LOCUS       sequence-456743-        6367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Sekiya S, Suzuki A
  TITLE     Direct conversion of mouse fibroblasts to hepatocyte-like cells by 
            defined factors.
  JOURNAL   Nature. 2011 Jun 29;475(7356):390-3. doi: 10.1038/nature10263.
  PUBMED    21716291
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-06-29- 29"; volume: "475"; issue: "7356"; 
            pages: "390-3"
COMMENT     Sequence Label: pGCDNsam-Hnf4a
FEATURES             Location/Qualifiers
     source          1..6367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(528..1319,1320..1388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1151..1170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1389..1493)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1561..1579
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             2151..2326
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    2390..2731
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             2798..3214
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3144..3166
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3182..3198
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     3483..3499
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             5082..5596
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5758..5774
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5782..5788,5789..5806,5807..5812))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5827..5848
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5965..5982)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(6136..6367,1..357))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6216..6235)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
       61 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      121 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      181 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      241 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      301 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      361 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
      421 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      481 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
      541 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
      601 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
      661 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
      721 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
      781 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
      841 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
      901 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
      961 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1021 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1081 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1141 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1201 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     1261 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     1321 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     1381 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1441 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     1501 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     1561 aataggcgta tcacgaggcc ctttcgtctt caagaattca taccagatca ccgaaaactg
     1621 tcctccaaat gtgtccccct cacactccca aattcgcggg cttctgcctc ttagaccact
     1681 ctaccctatt ccccacactc accggagcca aagccgcggc ccttccgttt ctttgctttt
     1741 gaaagacccc acccgtaggt ggcaagctag cttaagtaac gccactttgc aaggcatgga
     1801 aaaatacata actgagaata gaaaagttca gatcaaggtc aggaacaaag aaacagctga
     1861 ataccaaaca ggatatctgt ggtaagcggt tcctgccccg gctcagggcc aagaacagat
     1921 gagacagctg agtgatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     1981 ggggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagtgaatca
     2041 tcagatgttt ccagggtgcc ccaaggacct gaaaatgacc ctgtacctta tttgaactaa
     2101 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttccgc tctccgagct caataaaaga
     2161 gcccacaacc cctcactcgg cgcgccagtc ttccgataga ctgcgtcgcc cgggtacccg
     2221 tattcccaat aaagcctctt gctgtttgca tccgaatcgt ggactcgctg atccttggga
     2281 gggtctcctc agattgattg actgcccacc tcgggggtct ttcatttgga ggttccaccg
     2341 agatttggag acccctgccc agggaccacc gacccccccg ccgggaggta agctggccag
     2401 cggtcgtttc gtgtctgtct ctgtctttgt gcgtgtttgt gccggcatct aatgtttgcg
     2461 cctgcgtctg tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga
     2521 cgagttctga acacccggcc gcaaccctgg gagacgtccc agggactttg ggggccgttt
     2581 ttgtggcccg acctgaggaa gggagtcgat gtggaatccg accccgtcag gatatgtggt
     2641 tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt
     2701 ttggaaccga agccgcgcgt cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct
     2761 ctgtctgact gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt
     2821 aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga
     2881 tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg
     2941 atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt
     3001 ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt
     3061 ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct
     3121 tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc
     3181 cctttatcca gccctcactc cttctctagg cgccggaatt cgcggccgtg acaagagtta
     3241 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct
     3301 ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta cctcaccctt
     3361 accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta gaacctcgct
     3421 ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta gacggcatcg
     3481 cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga ccatcctcta
     3541 gactgacgcg gccgcgggaa ttcgattgta tgcgactctc taaaaccctt gccggcatgg
     3601 atatggccga ctacagcgct gccctggacc cagcctacac caccctggag tttgaaaatg
     3661 tgcaggtgtt gaccatgggc aatgacacgt ccccatctga aggtgccaac ctcaattcat
     3721 ccaacagcct gggcgtcagt gccctgtgcg ccatctgtgg cgaccgggcc accggcaaac
     3781 actacggagc ctcgagctgt gacggctgca aggggttctt caggaggagc gtgaggaaga
     3841 accacatgta ctcctgcagg tttagccgac aatgtgtggt agacaaagat aagaggaacc
     3901 agtgtcgtta ctgcaggctt aagaagtgct tccgggctgg catgaagaag gaagctgtcc
     3961 aaaatgagcg ggaccggatc agcacgcgga ggtcaagcta cgaggacagc agcctgccct
     4021 ccatcaacgc gctcctgcag gcagaggttc tgtcccagca gatcacctct cccatctctg
     4081 ggatcaatgg cgacattcgg gcaaagaaga ttgccaacat cacagacgtg tgtgagtcta
     4141 tgaaggagca gctgctggtc ctggtcgagt gggccaagta catcccggcc ttctgcgaac
     4201 tccttctgga tgaccaggtg gcgctgctca gggcccacgc cggtgagcat ctgctgcttg
     4261 gagccaccaa gaggtccatg gtgtttaagg acgtgctgct cctaggcaat gactacatcg
     4321 tccctcggca ctgtccagag ctagcggaga tgagccgtgt gtccatccgc atcctcgatg
     4381 agctggtcct gcccttccaa gagctgcaga ttgatgacaa tgaatatgcc tgcctcaaag
     4441 ccatcatctt ctttgatcca gatgccaagg ggctgagtga cccgggcaag atcaagcggc
     4501 tgcggtcaca ggtgcaagtg agcctggagg attacatcaa cgaccggcag tacgactctc
     4561 ggggccgctt tggagagctg ctgctgctgt tgcccacgct gcagagcatc acctggcaga
     4621 tgatcgaaca gatccagttc atcaagctct tcggcatggc caagattgac aacctgctgc
     4681 aggagatgct tctcggaggg tctgccagtg atgcacccca cacccaccac cccctgcacc
     4741 ctcacctgat gcaagaacac atgggcacca atgtcattgt tgctaacacg atgccctctc
     4801 acctcagcaa tggacagatg tgtgagtggc cccgacccag ggggcaggca gccactcccg
     4861 agactccaca gccatcacca ccaagtggct cgggatctga atcctacaag ctcctgccag
     4921 gagccatcac caccatcgtc aagcctccct ctgccattcc ccagccaacg atcaccaagc
     4981 aagaagccat ctaggcaatc actagtgaat tcgcggccgc tacgtagtcg acggatccga
     5041 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     5101 ggtttggcaa gctagcttaa gtaagcttat tttgcaaggc atggaaaata cataactgag
     5161 aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg
     5221 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     5281 cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     5341 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     5401 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt
     5461 cctccgatag actgcgtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     5521 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     5581 agcgggggtc tttcatgggt aacagtttct tgaagttgga gaacaacatt ctgagggtag
     5641 gagtcgaata ttaagtaatc ctgactcaat tagccactgt tttgaatcca catactccaa
     5701 tactcctgaa atagttcatt atggacagcg cagaaagagc tggggagaat tgtgaaattg
     5761 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     5821 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     5881 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     5941 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6001 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6061 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6121 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6181 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6241 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6301 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6361 gtaggtc
//
