LOCUS       sequence-457182-       11534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cytoplasmic-targeting Csm complex plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Xia C, Colognori D, Jiang XS, Xu K, Doudna JA
  TITLE     Single-molecule live-cell RNA imaging with CRISPR-Csm.
  JOURNAL   Nat Biotechnol. 2025 Feb 18. doi: 10.1038/s41587-024-02540-5.
  PUBMED    39966655
REFERENCE   2  (bases 1 to 11534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Feb 18. doi: 10.1038/s41587-024-02540-5."
COMMENT     Sequence Label: pCX420
FEATURES             Location/Qualifiers
     source          1..11534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             268..291
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2575..2622
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2653..2856
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2887..2896
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2896..2919
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    3307..3354
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        3385..3588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3619..3628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3628..3651
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4321..5031
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             4321..5031
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             5035..5751
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYKG"
     CDS             5035..5751
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYKG"
     polyA_signal    5752..5799
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5826..6029
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      6060..6069
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6069..6092
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    6999..7046
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        7077..7280
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      7311..7320
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7320..7343
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    8424..8471
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        8502..8705
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      8736..8745
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8745..8768
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    9507..9554
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(9694..9712)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9780..9884
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9885..9953,9954..10745)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10103..10122)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10916..11504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11405..11424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatctctag agccgccacc
      241 atgcaccatc accatcacca ttccggcgat tacaaagacg atgacgataa gcggggcatg
      301 aagaaagaaa agattgatct gttttacgga gccctgctgc acgacatcgg aaaggtcatc
      361 cagcgagcaa ccggagagcg gaagaaacac gcacttgtgg gcgccgactg gttcgacgag
      421 atcgccgaca accaagtcat ctcggatcag atccggtacc atatggccaa ctaccagtct
      481 gataagctcg gcaacgatca cctggcttac atcacctaca ttgccgacaa catcgcctcc
      541 ggtgtcgacc gccggcaatc caacgaagag tcagacgaag atacctccgc aaagatctgg
      601 gacacctaca cgaaccaggc cgacatcttt aacgtgttcg gagcgcagac cgataagcgg
      661 tacttcaagc ctaccgtgct gaatctcaag tcgaagccca acttcgcgtc cgccacttac
      721 gaacccttta gcaagggcga ttacgctgcc atcgccaccc ggattaagaa cgaactggcc
      781 gagttcgagt tcaaccaagt ccagattgac tccctgctca accttttcga ggctactctc
      841 tccttcgtgc cgtcaagcac caacactaag gaaatcgccg acatctccct ggccgaccat
      901 tcccgcttga ctgctgcctt cgctctggcg atctacgact acctggagga caagggtcgg
      961 cacaactaca aagaggacct gttcaccaaa gtgtcagcgt tctatgaaga agaagccttc
     1021 ctgctggcct ccttcgacct gtcgggaatc caggacttta tctacaacat taacatcgca
     1081 actaacggcg cggcgaagca gctgaaggcc cggagcctct acctggactt tatgtccgag
     1141 tacatcgccg atagcctgct ggacaagctg ggactgaaca gggctaacat gctttacgtc
