LOCUS       sequence-456508-       10005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag PTK2
FEATURES             Location/Qualifiers
     source          1..10005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        149..528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        529..732
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     683..703
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    953..1152
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1353..1769
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1699..1721
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1737..1753
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1787..1796
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1793..1834
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1859..1882
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1889..1913
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4036..4497
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4092..4109)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4319..4338
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4502..5296
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4556..4575)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5166..5185
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5483..5952
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        5988..6092
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6093..6161,6162..6953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6311..6330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8719..9307
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9208..9227
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9461..9478
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9582..10005,1..4))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9692..9713)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9904..9923
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgcgccgatc ttcaatattg gccattagcc atattattca ttggttatat agcataaatc
       61 aatattggct attggccatt gcatacgttg tatctatatc ataatatgta catttatatt
      121 ggctcatgtc caatatgacc gccatgttgg cattgattat tgactagtta ttaatagtaa
      181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      541 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      661 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      721 ataagcagag ctggtttagt gaaccgcgcc agtcctccga ttgactgagt cgcccgggta
      781 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
      841 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg
      901 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gcaagctggc
      961 cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc
     1021 gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt
     1081 tctgaacacc cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg
     1141 gcccgacctg aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg
     1201 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga
     1261 accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc
     1321 tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt
     1381 tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca
     1441 agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc
     1501 cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac
     1561 ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt
     1621 ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc
     1681 catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt
     1741 atccagccct cactccttct ctaggcgccg gccggatcca gaattcgcca ccatggggtc
     1801 ttcaaaatct aaaccaaagg accccagcca gcgccggcgc aggatccgag gttaccttga
     1861 ctacaaagac gatgacgaca agcaattgac aagtttgtac aaaaaagttg gcatgaaata
     1921 ccaggaggtc agatgtttaa cctctttcaa catatctgtt tcttttcctg cagaaacaga
     1981 tgattatgct gagattatag atgaagaaga tacttacacc atgccctcaa ccagggatta
     2041 tgagattcaa agagaaagaa tagaacttgg acgatgtatt ggagaaggcc aatttggaga
     2101 tgtacatcaa ggcatttata tgagtccaga gaatccagct ttggcggttg caattaaaac
     2161 atgtaaaaac tgtacttcgg acagcgtgag agagaaattt cttcaagaag ccttaacaat
     2221 gcgtcagttt gaccatcctc atattgtgaa gctgattgga gtcatcacag agaatcctgt
     2281 ctggataatc atggagctgt gcacacttgg agagctgagg tcatttttgc aagtaaggaa
     2341 atacagtttg gatctagcat ctttgatcct gtatgcctat cagcttagta cagctcttgc
     2401 atatctagag agcaaaagat ttgtacacag ggacattgct gctcggaatg ttctggtgtc
     2461 ctcaaatgat tgtgtaaaat taggagactt tggattatcc cgatatatgg aagatagtac
     2521 ttactacaaa gcttccaaag gaaaattgcc tattaaatgg atggctccag agtcaatcaa
     2581 ttttcgacgt tttacctcag ctagtgacgt atggatgttt ggtgtgtgta tgtgggagat
     2641 actgatgcat ggtgtgaagc cttttcaagg agtgaagaac aatgatgtaa tcggtcgaat
     2701 tgaaaatggg gaaagattac caatgcctcc aaattgtcct cctaccctct acagccttat
     2761 gacgaaatgc tgggcctatg accccagcag gcggcccagg tttactgaac ttaaagctca
     2821 gctcagcaca atcctggagg aagagaaggc tcagcaagaa gagcgcatga ggatggagtc
     2881 cagaagacag gccacagtgt cctgggactc cggagggtct gatgaagcac cgcccaagcc
     2941 cagcagaccg ggttatccca gtccgaggtc cagcgaagga ttttatccca gcccacagca
     3001 catggtacaa accaatcatt accaggtttc tggctaccct ggttcacatg gaatcacagc
     3061 catggctggc agcatctatc caggtcaggc atctcttttg gaccaaacag attcatggaa
     3121 tcatagacct caggagatag caatgtggca gcccaatgtg gaggactcta cagtattgga
     3181 cctgcgaggg attgggcaag tgttgccaac ccatctgatg gaagagcgtc taatccgaca
     3241 gcaacaggaa atggaagaag atcagcgctg gctggaaaaa gaggaaagat ttctgaaacc
     3301 tgatgtgaga ctctctcgag gcagtattga cagggaggat ggaagtcttc agggtccgat
     3361 tggaaaccaa catatatatc agcctgtggg taaaccagat cctgcagctc caccaaagaa
     3421 accgcctcgc cctggagctc ccggtcatct gggaagcctt gccagcctca gcagccctgc
     3481 tgacagctac aacgagggtg tcaagcttaa gccccaggaa atcagccccc ctcctactgc
     3541 caacctggac cggtcgaatg ataaggtgta cgagaatgtg acgggcctgg tgaaagctgt
     3601 catcgagatg tccagtaaaa tccagccagc cccaccagag gagtatgtcc ctatggtgaa
     3661 ggaagtcggc ttggccctga ggacattatt ggccactgtg gatgagacca ttcccctcct
     3721 accagccagc acccaccgag agattgagat ggcacagaag ctattgaact ctgacctggg
     3781 tgagctcatc aacaagatga aactggccca gcagtatgtc atgaccagcc tccagcaaga
     3841 gtacaaaaag caaatgctga ctgctgctca cgccctggct gtggatgcca aaaacttact
     3901 cgatgtcatt gaccaagcaa gactgaaaat gcttgggcag acgagacgac actgccaact
     3961 ttcttgwaca aagtggtttg atctagtcga ccaattccgg ttattttcca ccatattgcc
