LOCUS       sequence-456018-        6868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AVPR1A receptor for split TEV assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Popovic L, Brankatschk B, Palladino G, Rossner MJ, Wehr MC
  TITLE     Polypharmacological profiling across protein target families and 
            cellular pathways using the multiplexed cell-based assay platform 
            safetyProfiler reveals efficacy, potency and side effects of drugs.
  JOURNAL   Biomed Pharmacother. 2024 Nov;180:117523. doi: 
            10.1016/j.biopha.2024.117523. Epub 2024 Oct 14.
  PUBMED    39405910
REFERENCE   2  (bases 1 to 6868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biomed 
            Pharmacother."; date: "2024-11"; volume: "180:117523. doi"; pages: "
            10.1016/j.biopha.2024.117523. Epub 2024 Oct 14"
COMMENT     Sequence Label: pTag4C_AVPR1A-V2R-NTEV-tcs-GV
FEATURES             Location/Qualifiers
     source          1..6868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    632..656
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      719..728
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1926..1950)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2441..2461
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2468..2497
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2507..2947
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2990..3010
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3161..3394
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     regulatory      3401..3410
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3413..3439
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3656..3777
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3693..3712)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3747..3766
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3784..4239)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3921..3942)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4133..4152
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4266..4370
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4372..4729
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4372..4563
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4395..4415)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4580..4715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4764..5558
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4764..5558
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4818..4837)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5428..5447
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5746..5765)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5790..5837
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6166..6754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6655..6674
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttatc aacaagtttg tacaaaaaag caggctctac
      661 catggacagc atgcgtctct ccgccggtcc cgacgcgggg ccctcgggca actccagccc
      721 atggtggcct ctggccaccg gcgctggcaa cacaagccgg gaggccgaag ccctcgggga
      781 gggcaacggc ccaccgaggg acgtgcgcaa cgaggagctg gccaaactgg agatcgccgt
      841 gctggcggtg actttcgcgg tggccgtgct gggcaacagc agcgtactgc tggctctgca
      901 ccggacgccg cgcaagacgt cccgcatgca cctcttcatc cgacacctca gcctggccga
      961 cctggccgtg gcattcttcc aggtgctgcc gcaaatgtgc tgggacatca cctaccgctt
     1021 ccgcggcccc gactggctgt gccgcgtggt gaagcacctg caggtgttcg gcatgtttgc
     1081 gtcggcctac atgctggtag tcatgacagc cgaccgctac atcgcggtgt gccacccgct
     1141 caagactctg caacagcccg cgcgccgctc gcgcctcatg atcgcggccg cctgggtgct
     1201 gagcttcgtg ctgagcacgc cgcagtactt cgtcttctcc atgatcgagg tgaacaatgt
     1261 caccaaggcc cgcgactgct gggccacctt catccagccc tggggttctc gtgcctacgt
     1321 gacctggatg acgggcggca tctttgtggc gcccgtggtc atcttgggta cctgctacgg
     1381 cttcatctgc tacaacatct ggtgcaacgt ccgcgggaag acggcgtcgc gccagagcaa
     1441 gggtgcagag caagcgggtg tggccttcca aaaggggttc ctgctcgcac cctgtgtcag
     1501 cagcgtgaag tccatttccc gggccaagat ccgcacggtg aagatgactt ttgtgatcgt
     1561 gacggcttac atcgtctgct gggcgccttt cttcatcatc cagatgtggt ctgtctggga
     1621 tcccatgtcc gtctggaccg aatcggaaaa ccctaccatc accatcactg cattactggg
     1681 ttccttgaat agctgctgta atccctggat atacatgttt tttagtggcc atctccttca
     1741 agactgtgtt caaagcttcc catgctgcca aaacatgaag gaaaaattca acaaagaaga
     1801 tactgacagt atgagcagaa gacagacttt ttattctaac aatcgaagcc caacaaacag
     1861 tacgggtatg tggaaggact cgcctaaatc ttccaagtcc atcaaattca ttcctgtttc
     1921 aactgaccca gctttcttgt acaaagtggt tgatggtacc gcccggggac gcaccccacc
     1981 cagcctgggt ccccaagatg agtcctgcac caccgccagc tcctccctgg ccaaggacac
     2041 ttcatcggcc agccctagca accctggcgc cagcaacggc agcggagaga gcttgtttaa
     2101 gggaccacgt gattacaacc cgatatcgag caccatttgt catttgacga atgaatctga
     2161 tgggcacaca acatcgttgt atggtattgg atttggtccc ttcatcatta caaacaagca
     2221 cttgtttcgt cgcaataatg gaacactgtt ggtccaatca ctacatggtg tattcaaggt
     2281 caaggacacc acgactttgc aacaacacct cattgatggg agggacatga tgattattcg
     2341 catgcctaag gatttcccac catttcctca aaagctgaaa tttagagagc cacaaaggga
     2401 agagcgcata tgtcttgtga caaccaactt ccaagctccc gagaacctgt acttccaggg
     2461 ttctagagaa cagaagctga tctcagaaga ggatctggcc cgggccatga agctactgtc
     2521 ttctatcgaa caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa
     2581 accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa
     2641 aaggtctccg ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga
     2701 acagctattt ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc
     2761 tttacaggat ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga
     2821 tgccgtcaca gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca
     2881 tagaataagt gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac
     2941 tgtatcgccg gaattcccgg ggatcagctt gataattcga ttcaccatgc ccaagaagaa
     3001 gaggaaggtg gcgtacagcc gcgcgcgtac gaaaaacaat tacgggtcta ccatcgaggg
     3061 cctgctcgat ctcccggacg acgacgcccc cgaagaggcg gggctggcgg ctccgcgcct
     3121 gtcctttctc cccgcgggac acacgcgcag actgtcgacg gcccccccga ccgatgtcag
     3181 cctgggggac gagctccact tagacggcga ggacgtcgcg atggcgcatg ccgacgcgct
     3241 agacgatttc gatctggaca tgttggggga cggggattcc ccgggtccgg gatttacccc
     3301 ccacgactcc gccccctacg gcgctctgga tatggccgac ttcgagtttg agcagatgtt
     3361 taccgatgcc cttggaattg acgagtacgg tggggcggcc gccaccatgg ggtatccata
     3421 tgatgttcca gattatgcta gatctggaat cactagtgaa ttatcgaatt ctgcagccaa
     3481 gctaattccg ggcgaatgtc ttatgattta tgatttttat tattaacctg cagggcggcc
     3541 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     3601 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     3661 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3721 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
     3781 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     3841 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     3901 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     3961 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     4021 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     4081 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     4141 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     4201 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     4261 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4321 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     4381 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4441 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4501 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4561 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4621 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4681 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     4741 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     4801 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4861 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4921 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     4981 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5041 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5101 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5161 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5221 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5281 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     5341 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5401 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5461 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5521 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     5581 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     5641 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     5701 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     5761 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     5821 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     5881 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     5941 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     6001 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6061 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6121 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6181 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6241 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6301 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6361 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6421 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6481 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6541 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6601 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6661 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6721 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6781 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6841 tgtggataac cgtattaccg ccatgcat
//
