LOCUS       sequence-456535-       10717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10717)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 10717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag HK2
FEATURES             Location/Qualifiers
     source          1..10717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        149..528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        529..732
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     683..703
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    953..1152
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1353..1769
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1699..1721
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1737..1753
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1787..1796
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1793..1834
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1859..1882
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1889..1913
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             4369..4377
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(4665..4689)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4748..5209
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4804..4821)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5031..5050
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5214..6008
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5268..5287)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5878..5897
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6195..6664
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        6700..6804
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6805..6873,6874..7665)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9431..10019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9920..9939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10173..10190
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10294..10717,1..4))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10404..10425)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10616..10635
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgcgccgatc ttcaatattg gccattagcc atattattca ttggttatat agcataaatc
       61 aatattggct attggccatt gcatacgttg tatctatatc ataatatgta catttatatt
      121 ggctcatgtc caatatgacc gccatgttgg cattgattat tgactagtta ttaatagtaa
      181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      541 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      661 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      721 ataagcagag ctggtttagt gaaccgcgcc agtcctccga ttgactgagt cgcccgggta
      781 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
      841 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg
      901 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gcaagctggc
      961 cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc
     1021 gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt
     1081 tctgaacacc cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg
     1141 gcccgacctg aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg
     1201 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga
     1261 accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc
     1321 tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt
     1381 tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca
     1441 agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc
     1501 cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac
     1561 ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt
     1621 ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc
     1681 catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt
     1741 atccagccct cactccttct ctaggcgccg gccggatcca gaattcgcca ccatggggtc
     1801 ttcaaaatct aaaccaaagg accccagcca gcgccggcgc aggatccgag gttaccttga
     1861 ctacaaagac gatgacgaca agcaattgac aagtttgtac aaaaaagttg gcatgattgc
     1921 ctcgcatctg cttgcctact tcttcacgga gctcaaccat gaccaagtgc agaaggttga
     1981 ccagtatctc taccacatgc gcctctctga tgagaccctc ttggagatct ctaagcggtt
     2041 ccgcaaggag atggagaaag ggcttggagc caccactcac cctactgcag cagtgaagat
     2101 gctgcccacc tttgtgaggt ccactccaga tgggacagaa cacggagagt tcctggctct
     2161 ggatcttgga gggaccaact tccgtgtgct ttgggtgaaa gtaacggaca atgggctcca
     2221 gaaggtggag atggagaatc agatctatgc catccctgag gacatcatgc gaggcagtgg
     2281 cacccagctg tttgaccaca ttgccgaatg cctggctaac ttcatggata agctacaaat
     2341 caaagacaag aagctcccac tgggttttac cttctcgttc ccctgccacc agactaaact
     2401 agacgagagt ttcctggtct catggaccaa gggattcaag tccagtggag tggaaggcag
     2461 agacgttgtg gctctgatcc ggaaggccat ccagaggaga ggggactttg atatcgacat
