LOCUS       sequence-456263-       15420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The STU backbone vector for rice genome editing is based on 
            Mb3Cas12a-R (D172R).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15420)
  AUTHORS   Liu S, He Y, Fan T, Zhu M, Qi C, Ma Y, Yang M, Yang L, Tang X, Zhou 
            J, Zhong Z, An X, Qi Y, Zhang Y
  TITLE     PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single 
            transcript unit systems for efficient singular and multiplexed 
            genome editing in rice, maize, and tomato.
  JOURNAL   Plant Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486.
  PUBMED    39387219
REFERENCE   2  (bases 1 to 15420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486."
COMMENT     Sequence Label: pGEL1026
FEATURES             Location/Qualifiers
     source          1..15420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    199..223
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(244..492)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     ncRNA           complement(507..574)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             901..1206
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1085..1104
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     ncRNA           complement(1234..1270)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             complement(1367..1414)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(5222..5242)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5245..5254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        7331..8007
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     7944..7964
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    9097..9271
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    9349..9373
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9798..10592
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10679..11267
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11168..11187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11421..11438
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11453..11593
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11519..11538)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11669..11691
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11927..12121
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12187..13260)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13689..14318)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca
       61 gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
      121 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat tattctaata
      181 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttgag
      241 ctccttatct ttaatcatat tccatagtcc ataccatagc acatacagta gttatatgct
      301 gcagaagaga tccaacaaaa cattcacaat ggattataga aacatttgtt tattcattat
      361 aatgtgatct tacattcatt taatattaga aaaagccaca aattcataac acaacaagcc
      421 aagaaaaaaa cacaaactta agcacacaag ctttttattt gacacaccaa atatttcatc
      481 ttcatcttca tattctagac tcgagtgtcc cattcgccat gccgaagcat gttgcccagc
      541 cggcgccagc gaggaggctg ggaccatgcc ggcctgagac caagccagat aacagtatgc
      601 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat
      661 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      721 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga
      781 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      841 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa
      901 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      961 cagagtgata ttattgacac tcccgggcga cggatggtga tccccctggc cagtgcacgt
     1021 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
     1081 tggcgcatga tgaccaccga tatggccagt gtgccggttt ccgttatcgg ggaagaagtg
     1141 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
     1201 atataaggtc tccatctacc atagtagaaa ttagacgagc ttactcgttt cgtcctcacg
     1261 gactcatcag taattttttt tttttttttt tttttttttt tttttttttt tttttttttt
     1321 tttttttttt tttttttttt tttttttttt tttttttttt ttttcacttc tttttcttag
     1381 cctgtccggc ctttttggtg gcagcaggac gcttgcggtt ctgcgcgaaa ttcagccatg
     1441 tctgattgtc aatggcgagc ttcaccttgt tcagatcgtc tgagttcttg agctcattca
     1501 ggagccacag gcccttgagg gcaatgtggt atgcgccgtt ggcatccgca ttctgtggct
     1561 gtgtatcatc ggccagtgct gaattgaaga agacgccctc atcgttggcc acaggagaga
     1621 ggatgaaatc ctcgtcgctg gaggcgtttg agtacctgag ggccaggagt gtcttcagga
     1681 ggtacatcag agacttatgg aactccttgt cattgttctg gcagatatcc atgacgagat
     1741 ttggctgctt gtcgttaatg tggtaccggg tgaacagtga cttgagctca tcattgacgt
     1801 tcacgccgat tgtcgcgccc ttattctggt tggcggtctt gtcgtacacg tagcgcttat
     1861 cgccatggct gcaaatcttc cagatctgcc tggaattctt cgccttgtcg ttgaacttgg
     1921 cgtaatcaat gtggaactcg aagtagcccc ggtccgcatt gtagcagatc ttatcgaact
     1981 tgccgaagaa cgcctgagac tgggcaatgt tctcgtagcg aggcttcagg aggtccacga
     2041 agcctgtctc cggatcaatc tttgaggtgt tccaggcagg cacgtagaag aggaagccgg
     2101 tctgcttgcc gatagacttc aggtctgtga aattgttggt cagctggagc gcattcttgt
     2161 atgagccgat ctcatcgtcg gccttatcct tcaggacgag gtggttgagc ttcttaatca
