LOCUS       sequence-45561-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Seeliger JC, Topp S, Sogi KM, Previti ML, Gallivan JP, Bertozzi CR
  TITLE     A riboswitch-based inducible gene expression system for 
            mycobacteria.
  JOURNAL   PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
  PUBMED    22279533
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(1)"; pages: "e29266. Epub 2012 Jan 18"
COMMENT     Sequence Label: pRiboS BsaHind
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(216..262)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cagttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg gctcataaca
       61 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
      121 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttcccc ccccccgacg
      181 tcaggtggct agctgatcac cgcggccatg atggcataaa acgaaaggcc cagtctttcg
      241 actgagcctt tcgttttatt tgatgcctgg cagtcgatcg tacgctagtt aactacgtcg
      301 acatcgataa gcttggtctc catcttgttg cctccttagc agggtgctgc caagggcatc
      361 aagacgatgc tggtatcacc ggtacctata gtgagtcgta tcccggtcgc gagtgccaac
      421 gttattctta gcactcgcct atgccgagtg caagaagccc cgcaccgggt catgcccctc
      481 gttcgacctt gtcctcggcc ctccgatccg ggtgagtatg ttcggcccat gaccgccaac
      541 gacaacaaga cccgtaaatg gtcggccgca gacgtccccg atcaaagcgg gcgcgttgtg
      601 gtcacctcta gatttaaaga tctggtaccg cggccgcacg cgttgcgctc ggtcgttcgg
      661 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      721 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaa
//