     1201 ggcggcggac acgcctactt cgtcctggcc aacaccgaaa agactgtgga aaccctggtg
     1261 cagtttgaga aggatttcaa ccagttcctg ttggcaaact tccagacccg cctctatgtg
     1321 gcctttggct ggggttcctt cgcggccaag gacatcatgt ccgagctgaa tagccccgag
     1381 tcctaccgcc aagtgtacca aaaggcttcg cgcatgatct ccaaaaagaa aatctccaga
     1441 tacgactacc agacactgat gctcctgaat cgcggtggaa agtcctcaga gagagagtgc
     1501 gagatttgcc actccgtgga gaacctggtg tcctaccacg accagaaagt ctgtgacatt
     1561 tgccggggac tgtaccagtt ctcgaaagaa attgcccatg accacttcat cattaccgaa
     1621 aatgaggggc tgccgattgg accaaacgcg tgcttaaagg gcgtggcatt cgaaaagctg
     1681 tcccaagaag cgttcagccg ggtctacgtg aagaatgact ataaggccgg taccgtgaag
     1741 gctacgcatg tgttcgtggg ggattaccag tgcgacgaga tctacaacta cgccgccctg
     1801 agcaagaacg agaacggcct aggcatcaag agactggccg tggtccggct cgacgtggat
     1861 gacttgggcg ccgccttcat ggccggtttc agccagcagg gaaacggaca atactccact
     1921 ctgtcaagat cggccacatt ctcccggagc atgtcgctgt tcttcaaagt gtacattaac
     1981 cagttcgcct ccgacaagaa gctgagcatt atctacgcgg gcggcgatga cgtgttcgcc
     2041 attggatcgt ggcaggatat catcgcgttc actgtggaac ttcgcgaaaa cttcatcaag
     2101 tggaccaacg ggaagctcac cctctccgcg gggatagggt tgttcgccga caagactcct
     2161 attagcctga tggctcacca gaccggggaa ctggaagagg ccgccaaggg caacgaaaag
     2221 gactccatct cgctgttctc aagcgactac actttcaagt ttgataggtt catcactaac
     2281 gtgtacgacg acaaactgga acagattaga tacttcttca accatcaaga cgagagggga
     2341 aagaacttca tctataagct tattgagctt ttgaggaacc acgaccgcat gaatatggca
     2401 cgcctcgcct attacctcac tcgcctggaa gaactgaccc gggagactga cagggacaag
     2461 ttcaagacct tcaagaacct gttctactcc tggtacacca acaagaacga taaggaccgg
     2521 aaggaagccg agctcgcgct cctgctgtac atctacgaaa tcagaaagga ttaacggcaa
     2581 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcgcacacat tagctagccg
     2641 tcagcacaca ttgtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2701 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2761 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2821 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatctct
     2881 agagccgcca ccatggatta caaagacgat gacgataagc ggggcatgac catcctgacc
     2941 gacgagaact acgtggacat cgccgagaaa gccatcctga agctggaaag aaacaccaga
     3001 aatagaaaga accctgatgc cttcttcctg accacatcta agctgcggaa cctgctgagc
     3061 ctgacaagca ccctgttcga cgagagcaag gtgaaggaat acgacgccct gctggacaga
     3121 atcgcttatc tgagagtgca gttcgtgtac caggccggca gagagatcgc cgtgaaagat
     3181 ctgatcgaga aggcccagat cctggaagct ctgaaagaga tcaaggaccg ggaaaccctg
     3241 cagagattct gcagatacat ggaagccctg gtggcctact tcaagttcta cggcggcaag
     3301 gactgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcagttct
     3361 tttgccttac tttcaatgca tgcggtgatg cggttttggc agtacatcaa tgggcgtgga
     3421 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     3481 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     3541 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     3601 cgtcagatct ctagagccgc caccatggat tacaaagacg atgacgataa gcggggcatg
     3661 accttcgcca agatcaaatt cagcgcccag atccggctgg aaaccggcct gcacatcgga
     3721 ggatctgatg cctttgccgc tatcggcgcc atcgccagcc ctgtgatcaa ggaccccatc
     3781 accaacctgc ctatcatccc cggctctagc ctgaagggca agatgagaac actgctggcc
     3841 aaggtgtaca acgaaaaggt ggccgagaag cctagcgacg acagcgacat cctgagcaga
     3901 ctgttcggaa atagcaagga taagcggttc aagatgggca gactgatctt ccgggacgcc
     3961 ttcctgagca acgccgacga gctggattct ctgggcgtgc ggagctacac cgaggtgaag
     4021 ttcgagaaca ccatcgatag aatcaccgcc gaggccaatc ctagacagat cgagagagcc
     4081 attcggaact caacattcga cttcgagctg atctacgaga tcactgatga gaatgagaac
     4141 caggtcgagg aagatttcaa ggtgatcaga gacggcctga agctgctgga actggactac
     4201 ctgggcggaa gcggctccag aggctacggc aaagtggctt ttgagaacct gaaagccacc
     4261 acagtgttcg gcaactacga cgtgaaaacc ctgaacgagc tgctgaccgc cgaagtgggt
     4321 agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     4381 gttaatgggc acaaattttc tgtccgtgga gagggtgaag gtgatgctac aaacggaaaa
     4441 ctcaccctta aatttatttg cactactgga aaactacctg ttccgtggcc aacacttgtc
     4501 actactctga cctatggtgt tcaatgcttt tcccgttatc cggatcacat gaaacggcat
