LOCUS       sequence-458045-        5283 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5283)
  TITLE     Hurley Lab Plasmids 2025
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCAG/MBP-VPS15(R103K)-TSF
FEATURES             Location/Qualifiers
     source          1..5283
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..1133
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MGIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1107..1130
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 gaattcctcg atcgacggta tcgatagcca ccatggggat cgaagaaggt aaactggtaa
       61 tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag aaattcgaga
      121 aagataccgg aattaaagtc accgttgagc atccggataa actggaagag aaattcccac
      181 aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac cgctttggtg
      241 gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc
      301 tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg
      361 ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg ccaaaaacct
      421 gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt
      481 tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt
      541 tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcga
      601 aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg
      661 attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc
      721 cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga
      781 ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg
      841 ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag
      901 gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg
      961 aagagttggc gaaagatcca cgtattgccg ccactatgga aaacgcccag aaaggtgaaa
     1021 tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact gcggtgatca
     1081 acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag actaattcga
     1141 gctcgaacaa caacaacaat aacaataaca acaacctcgg gatgggaaat cagctggccg
     1201 gcatcgctcc tagccagatt ctgtccgtgg aatcttactt cagtgacatc catgattttg
     1261 agtatgacaa atcactggga agcacccggt tctttaaggt ggcccgggct aaacacagag
     1321 aaggcctggt ggtggtgaag gtgttcgcta tccaggatcc cactctgcct ctgaccagtt
     1381 ataaacagga gctggaggaa ctgaagatta gactgaactc agcccagaat tgcctgccat
     1441 tccagaaagc tagcgagaag gcctccgaaa aagccgctat gctgtttaag cagtacgtgc
     1501 gcgacaacct gtatgatcgg atttccacaa gacccttcct gaacaatatc gagaagcggt
     1561 ggatcgcctt tcagattctg actgctgtgg accaggccca taaatctgga gtgagacacg
     1621 gcgatatcaa gactgagaac gtgatggtga ccagctggaa ttgggtgctg ctgacagact
     1681 tcgcttcctt taagccaact tacctgcccg aagacaaccc tgccgatttc aattatttct
     1741 ttgacactag ccggagaagg acctgctaca tcgctcctga gcggttcgtg gatggcggga
     1801 tgtttgccac cgagctggaa tacatgagag acccatcaac acccctggtg gatctgaaca
     1861 gcaatcagag gaggagagga gagctgaaga gggctatgga catcttttct gctgggtgtg
     1921 tgattgccga gctgttcacc gaaggagtgc ctctgtttga tctgagtcag ctgctggcct
     1981 ataggaacgg acacttcttt ccagagcagg tgctgaataa gatcgaggat catagcattc
     2041 gcgaactggt gacacagatg atccacaggg aaccagacaa acgcctggag gctgaagatt
     2101 acctgaagca gcagagaggc aacgccttcc cagagatctt ctacacattt ctgcagccct
     2161 atatggccca gttcgctaaa gagacttttc tgagcgccga cgaacggatc ctggtcatca
     2221 gaaaggatct gggaaacatc attcataatc tgtgcggcca cgacctgccc gaaaaagccg
     2281 agggcgaacc taaggagaat gggctggtca tcctggtgtc tgtgattaca agttgcctgc
     2341 agactctgaa gtattgtgat agcaaactgg ccgctctgga actgatcctg catctggctc
     2401 caaggctgtc cgtggagatc ctgctggaca gaattacccc ttacctgctg cacttctcca
