LOCUS       sequence-456580-        5963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Pfleger CM, Lee E, Kirschner MW
  TITLE     Substrate recognition by the Cdc20 and Cdh1 components of the 
            anaphase-promoting complex.
  JOURNAL   Genes Dev. 2001 Sep 15. 15(18):2396-407.
  PUBMED    11562349
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2001 Sep 15. 15(18):2396-407."
COMMENT     Sequence Label: MT-cdh1
FEATURES             Location/Qualifiers
     source          1..5963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             103..132
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             142..171
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             181..210
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             220..249
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             269..298
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             308..337
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             347..376
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             386..415
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             449..478
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(1070..1081)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2015..2149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2039..2058)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2093..2112
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2262..2282)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2262..2280)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2301..2317)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2301..2317)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2314..2336)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2325..2341
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2349..2355,2356..2373,2374..2379))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2394..2415
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2532..2549)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2703..3291)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2783..2802)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3462..4253,4254..4322))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4085..4104
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4323..4427)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4453..4908)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4590..4611)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4802..4821
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4979..5963
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tggagcaaaa gctcatttct
      121 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
      181 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
      241 gaggacttga atgaaatgga gtgaaatgga gcaaaagctc atttctgaag aggacttgaa
      301 tgaaatggag caaaagctca tttctgaaga ggacttgaat gaaatggagc aaaagctcat
      361 ttctgaagag gacttgaatg aaatggagca aaagctcatt tctgaagagg acttgaatga
      421 aatggagagc ttgggcgacc tcaccatgga gcaaaagctc atttctgaag aggacttgaa
      481 ttcgatggac caggactatg agcggcgcct gcttcgccag atcgtcatcc agaatgagaa
      541 cacgatgcca cgcgtcacag agatgcggcg gaccctgacg cctgccagct ccccagtgtc
      601 ctcgcccagc aagcacggag accgcttcat cccctccaga gccggagcca actggagcgt
      661 gaacttccac aggattaacg agaatgagaa gtctcccagt cagaaccgga aagccaagga
      721 cgccacctca gacaacggca aagacggcct ggcctactct gccctgctca agaatgagct
      781 gctgggtgcc ggcatcgaga aggtgcagga cccgcagact gaggaccgca gactgcagcc
      841 ctccacgcct gagaagaagg gtctgttcac gtattccctt agcaccaagc gctccagccc
      901 cgatgacggc aacgatgtgt ctccctactc cctgtctccc gtcagcaaca agagccagaa
      961 gctgctccgg tccccccgga aacccacccg caagatctcc aagatcccct tcaaggtgct
     1021 ggacgcgccc gagctgcagg acgacttcta cctcaatctg gtggactggt cgtccctcaa
     1081 tgtgctcagc gtggggctag gcacctgcgt gtacctgtgg agtgcctgta ccagccaggt
     1141 gacgcggctc tgtgacctct cagtggaagg ggactcagtg acctccgtgg gctggtctga
     1201 gcgggggaac ctggtggcgg tgggcacaca caagggcttc gtgcagatct gggacgcagc
     1261 cgcagggaag aagctgtcca tgttggaggg ccacacggca cgcgtcgggg cgctggcctg
     1321 gaatgctgag cagctgtcgt ccgggagccg cgaccgcatg atcctgcaga gggacatccg
     1381 caccccgcca ctgcagtcgg agcggcggct gcagggccac cggcaggagg tgtgcgggct
     1441 caagtggtcc acagaccacc agctcctcgc ctcggggggc aacgacaaca agctgctggt
     1501 ctggaatcac tcgagcctga gccccgtgca gcagtacacg gagcacctgg cggccgtgaa
     1561 ggccatcgcc tggtccccac atcagcacgg gctgctggcc tcggggggcg gcacagctga
     1621 ccgctgtatc cgcttctgga acacgctgac aggacaacca ctgcagtgta tcgacacggg
     1681 ctcccaagtg tgcaatctgg cctggtccaa gcacgccaac gagctggtga gcacgcacgg
     1741 ctactcacag aaccagatcc ttgtctggaa gtacccctcc ctgacccagg tggccaagct
     1801 gaccgggcac tcctaccgcg tgctgtacct ggcaatgtcc cctgatgggg aggccatcgt
     1861 cactggtgct ggagacgaga ccctgaggtt ctggaacgtc tttagcaaaa cccgttcgac
     1921 aaaggagtct gtgtctgtgc tcaacctctt caccaggatc cggtaatcta gagccctctc
     1981 gagcctctag aactatagtg agtcgtatta cgtagatcca gacatgataa gatacattga
     2041 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     2101 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     2161 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc
     2221 cgcggcgcca atgcattggg cccggtaccc agcttttgtt ccctttagtg agggttaatt
     2281 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     2341 attccacaca acatacgagc cgggagcata aagtgtaaag cctggggtgc ctaatgagtg
     2401 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     2461 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     2521 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2581 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2641 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2701 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2761 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2821 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2881 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2941 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3001 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3061 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3121 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3181 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3241 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3301 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3361 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3421 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3481 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3541 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3601 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3661 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3721 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3781 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3841 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3901 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3961 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4021 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4081 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4141 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4201 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4261 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4321 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4381 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4441 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     4501 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     4561 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     4621 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     4681 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     4741 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     4801 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     4861 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
     4921 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt
     4981 cgaccgccaa ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg tggatctgga
     5041 cctgtgccaa ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga
     5101 ccccagccaa ttcaatatgg cggacttggc accatgccaa ttcaatatgg cggacctggc
     5161 actgtgccaa ctggggaggg gtctacttgg cacggtgcca agtttgagga ggggtcttgg
     5221 ccctgtgcca agtccgccat attgaattgg catggtgcca ataatggcgg ccatattggc
     5281 tatatgccag gatcaatata taggcaatat ccaatatggc cctatgccaa tatggctatt
     5341 ggccaggttc aatactatgt attggcccta tgccatatag tattccatat atgggttttc
     5401 ctattgacgt agatagcccc tcccaatggg cggtcccata taccatatat ggggcttcct
     5461 aataccgccc atagccactc ccccattgac gtcaatggtc tctatatatg gtctttccta
     5521 ttgacgtcat atgggcggtc ctattgacgt atatggcgcc tcccccattg acgtcaatta
     5581 cggtaaatgg cccgcctggc tcaatgccca ttgacgtcaa taggaccacc caccattgac
     5641 gtcaatggga tggctcattg cccattcata tccgttctca cgccccctat tgacgtcaat
     5701 gacggtaaat ggcccacttg gcagtacatc aatatctatt aatagtaact tggcaagtac
     5761 attactattg gaagtacgcc agggtacatt ggcagtactc ccattgacgt caatggcggt
     5821 aaatggcccg cgatggctgc caagtacatc cccattgacg tcaatgggga ggggcaatga
     5881 cgcaaatggg cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata
     5941 taagcaatgc tcgtttaggg aac
//
