LOCUS       sequence-455587-       13208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mapping of cytosol-facing peroxisome outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13208)
  AUTHORS   Bao X, Jia H, Zhang X, Tian S, Zhao Y, Li X, Lin P, Ma C, Wang P, 
            Song CP, Zhu X
  TITLE     Mapping of cytosol-facing organelle outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
  JOURNAL   Plant J. 2024 Apr;118(1):7-23. doi: 10.1111/tpj.16641. Epub 2024 Jan
            23.
  PUBMED    38261530
REFERENCE   2  (bases 1 to 13208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16641";
            journalName: "Plant J"; date: "2024-04"; volume: "118"; issue: "1"; 
            pages: "7-23"
COMMENT     Sequence Label: PX2
FEATURES             Location/Qualifiers
     source          1..13208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..69)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(129..155)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(168..194)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(207..233)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(249..1208)
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             complement(join(1209..1919,1920..1922,1923..1925))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1209..1919,1920..1922,1923..1925))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1251..1272)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1600..1619
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(2246..2626)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     complement(3160..3177)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3160..3176)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3169..3191)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3379..3403
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4704..5333
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5762..6835
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6901..7095
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7331..7353)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7439..7579
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7494..7513
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7594..7611)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7765..8353)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7845..7864)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9639..10433)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10858..10882
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10960..11134
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11175..12200)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(12273..12618)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(12310..12330)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    12809..12830
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12845..12850,12851..12868,12869..12875)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12883..12899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12888..12910
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12907..12923
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12907..12923
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(12940..13192)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 ttacttatcg tcatcgtcct tgtaatcgat gtcgtgatcc ttatagtctc catcatggtc
       61 tttgtagtcc atggtaccgg atccactagt atttaaatgt cgaccccggg cccctgcaga
      121 agcttctaag cataatcagg cacatcataa gggtatccgg cgctgccagc gtagtcaggc
      181 acatcatagg ggtaggatcc tgtgccggcg taatcaggca cgtcataggg atacatgccg
      241 ctccctccct tttcggcaga ccgcagactg atttctccgc ccatccaggg tttgataact
      301 ccgtcctgtt ccagcagcag ggctccctgt ttatcaatcc cccggctaat cccgaaaatc
      361 tctttgtccc caatgatcag cttcacgggt ctattgatga agttatccag cttctcccac
      421 cgtggcagat atggagccag gccttcctgc tcgaacagtt ccagggctgc ccgcagctct
      481 cggatcagcg tagcggccag agtattcctg tccaggttaa tccctgcttc ctgcagtgtg
      541 atccagccct gattgaccac gctttcctcc acgcgcctca tagccacgtt aatcccggcg
      601 ccaatgacga tctgtgcagc atcgcctgtt attccggcca gctccaccag gatgcctgcc
      661 agctttctat cctgcagata caggtcattg ggccatttga ctcgcacctt gtctgctccc
      721 agctttcgca gcgcttctgc catgacaatt ccgatgaccg ggcccaggcc gattgctgct
      781 ggtccccgct tcaggcgcca aaacatactc aggtacaggt tagctccaaa aggagagaac
      841 catttcctcc ctctgcttcc tctccctgcc tgctgatatt ctgcaatgca agcatcccca
      901 ctcttcagct cgccgattcg atccagcagg tactgattgg tggagtcgac cacaggcagg
      961 actgccacgc tcccgccgtc cagctgtccc agaatctgtt tagcgttcag cagcgggata
     1021 ggctcgggca gagagtagcc ctttccgggc acggtgaaca cgtccacgcc ccagtcgcgc
     1081 agagtctgaa tgtgcttgtt gatagcggcc ctggacatgc ccagggtttc tcccagctgt
     1141 tcgccactat ggaactcgcc attagccagg agagcgatca gcttcagagg cacagtattg
     1201 tctttcatct tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
     1261 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
     1321 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
     1381 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
     1441 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
     1501 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
     1561 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
     1621 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
     1681 tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc
     1741 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
     1801 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
     1861 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
     1921 accatactag tctgagagag aagaatagca gtcagaacct gaagagactt caatcctcct
     1981 cctttcttct ttttcgtggt tatctttgca ttttgatcag ttgcagtcag cttttccaaa
     2041 gtctcatcat catcactctg accattgcaa agacgcgaag aatcaggcct ccgggaagcg
     2101 acccttgatt tcaggtaaac agcagttcca ccagcagcta caatcccagc cgcaagcagt
     2161 atagatttcc gtcttgacgc tacaagaccc cgaccccgct cagttaactg caatagttga
     2221 agtgaaggca taagctttct agagtcgatt tggtgtatcg agattggtta tgaaattcag
