LOCUS       sequence-456548-        5562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Burkel BM, von Dassow G, Bement WM
  TITLE     Versatile fluorescent probes for actin filaments based on the 
            actin-binding domain of utrophin.
  JOURNAL   Cell Motil Cytoskeleton. 2007 Nov . 64(11):822-32.
  PUBMED    17685442
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Motil 
            Cytoskeleton. 2007 Nov . 64(11):822-32."
COMMENT     Sequence Label: mRFP-UtrCH
FEATURES             Location/Qualifiers
     source          1..5562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             88..762
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             88..762
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(218..236)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     486..505
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     680..703
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1614..1748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1638..1657)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1692..1711
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1861..1881)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1861..1879)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1900..1916)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1900..1916)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1913..1935)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1924..1940
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1948..1954,1955..1972,1973..1978))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1993..2014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2131..2148)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2302..2890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2382..2401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3061..3852,3853..3921))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3684..3703
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3922..4026)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4052..4507)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4189..4210)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4401..4420
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4578..5562
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccaccatg gcctcctccg aggacgtcat caaggagttc
      121 atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      181 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
      241 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tccagtacgg ctccaaggcc
      301 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      361 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      421 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      481 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcggatgtac
      541 cccgaggacg gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa ggacggcggc
      601 cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca gctgcccggc
      661 gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta caccatcgtg
      721 gaacagtacg agcgcgccga gggccgccac tccaccggcg cctccggaac catggccaag
      781 tatggagaac atgaagccag tcctgacaat gggcagaacg aattcagtga tatcattaag
      841 tccagatctg atgaacacaa tgacgtacag aagaaaacct ttaccaaatg gataaatgct
      901 cgattttcaa agagtgggaa accacccatc aatgatatgt tcacagacct caaagatgga
      961 aggaagctat tggatcttct agaaggcctc acaggaacat cactgccaaa ggaacgtggt
     1021 tccacaaggg tacatgcctt aaataacgtc aacagagtgc tgcaggtttt acatcagaac
     1081 aatgtggaat tagtgaatat agggggaacc gacattgtgg atggaaatca caaactgact
     1141 ttggggttac tttggagcat cattttgcac tggcaggtga aagatgtcat gaaggatgtc
     1201 atgtcggacc tgcagcagac gaacagtgag aagatcctgc tcagctgggt gcgtcagacc
     1261 accaggccct acagccaagt caacgtcctc aacttcacca ccagctggac agatggactc
     1321 gcctttaatg ctgtcctcca ccgacataaa cctgatctct tcagctggga taaagttgtc
     1381 aaaatgtcac caattgagag acttgaacat gccttcagca aggctcaaac ttatttggga
     1441 attgaaaagc tgttagatcc tgaagatgtt gccgttcggc ttcctgacaa gaaatccata
     1501 attatgtatt taacatcttt gtttgaggtg ctacctcagc aagtcaccat agactaactc
     1561 gagctcaagc ttcgaattct gcagtctaga actatagtga gtcgtattac gtagatccag
     1621 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1681 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1741 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1801 aggtttttta attcgcggcc gcggcgccaa tgcattgggc ccggtaccca gcttttgttc
     1861 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1921 aaattgttat ccgctcacaa ttccacacaa catacgagcc gggagcataa agtgtaaagc
     1981 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     2041 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     2101 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2161 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2221 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2281 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2341 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2401 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2461 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2521 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2581 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2641 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2701 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2761 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2821 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2881 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2941 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3001 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3061 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3121 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3181 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3241 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3301 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3361 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3421 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3481 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3541 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3601 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3661 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3721 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3781 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3841 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3901 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3961 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4021 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     4081 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     4141 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     4201 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     4261 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     4321 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     4381 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     4441 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     4501 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     4561 tcttcgctat tacgccagtc gaccgccaat tcaatatggc gtatatggac tcatgccaat
     4621 tcaatatggt ggatctggac ctgtgccaat tcaatatggc gtatatggac tcgtgccaat
     4681 tcaatatggt ggatctggac cccagccaat tcaatatggc ggacttggca ccatgccaat
     4741 tcaatatggc ggacctggca ctgtgccaac tggggagggg tctacttggc acggtgccaa
     4801 gtttgaggag gggtcttggc cctgtgccaa gtccgccata ttgaattggc atggtgccaa
     4861 taatggcggc catattggct atatgccagg atcaatatat aggcaatatc caatatggcc
     4921 ctatgccaat atggctattg gccaggttca atactatgta ttggccctat gccatatagt
     4981 attccatata tgggttttcc tattgacgta gatagcccct cccaatgggc ggtcccatat
     5041 accatatatg gggcttccta ataccgccca tagccactcc cccattgacg tcaatggtct
     5101 ctatatatgg tctttcctat tgacgtcata tgggcggtcc tattgacgta tatggcgcct
     5161 cccccattga cgtcaattac ggtaaatggc ccgcctggct caatgcccat tgacgtcaat
     5221 aggaccaccc accattgacg tcaatgggat ggctcattgc ccattcatat ccgttctcac
     5281 gccccctatt gacgtcaatg acggtaaatg gcccacttgg cagtacatca atatctatta
     5341 atagtaactt ggcaagtaca ttactattgg aagtacgcca gggtacattg gcagtactcc
     5401 cattgacgtc aatggcggta aatggcccgc gatggctgcc aagtacatcc ccattgacgt
     5461 caatggggag gggcaatgac gcaaatgggc gttccattga cgtaaatggg cggtaggcgt
     5521 gcctaatggg aggtctatat aagcaatgct cgtttaggga ac
//
