LOCUS       sequence-456273-        8158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Hung YP, Albeck JG, Tantama M, Yellen G
  TITLE     Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded
            Fluorescent Biosensor.
  JOURNAL   Cell Metab. 2011 Oct 5;14(4):545-54.
  PUBMED    21982714
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Metab."; date: "2011-10-5"; volume: "14(4)"; pages: "545-54"
COMMENT     Sequence Label: pcDNA3.1-Peredox-mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..85
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        66..84
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(133..744,745..765,766..1500,1501..1503,1504..2133,
                     2134..2178)
                     /codon_start=1
                     /product="genetically encoded fluorescent biosensor of the 
                     cytosolic NADH-NAD+ redox state (Hung et al., 2011)"
                     /label=Peredox
                     /note="consists of two pH-insensitive mutant copies of the 
                     Thermus aquaticus redox-sensing repressor Rex (T-Rex) 
                     separated by circularly permuted monomeric T-Sapphire 
                     (cpmTS), a pH-resistant GFP variant"
                     /translation="KVPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVD
                     KDLSYFGSYGTDGVGYTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESF
                     ELRGFFDVDPGMVGRPVRGGVIEHVDLLPQRVPGRIEIALLTVPREAAQKAADLLVAAG
                     IKGILNFAPVVLEVPKEVAVENVDILAGLTRLSFAILNPTWSAAGGHGFTAHNVYIMAD
                     KQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSKLSKDPNEK
                     RDHMVLLEFVTAAGITHGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFS
                     VSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSA
                     MPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTK
                     VPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVDEDLSYFGSYGTDGVG
                     YTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESFELRGFFDVDPGMVGR
                     PVRGGVIEHVDLLPQRVPGRIEIALLTVPREAAQKAADLLVAAGIKGILNFAPVVLEVP
                     KEVAVENVDFLAGLTRLSFAILNPKWREEMMGSGTGGNASDGGGSGG"
     CDS             2179..2886
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2324..2342)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2592..2611
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2887..2907
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2952..2969)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2958..3182
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3228..3656
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3315..3334)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3525..3546
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3665..3685)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3670..3999
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3850..3985
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3912..3931
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4066..4860
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4120..4139)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4730..4749
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5034..5155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5071..5090)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5125..5144
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5204..5220)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5204..5220)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5217..5239)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5228..5244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5252..5258,5259..5276,5277..5282))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5297..5318
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5435..5452)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5606..6194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5686..5705)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6365..7156,7157..7225))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6988..7007
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7226..7330)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7405..7424)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7596..7975
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(7976..8158,1..21)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8130..8150
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
       61 gaaattaata cgactcacta tagggagacc caagctggct agctctagat tgggcccacc
      121 atggttgcta gcaaagttcc tgaagcagcc atttccagac tgattactta cctgagaatt
      181 ctggaagagc tggaagccca aggtgtacac cgcaccgcct ccgagcaact gggcgagctg
      241 gcccaggtca ccgccttcca ggttgataag gacctgtcct acttcggctc ctacggcact
      301 gacggcgttg gctacactgt accggtcctc aagagagaac tgcgccatat cctcggtctg
      361 aaccgcaaat ggggcctgtg catcgtgggg atgggccgcc tgggatccgc tcttgctgat
      421 tggcctggtt ttggcgagag cttcgagctg cgcggtttct tcgatgttga cccaggtatg
      481 gttggtcggc cggttcgcgg tggtgttatc gaacacgttg atctgctgcc ccagcgcgta
      541 cctggtcgca tcgaaatcgc tctgctgacg gttccacgcg aggcagcaca gaaggctgcc
      601 gacctgctgg ttgccgctgg catcaagggc atcctgaact tcgctccagt tgtactggag
      661 gttcccaagg aagtggctgt tgagaacgtt gacatcctgg ccggtctgac gcgtctgagc
      721 tttgccattc tgaaccccac gtggagcgca gcaggtgggc atggtttcac tgctcacaac
      781 gtgtatatca tggctgacaa acagaagaac ggtatcaagg ccaacttcaa gatccgccac
      841 aacatcgaag acggcggcgt tcagctggct gatcactacc agcagaacac cccaatcggc
      901 gacggccctg ttctgctgcc tgacaaccac tacttaagca tccagtctaa actgtccaaa
      961 gacccaaacg agaagcgcga ccacatggtt ctgctggaat ttgttaccgc cgctggcatc
     1021 acccacggca tggacgaact gtacaagggc ggcaccgggg gatctatggt ttccaaaggc
     1081 gaggaactgt tcactggtgt tgttcctatc ctggttgaac tggacggcga cgttaacggc
     1141 cacaaattca gcgttagcgg ggaaggcgaa ggagacgcta cctacggcaa gctgactctg
     1201 aagttcatct gcaccactgg caagctgcct gttccttggc ctaccctggt tactaccttc
     1261 tcctatggtg ttatggtttt cgccagatat cctgaccaca tgaagcagca cgacttcttc
     1321 aagagcgcca tgcctgaggg ctacgttcag gaacgcacca tctttttcaa ggacgacggc
     1381 aactataaga cacgcgctga ggttaagttc gagggcgaca ctctggttaa ccgcatcgag
     1441 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg gccataagct tgaatataac
     1501 accaaggtac ctgaagccgc catcagccgc ctcatcactt acctccgcat cctggaggag
     1561 ctcgaagccc aaggcgttca ccgcactgca agcgagcagc tgggcgaact ggcacaggtt
     1621 acagcattcc aggttgacga agatctgtcc tatttcggtt cctacggcac cgacggtgtt