     2281 atgctagtgt aatgtattgg taatttggga agatataata ggaagcaagg ctatttatcc
     2341 atttctgaaa aggcgaaatg gcgtcaccgc gagcgtcacg cgcattccgt tcttgctgta
     2401 aagcgttgtt tggtacactt ttgactagcg aggcttggcg tgtcagcgta tctattcaaa
     2461 agtcgttaat ggctgcggat caagaaaaag ttggaataga aacagaatac ccgcgaaatt
     2521 caggcccggt tgccatgtcc tacacgccga aataaacgac caaattagta gaaaaataaa
     2581 aactgactcg gatacttacg tcacgtcttg cgcactgatt tgaaaaatct ccctcgatcg
     2641 agaaagagat caatgttgag ctgcttcaaa agcaatggga ttgaccagct cgcggatcct
     2701 acaggccaaa ttcgctctta gccgtacaat attactcacc ggtgcgatgc cccccatcgt
     2761 aggtgaaggt ggaaattaat gatccatctt gagaccacag gcccacaaca gctaccagtt
     2821 tcctcaaggg tccaccaaaa acgtaagcgc ttacgtacat ggtcgataag aaaaggcaat
     2881 ttgtagatgt taacatccaa cgtcgctttc agggatccta caggccaaat tcgctcttag
     2941 ccgtacaata ttactcaccg gtgcgatgcc ccccatcgta ggtgaaggtg gaaattaatg
     3001 atccatcttg agaccacagg cccacaacag ctaccagttt cctcaagggt ccaccaaaaa
     3061 cgtaagcgct tacgtacatg gtcgataaga aaaggcaatt tgtagatgtt aacatccaac
     3121 gtcgctttca gggatccgcg agcttatcga taccgtggca ctggccgtcg ttttacaacg
     3181 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     3241 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     3301 cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca
     3361 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     3421 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     3481 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg
     3541 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc
     3601 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
     3661 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
     3721 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
     3781 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
     3841 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
     3901 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
     3961 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
     4021 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
     4081 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     4141 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     4201 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     4261 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     4321 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     4381 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     4441 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     4501 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     4561 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     4621 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
     4681 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     4741 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
     4801 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     4861 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     4921 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     4981 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     5041 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     5101 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     5161 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     5221 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     5281 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     5341 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     5401 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     5461 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     5521 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
     5581 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     5641 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     5701 gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     5761 cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     5821 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     5881 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     5941 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     6001 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     6061 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     6121 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     6181 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     6241 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     6301 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     6361 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     6421 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     6481 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     6541 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     6601 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     6661 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     6721 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     6781 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
     6841 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc
     6901 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
     6961 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
     7021 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     7081 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     7141 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     7201 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     7261 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
     7321 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     7381 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     7441 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     7501 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     7561 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7621 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7681 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7741 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7801 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7861 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7921 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7981 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8041 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8101 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8161 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8221 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8281 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8341 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8401 aaactcacgt taaggaaggt gcgaacaagt ccctgatatg agatcatgtt tgtcatctgg