     4021 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     4081 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     4141 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     4201 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     4261 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     4321 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     4381 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     4441 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatac
     4501 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4561 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4621 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4681 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4741 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4801 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4861 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4921 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4981 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5041 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5101 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5161 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5221 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5281 tgacgagttc ttctgagcgg gactctgggg ttcgcatcga taaaataaaa gattttattt
     5341 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     5401 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
     5461 aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     5521 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata
     5581 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     5641 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     5701 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     5761 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
     5821 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     5881 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     5941 gggggtcttt cacaggccag tggggccgca cttttcgggg aaatgtgcgc ggaaccccta
     6001 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6061 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6121 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6181 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6241 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6301 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6361 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6421 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6481 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6541 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6601 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6661 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6721 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6781 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6841 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6901 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6961 accaagttta ctcatatata ctttagattg atttgcggcc gcgaaaagga caagcagcga
     7021 aaattcacgc ccccttggga ggtggcggca tatgcaaagg atagcactcc cactctacta
     7081 ctgggtatca tatgctgact gtatatgcat gaggatagca tatgctaccc ggatacagat
     7141 taggatagca tatactaccc agatatagat taggatagca tatgctaccc agatatagat
     7201 taggatagcc tatgctaccc agatataaat taggatagca tatactaccc agatatagat
     7261 taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat
     7321 taggatagca tatgctatcc agatatttgg gtagtatatg ctacccatgg caacattagc
     7381 ccaccgtgct ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc ttggcgctca
     7441 ggcgcaagtg tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta tcttggcccg
     7501 cccacctact tatgcaggta ttccccgggg tgccattagt ggttttgtgg gcaagtggtt
     7561 tgaccgcagt ggttagcggg gttacaatca gccaagttat tacaccctta ttttacagtc
     7621 caaaaccgca gggcggcgtg tgggggctga cgcgtgcccc cactccacaa tttcaaaaaa
     7681 aagagtggcc acttgtcttt gtttatgggc cccattggcg tggagccccg tttaattttc
     7741 gggggtgtta gagacaacca gtggagtccg ctgctgtcgg cgtccactct ctttcccctt
     7801 gttacaaata gagtgtaaca acatggttca cctgtcttgg tccctgcctg ggacacatct
     7861 taataacccc agtatcatat tgcactagga ttatgtgttg cccatagcca taaattcgtg
     7921 tgagatggac atccagtctt tacggcttgt ccccacccca tggatttcta ttgttaaaga
     7981 tattcagaat gtttcattcc tacactagta tttattgccc aaggggtttg tgagggttat
     8041 attggtgtca tagcacaatg ccaccactga accccccgtc caaattttat tctgggggcg
     8101 tcacctgaaa ccttgttttc gagcacctca catacacctt actgttcaca actcagcagt
     8161 tattctatta gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg agagttcact
     8221 gcccgctcct tgatcttcag ccactgccct tgtgactaaa atggttcact accctcgtgg
     8281 aatcctgacc ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat atcgctgttc
     8341 cttaggaccc ttttactaac cctaattcga tagcatatgc ttcccgttgg gtaacatatg
     8401 ctattgaatt agggttagtc tggatagtat atactactac ccgggaagca tatgctaccc
     8461 gtttagggtt aacaaggggg ccttataaac actattgcta atgccctctt gagggtccgc
     8521 ttatcggtag ctacacaggc ccctctgatt gacgttggtg tagcctcccg tagtcttcct
     8581 gggcccctgg gaggtacatg tcggccgcaa aaggatctag gtgaagatcc tttttgataa
     8641 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8701 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8761 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8821 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8881 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8941 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9001 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9061 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9121 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9181 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9241 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9301 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9361 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     9421 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     9481 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     9541 caactatggt tggcgcgttg taaacgttaa tattttgtta aaattcgcgt taaatttttg
     9601 ttaaatcagc tcatttttta accaatagac cgaaatcggc aaaatccctt ataaatcaaa
     9661 agaatagccc gagatagagt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     9721 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     9781 tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     9841 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     9901 ggaagggaag aaagcgaaag gagcgggcgc taaggcgctg gcaagtgtag cggtcacgct
     9961 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta caggg
//
