LOCUS       sequence-45579-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J
  TITLE     Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and 
            cytotoxicity by amyloid-beta.
  JOURNAL   Nature. 2000 Jan 6. 403(6765):98-103.
  PUBMED    10638761
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2000 Jan 6. 403(6765):98-103."
COMMENT     Sequence Label: pcDNA-Caspase 12
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      80..89
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 cttggtaccg agctcggatc catggcggcc aggaggacac atgaaagaga tccaatctac
      121 aagatcaaag gtttggccaa ggacatgctg gatggggttt ttgatgacct ggtggagaag
      181 aatgttttaa atggagatga gttactcaaa ataggggaaa gtgcgagttt catcctgaac
      241 aaggctgaga atctggttga gaacttctta gagaaaacag acatggcagg aaaaatattt
      301 gctggccaca ttgccaattc ccaggaacag ctgagtttac aattttctaa tgatgaggat
      361 gatggacctc agaagatatg tacaccttct tctccatcag aatccaagag aaaagtagag
      421 gatgatgaaa tggaggtaaa tgctggattg gcccatgaat cacatctaat gctgacagct
      481 cctcatggac tccagagctc agaagtccaa gatacactga agctttgtcc acgtgatcag
      541 ttttgtaaga taaagacaga aagggcaaaa gagatatatc cagtgatgga gaaggaggga
      601 cgaacacgtc tggctctcat catctgcaac aaaaagtttg actacctttt tgatagagat
      661 aatgctgata ctgacatttt gaacatgcaa gaactacttg aaaatcttgg atactctgtg
      721 gtgttaaagg aaaaccttac agctcaggaa atggagacag agttaatgca gtttgctggc
      781 cgtccagagc accagtcctc agacagcaca ttcctggtgt ttatgtccca tggcatcctg
      841 gaaggaatct gtggggtga
//