     8461 agccatagaa cagggttcat catgagtcat caacttacct tcgccgacag tgaattcagc
     8521 agtaagcgcc gtcagaccag aaaagagatt ttcttgtccc gcatggagca gattctgcca
     8581 tggcaaaaca tggtggaagt catcgagccg ttttacccca aggctggtaa tggccggcga
     8641 ccttatccgc tggaaaccat gctacgcatt cactgcatgc agcattggta caacctgagc
     8701 gatggcgcga tggaagatgc tctgtacgaa atcgcctcca tgcgtctgtt tgcccggtta
     8761 tccctggata gcgccttgcc ggaccgcacc accatcatga atttccgcca cctgctggag
     8821 cagcatcaac tggcccgcca attgttcaag accatcaatc gctggctggc cgaagcaggc
     8881 gtcatgatga ctcaaggcac cttggtcgat gccaccatca ttgaggcacc cagctcgacc
     8941 aagaacaaag agcagcaacg cgatccggag atgcatcaga ccaagaaagg caatcagtgg
     9001 cactttggca tgaaggccca cattggtgtc gatgccaaga gtggcctgac ccacagcctg
     9061 gtcaccaccg cggccaacga gcatgacctc aatcagctgg gtaatctgct gcatggagag
     9121 gagcaatttg tctcagccga tgccggctac caaggggcgc cacagcgcga ggagctggcc
     9181 gaggtggatg tggactggct gatcgccgag cgccccggca aggtaagaac cttgaaacag
     9241 catccacgca agaacaaaac ggccatcaac atcgaataca tgaaagccag catccgggcc
     9301 agggtggagc acccatttcg catcatcaag cgacagttcg gcttcgtgaa agccagatac
     9361 aaggggttgc tgaaaaacga taaccaactg gcgatgttat tcacgctggc caacctgttt
     9421 cgggcggacc aaatgatacg tcagtgggag agatctcact aaaaactggg gataacgcct
     9481 taaatggcga agaaacggtc taaataggct gattcaaggc atttacggga gaaaaaatcg
     9541 gctcaaacat gaagaaatga aatgactgag tcagccgaga agaatttccc cgcttattcg
     9601 caccttcctt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa
     9661 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
     9721 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
     9781 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
     9841 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
     9901 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
     9961 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
    10021 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
    10081 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
    10141 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
    10201 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
    10261 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
    10321 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
    10381 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
    10441 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
    10501 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
    10561 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
    10621 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
    10681 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
    10741 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
    10801 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
    10861 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
    10921 gtttttaatg tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga
    10981 ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata caaatacata
    11041 ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt
    11101 atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc gacagatccc
    11161 ggtcggcatc tactctattt ctttgccctc ggacgagtgc tggggcgtcg gtttccacta
    11221 tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt
    11281 gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa
    11341 gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg
    11401 agcatatacg cccggagtcg tggcgatcct gcaagctccg gatgcctccg ctcgaagtag
    11461 cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg
    11521 tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg
    11581 tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag
    11641 tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg
    11701 tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc
    11761 catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta
    11821 agatcggccg cagcgatcgc atccatagcc tccgcgaccg gttgtagaac agcgggcagt
    11881 tcggtttcag gcaggtcttg caacgtgaca ccctgtgaac ggcgggagat gcaataggtc
    11941 aggctctcgc taaactcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca
    12001 aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg
    12061 acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc
    12121 tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg
    12181 gtgagttcag gctttttcat atctcattgc cccccggatc tgcgaaagct cgagagagat
    12241 agatttgtag agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct
    12301 tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg
    12361 gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
    12421 atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg
    12481 atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc
    12541 cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc
    12601 ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg
    12661 gagtagacga gagtgtcgtg ctccaccatg ttggcaagct gctctagcca atacgcaaac
    12721 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    12781 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    12841 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    12901 ttcacacagg aaacagctat gacatgatta cgaattcccg atctagtaac atagatgaca
    12961 ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa
    13021 tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca
    13081 tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac
    13141 cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga tcggggaaat
    13201 tcgagctc
//
