 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7261 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7321 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7381 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7441 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7501 gcaatgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7561 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7621 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagc
//