     2461 acgattctgt gccacgggtg agagccgagg ctctgaggac actgactaaa gtgctggccc
     2521 tggtgaagga agtgcctcgc aacgacatca atatctaccc cgagtatatc ctgcctggca
     2581 ttgcccacct ggctcaggac gatgccacta ttgtgaggct ggcctatgct gagaatatcg
     2641 ccctgctggc tgaaaccgcc ctgcgctttc tggagctggt gcagctgaag aacctgaata
     2701 tggaaaacga ccccaacaat gaggaaatcg atgaggtgac ccatcctaac gggaattacg
     2761 acacagagct gcaggccctg cacgaaatgg tgcagcagaa agtggtgacc ctgctgtccg
     2821 atccagaaaa cattgtgaag cagactctga tggagaatgg gatcaccagg ctgtgtgtgt
     2881 tctttggacg ccagaaagct aacgacgtgc tgctgtcaca tatgatcaca ttcctgaacg
     2941 acaagaatga ttggcacctg cggggcgcct tctttgatag catcgtggga gtggccgctt
     3001 atgtgggctg gcagagctcc tctattctga agcctctgct gcagcagggg ctgtctgacg
     3061 ccgaggaatt tgtgatcgtg aaagctctgt atgccctgac ctgcatgtgt cagctgggac
     3121 tgctgcagaa gccacatgtg tacgagttcg ctagcgatat cgccccattt ctgtgccacc
     3181 ccaatctgtg gattcggtat ggggccgtgg gattcatcac tgtggtggct agacagatta
     3241 gcaccgccga cgtgtactgt aaactgatgc catacctgga tccctatatc acacagccca
     3301 tcattcagat tgaacggaaa ctggtgctgc tgtccgtgct gaaggagcct gtgtctagga
     3361 gtattttcga ctacgccctg cgcagcaagg atatcacttc cctgtttaga cacctgcata
     3421 tgaggcagaa gaaacgcaac ggatccctgc ctgactgccc acctccagag gatccagcca
     3481 tcgctcagct gctgaagaaa ctgctgtctc aggggatgac cgaggaagag gaagacaagc
     3541 tgctggccct gaaagatttc atgatgaagt ctaacaaggc caaagctaat atcgtggacc
     3601 agagtcacct gcatgatagt tcacagaagg gcgtgatcga cctggccgct ctgggcatta
     3661 cagggaggca ggtggatctg gtgaagacta aacaggagcc cgacgataaa agggcccgca
     3721 agcacgtgaa acaggacagc aacgtgaatg aggaatggaa gtcaatgttt ggaagcctgg
     3781 acccccctaa tatgcctcag gctctgccaa aaggcagcga tcaggaagtg atccagaccg
     3841 ggaagccacc aagatcagag agctccgccg gcatttgtgt gcccctgagc acatctagtc
     3901 aggtgcctga ggtgaccaca gtgcagaaca agaaacctgt gatcccagtg ctgtcaagca
     3961 ccattctgcc aagcacatac cagatcagga ttactacctg caaaaccgaa ctgcagcagc
     4021 tgatccagca gaagcgggag cagtgtaacg ctgaaagaat tgccaagcag atgatggaga
     4081 atgccgagtg ggaaagtaaa cctccaccac ctggatggag gccaaaggga ctgctggtgg
     4141 ctcacctgca tgaacacaaa agcgccgtga atcggatcag agtgtccgac gagcactctc
     4201 tgttcgccac atgctccaac gatggaactg tgaagatttg gaattctcag aagatggagg
     4261 gcaaaacaac taccacaagg tctatcctga cctatagtcg gattggaggc agagtgaaga
     4321 ccctgacatt ttgtcagggc agccattacc tggccatcgc ttccgacaac ggggctgtgc
     4381 agctgctggg aattgaagcc tcaaagctgc ccaaaagccc taagatccac cccctgcagt
     4441 ccaggattct ggaccagaag gaggatggct gcgtggtgga catgcaccat ttcaacagtg
     4501 gggcccagtc agtgctggcc tacgctacag tgaatgggtc tctggtggga tgggatctgc
     4561 gctcctctag taacgcttgg actctgaagc atgacctgaa atccggcctg atcacctctt
     4621 tcgccgtgga tattcaccag tgctggctgt gtatcggcac atcaagcggg actatggctt
     4681 gctgggacat gcgctttcag ctgccaatct cctctcattg tcacccaagc agggctagga
     4741 ttaggaggct gtctatgcac cctctgtatc agagttgggt catcgccgct gtgcagggga
     4801 acaatgaagt gtccatgtgg gacatggaga ccggagatcg gagattcaca ctgtgggcta
     4861 gttcagcccc acccctgtct gagctgcagc ctagtccaca ttcagtgcac gggatctact
     4921 gttcccctgc tgatggaaat ccaatcctgc tgaccgccgg ctctgacatg aaaattaggt
     4981 tttgggatct ggcttaccca gagcgcagtt atgtggtggc cggctcaacc agctccccct
     5041 cagtgagcta ctatcggaag atcattgagg gcacagaagt ggtgcaggaa atccagaaca
     5101 agcagaaagt gggaccaagt gacgatactc caaggagggg acctgagtca ctgccagtgg
     5161 gacaccatga catcattacc gatgtggcta cattccagac tacccagggc ttcatcgtga
     5221 ccgcctccag agacggcatc gtgaaagtgt ggaagtgact cgaggaattc actcctcagg
     5281 tgc
//