     2521 tgtggctgtg gtgaatgaca cagttgggac catgatgacc tgtggttatg atgaccacaa
     2581 ctgtgagatt ggtctcattg tgggcacggg cagcaacgcc tgctacatgg aagagatgcg
     2641 ccacatcgac atggtggaag gcgatgaggg gcggatgtgt atcaatatgg agtggggggc
     2701 cttcggggac gatggctcgc tcaacgacat tcgcactgag tttgaccagg agattgacat
     2761 gggctcactg aacccgggaa agcaactgtt tgagaagatg atcagtggga tgtacatggg
     2821 ggagctggtg aggcttatcc tggtgaagat ggccaaggag gagctgctct ttggggggaa
     2881 gctcagccca gagcttctca acaccggtcg ctttgagacc aaagacatct cagacattga
     2941 aggggagaag gatggcatcc ggaaggcccg tgaggtcctg atgcggttgg gcctggaccc
     3001 gactcaggag gactgcgtgg ccactcaccg gatctgccag atcgtgtcca cacgctccgc
     3061 cagcctgtgc gcagccaccc tggccgccgt gctgcagcgc atcaaggaga acaaaggcga
     3121 ggagcggctg cgctctacta ttggggtcga cggttccgtc tacaagaaac acccccattt
     3181 tgccaagcgt ctacataaga ccgtgcggcg gctggtgccc ggctgcgatg tccgcttcct
     3241 ccgctccgag gatggcagtg gcaaaggtgc agccatggtg acagcagtgg cttaccggct
     3301 ggccgatcaa caccgtgccc gccagaagac attagagcat ctgcagctga gccatgacca
     3361 gctgctggag gtcaagagga ggatgaaggt agaaatggag cgaggtctga gcaaggagac
     3421 tcatgccagt gcccccgtca agatgctgcc cacctacgtg tgtgctaccc cggacggcac
     3481 agagaaaggg gacttcttgg ccttggacct tggaggaaca aatttccggg tcctgctggt
     3541 ccgtgttcgg aatgggaagt ggggtggagt ggagatgcac aacaagatct acgccatccc
     3601 gcaggaggtc atgcacggca ccggggacga gctctttgac cacattgtcc agtgcatcgc
     3661 ggacttcctc gagtacatgg gcatgaaggg cgtgtccctg cctctgggtt ttaccttctc
     3721 cttcccctgc cagcagaaca gcctggacga gagcatcctc ctcaagtgga caaaaggctt
     3781 caaggcatct ggctgcgagg gcgaggacgt ggtgaccctg ctgaaggaag cgatccaccg
     3841 gcgagaggag tttgacctgg atgtggttgc tgtggtgaac gacacagtcg gaactatgat
     3901 gacctgtggc tttgaagacc ctcactgtga agttggcctc attgttggca cgggcagcaa
     3961 tgcctgctac atggaggaga tgcgcaacgt ggaactggtg gaaggagaag aggggcggat
     4021 gtgtgtgaac atggaatggg gggccttcgg ggacaatgga tgcctagatg acttccgcac
     4081 agaatttgat gtggctgtgg atgagctttc actcaacccc ggcaagcaga ggttcgagaa
     4141 aatgatcagt ggaatgtacc tgggtgagat tgtccgtaac attctcatcg atttcaccaa
     4201 gcgtggactg ctcttccgag gccgcatctc agagcggctc aagacaaggg gcatctttga
     4261 aaccaagttc ttgtctcaga ttgagagtga ctgcctggcc ctgctgcaag tccgagccat
     4321 cctgcaacac ttagggcttg agagcacctg tgacgacagc atcattgtta aggaggtgtg
     4381 cactgtggtg gcccggcggg cagcccagct ctgtggcgca ggcatggccg ctgtggtgga
     4441 caggatacga gaaaaccgtg ggctggacgc tctcaaagtg acagtgggtg tggatgggac
     4501 cctctacaag ctacatcctc actttgccaa agtcatgcat gagacagtga aggacctggc
     4561 tccgaaatgt gatgtgtctt tcctgcagtc agaggatggc agcgggaagg gggcggcgct
     4621 catcactgct gtggcctgcc gcatccgtga ggctggacag cgatacccaa ctttcttgta
     4681 caaagtggtt tgatctagtc gaccaattcc ggttattttc caccatattg ccgtcttttg
     4741 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     4801 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     4861 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     4921 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     4981 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     5041 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     5101 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg
     5161 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat accatgattg
     5221 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     5281 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     5341 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     5401 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     5461 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     5521 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     5581 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     5641 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     5701 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     5761 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     5821 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     5881 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     5941 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     6001 tcttctgagc gggactctgg ggttcgcatc gataaaataa aagattttat ttagtctcca
     6061 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     6121 attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg
     6181 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     6241 cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     6301 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc
     6361 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     6421 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     6481 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
