LOCUS       sequence-456195-       12725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-term transit peptide from Sapria himalayana Asparagine 
            aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 419.110 
            (SHI02794); determination of S. himalayana AsnRS target 
            localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12725)
  AUTHORS   DeTar RA, Chustecki JM, Martinez-Hottovy A, Ceriotti LF, Broz AK, 
            Lou X, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB
  TITLE     Photosynthetic demands on translational machinery drive retention of
            redundant tRNA metabolism in plant organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2421485121. doi: 
            10.1073/pnas.2421485121. Epub 2024 Dec 18.
  PUBMED    39693336
REFERENCE   2  (bases 1 to 12725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-12-24"; volume: "121(52):e2421485121. 
            doi"; pages: " 10.1073/pnas.2421485121. Epub 2024 Dec 18"
COMMENT     Sequence Label: Shim_AsnRS_419.110_SHI02794_TP_GFP_pK7fWG2_IVD...
FEATURES             Location/Qualifiers
     source          1..12725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4981..5005
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        5675..6019
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    6031..6055
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6947..6971)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6973..6999
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        7902..8246
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    8346..8370
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8635..8659)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(8667..8669,8670..8672,8673..9386)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8667..8669,8670..8672,8673..9383)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8712..8733)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8973..8992)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9320..9341
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        9752..9931
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             9965..10759
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10019..10038)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10629..10648
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      10799..11051
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    11144..11168
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11689..12480
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttaa agtactttaa agtactttaa
     4861 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
     4921 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
     4981 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
     5041 aatctgatcc aagctcaagc taagcttgag ctctcccata tggtcgacta gagccaagct
     5101 gatctccttt gccccggaga tcaccatgga cgactttctc tatctctacg atctaggaag
     5161 aaagttcgac ggagaaggtg acgataccat gttcaccacc gataatgaga agattagcct
     5221 cttcaatttc agaaagaatg ctgacccaca gatggttaga gaggctgcag gtcaacatgg
     5281 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
     5341 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
     5401 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
     5461 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
     5521 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     5581 agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata
     5641 tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat
     5701 ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg
     5761 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag
     5821 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     5881 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
     5941 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     6001 catttcattt ggagaggacc tcgagaattc acaagtttgt acaaaaaagc aggctccgcg
     6061 gccgccccct tcaccatgca gaggtttttc tccgccagat cgattctcgg ttacgccgtc
     6121 aagacgcgga ggaggtcttt ctcttctcgt tcttcgtctc tcctattcga cgacactcag
     6181 ttacagttta aagaaagtgt atccaagttt gcgcaagata ggatcccgca cgggatgaac
     6241 tcactgatca aggaaaatat gcgtatgatg gtggtcatgg aaggttcggt caacggctac
     6301 caattcaaat gcacaggtga aggagatggc aatccataca tgggaactca aaccatgagg
     6361 atcaaagtcg tcgagggagg acctctgcca tttgcctttg acattcttgc cacaagcttc
     6421 atgtatggca gcaagacttt tatcaagcac actaaaggca ttcctgattt ctttaaacag
     6481 tcctttcctg agggttttac ttgggaaaga gttacaagat acgaagatgg tggagtcttt
     6541 accgttatgc aggacaccag ccttgaagat ggctgtctcg tttaccacgc caaagtcact
     6601 ggggtaaact ttccctccaa tggtgccgtg atgcagaaga agaccaaggg ttgggagcca
     6661 aatacagaga tgctgtatcc agcagatggt ggtctgaggg gatactctca aatggcactg
     6721 aatgttgatg gtggtggcta tctgtcttgc tctttcgaaa caacttacag gtcaaaaaag
     6781 accgtcgaga acttcaagat gcccggtttc cattttgttg atcaccgcct ggaaaggtta
     6841 gaggaaagtg acaaggaaat gttcgtagta caacacgaac acgcagttgc caagttctgt
     6901 gaccttccat ccaaactggg acgtctttga aagggtgggc gcgccgaccc agctttcttg
     6961 tacaaagtgg tttacccata cgatgttcca gattacgctt gagagctcgc ataattctca
     7021 acacaacata tacaaaacaa acgaatctca agcaatcaag cattctactt ctattgcagc
     7081 aatttaaatc atttctttta aagcaaaagc aattttctga aaattttcac catttacgaa
     7141 cgatagccat ggcactcatc tttggcacag tcaacgctaa catcctgaag gaagtgttcg
     7201 gtggagctcg gtacccgggg atcctctaga gtccgcaaaa atcaccagtc tctctctaca
     7261 aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat
     7321 tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
     7381 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga
     7441 cctgcaggca tgcaagcttg gcactggccc tacgcggcag gtctcatcaa gacgatctac
     7501 ccgagtaata atctccagga gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa
