LOCUS       sequence-456265-       18631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The backbone vector for rice genome editing is based on PrCas12a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18631)
  AUTHORS   Liu S, He Y, Fan T, Zhu M, Qi C, Ma Y, Yang M, Yang L, Tang X, Zhou 
            J, Zhong Z, An X, Qi Y, Zhang Y
  TITLE     PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single 
            transcript unit systems for efficient singular and multiplexed 
            genome editing in rice, maize, and tomato.
  JOURNAL   Plant Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486.
  PUBMED    39387219
REFERENCE   2  (bases 1 to 18631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2024 Oct 10. doi: 10.1111/pbi.14486."
COMMENT     Sequence Label: pGEL1023
FEATURES             Location/Qualifiers
     source          1..18631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1189..1818
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2247..3320
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3386..3580
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3816..3838)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3924..4064
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3979..3998
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4079..4096)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4250..4838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4330..4349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6263..7057)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7482..7506
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    7584..7758
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7799..8824)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             complement(7799..8824)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8897..9242)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8934..8954)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9433..9454
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9469..9474,9475..9492,9493..9499)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9507..9523
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9512..9534
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9531..9547
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9531..9547
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     regulatory      11586..11595
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11598..11618
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             15279..15326
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      15345..15593
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     ncRNA           17157..17193
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             complement(17221..17526)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(17323..17342)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     ncRNA           17853..17920
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(18276..18293)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(18276..18292)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18285..18307)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    18495..18519
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
       61 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
      121 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
      181 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
      241 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
      301 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
      361 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
      421 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
      481 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
      541 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
      601 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
      661 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
      721 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
      781 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
      841 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
      901 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
      961 cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc
