LOCUS       sequence-455560-        3208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AD-SYNZIP: alpha-NTD (P. aeruginosa) – SYNZIP17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Villegas Kcam MC, Tsong AJ, Chappell J
  TITLE     Uncovering the Distinct Properties of a Bacterial Type I-E CRISPR 
            Activation System.
  JOURNAL   ACS Synth Biol. 2022 Jan 25. doi: 10.1021/acssynbio.1c00496.
  PUBMED    35077145
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Jan 25. doi: 10.1021/acssynbio.1c00496."
COMMENT     Sequence Label: pJEC637
FEATURES             Location/Qualifiers
     source          1..3208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(282..373)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(421..1159)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        1218..1252
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     CDS             2146..2175
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2191..2208
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2264..2281)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2434..2480
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(2698..3208,1..281))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLKLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc
       61 gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc
      121 accgtaacca gcaaatcaat atcactgtgt ggcttaaggc cgccatccac tgcggagccg
      181 tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct
      241 gatagttgag tcgatacttc ggcgatcacc gcttccctca tactcttcct ttttcaatat
      301 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
      361 aaaaataaac aaatagctag ctcactcggt cgctacgctc cgggcgtgag actgcggcgg
      421 gcgctgcgga cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg
      481 tgaggcaaaa cagcagggcc gcgccggtgg cgtttttcca taggctccgc catcctgcca
      541 gagttcacat aaacagacgc ttttccggtg catctgtggg agccgtgagg ctcaaccatg
      601 aatctgacag tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggcgggt
      661 cgctccctct tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt
      721 tctcccttac gggaagtgtg gcgctttctc atagctaaca cactggtatc tcggctcggt
      781 gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg
      841 cgccttatcc ggtaactgtt cacttgagtc caacccggaa aagcacggta aaacgccact
      901 ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg
      961 ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc
     1021 tgcgaaagcc agttaccacg gttaagcagt tcaccaactg acttaacctt cgatcaaacc
     1081 acctcccaag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc
     1141 tcaagaagat cctttgatct tttctactga accgctctag atttcagtgc aatttatctc
     1201 ttcaaatgta gcacctgttt acggctagct cagtcctagg tatagtgcta gccgcttcta
     1261 gagtcacaca ggaaagtact agatgcagag ttcggtaaat gagttcctga ccccccgcca
     1321 catcgatgtg caggtggtca gtcaaacccg cgccaagatc acgctcgagc ctctcgagcg
     1381 tggttttggt cacaccctgg gcaacgcgct gcgtcgcatc ctgttgtcct ccatgcctgg
     1441 ctgcgcagtg gtcgaggccg agatcgacgg cgtactccac gagtactcgg cgatcgaagg
     1501 tgtgcaggaa gatgtaatcg agatcctgct gaacctgaaa ggtctggcca tcaagctgca
     1561 cggtcgtgat gaagtgacgc tgaccctggc taagaagggc tcgggtgttg tgactgctgc
     1621 cgatattcag ctggatcacg atgttgagat catcaacggt gaccacgtta tcgccaacct
     1681 ggcagacaac ggcgcgctga acatgaagct gaaggtagct cgtggccgtg gctacgagcc
     1741 tgccgacgca cgtcagagcg atgaagacga aagccgcagc atcggccgtc tgcagctcga
     1801 cgcatcgttc agcccggtcc gtcgtgtctc ctacgtggtg gaaaacgccc gtgtcgagca
     1861 gcgcaccaac ctggacaaac tggtcctgga cctggaaacc aacggcactc tggatcccga
     1921 agaggctatc cgtcgcgccg ctaccatcct gcaacagcag ctggcagcgt tcgtggacct
     1981 caagggaggt tctggaggtg gtgggtccat gaacgaaaaa gaagaactga aatctaaaaa
     2041 agcggaactg cgcaaccgca tcgaacagct gaaacagaaa cgcgaacagc tgaaacagaa
     2101 aatcgcgaac ctgcgcaaag aaatcgaagc gtataaataa tgaccgaaca aaaactcatc
     2161 tcagaagagg atctgaatag cgccgtcgac catcatcatc atcatcattg agtttaaacg
     2221 gtctccagct tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat
     2281 cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc
     2341 cacctgaccc aatgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt
     2401 ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa
     2461 gactgggcct ttcgttttat ctgttgtttg tcggtgaact ggatccttac tcgagtctag
     2521 actgcagctg aaacctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa
     2581 taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac
     2641 gaccgggtca tcgtggccgg atcttgcggc cactcggctt gaacgaattg ttagacatta
     2701 tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc
     2761 tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat
     2821 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg
     2881 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg
     2941 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcttt aaggtttcat ttagcgcctc
     3001 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc
     3061 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg
     3121 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt
     3181 agctggataa cgccacggaa tgatgtcg
//