     7561 agattcagga ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt
     7621 actattccag tatggacgat tcaaggcttg cttcataaac caaggcaagt aatagagatt
     7681 ggagtctcta agaaagtagt tcctactgaa tcaaaggcca tggagtcaaa aattcagatc
     7741 gaggatctaa cagaactcgc cgtgaagact ggcgaacagt tcatacagag tcttttacga
     7801 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacactct cgtctactcc
     7861 aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
     7921 gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg
     7981 acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc
     8041 gttcaagatg cccctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     8101 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
     8161 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     8221 ggaagttcat ttcatttgga gaggactccg gtatttttac aacaattacc acaacaaaac
     8281 aaacaacaaa caacattaca atttactatt ctagtcgacc tgcaggcggc cgcactagtg
     8341 atatcacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atgcttgatt
     8401 ctaaggttct tcttgctctt tatcatcatc ttggacaact tattcatcat ggaatggtta
     8461 gagctgctat tggaaagttt aatgctcttg gacttattac ttctagatat tttccatctc
     8521 catctggaag aagacaacca gctctttctt ctcattgtca tcttccaatt tctcttgctc
     8581 caccaagaat gggagatcat aagactggac tttctcaaaa gggtgggcgc gccgacccag
     8641 ctttcttgta caaagtggtg atatcaatgg tgagcaaggg cgaggagctg ttcaccgggg
     8701 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     8761 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     8821 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     8881 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     8941 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     9001 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     9061 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     9121 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     9181 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     9241 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     9301 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     9361 tcggcatgga cgagctgtac aagtaaccgc ggccatgcta gagtccgcaa aaatcaccag
     9421 tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc
     9481 agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc
     9541 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     9601 aaaatccagt gacctgcagg catgcgacgt cgggccctct agaggatccc cgggtaccgc
     9661 gaattatcga tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc
     9721 gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactgagc
     9781 cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc
     9841 aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga
     9901 cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc aactcgatcg
     9961 aggcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
    10021 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
    10081 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
    10141 actccaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
    10201 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
    10261 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
    10321 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
    10381 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
    10441 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcggatgcc
    10501 cgacggcgag gatctcgtcg tgacccacgg cgatgcctgc ttgccgaata tcatggtgga
    10561 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
    10621 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
    10681 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
    10741 tcttgacgag ttcttctgag cgggactctg gggttcggac tctagctaga gtcaagcaga
    10801 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
    10861 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
    10921 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
    10981 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
    11041 gttactagat cgaccggcat gcaagctgat aattcaattc ggcgttaatt cagtacatta
    11101 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
    11161 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
    11221 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
    11281 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
    11341 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
    11401 tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
    11461 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
    11521 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
    11581 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
    11641 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg
    11701 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
    11761 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
    11821 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
    11881 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
    11941 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
    12001 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
    12061 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
    12121 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
    12181 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
    12241 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
    12301 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
    12361 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
    12421 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
    12481 tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
    12541 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
    12601 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa
    12661 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    12721 tcttc
//
