LOCUS       sequence-45618-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Banko MR, Allen JJ, Schaffer BE, Wilker EW, Tsou P, White JL, Villen
            J, Wang B, Kim SR, Sakamoto K, Gygi SP, Cantley LC, Yaffe MB, Shokat
            KM, Brunet A
  TITLE     Chemical Genetic Screen for AMPKalpha2 Substrates Uncovers a Network
            of Proteins Involved in Mitosis.
  JOURNAL   Mol Cell. 2011 Dec 23;44(6):878-92. Epub 2011 Dec 1.
  PUBMED    22137581
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-12-23"; volume: "44(6)"; pages: "878-92. Epub 2011 Dec 
            1"
COMMENT     Sequence Label: pECE-M2-AMPKalpha2 M93G
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..79
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agaggctttt ttggaggaga tctaagcttg gtaccgagct cggatcctca ggatggacta
       61 caaggacgat gacgataaac ccgggaattc ggctgagaag cagaagcacg acgggcgggt
      121 gaagatcgga cactacgtgc tgggcgacac gctgggcgtc ggcaccttcg gcaaagtgaa
      181 gattggagaa catcaattaa caggccataa agtggcagtt aaaatcttaa atagacagaa
      241 gattcgcagt ttagatgttg ttggaaaaat aaaacgagaa attcaaaatc taaaactctt
      301 tcgtcatcct catattatca aactatacca ggtgatcagc actccaacag atttttttat
      361 ggtaggggaa tatgtgtctg gaggtgaatt atttgactac atctgtaagc atggacgggt
      421 tgaagagatg gaagccaggc ggctctttca gcagattctg tctgctgtgg attactgtca
      481 taggcatatg gttgttcatc gagacctgaa accagagaat gtcctgttgg atgcacacat
      541 gaatgccaag atagccgatt tcggattatc taatatgatg tcagatggtg aatttctgag
      601 aactagttgc ggatctccaa attatgcagc acctgaagtc atctcaggca gattgtatgc
      661 aggtcctgaa gttgatatct ggagctgtgg tgttatcttg tatgctcttc tttgtggcac
      721 cctcccattt gatgatgagc atgtacctac gttatttaag aagatccgag ggggtgtctt
      781 ttatatccca gaatatctca atcgttctgt cgccactctc ctgatgcata tgctgcaggt
      841 tgacccactg aaacgagcaa ctatcaaaga cataa
//
