LOCUS       sequence-45623-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Banko MR, Allen JJ, Schaffer BE, Wilker EW, Tsou P, White JL, Villen
            J, Wang B, Kim SR, Sakamoto K, Gygi SP, Cantley LC, Yaffe MB, Shokat
            KM, Brunet A
  TITLE     Chemical Genetic Screen for AMPKalpha2 Substrates Uncovers a Network
            of Proteins Involved in Mitosis.
  JOURNAL   Mol Cell. 2011 Dec 23;44(6):878-92. Epub 2011 Dec 1.
  PUBMED    22137581
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-12-23"; volume: "44(6)"; pages: "878-92. Epub 2011 Dec 
            1"
COMMENT     Sequence Label: pECE-M2-PPP1R12C wt
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..79
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agaggctttt ttggaggaga tctaagcttg gtaccgagct cggatcctca ggatggacta
       61 caaggacgat gacgataaac ccgggaattc gtctggtgag gatggaccag ctgcaggccc
      121 cggcgctgcc gccgcagccg ccagggagcg ccgcagagag caactgcgtc agtggggggc
      181 ccgtgccgga gctgagcccg gacccggaga gcgcagagcc cgcactgtgc gcttcgagcg
      241 cgccgctgag ttccttgccg cttgcgctgg cggggacctc gacgaggctc gtctgatgct
      301 gcgcgctgct gatcctgggc cgggcgccga actggacccc gccgcaccac caccagctag
      361 ggctgtgctg gatagtacta acgctgatgg aatcagcgcc ctgcaccagg cctgtatcga
      421 tgagaatctg gaggtcgtgc gctttctggt cgagcagggc gcaactgtga atcaggccga
      481 taacgaaggc tggactccgc tgcacgtcgc tgcctcttgc ggttacctgg acatagcccg
      541 ctacctgctg tctcacgggg ctaacattgc cgctgtgaac agtgacggag acctgccact
      601 cgatctggca gagtccgacg caatggaagg actgctgaaa gccgagatcg cccgtcgggg
      661 tgtggacgtc gaggccgcca agagggccga ggaggagctg ctgctgcatg atacacgctg
      721 ttggctgaat ggcggcgcca tgcccgaggc acgccatcct cgcaccggcg ccagtgcact
      781 gcatgtggcc gctgccaagg gatacataga ggtgatgcgc ctgctgctgc aggccgggta
      841 cgaccca
//