     1681 ggctacacag tccctgtcct gaagcgcgag ctgcgccaca tcctgggcct gaaccgcaag
     1741 tggggcttat gcatcgttgg catgggccgc ctcggtagtg cactggcaga ctggccgggc
     1801 tttggtgaat ctttcgaact gcgcggcttc ttcgacgttg atccaggcat ggttgggcgc
     1861 cctgttcgcg gtggcgttat cgagcatgtt gacctcctgc cacagcgcgt cccgggtcgc
     1921 atcgagatcg ccctgctgac cgttcctcgc gaagctgcac agaaggcagc cgacttacta
     1981 gtcgccgctg gtatcaaggg aattctgaac ttcgcccctg ttgttctgga ggttcctaag
     2041 gaagttgctg ttgaaaacgt tgatttcctg gccggcctga cccgcctgag cttcgcaatc
     2101 ctgaatccta agtggcgcga agaaatgatg ggttccggaa ctgggggcaa cgcctctgac
     2161 ggtggtgggt ctggtggtat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     2221 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     2281 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     2341 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     2401 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     2461 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     2521 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     2581 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     2641 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     2701 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     2761 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     2821 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgaactg
     2881 tacaagccta aaaagaaacg taaagttgaa gatgcttgag tcgagaagct taagtttaaa
     2941 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3001 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3061 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3121 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     3181 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     3241 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3301 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3361 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3421 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3481 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3541 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3601 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     3661 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3721 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3781 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3841 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3901 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     3961 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     4021 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     4081 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     4141 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4201 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     4261 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     4321 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     4381 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     4441 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     4501 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4561 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     4621 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     4681 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     4741 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     4801 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     4861 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     4921 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     4981 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     5041 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5101 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5161 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     5221 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     5281 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     5341 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     5401 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5461 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5521 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5581 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5641 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5701 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5761 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5821 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5881 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5941 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6001 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6061 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6121 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6181 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6241 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6301 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6361 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6421 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6481 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6541 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6601 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6661 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6721 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6781 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6841 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6901 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6961 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7021 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7081 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7141 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7201 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7261 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7321 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     7381 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     7441 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     7501 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     7561 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
     7621 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     7681 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     7741 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     7801 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     7861 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     7921 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     7981 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     8041 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     8101 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtgg
//