     1021 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     1081 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     1141 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     1201 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     1261 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     1321 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     1381 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     1441 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     1501 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     1561 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     1621 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     1681 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     1741 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     1801 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     1861 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     1921 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     1981 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     2041 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     2101 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     2161 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     2221 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     2281 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     2341 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     2401 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     2461 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     2521 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     2581 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     2641 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     2701 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     2761 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     2821 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     2881 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     2941 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     3001 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     3061 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     3121 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     3181 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     3241 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     3301 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     3361 aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     3421 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     3481 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     3541 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     3601 cccaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     3661 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     3721 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     3781 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggaa
     3841 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     3901 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     3961 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4021 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggccctct tccgcttcct
     4081 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4141 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4681 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4861 cggggtctga cgctcagtgc tgagagatcc cctcataatt tccccaaagc gtaaccatgt
     4921 gtgaataaat tttgagctag tagggttgca gccacgagta agtcttccct tgttattgtg
     4981 tagccagaat gccgcaaaac ttccatgcct aagcgaactg ttgagagtac gtttcgattt
     5041 ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt tctgagcatg aacgcccgca
     5101 agccaacatg ttagttgaag catcagggcg attagcagca tgatatcaaa acgctctgag
     5161 ctgctcgttc ggctatggcg taggcctagt ccgtaggcag gacttttcaa gtctcggaag
     5221 gtttcttcaa tctgcattcg cttcgaatag atattaacaa gttgtttggg tgttcgaatt
     5281 tcaacaggta agttagttgc tagaatccat ggctcctttg ccgacgctga gtagatttta
     5341 ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat tttttcggcc tttagagcga
     5401 gatttataca atagaatttg gcatgagatt ggattgcttt tagtcagcct cttatagcct
     5461 aaagtctttg agtgactaga tgacatatca tgtaagttgc tgataggttt ccagttttcc
     5521 gctcctaggt ctgcatattg tacttttcct cttactcgac ttaaccagta ccaacccagc
     5581 ttctcaacgg atttatacca tggcacttta aagccagcat cactgacaat gagcggtgtg
     5641 gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt ggtcatgagc tttctttgaa
     5701 cattgctctg aaagcgggaa cgctttctca taaagagtaa cagaacgacc gtgtagtgcg
     5761 actgaagctc gcaataccat aagccgtttt tgctcacgga tatcagacca gtcaacaagt
     5821 acaatgggca tcgtattgcc cgaacagata aagctagcat gccaacggta tacagcgagt
     5881 cgctctttgt ggaggtgacg attacctaac aatcggtcga ttcgtttgat gttatgtttt
     5941 gttctcgctt tggttggcag gttacggcca agttcggtaa gagtgagagt tttacagtca
     6001 agtaaggcgt ggcaagccaa cgttaagctg ttgagtcgtt ttaagtgtaa ttcggggcag
     6061 aattggtaaa gagagtcgtg taaaatatcg agttcgcaca ttttgttgtc tgattattga
     6121 tttttggcga aaccatttga tcatatgaca agatgtgtat ctaccttaac ttaatgattt
     6181 tgataaaaat cattagggga ttcatcagcg ctcagtggaa cgaaaactca cgttaaggga
     6241 ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa tattttattt
     6301 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
     6361 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
     6421 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
     6481 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
