LOCUS       sequence-455545-        9313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human TGIF1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Henriques-Oliveira L, Altman AR, Kurochkin I, Ascic E, Halitzki E, 
            Matei A, Pertiga-Cabral D, Ulmert I, Holst S, Nair MS, Cunha PP, 
            Park S, Vergani S, Kharas MG, Yuan J, Lahl K, Rosa FF, Pires CF, 
            Pereira C-F
  TITLE     Anchored screening identifies transcription factor blueprints 
            underlying dendritic cell diversity and subset-specific anti-tumor 
            immunity
  JOURNAL   Immunity. 58, 1-20. October 2025.
REFERENCE   2  (bases 1 to 9313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            58, 1-20. October 2025."
COMMENT     Sequence Label: pFUW-TetO-TGIF1
FEATURES             Location/Qualifiers
     source          1..9313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(152..169)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    158..382
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      428..856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(515..534)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     725..746
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(865..885)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        870..1199
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1050..1185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1112..1131
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1247..1294
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1313..1687
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1817..1938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1854..1873)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1908..1927
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1987..2003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1987..2003)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2000..2022)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2011..2027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2035..2041,2042..2059,2060..2065))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2080..2101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2218..2235)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2389..2977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2469..2488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3148..3939,3940..4008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3771..3790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4009..4113)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4188..4207)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4379..4758
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4760..4958
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             4976..5156
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5203..5328
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5821..6054
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6239..6283
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6581..6698
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6755..6773
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6797..6815
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6839..6857
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6881..6899
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     gap             6911..7010
                     /estimated_length=100
     promoter        7032..7070
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7067..7091
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    8019..8607
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8072..8092)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8490..8501)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8610..8626)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8611..8627)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9132..9312
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
       61 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
      121 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
      181 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
      241 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
      301 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
      361 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
      421 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      481 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      541 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      601 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
      661 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
      721 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
      781 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
      841 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      901 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      961 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     1021 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     1081 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     1141 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     1201 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     1261 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     1321 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     1381 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     1441 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     1501 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     1561 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     1621 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     1681 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     1741 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     1801 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1861 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1921 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     1981 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2041 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     2101 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     2161 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2221 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2281 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2341 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2401 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2461 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2521 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2581 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2641 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2701 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2761 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2821 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2881 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2941 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3001 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3061 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3121 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3181 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3241 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3301 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3361 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3421 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3481 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3541 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3601 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3661 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3721 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3781 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3841 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3901 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3961 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4021 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4081 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4141 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     4201 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     4261 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     4321 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     4381 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     4441 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     4501 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     4561 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     4621 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     4681 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     4741 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     4801 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     4861 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     4921 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
     4981 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5041 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5101 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     5161 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     5221 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     5281 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     5341 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     5401 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     5461 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     5521 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     5581 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     5641 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     5701 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     5761 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     5821 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     5881 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     5941 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     6001 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     6061 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     6121 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     6181 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     6241 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     6301 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     6361 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     6421 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     6481 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     6541 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     6601 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     6661 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     6721 gagatccagt ttggttagat ctcgagttta ccactcccta tcagtgatag agaaaagtga
     6781 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     6841 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     6901 aagtgaaagt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgagctcggt
     7021 acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga
     7081 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
     7141 accgatccag cctccgcggc cccgaattca ccggtatgaa aggcaagaaa ggtattgttg
     7201 cagcatctgg cagtgagact gaggatgagg acagcatgga cattcccttg gacctttctt
     7261 catccgctgg ctcaggcaag agaaggagaa ggggcaacct acccaaggag tctgtgcaga
     7321 ttcttcggga ttggctgtat gagcaccgtt acaatgccta tccttcagag caagaaaaag
     7381 cgttgctgtc ccagcaaaca cacctgtcta cgctacaggt ctgtaactgg ttcatcaacg
     7441 cccgccgcag gctcctccct gacatgctga gaaaggatgg caaagatcca aatcagttca
     7501 caatttcccg ccgtggggcc aagatttctg aaacgagctc tgtggagtcc gtgatgggca
     7561 tcaaaaactt catgccagct ctagaggaga ccccgtttca ttcctgtaca gctgggccaa
     7621 acccaaccct agggaggcca ctgtctccta agccgtcatc cccgggatca gttttggctc
     7681 gtccatcagt gatctgccat accactgtga ctgcattgaa agatgtccct ttctctctct
     7741 gccagtcggt cggtgtggga caaaacacag atatacagca gatagcggcc aaaaacttca
     7801 cagacacctc tctcatgtac ccagaggaca cttgtaaatc tggaccaagt acgaatacac
     7861 agagtggtct tttcaacact cctcccccta ctccaccgga cctcaaccag gacttcagtg
     7921 gatttcagct tctagtggat gttgcactca aacgggctgc agagatggag cttcaggcaa
     7981 aacttacagc ttaattcgaa gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     8041 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     8101 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     8161 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     8221 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     8281 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     8341 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     8401 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     8461 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     8521 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     8581 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
     8641 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     8701 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     8761 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8821 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     8881 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     8941 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     9001 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     9061 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     9121 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9181 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9241 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9301 aaatctctag cag
//