     2221 gcgcattctc gaagttctgg tagatctgct tctccacctt gaacctgccc cgcttgaagc
     2281 cgaaattcag gtcctcgagg accacaatgg cgttgtactt cagcatgagc tggctgatct
     2341 gatggaccac gtgagagagg tagcctgact tcagctcctt gattgtctcg atctcgcccc
     2401 atcccacccg ggcattgagc ctctcaatct ccctcttgtc caggatctta tggtatggtg
     2461 tggtcatctg tgtgccattc gcagaggctg tggtgatatc gttgagtgac ctctgctcca
     2521 gaatctcgcc cttagaattg atgacggtga ggtacaggag gtgcctctcg ccgcgatcaa
     2581 ttccaatgac gttcacctca tcgtactgct ggattgactg gttcaccttc ttattgaact
     2641 ccttaattgt catgccctgg acgccgaaat tcatggtgat cggcacatgg agcatgaact
     2701 tatcctgtgt gtaccgctta tccttaatga tgtcgtagac gaactggcgc ttcttcgggt
     2761 tgtcaggatt cttgttctcg agcacctcgc cggccctgtg aattgttgtc tcgttcatat
     2821 ccagggacgc cttccggtag aaaatctcgg cctcgccgtt gagcttgtag ataggattca
     2881 cgaggttgtc ctcgctgaac agcgccttga agtacagggt atggagatta ggcttgccgt
     2941 gggcctttgg ggagaaatcc ttgttgtaga tctggaacag gtagagctgg ccctgctcga
     3001 cgagctcatt aatgtaatcg gcgttgatgt ccacgaactt cacctggtag ccctgtggct
     3061 ccacctcgcg gtagaaatcg ctgaggtcct ggtaagatga tgttggggag aacttgaagc
     3121 cgaaatgctg ccactccggg tgcttattaa tgcccgcctt gaagaagtcg atgagggcat
     3181 ggcaatcctt cagattgaag ttgtcgccct tcttgtgggt gccctgcgcg tacttgtcca
     3241 ggagctcggc gctaggattg tagtaatcga ggttggactt cgcgaagaac acctttggca
     3301 gcatcttgtt tgggcccggc aggagcttgt agatcatctt ctggtagacg ctcttgcctg
     3361 tattcggggc gttgtcgaac accttcttgt gcgccttatc caggagggcg aggtagtagc
     3421 agccgtcctt ctgcaggatg acgccgaaat tatccttctc cttgttcagg tcccagccat
     3481 tcaggagtgt cggattgccg aagttgagct tgtacttctc ggtagagaaa ggcttctgtg
     3541 agaggtaatc gcgcaccttg ttgtacaggg tcgcgatctt ggcgagctcg tcgtacagcg
     3601 cgccgaactc gccgtagaag ttgccatcct gattatggag tgtggtcttt gtggtcagga
     3661 gcttcgcgaa gtgggcgaca ttgagggcgt tgtccaggag ttccttcagc tgccgaatct
     3721 ctggagactt atcggacttg atctttggca gggccctctc gccggccggg cgctcccgct
     3781 cgaggaagcc cttgattgtg ctatgattgt tgtgaatctt ctggattggg ttgtccacgc
     3841 cggcgaggcc atgcttgaag tactgtccca gcttgccggc ctgcactgac tcatcgtcat
     3901 gcctggcggt gtagtgctca atggcctgct ccaggctggc cagtgagtgc actcccttaa
     3961 tgaacttgtc cttctccttt gtgagcttcg ccttggcgtt atcggtcttc gccttggcga
     4021 agcgctcgtt gaactccgga ttgaccacat cgacgtagta tccatccagc accctgccca
     4081 ggagggcgaa atcgccgaag gcctgcttgc tcagctcatt gaggttcttg tactcgacgt
     4141 agatgccgtc cttctggtaa tcgtcgaagc catcgaagag ggactggacc ttcgcgaaca
     4201 cgtcggcgta atgccggtag aactcgttga ccgcctggca cacctcgcta tcgtcggcga
     4261 acttgcttgg gaggaaggac acgcccatgc catcggacag aatctgcttg tgcagcgggc
     4321 ggagcttggc gatcctctca gacttatggc agtgctggtt atggtgtgaa ttgatgagct
     4381 cgttaatgcc ctggatcttg cggctgccgg cctctcctga aatgccgccc aggagtgtat
     4441 tgtaggcggt gatgccctcc tgtgtcagga gcttatggta gccgtcgagg tgagacgcca
     4501 gtgagacatc gaggccgctg gctgtgagct cgttaatgat ctggtcgtac agcgcggaat
     4561 gcttctgctt aatggtggcc aggatctgga gattatcaat gaacctcggg aggttctcat
     4621 gaatcagccg gtacgcgatg gcggtgtgct tatcctcgtc agagtacata ttcttcctgt
     4681 tcctgtggaa gcctgtgaag taggttgaga acttctcgaa atgcgccagg tgggcgagct
     4741 tagggctgga ctctccctcc tgggcaatca cgaacttggc cagatcgccg agctccttgc
     4801 cgtccttgaa gagcttcgcg ccgaacagcc tatcgtagcc ggccttgtac ttgccgccat
     4861 tgccaatagg cttgacgatc tccttccgca gcacggcctg gaggtccttg agctgcttct
     4921 ggaggccatc gtcctttggg ttcttgcgga acttcaggta gacatcgtag aactccgcca
     4981 gcttggtgag cttcacctcg cccatcatat cggcaatgaa gtccctatgg taatcgtcca
     5041 ggatcgcctt gaccttctgg tacatatcgg ccattgtctc gtcctgattg aggaagttct
     5101 tggcatgaat gtgctcgagg gtcttgccga taggcttcag ctcgaaccgc actgtcttgg
     5161 acagtgggta gaggtgggtg aagtcctgga agagcatagc cgcaggcacc ccgtgaatac
     5221 caaccttccg cttcttctta ggagccatgg cggcctgcag gaagtaacac caaacaacag
     5281 ggtgagcatc gacaaaagaa acagtaccaa gcaaataaat agcgtatgaa ggcagggcta
     5341 aaaaaatcca catatagctg ctgcatatgc catcatccaa gtatatcaag atcaaaataa
     5401 ttataaaaca tacttgttta ttataataga taggtactca aggttagagc atatgaatag
     5461 atgctgcata tgccatcatg tatatgcatc agtaaaaccc acatcaacat gtatacctat
     5521 cctagatcga tatttccatc catcttaaac tcgtaactat gaagatgtat gacacacaca
     5581 tacagttcca aaattaataa atacaccagg tagtttgaaa cagtattcta ctccgatcta
     5641 gaacgaatga acgaccgccc aaccacacca catcatcaca accaagcgaa caaaaagcat
     5701 ctctgtatat gcatcagtaa aacccgcatc aacatgtata cctatcctag atcgatattt
     5761 ccatccatca tcttcaattc gtaactatga atatgtatgg cacacacata cagatccaaa
     5821 attaataaat ccaccaggta gtttgaaaca gaattaattc tactccgatc tagaacgacc
     5881 gcccaaccag accacatcat cacaaccaag acaaaaaaaa gcatgaaaag atgacccgac
     5941 aaacaagtgc acggcatata ttgaaataaa ggaaaagggc aaaccaaacc ctatgcaacg
     6001 aaacaaaaaa aatcatgaaa tcgatcccgt ctgcggaacg gctagagcca tcccaggatt
     6061 ccccaaagag aaacactggc aagttagcaa tcagaacgtg tctgacgtac aggtcgcatc
     6121 cgtgtacgaa cgctagcagc acggatctaa cacaaacacg gatctaacac aaacatgaac
     6181 agaagtagaa ctaccgggcc ctaaccatgg accggaacgc cgatctagag aaggtagaga