     6541 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     6601 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     6661 ttcacaggcc agtggggccg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6721 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6781 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6841 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6901 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6961 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     7021 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     7081 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     7141 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     7201 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     7261 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     7321 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     7381 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     7441 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7501 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7561 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7621 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7681 tactcatata tactttagat tgatttgcgg ccgcgaaaag gacaagcagc gaaaattcac
     7741 gcccccttgg gaggtggcgg catatgcaaa ggatagcact cccactctac tactgggtat
     7801 catatgctga ctgtatatgc atgaggatag catatgctac ccggatacag attaggatag
     7861 catatactac ccagatatag attaggatag catatgctac ccagatatag attaggatag
     7921 cctatgctac ccagatataa attaggatag catatactac ccagatatag attaggatag
     7981 catatgctac ccagatatag attaggatag cctatgctac ccagatatag attaggatag
     8041 catatgctat ccagatattt gggtagtata tgctacccat ggcaacatta gcccaccgtg
     8101 ctctcagcga cctcgtgaat atgaggacca acaaccctgt gcttggcgct caggcgcaag
     8161 tgtgtgtaat ttgtcctcca gatcgcagca atcgcgcccc tatcttggcc cgcccaccta
     8221 cttatgcagg tattccccgg ggtgccatta gtggttttgt gggcaagtgg tttgaccgca
     8281 gtggttagcg gggttacaat cagccaagtt attacaccct tattttacag tccaaaaccg
     8341 cagggcggcg tgtgggggct gacgcgtgcc cccactccac aatttcaaaa aaaagagtgg
     8401 ccacttgtct ttgtttatgg gccccattgg cgtggagccc cgtttaattt tcgggggtgt
     8461 tagagacaac cagtggagtc cgctgctgtc ggcgtccact ctctttcccc ttgttacaaa
     8521 tagagtgtaa caacatggtt cacctgtctt ggtccctgcc tgggacacat cttaataacc
     8581 ccagtatcat attgcactag gattatgtgt tgcccatagc cataaattcg tgtgagatgg
     8641 acatccagtc tttacggctt gtccccaccc catggatttc tattgttaaa gatattcaga
     8701 atgtttcatt cctacactag tatttattgc ccaaggggtt tgtgagggtt atattggtgt
     8761 catagcacaa tgccaccact gaaccccccg tccaaatttt attctggggg cgtcacctga
     8821 aaccttgttt tcgagcacct cacatacacc ttactgttca caactcagca gttattctat
     8881 tagctaaacg aaggagaatg aagaagcagg cgaagattca ggagagttca ctgcccgctc
     8941 cttgatcttc agccactgcc cttgtgacta aaatggttca ctaccctcgt ggaatcctga
     9001 ccccatgtaa ataaaaccgt gacagctcat ggggtgggag atatcgctgt tccttaggac
     9061 ccttttacta accctaattc gatagcatat gcttcccgtt gggtaacata tgctattgaa
     9121 ttagggttag tctggatagt atatactact acccgggaag catatgctac ccgtttaggg
     9181 ttaacaaggg ggccttataa acactattgc taatgccctc ttgagggtcc gcttatcggt
     9241 agctacacag gcccctctga ttgacgttgg tgtagcctcc cgtagtcttc ctgggcccct
     9301 gggaggtaca tgtcggccgc aaaaggatct aggtgaagat cctttttgat aatctcatga
     9361 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9421 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9481 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9541 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9601 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9661 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9721 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9781 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9841 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9901 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9961 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10021 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10081 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10141 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10201 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
    10261 gttggcgcgt tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
    10321 gctcattttt taaccaatag accgaaatcg gcaaaatccc ttataaatca aaagaatagc
    10381 ccgagataga gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
    10441 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
    10501 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
    10561 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
    10621 agaaagcgaa aggagcgggc gctaaggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
    10681 ccaccacacc cgccgcgctt aatgcgccgc tacaggg
//