     4561 gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat atctttcaaa
     4621 gatgacggga cctacaagac gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     4681 cgtatcgagt taaagggtat tgattttaaa gaagatggaa acattcttgg acacaaactc
     4741 gagtacaact ttaactcaca caatgtatac atcacggcag acaaacaaaa gaatggaatc
     4801 aaagctaact tcaaaattcg ccacaacgtt gaagatggtt ccgttcaact agcagaccat
     4861 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     4921 tcgacacaat ctgtcctttc gaaagatccc aacgaaaagc gtgaccacat ggtccttctt
     4981 gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa aggttctaaa
     5041 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     5101 gggcacaaat tttctgtccg tggagagggt gaaggtgatg ctacaaacgg aaaactcacc
     5161 cttaaattta tttgcactac tggaaaacta cctgttccgt ggccaacact tgtcactact
     5221 ctgacctatg gtgttcaatg cttttcccgt tatccggatc acatgaaacg gcatgacttt
     5281 ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt caaagatgac
     5341 gggacctaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt taatcgtatc
     5401 gagttaaagg gtattgattt taaagaagat ggaaacattc ttggacacaa actcgagtac
     5461 aactttaact cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagct
     5521 aacttcaaaa ttcgccacaa cgttgaagat ggttccgttc aactagcaga ccattatcaa
     5581 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtcgaca
     5641 caatctgtcc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct tcttgagttt
     5701 gtaactgctg ctgggattac acatggcatg gatgagctct acaaaggtta acggcaataa
     5761 aaagacagaa taaaacgcac ggtgttgggt cgtttgttct tgcgagaatg gactagtagc
     5821 aaactgtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     5881 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     5941 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     6001 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc tctagagccg
     6061 ccaccatgga ttacaaagac gatgacgata agcggggcat gacttacaag ctctacatta
     6121 tgacctttca aaacgcccac ttcggttccg gcactctgga ctcatcgaag ctgaccttct
     6181 ccgcggatag aatcttctcg gcactcgtgc tcgaggctct gaagatggga aagctcgacg
     6241 ccttcttggc cgaggccaac caggataagt tcactctgac cgacgcgttc ccattccaat
     6301 tcggtccttt cctgccgaaa ccgattggtt accccaagca cgaccagatc gaccagtctg
     6361 tggacgtgaa ggaagtccgc cgccaagcga agctgtccaa aaagctccag ttcctggctc
     6421 tggaaaacgt cgacgactac ctgaacggag agctgtttga gaatgaggaa cacgccgtga
     6481 tcgacacagt gaccaagaac cagccccata aagatgataa tctgtaccaa gtggccacca
     6541 ctcggttctc gaacgacacc tccctttacg tgatcgccaa cgaatccgat ctgctgaacg
     6601 aactgatgag cagccttcag tactccgggc tgggcggcaa aaggtcctca ggattcggca
     6661 gatttgagct ggacatccag aacattccct tggaactgtc cgaccggctg acgaagaacc
     6721 acagcgacaa ggtcatgtca cttaccaccg ccctcccggt ggacgctgat ctcgaggaag
     6781 cgatggaaga tggccattac ctgttgacca agtcgtccgg attcgcattc tcccacgcca
     6841 ccaacgaaaa ctatcggaag caggacctgt acaagttcgc ctccgggagc accttcagca
     6901 agactttcga gggacagatc gtggacgtgc gccctctcga tttccctcac gccgtgctga
     6961 actacgccaa gccgctgttc tttaagctcg aagtctaacg gcaataaaaa gacagaataa
     7021 aacgcacggt gttgggtcgt ttgttcgcac attcaaaaac aggcaattgg acaagcgtga
     7081 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     7141 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     7201 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     7261 aggtctatat aagcagagct cgtttagtga accgtcagat ctctagagcc gccaccatgg
     7321 attacaaaga cgatgacgat aagcggggca tgaaaaatga ctaccggacc ttcaagctga
     7381 gcctgctgac cctggctcct atccacatcg gcaacggcga gaagtacacc agcagagaat
     7441 tcatctacga gaacaagaag ttctacttcc ccgacatggg caagttctac aacaagatgg
     7501 tggaaaagag actggccgag aagttcgagg ccttcctgat ccagaccaga cccaacgcca
     7561 gaaacaaccg gctgatttct tttctgaacg acaacagaat cgccgaaaga tcttttggcg
     7621 gctacagcat cagtgaaacc ggcctggaat ctgataagaa ccctaacagc gccggagcta
     7681 tcaacgaggt gaacaaattc atccgggacg ccttcggaaa tccttacatc ccaggcagca
     7741 gcctgaaggg cgccatccgc accatcctga tgaacaccac acctaagtgg aacaacgaga
     7801 acgccgtgaa cgacttcggc agattcccaa aggaaaacaa gaacctgatc ccttggggac
     7861 ctaagaaagg caaggaatac gacgacctgt tcaacgccat cagagtgtcc gacagcaagc
