LOCUS       sequence-456030-        3148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector pJET1.2 contains the 158-bp 5′-UTR fragment (from −158 to −1 
            bp before the ATG) of OsMac3 from Japonica rice genomic DNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Kiryushkin AS, Ilina EL, Kiikova TY, Pawlowski K, Demchenko KN
  TITLE     Do DEEPER ROOTING 1 Homologs Regulate the Lateral Root Slope Angle 
            in Cucumber (Cucumis sativus)?
  JOURNAL   Int J Mol Sci. 2024 Feb 6;25(4):1975. doi: 10.3390/ijms25041975.
  PUBMED    38396652
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2024-02-6"; volume: "25(4):1975. doi"; pages: " 
            10.3390/ijms25041975"
COMMENT     Sequence Label: pJET1.2-OsMac3
FEATURES             Location/Qualifiers
     source          1..3148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(609..615,616..633,634..639))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    654..675
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(795..812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(966..1554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1046..1065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1725..2516,2517..2585))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2348..2367
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2586..2690)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2758..2776
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     3082..3101
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3082..3100
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aatctagaat tgcgagacag tgccgtgggt aaatattgtg atctgttctc ttgagctgaa
       61 attcctgcct ttcttcttct acaggctaga gagtcccagg attcagaaaa tgaggagcaa
      121 atcttttgtt gtacttcttt ggtatgaaaa agctctcttt agtctttcta gattatcttt
      181 ctagaagatc tcctacaata ttctcagctg ccatggaaaa tcgatgttct tcttttattc
      241 tctcaagatt ttcaggctgt atattaaaac ttatattaag aactatgcta accacctcat
      301 caggaaccgt tgtaggtggc gtgggttttc ttggcaatcg actctcatga aaactacgag
      361 ctaaatattc aatatgttcc tcttgaccaa ctttattctg catttttttt gaacgaggtt
      421 tagagcaagc ttcaggaaac tgagacagga attttattaa aaatttaaat tttgaagaaa
      481 gttcagggtt aatagcatcc attttttgct ttgcaagttc ctcagcattc ttaacaaaag
      541 acgtctcttt tgacatgttt aaagtttaaa cctcctgtgt gaaattatta tccgctcata
      601 attccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      661 agctaactca cattaattgc gttgcgctca ctgccaattg ctttccagtc gggaaacctg
      721 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      781 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      841 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      901 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      961 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1021 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1081 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1141 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1201 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1261 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1321 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1381 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1441 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1501 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1561 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1621 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1681 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1741 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1801 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1861 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1921 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1981 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2041 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2101 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2161 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2221 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2281 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2341 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2401 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2461 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2521 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2581 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2641 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2701 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2761 aggcgtatca cgaggccgcc cctgcagccg aattatatta tttttgccaa ataattttta
     2821 acaaaagctc tgaagtcttc ttcatttaaa ttcttagatg atacttcatc tggaaaattg
     2881 tcccaattag tagcatcacg ctgtgagtaa gttctaaacc atttttttat tgttgtatta
     2941 tctctaatct tactactcga tgagttttcg gtattatctc tatttttaac ttggagcagg
     3001 ttccattcat tgtttttttc atcatagtga ataaaatcaa ctgctttaac acttgtgcct
     3061 gaacaccata tccatccggc gtaatacgac tcactatagg gagagcggcc gccagatctt
     3121 ccggatggct cgagtttttc agcaagat
//
