LOCUS       sequence-456465-        5991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Shimizu S, Khan MZ, Hippensteel RL, Parkar A, Raghupathi R, Meucci O
  TITLE     Role of the transcription factor E2F1 in CXCR4-mediated 
            neurotoxicity and HIV neuropathology.
  JOURNAL   Neurobiol Dis. 2007 Jan . 25(1):17-26.
  PUBMED    17011204
REFERENCE   2  (bases 1 to 5991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurobiol 
            Dis. 2007 Jan . 25(1):17-26."
COMMENT     Sequence Label: pMax-E2F1
FEATURES             Location/Qualifiers
     source          1..5991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..1248
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     polyA_signal    2719..2840
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2756..2775)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2810..2829
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2847..3302)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2984..3005)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3196..3215
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3329..3433
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3435..3792
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3435..3626
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3458..3478)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3643..3778
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3827..4621
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3827..4621
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3881..3900)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4491..4510
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4809..4828)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4853..4900
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5229..5817
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5718..5737
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
      601 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
      661 gagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
      721 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
      781 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
      841 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
      901 gtgatcggcg acttcaaggt gatgggcacc ggcttccccg aggacagcgt gatcttcacc
      961 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgat
     1021 ctggatggca gcttcacccg caccttcagc ctgcgcgacg gcggctacta cagctccgtg
     1081 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     1141 cccatgttcg ccttccgccg cgtggaggag gatcacagca acaccgagct gggcatcgtg
     1201 gagtaccagc acgccttcaa gaccccggat gcagatgccg gtgaagaaag atctcgagct
     1261 caagcttcga attccatggc cttggccggg gcccctgcgg gcggcccatg cgcgccggcg
     1321 ctggaggccc tgctcggggc cggcgcgctg cggctgctcg actcctcgca gatcgtcatc
     1381 atctccgccg cgcaggacgc cagcgccccg ccggctccca ccggccccgc ggcgcccgcc
     1441 gccggcccct gcgaccctga cctgctgctc ttcgccacac cgcaggcgcc ccggcccaca
     1501 cccagtgcgc cgcggcccgc gctcggccgc ccgccggtga agcggaggct ggacctggaa
     1561 actgaccatc agtacctggc cgagagcagt gggccagctc ggggcagagg ccgccatcca
     1621 ggaaaaggtg tgaaatcccc gggggagaag tcacgctatg agacctcact gaatctgacc
     1681 accaagcgct tcctggagct gctgagccac tcggctgacg gtgtcgtcga cctgaactgg
     1741 gctgccgagg tgctgaaggt gcagaagcgg cgcatctatg acatcaccaa cgtccttgag
     1801 ggcatccagc tcattgccaa gaagtccaag aaccacatcc agtggctggg cagccacacc
     1861 acagtgggcg tcggcggacg gcttgagggg ttgacccagg acctccgaca gctgcaggag
     1921 agcgagcagc agctggacca cctgatgaat atctgtacta cgcagctgcg cctgctctcc
     1981 gaggacactg acagccagcg cctggcctac gtgacgtgtc aggaccttcg tagcattgca
     2041 gaccctgcag agcagatggt tatggtgatc aaagcccctc ctgagaccca gctccaagcc
     2101 gtggactctt cggagaactt tcagatctcc cttaagagca aacaaggccc gatcgatgtt
     2161 ttcctgtgcc ctgaggagac cgtaggtggg atcagccctg ggaagacccc atcccaggag
     2221 gtcacttctg aggaggagaa cagggccact gactctgcca ccatagtgtc accaccacca
     2281 tcatctcccc cctcatccct caccacagat cccagccagt ctctactcag cctggagcaa
     2341 gaaccgctgt tgtcccggat gggcagcctg cgggctcccg tggacgagga ccgcctgtcc
     2401 ccgctggtgg cggccgactc gctcctggag catgtgcggg aggacttctc cggcctcctc
     2461 cctgaggagt tcatcagcct ttccccaccc cacgaggccc tcgactacca cttcggcctc
     2521 gaggagggcg agggcatcag agacctcttc gactgtgact ttggggacct cacccccctg
     2581 gatttctgag gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     2641 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     2701 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2761 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2821 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     2881 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     2941 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     3001 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     3061 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     3121 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     3181 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     3241 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     3301 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3361 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3421 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     3481 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3541 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3601 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3661 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3721 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3781 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     3841 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     3901 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     3961 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     4021 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4081 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4141 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4201 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4261 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4321 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     4381 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4441 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     4501 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     4561 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     4621 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     4681 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     4741 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     4801 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     4861 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     4921 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     4981 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     5041 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     5101 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5161 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5221 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5281 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5341 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5401 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5461 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5521 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5581 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5641 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5701 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5761 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5821 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     5881 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta
     5941 atagtaatca attacggggt cattagttca tagcccatat atggagttcc g
//