     7921 ccttcgacaa caaaagcctg atcctcgtgc agaagtggga ctacagcgcc aaaaccaaca
     7981 aggccaagcc tctgcctctg tacagagagt ctatcagccc tctgaccaag atcgagttcg
     8041 agataacaac aaccactgat gaggccggca gactgatcga ggaactggga aagcgggccc
     8101 aggcctttta taaggactac aaggcctttt tcctgtctga attccctgat gataagatcc
     8161 aggctaatct gcaatacccc atctacctgg gcgccggcag cggcgcttgg acaaagaccc
     8221 tgtttaagca ggccgacggc atcctgcagc ggagatactc cagaatgaaa accaagatgg
     8281 tcaagaaggg cgtgctgaag ctgacaaagg cccctctgaa aacagtgaag atccccagcg
     8341 gcaaccacag cctggtgaag aatcacgaga gcttctacga gatgggcaaa gccaacttca
     8401 tgatcaagga aatcgacaag tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     8461 gtcgtttgtt catgtgtgcc ggccggagaa taaaagtcta agtgatgcgg ttttggcagt
     8521 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     8581 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     8641 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     8701 gagctcgttt agtgaaccgt cagatctcta gagccgccac catggattac aaagacgatg
     8761 acgataagcg gggcatgaaa aagctcgtgt tcacctttaa gcggatcgac caccctgctc
     8821 aggacctggc cgtgaaattc cacggcttcc tgatggaaca gctggatagc gactacgtgg
     8881 actacctgca ccagcagcag accaacccct acgccacaaa ggtgatccag ggcaaagaga
     8941 acacccagtg ggtcgtgcat ctgctgacag acgacatcga ggacaaggtg ttcatgaccc
     9001 tgctgcagat caaggaagtg tccctgaacg acctgcctaa gttgtctgtg gaaaaggtgg
     9061 aaatccagga gctgggcgct gataagctgc tcgagatctt caacagcgag gaaaaccaga
     9121 cctacttcag catcatcttc gagacaccta caggctttaa aagccagggc agctacgtga
     9181 tcttccccag catgcggctg atctttcaga gcctgatgca gaagtacggc agactggtgg
     9241 aaaaccagcc tgagatcgag gaagataccc tggactacct gagcgagcac agcaccatca
     9301 ccaattacag actggaaaca agctacttca gagtgcatag acagagaatc cccgccttcc
     9361 ggggcaagct gaccttcaag gtgcagggag cccagacact gaaggcctac gtgaagatgc
     9421 tgctgacctt cggcgagtac agcggcctgg gcatgaaaac cagcctggga atgggcggca
     9481 tcaagctgga agaaagaaag gactgacggc aataaaaaga cagaataaaa cgcacggtgt
     9541 tgggtcgttt gttcgtgcga tagagggatc ccgcattgaa ttatgagcag attgtactga
     9601 gagtgcaccg gtccgtcttc gatgaagcga ttcagaagac ttacatgtgt cagaggtttt
     9661 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     9721 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     9781 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9841 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     9901 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9961 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    10021 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    10081 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    10141 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    10201 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10261 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    10321 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10381 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    10441 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10501 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    10561 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    10621 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    10681 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    10741 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    10801 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    10861 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    10921 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    10981 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    11041 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    11101 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    11161 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    11221 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    11281 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    11341 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    11401 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    11461 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    11521 cctttttctt aagc
//