     6541 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
     6601 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
     6661 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
     6721 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
     6781 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
     6841 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg
     6901 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
     6961 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
     7021 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
     7081 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
     7141 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
     7201 ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac
     7261 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
     7321 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
     7381 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
     7441 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
     7501 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga
     7561 attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat
     7621 tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
     7681 tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca
     7741 cacattatta tggagaaact cgagcttgtc gatcgacaga tcccggtcgg catctactct
     7801 atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
     7861 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
     7921 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
     7981 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
     8041 gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
     8101 tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
     8161 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
     8221 tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
     8281 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
     8341 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
     8401 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
     8461 tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt
     8521 cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt
     8581 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
     8641 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
     8701 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaca
     8761 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
     8821 tcatatctca ttgccccccg gatctgcgaa agctcgagag agatagattt gtagagagag
     8881 actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaagggt
     8941 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
     9001 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     9061 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     9121 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     9181 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     9241 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     9301 cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg
     9361 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     9421 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     9481 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     9541 ctatgacatg attacgaatt cgagctcggt acccggggat cggcgcgcct gcagtgcagc
     9601 gtgacccggt cgtgcccctc tctagagata atgagcattg catgtctaag ttataaaaaa
     9661 ttaccacata ttttttttgt cacacttgtt tgaagtgcag tttatctatc tttatacata
     9721 tatttaaact ttactctacg aataatataa tctatagtac tacaataata tcagtgtttt
     9781 agagaatcat ataaatgaac agttagacat ggtctaaagg acaattgagt attttgacaa
     9841 caggactcta cagttttatc tttttagtgt gcatgtgttc tccttttttt ttgcaaatag
     9901 cttcacctat ataatacttc atccatttta ttagtacatc catttagggt ttagggttaa
     9961 tggtttttat agactaattt ttttagtaca tctattttat tctattttag cctctaaatt
    10021 aagaaaacta aaactctatt ttagtttttt tatttaataa tttagatata aaatagaata
    10081 aaataaagtg actaaaaatt aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac
    10141 atttttcttg tttcgagtag ataatgccag cctgttaaac gccgtcgacg agtctaacgg
    10201 acaccaacca gcgaaccagc agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc
    10261 tctgtcgctg cctctggacc cctctcgaga gttccgctcc accgttggac ttgctccgct
    10321 gtcggcatcc agaaattgcg tggcggagcg gcagacgtga gccggcacgg caggcggcct
    10381 cctcctcctc tcacggcacc ggcagctacg ggggattcct ttcccaccgc tccttcgctt
    10441 tcccttcctc gcccgccgta ataaatagac accccctcca caccctcttt ccccaacctc
    10501 gtgttgttcg gagcgcacac acacacaacc agatctcccc caaatccacc cgtcggcacc
    10561 tccgcttcaa ggtacgccgc tcgtcctccc ccccccccct ctctaccttc tctagatcgg
    10621 cgttccggtc catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc
    10681 gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac
    10741 acgttctgat tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc
