LOCUS       sequence-456147-        9420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-CDC25C
FEATURES             Location/Qualifiers
     source          1..9420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2179..2205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(3643..4217,4218..4220,4221..4229)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3810..3827)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4037..4056
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4230..5249
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    5277..5865
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5330..5350)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5748..5759)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5937..6170
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6248..6369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6285..6304)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6339..6358
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6409..6544
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6564..6583)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6565..6583)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6593..6610)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6593..6609)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6602..6624)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6751..7206
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6838..6857)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7048..7069
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7232..7336
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7337..7405,7406..8197)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7555..7574)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8368..8956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8857..8876
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9110..9127
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9244..9265
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9280..9285,9286..9303,9304..9310)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9318..9334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9323..9345
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9342..9358
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9342..9358
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9377..9397
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9379..9397
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgta cccctacgac
     2161 gtgcccgact acgccggcta tccgtatgat gtcccggact atgcaggaag cggatctacg
     2221 gaactcttct catccacaag agaggaagga agctctggct caggacccag ttttaggtct
     2281 aatcaaagga aaatgttaaa cctgctcctg gagagagaca cttcctttac cgtctgtcca
     2341 gatgtcccta gaactccagt gggcaaattt cttggtgatt ctgcaaacct aagcattttg
     2401 tctggaggaa ccccaaaacg ttgcctcgat ctttcgaatc ttagcagtgg ggagataact
     2461 gccactcagc ttaccacttc tgcagacctt gatgaaactg gtcacctgga ttcttcagga
     2521 cttcaggaag tgcatttagc tgggatgaat catgaccagc acctaatgaa atgtagccca
     2581 gcacagcttc tttgtagcac tccgaatggt ttggaccgtg gccatagaaa gagagatgca
     2641 atgtgtagtt catctgcaaa taaagaaaat gacaatggaa acttggtgga cagtgaaatg
     2701 aaatatttgg gcagtcccat tactactgtt ccaaaattgg ataaaaatcc aaacctagga
     2761 gaagaccagg cagaagagat ttcagatgaa ttaatggagt tttccctgaa agatcaagaa
     2821 gcaaaggtga gcagaagtgg cctatatcgc tccccgtcga tgccagagaa cttgaacagg
     2881 ccaagactga agcaggtgga aaaattcaag gacaacacaa taccagataa agttaaaaaa
     2941 aagtattttt ctggccaagg aaagctcagg aagggcttat gtttaaagaa gacagtctct
     3001 ctgtgtgaca ttactatcac tcagatgctg gaggaagatt ctaaccaggg gcacctgatt
     3061 ggtgattttt ccaaggtatg tgcgctgcca accgtgtcag ggaaacacca agatctgaag
     3121 tatgtcaacc cagaaacagt ggctgcctta ctgtcgggga agttccaggg tctgattgag
     3181 aagttttatg tcattgattg tcgctatcca tatgagtatc tgggaggaca catccaggga
     3241 gccttaaact tatatagtca ggaagaactg tttaacttct ttctgaagaa gcccatcgtc
     3301 cctttggaca cccagaagag aataatcatc gtgttccact gtgaattctc ctcagagagg
     3361 ggcccccgaa tgtgccgctg tctgcgtgaa gaggacaggt ctctgaacca gtatcctgca
     3421 ttgtactacc cagagctata tatccttaaa ggcggctaca gagacttctt tccagaatat
     3481 atggaactgt gtgaaccaca gagctactgc cctatgcatc atcaggacca caagactgag
     3541 ttgctgaggt gtcgaagcca gagcaaagtg caggaagggg agcggcagct gcgggagcag
     3601 attgcccttc tggtgaagga catgagccca tgagcaattc cgcccctctc cctccccccc
     3661 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     3721 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     3781 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     3841 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     3901 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     3961 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     4021 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     4081 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     4141 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     4201 aaaaacacga tgataatatg gccacaacca tgaaaaagcc tgaactcacc gctacctctg
     4261 tcgagaagtt tctgatcgaa aagttcgaca gtgttagcga cctgatgcag ctctccgagg
     4321 gcgaagaatc tcgggctttc agcttcgatg tgggagggcg tggatatgtc ctgcgggtga
     4381 atagctgcgc cgatggtttc tacaaagatc gctatgttta tcggcacttt gcatccgccg
     4441 ctctccctat tcccgaagtg cttgacattg gggagttcag cgagagcctg acctattgca
     4501 tctcccgccg tgcacagggt gtcaccttgc aagacctgcc tgaaaccgaa ctgcccgctg
     4561 ttctccagcc cgtcgccgag gccatggatg ccatcgctgc cgccgatctt agccagacca
     4621 gcgggttcgg cccattcgga cctcaaggaa tcggtcaata cactacatgg cgcgatttca
     4681 tctgcgctat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     4741 gtgcctccgt cgcccaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     4801 tccggcacct cgtgcacgcc gatttcggct ccaacaatgt cctgaccgac aatggccgca
     4861 taacagccgt cattgactgg agcgaggcca tgttcgggga ttcccaatac gaggtcgcca
     4921 acatcttctt ctggaggccc tggttggctt gtatggagca gcagacccgc tacttcgagc
     4981 ggaggcatcc cgagcttgca ggatctcctc ggctccgggc ttatatgctc cgcattggtc
     5041 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggctcagg
     5101 gtcgctgcga cgcaatcgtc cggtccggag ccgggactgt cgggcgtaca caaatcgccc
     5161 gcagaagcgc tgccgtctgg accgatggct gtgtggaagt gctcgccgat agtggaaaca
     5221 gacgccccag cactcgtcct agggcaaagg atctgcagta gacgcgttaa gtcgacaatc
     5281 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5341 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5401 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5461 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5521 ggggcattgc caccacctgt caactccttt ccgggacttt cgctttcccc ctccctattg
     5581 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5641 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     5701 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5761 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5821 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     5881 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5941 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     6001 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6061 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6121 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     6181 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6241 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6301 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6361 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     6421 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6481 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6541 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     6601 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6661 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6721 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     6781 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6841 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6901 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6961 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7021 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7081 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7141 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     7201 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7261 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7321 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7381 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7441 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7501 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7561 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7621 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7681 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7741 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7801 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7861 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7921 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7981 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8041 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8101 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8161 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8221 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8281 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8341 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8401 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8461 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8521 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8581 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8641 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8701 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8761 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8821 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8881 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8941 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9001 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9061 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9121 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     9181 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     9241 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     9301 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     9361 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
//
