LOCUS       sequence-456204-        9855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-MAP4K1
FEATURES             Location/Qualifiers
     source          1..9855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2179..2205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(4687..5261,5262..5264,5265..5273)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4854..4871)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5081..5100
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    5712..6300
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5765..5785)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6183..6194)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6372..6605
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6683..6804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6720..6739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6774..6793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6844..6979
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6999..7018)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7000..7018)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7028..7045)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7028..7044)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7037..7059)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7186..7641
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7273..7292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7667..7771
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7772..7840,7841..8632)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7990..8009)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8803..9391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9292..9311
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9545..9562
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9679..9700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9715..9720,9721..9738,9739..9745)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9753..9769
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9758..9780
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9777..9793
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9777..9793
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9812..9832
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9814..9832
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgta cccttacgac
     2161 gttcctgatt acgcgggata tccctacgac gtgccagatt acgcgggtag tggtgacgtc
     2221 gtggaccctg acattttcaa tagagacccc cgggaccact atgacctgct acagcggctg
     2281 ggtggcggca cgtatgggga agtctttaag gctcgagaca aggtgtcagg ggacctggtg
     2341 gcactgaaga tggtgaagat ggagcctgat gatgatgtct ccacccttca gaaggaaatc
     2401 ctcatattga aaacttgccg gcacgccaac atcgtggcct accatgggag ttatctctgg
     2461 ttgcagaaac tctggatctg catggaattc tgtggggctg gttctctcca ggacatctac
     2521 caagtgacag gctccctgtc agagctccag attagctatg tctgccggga agtgctccag
     2581 ggactggcct atttgcactc acagaagaag atacacaggg acatcaaggg agctaacatc
     2641 ctcatcaatg atgctgggga ggtcagattg gctgactttg gcatctcggc ccagattggg
     2701 gctacactgg ccagacgcct ctctttcatt gggacaccct actggatggc tccggaagtg
     2761 gcagctgtgg ccctgaaggg aggatacaat gagctgtgtg acatctggtc cctgggcatc
     2821 acggccatcg aactggccga gctacagcca ccgctctttg atgtgcaccc tctcagagtt
     2881 ctcttcctca tgaccaagag tggctaccag cctccccgac tgaaggaaaa aggcaaatgg
     2941 tcggctgcct tccacaactt catcaaagtc actctgacta agagtcccaa gaaacgaccc
     3001 agcgccacca agatgctcag tcatcaactg gtatcccagc ctgggctgaa tcgaggcctg
     3061 atcctggatc ttcttgacaa actgaagaat cccgggaaag gaccctccat tggggacatt
     3121 gaggatgagg agcccgagct accccctgct atccctcggc ggatcagatc cacccaccgc
     3181 tccagctctc tggggatccc agatgcagac tgctgtcggc ggcacatgga gttcaggaag
     3241 ctccgaggaa tggagaccag acccccagcc aacaccgctc gcctacagcc tcctcgagac
     3301 ctcaggagca gcagccccag gaagcaactg tcagagtcgt ctgacgatga ctatgacgac
     3361 gtggacatcc ccacccctgc agaggacaca cctcctccac ttccccccaa gcccaagttc
     3421 cgttctccat cagacgaggg tcctgggagc atgggggatg atgggcagct gagcccgggg
     3481 gtgctggtcc ggtgtgccag tgggccccca ccaaacagcc cccgtcctgg gcctccccca
     3541 tccaccagca gcccccacct caccgcccat tcagaaccct cactctggaa cccaccctcc
     3601 cgggagcttg acaagccccc acttctgccc cccaagaagg aaaagatgaa gagaaaggga
     3661 tgtgcccttc tcgtaaagtt gttcaatggc tgccccctcc ggatccacag cacggccgcc
     3721 tggacacatc cctccaccaa ggaccagcac ctgctcctgg gggcagagga aggcatcttc
     3781 atcctgaacc ggaatgacca ggaggccacg ctggaaatgc tctttcctag ccggactacg
     3841 tgggtgtact ccatcaacaa cgttctcatg tctctctcag gaaagacccc ccacctgtat
     3901 tctcatagca tccttggcct gctggaacgg aaagagacca gagcaggaaa ccccatcgct
     3961 cacattagcc cccaccgcct actggcaagg aagaacatgg tttccaccaa gatccaggac
     4021 accaaaggct gccgggcgtg ctgtgtggcg gagggtgcga gctctggggg cccgttcctg
     4081 tgcggtgcat tggagacgtc cgttgtcctg cttcagtggt accagcccat gaacaaattc
     4141 ctgcttgtcc ggcaggtgct gttcccactg ccgacgcctc tgtccgtgtt cgcgctgctg
     4201 accgggccag gctctgagct gcccgctgtg tgcatcggcg tgagccccgg gcggccgggg
     4261 aagtcggtgc tcttccacac ggtgcgcttt ggcgcgctct cttgctggct gggcgagatg
     4321 agcaccgagc acaggggacc cgtgcaggtg acccaggtag aggaagatat ggtgatggtg
     4381 ttgatggatg gctctgtgaa gctggtgacc ccggaggggt ccccagtccg gggacttcgc
     4441 acacctgaga tccccatgac cgaagcggtg gaggccgtgg ctatggttgg aggtcagctt
     4501 caggccttct ggaagcatgg agtgcaggtg tgggctctag gctcggatca gctgctacag
     4561 gagctgagag accctaccct cactttccgt ctgcttggct cccccaggcc tgtagtggtg
     4621 gagacacgcc cagtggatga tcctactgct cccagcaacc tctacatcca ggaatgagca
     4681 attccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     4741 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     4801 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     4861 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     4921 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     4981 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     5041 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     5101 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     5161 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     5221 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatgaaga
     5281 ccttcaacat ctctcagcag gaccttgagc tggtggaagt tgccactgag aaaatcacca
     5341 tgctctatga ggacaacaag caccatgttg gtgctgccat caggaccaag acaggagaaa
     5401 tcatctctgc tgtccacatt gaagcctaca ttggcagggt cactgtgtgt gcagaggcca
     5461 ttgccattgg gtctgcagtc tccaatgggc agaaggactt tgacaccatt gtggctgtga
     5521 ggcaccccta cagtgatgag gtggacaggt ccatcagagt ggtgtccccc tgtggcatgt
     5581 gcagggagct catctcagac tatgcccctg attgctttgt tctgattgag atgaatggca
     5641 agctggtcaa gacaaccatt gaggagctga ttccactgaa atacaccaga aactaaacgc
     5701 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5761 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5821 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5881 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5941 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6001 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6061 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6121 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6181 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6241 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6301 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6361 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     6421 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6481 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6541 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6601 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     6661 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     6721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6781 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     6841 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6901 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     6961 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     7021 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     7081 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     7141 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     7201 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7261 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7321 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7381 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7441 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtagactc ttgttccaaa
     7501 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7561 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7621 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7681 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7741 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7801 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7861 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     7921 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     7981 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8041 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8101 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8161 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8221 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8281 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8341 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8401 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8461 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8521 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8581 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8641 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8701 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8761 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8821 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8881 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8941 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9001 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9061 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9121 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9181 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9241 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9301 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9361 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9421 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9481 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9541 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9601 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     9661 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     9721 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     9781 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     9841 gctggagctg caagc
//