    10801 gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg gtttggtttg
    10861 cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc ttttcatgct
    10921 tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag
    10981 aattaattct gtttcaaact acctggtgga tttattaatt ttggatctgt atgtgtgtgc
    11041 catacatatt catagttacg aattgaagat gatggatgga aatatcgatc taggataggt
    11101 atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt tcgcttggtt
    11161 gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt agaatactgt
    11221 ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca tacatcttca
    11281 tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca tgttgatgtg
    11341 ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg ctctaacctt
    11401 gagtacctat ctattataat aaacaagtat gttttataat tattttgatc ttgatatact
    11461 tggatgatgg catatgcagc agctatatgt ggattttttt agccctgcct tcatacgcta
    11521 tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg tgttacttcc
    11581 tgcaggccgc catggctcct aagaagaagc ggaaggttgg tattcacggg gtgcctgcgg
    11641 ctatcattgg ccgggatttc aatatgtact accagaacct caccaagatg tacccaatct
    11701 ccaagacact caggaacgag ctgattcctg tgggcaagac cctggagaat atccgcaaga
    11761 acggcattct ggaggccgac atccagcgga aggcggatta cgagcacgtg aagaagctca
    11821 tggacaacta ccataagcag ctcatcaacg aggccctcca gggcgtccac ctctcagacc
    11881 tgtctgatgc gtacgacctc tacttcaacc tgtccaagga gaagaatagc gtggatgcct
    11941 tctcaaagtg ccaggacaag ctccgcaagg agatcgtcag cctcctgaag aaccatgaga
    12001 atttcccaaa gattggcaac aaggagatca ttaagctcct gcagtcactc tacgacaatg
    12061 ataccgacta caaggcgctg gactccttca gcaacttcta cacatacttc tccagctaca
    12121 atgaggtgag gaagaacctc tactccgacg aggagaagtc atctacagtc gcctaccgcc
    12181 tgatcaacga gaatctccct aagttcctgg acaacattaa ggcctacgcg atcgccaaga
    12241 aggccggcgt gcgcgccgag ggcctctcag aggaggatca ggactgcctg ttcatcattg
    12301 agacattcga gcggaccctc acacaggatg gcattgacaa ctacaatgcc gcgatcggca
    12361 agctcaacac agcgattaac ctgttcaatc agcagaacaa gaagcaggag ggcttccgga
    12421 aggtccctca gatgaagtgc ctctacaagc agatcctgtc tgatagggag gaggccttca
    12481 ttgacgagtt ctccgacgat gaggatctca tcaccaatat tgagagcttc gcggagaaca
    12541 tgaatgtgtt cctgaactca gagatcatta cagacttcaa gatcgccctc gtcgagtccg
    12601 atggcagcct ggtgtacatt aagaatgacg tgtccaagac ctccttctcc aacatcgtgt
    12661 tcggctcctg gaatgccatt gatgagaagc tctccgacga gtacgacctg gcgaactcca
    12721 agaagaagaa ggacgagaag tactacgaga agcgccagaa ggagctgaag aagaacaagt
    12781 cctacgacct ggagacaatc atcggcctgt tcgacgataa ctctgatgtg atcggcaagt
    12841 acattgagaa gctggagtcc gacatcacag cgattgccga ggcgaagaat gatttcgacg
    12901 agatcgtcct caggaagcac gacaagaaca agagcctgcg caagaacacc aatgccgtgg
    12961 aggcgattaa gtcatacctc gatacagtca aggacttcga gagggatatc aagctgatta
    13021 atggctctgg ccaggaggtg gagaagaacc tcgtggtcta cgccgagcag gagaacatcc
    13081 tggcggagat taagaatgtc gattctctct acaatatgtc caggaactac ctgacccaga
    13141 agcctttctc cacagagaag ttcaagctca acttcaatcg cgcgaccctc ctgaatggct
    13201 gggacaagaa caaggagaca gataatctcg gcatcctgtt cgagaaggat ggcatgtact
    13261 acctcggcat tatgaatacc aaggccaaca agatcttcgt gaacattccg aaggcgacat
    13321 ccaatgacgt gtaccacaag gtcaactaca agctcctgcc gggcccaaat aagatgctcc
    13381 cgaaggtgtt cttcgcccag tctaacctgg actactacaa gccatccgag gagctgctgg
    13441 ccaagtacaa ggcgggcacc cacaagaagg gcgacaactt ctccctggag gactgccatg
    13501 ccctgatcga cttcttcaag gcgtccattg agaagcatcc agattggtcc agcttcggct
    13561 tcgagttcag cgagacatgc acatacgagg acctctcagg cttctacagg gaggtggaga
    13621 agcagggcta caagatcacc tacacagatg tggacgccga ttacattacc agcctggtcg
    13681 agagggacga gctgtacctg ttccagatct acaataagga cttctcaccg tactctaagg
    13741 gcaacctcaa tctgcacaca atctacctcc agatgctgtt cgatcagcgc aatctcaaca
    13801 atgtggtcta caagctgaac ggcgaggcgg aggtgttcta caggccagcg agcatcaacg
    13861 atgaggaggt catcattcac aaggccggcg aggagattaa gaacaagaat tctaagaggg
    13921 cggtggacaa gccaacctcc aagttcggct acgacatcat taaggatagg cgctactcaa
    13981 aggataagtt catgctccat atccctgtga ccatgaactt cggcgtcgac gagacacgga
    14041 ggttcaatga cgtggtcaac gatgcgctgc ggaatgatga gaaggtgagg gtcatcggca
    14101 ttgacagggg cgagcgcaac ctcctctacg tggtcgtggt cgacaccgat ggcacaatcc
    14161 tggagcagat ttccctgaat agcatcatta acaatgagta ctctatcgag acagattacc
    14221 ataagctcct ggacgagaag gagggcgacc gcgatcgggc caggaagaac tggaccacaa
    14281 tcgagaatat taaggagctg aaggagggct acctgtccca ggtggtcaac gtgatcgcca
    14341 agctcgtcct gaagtacaat gcgatcattt gcctggagga cctcaacttc ggcttcaagc
    14401 gcggccggca gaaggtggag aagcaggtct accagaagtt cgagaagatg ctcatcgaca
    14461 agctcaatta cctcgtgatc gataagtccc gcaagcagga caagccagag gagttcggcg
    14521 gcgccctgaa cgcgctccag ctgacctcaa agttcacatc tttcaaggat atgggcaagc
    14581 agaccggcat catctactac gtccctgcct acctcacatc caagatcgac ccgaccacag
    14641 gcttcgcgaa tctgttctac gtgaagtacg agaacgtcga gaaggccaag gagttcttca