     6241 gggggggggg ggaggacgag cggcgtacct tgaagcggag gtgccgacgg gtggatttgg
     6301 gggagatctg gttgtgtgtg tgtgcgctcc gaacaacacg aggttgggga aagagggtgt
     6361 ggagggggtg tctatttatt acggcgggcg aggaagggaa agcgaaggag cggtgggaaa
     6421 ggaatccccc gtagctgccg gtgccgtgag aggaggagga ggccgcctgc cgtgccggct
     6481 cacgtctgcc gctccgccac gcaatttctg gatgccgaca gcggagcaag tccaacggtg
     6541 gagcggaact ctcgagaggg gtccagaggc agcgacagag atgccgtgcc gtctgcttcg
     6601 cttggcccga cgcgacgctg ctggttcgct ggttggtgtc cgttagactc gtcgacggcg
     6661 tttaacaggc tggcattatc tactcgaaac aagaaaaatg tttccttagt ttttttaatt
     6721 tcttaaaggg tatttgttta atttttagtc actttatttt attctatttt atatctaaat
     6781 tattaaataa aaaaactaaa atagagtttt agttttctta atttagaggc taaaatagaa
     6841 taaaatagat gtactaaaaa aattagtcta taaaaaccat taaccctaaa ccctaaatgg
     6901 atgtactaat aaaatggatg aagtattata taggtgaagc tatttgcaaa aaaaaaggag
     6961 aacacatgca cactaaaaag ataaaactgt agagtcctgt tgtcaaaata ctcaattgtc
     7021 ctttagacca tgtctaactg ttcatttata tgattctcta aaacactgat attattgtag
     7081 tactatagat tatattattc gtagagtaaa gtttaaatat atgtataaag atagataaac
     7141 tgcacttcaa acaagtgtga caaaaaaaat atgtggtaat tttttataac ttagacatgc
     7201 aatgctcatt atctctagag aggggcacga ccgggtcacg ctgcactgca ggcgcgccca
     7261 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     7321 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     7381 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
     7441 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca
     7501 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     7561 caaagcaagt ggattgatgt gaacatggtg gagcacgaca ctctcgtcta ctccaagaat
     7621 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
     7681 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
     7741 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
     7801 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     7861 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     7921 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     7981 tcatttcatt tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc
     8041 ctaggatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     8101 tcgacagcgt gtccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     8161 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     8221 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     8281 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     8341 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     8401 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     8461 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     8521 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     8581 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     8641 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     8701 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     8761 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     8821 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     8881 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     8941 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     9001 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgggat
     9061 cttggaggtg attaattaac gatcgacaag ctctattttc tccataataa tgtgtgagta
     9121 gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg agcatataag
     9181 aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct
     9241 aaaaccaaaa tccagtacta aaatccagat cccccgaatt aattcggcgt taattcagta
     9301 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     9361 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     9421 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     9481 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     9541 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     9601 gcctgtgatc accgcggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt
     9661 gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt
     9721 gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa
     9781 gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg
     9841 aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg
     9901 tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct
     9961 atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa
    10021 aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg
    10081 gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt
    10141 atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata
    10201 gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg
    10261 attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt
    10321 taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca
    10381 acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg
    10441 cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag
    10501 aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa
    10561 aatattatat tttactggat gaattgtttt agtacctaga atgcatgacc aaaatccctt
    10621 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    10681 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    10741 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    10801 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