    14701 gcaggttcga ttccatcagc tacaacaatg agtcaggcta cttcgagttc gccttcgatt
    14761 acaagaagtt caccgacagg gcatgcggcg cgcggagcca gtggaccgtg tgcacatacg
    14821 gcgagaggat cattaagttc cgcaataccg agaagaacaa tagcttcgac gataagacaa
    14881 tcgtcctctc agaggagttc aaggagctgt tcagcatcta cggcatttca tacgaggacg
    14941 gcgccgagct gaagaacaag atcatgtctg tggatgaggc ggacttcttc cggtccctca
    15001 ccaggctgtt ccagcagaca atgcagatgc gcaattcatc taacgacgtc acccgggatt
    15061 acatcatttc ccctatcatg aatgatcggg gcgagttctt caactctgag gcctgcgacg
    15121 cgtccaagcc aaaggacgcc gatgcgaatg gcgccttcaa tatcgccagg aagggcctct
    15181 gggtgctgga gcagattagg aatacaccaa gcggcgacaa gctcaacctg gccatgtcaa
    15241 atgcggagtg gctggagtac gcccagagga accagatcaa gcgtcctgct gccaccaaaa
    15301 aggccggaca ggctaagaaa aagaagtgaa cgcgtactcg agtatatgaa gatgaagatg
    15361 aaatatttgg tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct
    15421 tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaagat cacattataa
    15481 tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc ttctgcagca
    15541 tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat aaggagctcg
    15601 gacggcgcgc ccacatcagt ctctgcacaa agtgcatcct gggctgcttc aattataaag
    15661 ccccattcac cacatttgct agatagtcga aaagcaccat caatattgag cttcaggtat
    15721 ttttggttgt gttgtggttg gattgattct aatatatacc aaatcaatat aattcactac
    15781 caaaatatac catagccatc acaactttat taattttggt agcttaagat ggtatatata
    15841 ataaccaatt aacaactgat tctaatttta ctacggccca gtatctacca atacaaaaca
    15901 acgagtatgt tttcttccgt cgtaatcgta cacagtacaa aaaaacctgg ccagcctttc
    15961 ttgggctggg gctctctttc gaaaggtcac aaaacgtaca cggcagtaac gccgcttcgc
    16021 tgcgtgttaa cggccaccaa ccccgccgtg agcaaacggc atcagctttc cacctcctcg
    16081 atatctccgc ggcgccgtct ggacccgccc cctttccgtt cctttctttc cttctcgcgt
    16141 ttgcgtggtg gggacggact ccccaaaccg cctctccctc tctttatttg tctatattct
    16201 cactgggccc cacccaccgc acccctgggc ccactcacga gtccccccct ccccacctat
    16261 aaatacccca ccccctcctc gcctcttcct ccatcaatcg aatccccaaa atcgcagaga
    16321 aaaaaaaatc tcccctcgaa gcgaagcgtc gaatcgcctt ctcaaggtat gcgattttct
    16381 gatcctctcc gttcctcgcg tttgatttga tttcccggcc tgttcgtgat tgtgagatgt
    16441 tgttgttagt ctccgttttg cgatctgtgg tagatttgaa cagggttaga tggggttcgc
    16501 gtggtatgct ggatctgtga ttatgagcga tgctgttcgt ggtccaagta tttattggtt
    16561 cggatctaga agtagaactg tgctagggtt gtgatttgtt ccgatctgtt caatcagtag
    16621 gatttagtct ctgtttttct cgttgatcca agtagcagct tcaggtatat tttgcttagg
    16681 ttgtttttga ttcagtccct ctagttgcat agattctact ctgttcatgt ttaatctaag
    16741 ggctgcgtct tgttgattag tgattacata gcatagcttt caggatattt tacttgctta
    16801 tgcctatctt atcaactgtt gcacctgtaa attctagcct atgttaatta acctgcctta
    16861 tgtgctctcg ggatagtgct agtagttatt gaatcagttt gccgatggaa ttctagtagt
    16921 tcatagacct gcagattatt tttgtgaact cgagcacggt gcgactctct attttgttag
    16981 gtcactgttg gtgttgatag gtacactgat gttattgtgg tttagatcgt gtatctaaca
    17041 tattggaata atttgattga ctgatttctg ctgtacttgc ttggtattgt tataatttca
    17101 tgttcatagt tgctgaccat gcttcggtaa ttgtgtgtgc agcctgcagg aaattactga
    17161 tgagtccgtg aggacgaaac gagtaagctc gtctaatttc tactatggta gatggagacc
    17221 ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag
    17281 atcagccact tcttccccga taacggaaac cggcacactg gccatatcgg tggtcatcat
    17341 gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga
    17401 cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc
    17461 actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa
    17521 ctgcatttca ccagcccctg ttctcgtcag caaaagagcc gttcatttca ataaaccggg
    17581 cgacctcagc catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg
    17641 cttcattctg catggttgtg cttaccagac cggagatatt gacatcatat atgccttgag
    17701 caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag catacctctt
    17761 tttgacatac ttcgggtata catatcagta tatattctta taccgcaaaa atcagcgcgc
    17821 aaatacgcat actgttatct ggcttggtct caggccggca tggtcccagc ctcctcgctg
    17881 gcgccggctg ggcaacatgc ttcggcatgg cgaatgggac actcgagtcc tagacttgtc
    17941 catcttctgg attggccaag ttaattaatg tatgaaataa aaggatgcac acatagtgac
    18001 atgctaatca ctataatgtg ggcatcaaag ttgtgtgtta tgtgtaatta ctaattatct
    18061 gaataagaga aagagatcat ccatatttct tatcctaaat gaatgtcacg tgtctttata
    18121 attctttgat gaaccagatg cattttatta accaattcca tatacatata aatattaatc
    18181 atatataatt aatatcaatt gggttagcaa aacaaatcta gtctaggtgt gttttgcgag
    18241 ctcccggggc gcgccgatat cgagctcagt gtggcactgg ccgtcgtttt acaacgtcgt
    18301 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    18361 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    18421 aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg ccttcagttt
    18481 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
    18541 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
    18601 gcatgccaac cacagggttc ccctcgggat c
//