    10861 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10921 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10981 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    11041 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    11101 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    11161 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    11221 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    11281 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    11341 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    11401 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
    11461 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
    11521 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
    11581 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgcttgtct gctcccggca
    11641 tccgcttaca gacaagctgt gaccgtttcc gggagctgca tgtgtcagag gttttcaccg
    11701 tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga
    11761 cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg
    11821 gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag
    11881 ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag
    11941 gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt
    12001 ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc
    12061 aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac
    12121 aggaaagagg ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc
    12181 cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag
    12241 gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc
    12301 gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc
    12361 gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg
    12421 gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac
    12481 gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat
    12541 gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc
    12601 ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg
    12661 cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc
    12721 tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc
    12781 tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac
    12841 caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc
    12901 gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa
    12961 aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac
    13021 gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc
    13081 cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg
    13141 gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac
    13201 caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac
    13261 gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcaggc aacccagccg
    13321 cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct
    13381 ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat
    13441 tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt
    13501 cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa
    13561 gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct
    13621 gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc
    13681 tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca
    13741 gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag
    13801 ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg
    13861 gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt
    13921 agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc
    13981 catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg
    14041 gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg
    14101 atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga
    14161 tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga
    14221 acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct
    14281 gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt
    14341 tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata
    14401 cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg
    14461 ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagccgt
    14521 gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa
    14581 tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc
    14641 attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg
    14701 ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga
    14761 gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag
    14821 ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc
    14881 tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa
    14941 ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc
    15001 ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc
    15061 cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc
    15121 agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat
    15181 cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa
    15241 gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt
    15301 catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc
    15361 gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg
//
